Multi-tissue patterning drives anterior morphogenesis of the C. elegans embryo.

Complex structures derived from multiple tissue types are challenging to study in vivo, and our knowledge of how cells from different tissues are coordinated is limited. Model organisms have proven invaluable for improving our understanding of how chemical and mechanical cues between cells from two different tissues can govern specific morphogenetic events. Here we used Caenorhabditis elegans as a model system to show how cells from three different tissues are coordinated to give rise to the anterior lumen. While some aspects of pharyngeal morphogenesis have been well-described, it is less clear how cells from the pharynx, epidermis and neuroblasts coordinate to define the location of the anterior lumen and supporting structures. Using various microscopy and software approaches, we define the movements and patterns of these cells during anterior morphogenesis. Projections from the anterior-most pharyngeal cells (arcade cells) provide the first visible markers for the location of the future lumen, and facilitate patterning of the surrounding neuroblasts. These neuroblast patterns control the rate of migration of the anterior epidermal cells, whereas the epidermal cells ultimately reinforce and control the position of the future lumen, as they must join with the pharyngeal cells for their epithelialization. Our studies are the first to characterize anterior morphogenesis in C. elegans in detail and should lay the framework for identifying how these different patterns are controlled at the molecular level.

Estimulaçäo neural transcutânea e estimulaçäo eletromagnética como terapias de suporte para desordens craniomandibulares: uma avaliaçäo eletromiográfica / Transcutaneous electrical neural stimulation and electromagnetic simulation as support therapy to craniomandibular disorders: an electromyographic evaluation

Várias modalidades terapêuticas vêm sendo utilizadas no tratamento das desordens craniomandibulares. No presente estudo buscou-se avaliar eletromiograficamente duas dessas terapias: estimulaçäo neural transcutanea e estimulaçäo eletromagnética em 10 pacientes portadores de desordens craniomandibulares. Os resultados obtidos foram uma diminuiçäo no grau de fadiga muscular e um aumento na atividade muscular (músculos temporal anterior e masseter em máxima contraçäo voluntária), após as sessöes de aplicaçäo