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Anal Biochem ; 632: 114391, 2021 11 01.
Artigo em Inglês | MEDLINE | ID: mdl-34562467

RESUMO

RNA represents a vibrant area of research and many studies use techniques that require large amounts of purified RNA. One common purification method involves slicing a section of a polyacrylamide gel containing the RNA of interest and eluting the RNA out of the gel using electroelution. Various electroeluter models are available but sometimes a given model becomes discontinued, compelling researchers to choose a different model. Here, we have compared two electroeluters with different chamber designs for their ability to recover RNA from gel pieces. Our results show that both electroeluters are effective and recover comparable amounts of purified RNA.


Assuntos
Resinas Acrílicas/química , Bacillus subtilis/química , Eletroforese em Gel de Poliacrilamida , Mycobacterium tuberculosis/química , RNA Bacteriano/isolamento & purificação , RNA Bacteriano/química
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