RESUMO
Yeast 3-phosphoglycerate kinase (ATP:3-phospho-D-glycerate 1-phospho-transferase, EC 2.7.2.3) is inactivated by phenylglyoxal. Loss of activity correlates with the modification of two arginyl residues, both of which are protected by all of the substrates. The modification is not accompanied by any significant conformational change as determined by optical rotatory dispersion. Ultraviolet difference spectrophotometry indicates that the inactivated enzyme retains its capacity for binding the nucleotide substrates whereas the spectral perturbation characteristic of 3-phosphoglycerate binding is abolished in the modified enzyme. The data suggest that at least one of the two essential arginyl residues is located at or near the 3-phosphoglycerate binding site. A likely role of this residue could be its interaction with the negatively charged phosphate or carboxylate groups of 3-phosphoglycerate.
Assuntos
Arginina , Fosfoglicerato Quinase , Sítios de Ligação , Cinética , Fosfoglicerato Quinase/metabolismo , Ligação Proteica , Saccharomyces cerevisiae/enzimologia , Espectrofotometria UltravioletaRESUMO
A comparison of specific antibodies induced by unfolded actins modified either by oxidation or by arylation of lysine residues was reported. We have focused our work on binding properties with filamentous actin and located its preferential antigenic sites for the anti-arylated-actin antibodies in the C-part of the molecule. An interference of anti-oxidized actin antibodies upon actin polymerisation has also been reported.
Assuntos
Actinas/imunologia , Anticorpos , Epitopos , Actinas/metabolismo , Animais , Complexo Antígeno-Anticorpo , Imunodifusão , Cinética , Substâncias Macromoleculares , Oxirredução , Conformação Proteica , Coelhos , Radioimunoensaio , Relação Estrutura-AtividadeRESUMO
Titin is a giant protein which extends from Z-line to M-line in striated muscles. We report here the purification of a 150-kDa titin fragment, obtained after V8 protease treatment of myofibrils. This polypeptide was located at the N1-line level, in a titin part known to exhibit stiff properties correlated to an association with actin. By solid or liquid phase binding assays and cosedimentation, we have clearly demonstrated a direct, saturable and relative high affinity binding of the native titin fragment to F-actin. The 150-kDa titin fragment was also shown to accelerate actin polymerization. Furthermore, the actin-titin interaction was found to be inhibited by phosphoinositides.
Assuntos
Actinas/fisiologia , Proteínas Musculares/metabolismo , Fosfatidilinositol 4,5-Difosfato/metabolismo , Proteínas Quinases/metabolismo , Animais , Conectina , Proteínas Musculares/isolamento & purificação , Polímeros , Proteínas Quinases/isolamento & purificação , CoelhosRESUMO
The effect of Mg2+ ions on the conformation of G-actin and in particular on the accessibility of its antigenic regions has been tested. Experiments were performed with G-actin coupled to Sepharose 4B which was, therefore, maintained in the monomeric state. The results presented her show that the 2mM MgCl2-perturbed antigenic site is located in a central region of the actin sequence.
Assuntos
Actinas/metabolismo , Epitopos/análise , Magnésio/farmacologia , Serina Endopeptidases , Actinas/imunologia , Animais , Endopeptidases/metabolismo , Músculos/análise , Naftalenossulfonatos , Fragmentos de Peptídeos/análise , Conformação Proteica/efeitos dos fármacos , Coelhos , Dodecilsulfato de SódioRESUMO
The effect of 7-chloro-4-nitrobenzofurazan on yeast 3-phosphoglycerate kinase causes a modification of one tyrosyl residue concomitantly with a total loss of activity of the enzyme. The modification is not accompanied by any significant conformational change. A total protection against inactivation is observed with the substrates : furthermore, AMP, tripolyphosphate and pyrophosphate afford an effective protection. At pH 9, a shift in the absorbance spectrum of the tyrosine O-nitrobenzofurazan derivative of 3-phosphoglycerate kinase is observed. It can be related to the transfer of the reagent from tyrosine to lysine. The N-nitrobenzofurazan derivative is also completely inactive. It is concluded that a lysine residue is located close to the essential tyrosyl residue.
Assuntos
Lisina/análise , Fosfoglicerato Quinase/metabolismo , Tirosina/análise , 4-Cloro-7-nitrobenzofurazano/metabolismo , Sequência de Aminoácidos , Sítios de Ligação/efeitos dos fármacos , Cinética , Nitrobenzenos/farmacologia , Saccharomyces cerevisiae/enzimologiaRESUMO
Two fundamental properties of monomeric actin were examined in this study, ie its interaction with DNase-I, and the inhibition of endonuclease activity consecutive to the association of the two molecules. In particular, the topological independence between catalytic site of DNase-I and interface with actin, structural changes in actin monomer and the absence of conformational changes in DNase-I were described. We demonstrated a loss of flexibility of antigenic structures in actin subdomain I (ie epitopes 18-28 and 95-105) as well as modification in the exposure of Cys10 and Cys374 after DNase-I binding. Furthermore, the conformational changes induced by DNase-I into the actin molecule weakened the interaction of CapZ to its binding site located in the C-terminal region of actin monomer. These structural changes were time-dependent. When actin was cleaved in the DNase-I binding loop (sequence 38-52) at position 42 by E coli A2 strain protease, a tight DNase-I binding to split actin and the conformational changes were still observed, whereas the DNase-I inhibition activity was completely abolished. Finally, when we substitute Ca2+ by Mg2+ (ATP-Mg2+ monomeric actin) which induces a tighter conformation of actin and partially restores the inhibitory ability of split actin, long-range conformational effects of DNase-I are prevented and the ternary complex DNase-I-actin-CapZ is obtained.
Assuntos
Actinas/química , Desoxirribonuclease I/química , Proteínas dos Microfilamentos , Proteínas Musculares/química , Animais , Bass , Proteína de Capeamento de Actina CapZ , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Magnésio/farmacologia , Ligação Proteica , Estrutura Secundária de Proteína , Coelhos , Espectrometria de Fluorescência , Relação Estrutura-AtividadeRESUMO
The specific properties of sera elicited by various native, unfolded or chemically modified actins were compared to provide a means of obtaining high titres of antibodies directed against the N-terminal (1-39) or the central regions of the actin sequence. The antigenic structure of the N-terminal region of actin was analyzed. It has at least 2 discrete epitopes, one of which appears to be species-specific and is composed of the hydrophilic N-terminal heptapeptide sequence.
Assuntos
Actinas/imunologia , Sequência de Aminoácidos , Animais , Especificidade de Anticorpos , Epitopos , Formiatos , Oxirredução , Fragmentos de Peptídeos/imunologia , Conformação Proteica , Coelhos , Radioimunoensaio , SolubilidadeRESUMO
A competitive ELISA has been used to titrate skeletal muscle and total actins in human plasma. Specific antibodies directed against the variable N-terminal 1-7 sequence and conserved sequences respectively were used. The N-terminus of actin appears to be accessible in native and brevin-complexed actins. The skeletal muscle actin isoform represents about 1% of the total circulating actin (mean: 50 micrograms/ml plasma), but is markedly increased after severe muscle tissue injuries.
Assuntos
Actinas/sangue , Anticorpos/imunologia , Ensaio de Imunoadsorção Enzimática , Proteínas dos Microfilamentos , Músculos/metabolismo , Actinas/imunologia , Actinas/metabolismo , Adulto , Especificidade de Anticorpos , Ligação Competitiva , Proteínas de Transporte/metabolismo , Estudos de Avaliação como Assunto , Gelsolina , Humanos , Pessoa de Meia-Idade , Músculos/imunologia , Ferimentos e Lesões/sangueRESUMO
Regulation of actin polymerization and depolymerization is essential for the functions of actin in non-muscle cells and is mediated by a large number of heterologous actin-binding proteins which questions their true impact on the polymerization process. As a model, we report here the modulating effect of monospecific antibody fragments (Fab) as in vitro effectors on actin polymerization kinetics. Polymerization curves were obtained through fluorescence measurements. They were fitted using analytical equations derived from classical models describing the actin polymerization process with the aim of identifying kinetic steps potentially altered by the effectors. The study was limited to three short segments bore by the 300-328 sequence which is located in actin subdomain 3 and implicated in one of the monomer-monomer interfaces. We observed that antibodies which inhibited actin polymerization reacted with both G- and F-actins, modulated both nucleation and elongation steps, enhanced actin monomer dissociation from the filament and apparently did not act as capping or sequestering proteins. Among the antibody populations specific for a restricted and selected sequence in subdomain 3 of actin (sequence 300-326), only those directed to epitopes located near Met 305 and 325 were effective. In contrast, antibodies directed towards the alpha-helix located between the two preceding epitopes had no effect. All the results analyzed here emphasize the important role of some discrete regions and their conformational state in regulation of the interconversion between monomeric and polymeric actins which could be controlled in different ways by the various actin-binding proteins.
Assuntos
Actinas/química , Actinas/imunologia , Animais , Sítios de Ligação , Fenômenos Biofísicos , Biofísica , Fenômenos Químicos , Físico-Química , Epitopos/química , Técnicas In Vitro , Magnésio/química , Modelos Químicos , Estrutura Molecular , Moluscos , Polímeros/química , Conformação Proteica , CoelhosRESUMO
The aim of this study was to investigate the possibility of an interaction of yeast 3-phosphoglycerate kinase with negatively charged carriers such as polyanionic agents or a polarized electrode. Various polyanions were found to promote enzyme aggregation as judged by ultracentrifugation measurements and chemical modification. The data obtained suggest that these interactions are mediated through the N-terminal domain of the protein. However, the most striking property of 3-phosphoglycerate kinase described here is concerned with its significant dipolar moment as evidenced by electrocapillary measurements, which allows an orientation of the macromolecule in an electric field. Further, the enzyme could be absorbed by a negatively charged surface, first by hydrophobic links and then oriented perpendicularly to the surface. Therefore, the intrinsic properties of yeast 3-phosphoglycerate kinase agree with the formation of an enzyme-membrane complex and afford the ability for a specific orientation of the molecule at the lipid bilayer surface or in the cytoplasm.
Assuntos
Ácido Ditionitrobenzoico/farmacologia , Nitrobenzoatos/farmacologia , Fosfoglicerato Quinase/metabolismo , Saccharomyces cerevisiae/enzimologia , Sulfatos de Condroitina/farmacologia , Estabilidade de Medicamentos , Concentração Osmolar , Ácido Poliglutâmico/farmacologia , Polifosfatos/farmacologia , Polivinil/farmacologia , Relação Estrutura-AtividadeRESUMO
DNAse I-actin complex formation is studied in the presence of different anti actin antibody populations. The binding of DNAse I to actin is shown to be affected by antibodies specific to a central region in actin sequence (168-226). The C- and N-extremities of actin are shown to be in spatial proximity at the surface of the actin monomer and far from the binding area of DNAse I.
Assuntos
Actinas/imunologia , Desoxirribonuclease I/imunologia , Sequência de Aminoácidos , Animais , Anticorpos/imunologia , Sítios de Ligação , Imunoquímica , CoelhosRESUMO
The interaction of two different anti-actin antibody populations with the myosin subfragment 1-F-actin rigor complex has been studied. In contrast with the 1-7 sequence, the 18-28 sequence appears to be strongly implicated in the contact area of the myosin head on the actin polypeptide chain.
Assuntos
Actinas/metabolismo , Miosinas/metabolismo , Fragmentos de Peptídeos/metabolismo , Sequência de Aminoácidos , Animais , Reações Antígeno-Anticorpo , Sítios de Ligação , Imunoquímica , Subfragmentos de Miosina , Miosinas/imunologia , Fragmentos de Peptídeos/imunologia , CoelhosRESUMO
All during fish postmortem evolution, structural muscle proteins are targets for various proteases. During the prerigor period (24 hours at 4 degrees C for sea bass), cytoskeletal proteins are affected by the first proteolytic events. These cleavages disrupt connections between myofibrils and the extracellular matrix, induce segmentation of myofibril cores, and modify the rheological properties of tissue. Dystrophin, a cytoskeletal actin-binding protein, is a relevant in situ marker for muscular proteolysis in the prerigor period. The immunodetection of dystrophin allowed the monitoring of early proteolysis during fish storage. Using antidystrophin antibodies directed toward the carboxy-terminal region, a highly sensitive domain exposed to calpain activity, we showed that proteolysis kinetics are strongly influenced by the muscular lipid content. In particular, comparison between low-fat diets (11.3% lipid) and high-fat diets (30% lipid), used during sea bass farming (90 days), revealed a faster proteolysis rate during the first 8 hours of storage at 0 degrees C with the high-fat diet. The origin of this faster proteolysis is discussed on the basis of a possible activation or translocation of calpains related to lipid accumulation in muscle fibers and cytoskeleton alterations.
RESUMO
We have compared the functional properties of CapZ from fish white skeletal muscle with those of CapZ from chicken muscle. CapZ is a heterodimer, which enhances actin nucleation and inhibits the depolymerization process by binding to the barbed ends of microfilaments. Here, we report the interaction of CapZ not only with F-actin, but also with monomeric actin. The affinity of sea bass CapZ for G-actin estimated by enzyme-linked immunosorbent assay (ELISA) was in the microM range. This association was PIP2 dependent. Binding contacts with the barbed end of actin were delimited by both ELISA and fluorescence approaches. One site (actin sequence 338-348) was located in a helical region of the subdomain 1, region already implicated in the interaction with other actin binding proteins such as gelsolin. Another site implicates the C-terminal region (sequence 360-372) of actin. Finally, the partial competition of antibodies directed against CapZ alpha or beta-subunits towards CapZ interaction with actin filaments suggests both subunits participate in the complex with actin.
Assuntos
Actinas/metabolismo , Bass/metabolismo , Proteínas dos Microfilamentos/metabolismo , Proteínas Musculares/metabolismo , Músculo Esquelético/metabolismo , Fosfatidilinositóis/metabolismo , Animais , Proteína de Capeamento de Actina CapZ , Galinhas , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Imunoglobulina G/imunologia , Peso Molecular , Fragmentos de Peptídeos/metabolismo , CoelhosRESUMO
The ubiquity of actin, like the functional diversity of many associated proteins, raises a question concerning diversification of motility mechanisms and thus the emergence of an elementary functional system. Our aim was to investigate, in particular, mobiles prokaryotics cells as Synechocystis lacking cilia and flagella, search for actin essential properties and then locate the molecular behaviours. Here we report the presence and purification of a 56-kDa (apparent molecular weight) prokaryotic protein that polymerizes to form filaments, activates myosin Mg(++)-ATPase activity, inhibits DNase-1 activity and affords close antigenic homology to skeletal actin. This protein was found to be associated with thylakoid membranes and extracted in the presence of Triton X-100.
Assuntos
Actinas/fisiologia , Proteínas de Bactérias/química , Proteínas de Bactérias/metabolismo , Cianobactérias/química , Animais , Proteínas de Bactérias/imunologia , Movimento Celular/fisiologia , Desoxirribonuclease I/metabolismo , Eletroforese em Gel de Poliacrilamida/métodos , Músculo Esquelético/química , Miosinas/metabolismo , Polímeros , CoelhosRESUMO
Actin, together with associated proteins, such as myosin, cross-linking or capping proteins, has been observed in all eukaryotic cells. Presence of actin or actin-like proteins has also been reported in prokaryotic organisms belonging to the cyanobacteria. Our aim was first to extend the characterization of an actin-like protein to another prokaryotic cell, i.e. Spirulina, then to compare the antigenic reactivity of this new protein with that of Synechocystis and skeletal actins. We observed that some of the conserved antigenic epitopes corresponded to actin regions known to interact with cross-linking proteins. We also report for the first time that alpha-actinin and filamin purified from chicken gizzard both interact with a prokaryotic actin-like protein. Finally, we searched for the occurrence of a cross-linking protein in these cyanobacteria and identified a 105-kDa protein as an alpha-actinin-like protein using specific antibodies.
Assuntos
Actinina/metabolismo , Actinas/metabolismo , Cianobactérias/metabolismo , Actinina/genética , Actinina/imunologia , Actinas/genética , Actinas/imunologia , Sequência de Aminoácidos , Animais , Antígenos de Bactérias/genética , Proteínas de Bactérias/genética , Proteínas de Bactérias/imunologia , Proteínas de Bactérias/metabolismo , Reações Cruzadas , Reagentes de Ligações Cruzadas , Cianobactérias/genética , Cianobactérias/imunologia , Epitopos/genética , Evolução Molecular , Dados de Sequência Molecular , Proteínas Musculares/metabolismo , Especificidade da EspécieRESUMO
Fish alpha-actinin purified from sea-trout and bass white muscle by means of two different extraction procedures was used to investigate the eventual presence of different muscle isoforms in Z-disks. These fish alpha-actinins have the same apparent molecular weight (100 kDa) and the same isoelectric point (pI = 5.6), and also have a total antigenic identity towards anti-bass and anti-chicken alpha-actinin antibodies, suggesting a single molecular species. The role of fish alpha-actinin as an anchorage site for thin actin filaments and elastic titin filaments in Z-bands was studied. Despite conservation of the actin-binding site, fish alpha-actinin has a better actin-binding ability (kD = 0.3 microM) than chicken smooth muscle alpha-actinin (kD = 1.6 microM). Several other structural and functional characteristics of fish alpha-actinin were also studied: conservation of sequence and domain structure, the role of divalent ions (Ca2+, Mg2+) and the dielectric constant of the medium in alpha-actinin-actin interaction. Although the reason for fish white muscle alpha-actinin's close affinity to actin was not clearly established, our results suggested that the physicochemical environment of the Z-filaments in Z-disks might be crucial.