RESUMO
Hyperglycemia is associated with impaired endothelium-dependent dilation that is due to quenching of NO by superoxide (O(2)(. -)). In small coronary arteries (CAs), dilation depends more on smooth muscle hyperpolarization, such as that mediated by voltage-gated K(+) (Kv) channels. We determined whether high glucose enhances O(2)(.-) production and reduces microvascular Kv channel current and functional responses. CAs from Sprague-Dawley rats were incubated 24 hours in medium containing either normal glucose (NG, 5.5 mmol/L D-glucose), high glucose (HG, 23 mmol/L D-glucose), or L-glucose (LG, 5.5 mmol/L D-glucose and 17 mmol/L L-glucose). O(2)(.-) production was increased in HG arteries. Whole-cell patch clamping showed a reduction of 4-aminopyridine (4-AP)-sensitive current (Kv current) from smooth muscle cells of HG CAs versus NG CAs or versus LG CAs (peak density was 9.95+/-5.3 pA/pF for HG versus 27.8+/-6.8 pA/pF for NG and 28.5+/-5.2 pA/pF for LG; P<0.05). O(2)(.-) generation (xanthine+xanthine oxidase) decreased K(+) current density, with no further reduction by 4-AP. Partial restoration was observed with superoxide dismutase and catalase. Constriction to 3 mmol/L 4-AP was reduced in vessels exposed to HG (13+/-5%, P<0.05) versus NG (30+/-7%) or LG (34+/-4%). Responses to KCl and nifedipine were not different among groups. Superoxide dismutase and catalase increased contraction to 4-AP in HG CAs. This is the first direct evidence that exposure of CAs to HG impairs Kv channel activity. We speculate that this O(2)(.-)-induced impairment may reduce vasodilator responsiveness in the coronary circulation of subjects with coronary disease or its risk factors.
Assuntos
Vasos Coronários/efeitos dos fármacos , Glucose/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Canais de Potássio/fisiologia , 4-Aminopiridina/farmacologia , Análise de Variância , Animais , Antioxidantes/farmacologia , Catalase/farmacologia , Vasos Coronários/metabolismo , Vasos Coronários/fisiologia , Relação Dose-Resposta a Droga , Eletrofisiologia , Fluorescência , Técnicas In Vitro , Ativação do Canal Iônico/fisiologia , Masculino , Microscopia de Vídeo , Músculo Liso Vascular/citologia , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/fisiologia , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley , Superóxido Dismutase/farmacologia , Superóxidos/metabolismo , Vasoconstrição/efeitos dos fármacosRESUMO
We proposed earlier that voltage-dependent calcium (Ca2+) current is altered in single azygos venous cells from Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). In this study, the effects of different intracellular concentrations of ethylene glycol-bis-N,N,N',N',-tetraacetic acid (EGTA) on Ca2+ currents were investigated. Vascular muscle cells from SHR and WKY rats were equilibrated with pipette solution containing 0.1 mM or 10 mM EGTA. Increasing the EGTA concentration from 0.1 to 10 mM in SHR vascular cells significantly enhanced the peak amplitude of the longer lasting (L) current from 87 +/- 12 pA to 152 +/- 8 pA, while the transient (T) current amplitude was not significantly different (52 +/- 7 pA and 36 +/- 7 pA, respectively). In WKY rat vascular muscle cells, the amplitudes of the T and L currents were not significantly different with the same comparison of intracellular EGTA concentrations. These observations suggest that relatively low intracellular Ca2+ concentrations can more strongly modulate Ca2+ current through the L channel in SHR than WKY rat vascular muscle cells.
Assuntos
Canais de Cálcio/fisiologia , Hipertensão/fisiopatologia , Músculo Liso Vascular/fisiopatologia , Ratos Endogâmicos SHR/fisiologia , Ratos Endogâmicos/fisiologia , Animais , Cálcio/fisiologia , Células Cultivadas , Ácido Egtázico/farmacologia , Hipertensão/genética , Técnicas In Vitro , Ratos , Ratos Endogâmicos WKYRESUMO
Rat arterial muscle cells show an elevated Ca(2+)-dependent K+ efflux during the established phase of hypertension. This association of enhanced K+ efflux with high arterial pressure implies that changes of in vivo blood pressure can alter the level of K+ channel current in arterial membranes. We directly tested this hypothesis by comparing K+ current density between patch-clamped aortic muscle membranes of normotensive Wistar-Kyoto (WKY) rats, spontaneously hypertensive rats (SHR), and SHR treated with the angiotensin-converting enzyme inhibitor ramipril (3.5 mg/kg per day PO) to normalize blood pressure. Peak macroscopic K+ current was measured during progressive depolarizing steps (10 mV) from -60 and +60 mV in cells dialyzed with pipette solution containing 10(-6) mol/L calcium to amplify Ca(2+)-dependent K+ current. With the use of this approach, maximum K+ current density in aortic muscle membranes of untreated SHR was 2.6-fold higher than in untreated WKY rats (SHR, 31 +/- 3 pA/pF; WKY, 12 +/- 1 pA/pF) and was predominantly blocked by 2 mmol/L tetraethylammonium. K+ current density in SHR aortic membranes was unchanged after 1 week of ramipril therapy, but it was reduced 42% (to 18 +/- 1 pA/pF) after 2 weeks of treatment. Parallel tension-recording studies showed that untreated SHR aortic segments but not aortic segments from WKY rats or ramipril-treated SHR constricted strongly after block of Ca(2+)-dependent K+ channels by tetraethylammonium. Our findings imply that Ca(2+)-dependent K+ current density in arterial muscle membranes shows a positive correlation with chronic arterial blood pressure levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Aorta/metabolismo , Hipertensão/metabolismo , Hipertensão/fisiopatologia , Canais de Potássio/metabolismo , Animais , Pressão Sanguínea/efeitos dos fármacos , Eletrofisiologia , Hipertensão/tratamento farmacológico , Músculo Liso Vascular/metabolismo , Bloqueadores dos Canais de Potássio , Canais de Potássio/fisiologia , Ramipril/uso terapêutico , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Tetraetilamônio , Compostos de Tetraetilamônio/farmacologiaRESUMO
Hypertension has been linked to an impaired dilator function of the coronary microvascular endothelium in vivo. However, the profile and mechanism of this dysfunction remain obscure. Thus, this study compared diameter responses to acetylcholine (ACH), bradykinin (BKN), and substance P (SP) between coronary microvessels (i.d.=106+/-4 microm) dissected from left ventricles of normotensive and hypertensive Dahl rats (Dahl-NT and Dahl-HT, respectively). Vessels were cannulated and pressurized on glass pipettes at 80 mm Hg, and internal diameters were monitored by videomicroscopy. Coronary microvessels from Dahl-NT and Dahl-HT showed similar dilator responses to ACH (100 pmol/L to 10 micromol/L), with maximal diameter increases of 63+/-5 microm and 63+/-7 microm, respectively (n=31,17). However, only vessels from Dahl-NT showed dilator responses to SP (10 fmol/L to 1 nmol/L) and BKN (100 fmol/L to 10 nmol/L). All dilator responses persisted after N-nitro-L-arginine (10 micromol/L) or indomethacin (10 micromol/L), but were blunted after inhibition of cytochrome P450 by 10 micromol/L octadecynoic acid (n=6-8). These results suggest that: (1) coronary microvessels from Dahl-HT show a unique pattern of endothelial impairment, whereby ACH-induced relaxations persist at a time when dilator responses to SP and BKN are severely blunted, and (2) a cytochrome P450 product, rather than nitric oxide or prostacyclin, may partly mediate the vasodilator responses to ACH, SP and BKN.
Assuntos
Vasos Coronários/fisiopatologia , Endotélio Vascular/fisiopatologia , Hipertensão/fisiopatologia , Microcirculação/fisiopatologia , Acetilcolina/farmacologia , Animais , Bradicinina/farmacologia , Vasos Coronários/efeitos dos fármacos , Vasos Coronários/fisiologia , Inibidores das Enzimas do Citocromo P-450 , Dieta Hipossódica , Relação Dose-Resposta a Droga , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/fisiologia , Ácidos Graxos Insaturados/farmacologia , Técnicas In Vitro , Indometacina/farmacologia , Masculino , Microcirculação/efeitos dos fármacos , Microcirculação/fisiologia , Nitroarginina/farmacologia , Ratos , Ratos Endogâmicos , Sódio na Dieta , Substância P/farmacologia , Vasodilatação/efeitos dos fármacosRESUMO
A high salt diet often is regarded as an accessory risk factor in hypertension, coincidental to the deleterious effect of high blood pressure on vasodilator function. The aim of this study was to determine whether short-term ingestion of a high salt diet per se impairs vasodilator function in the cerebral circulation independent of blood pressure changes. Adult Sprague-Dawley rats were fed a normal salt (0.8%) or high salt (4%) diet for 3 days. Mean arterial pressures were similar in the normal and high salt groups (123+/-2 and 125+/-2 mm Hg, respectively). Subsequently, the responses of the in situ pial arterioles to acetylcholine, iloprost, and sodium nitroprusside were determined in cranial windows using intravital videomicroscopy. Pial arterioles of rats fed normal and high salt diets showed similar resting diameters of 69+/-2 and 72+/-3 microm, respectively, but their reactivity patterns to vasodilator stimuli were markedly different. Arterioles of rats fed a normal salt diet dilated progressively up to 17+/-3% in response to the endothelium-dependent agent acetylcholine (10(-9) to 10(-6) mol/L) and dilated by 22+/-2% in response to the prostaglandin I2 receptor agonist iloprost (3x10(-11) mol/L). In contrast, pial arterioles of rats fed a high salt diet constricted by 4+/-3% and 8+/-2% in response to acetylcholine and iloprost, respectively. Sodium nitroprusside (10(-6) mol/L), a nitric oxide donor, dilated pial arterioles of rats fed low and high salt diets by a similar amount (19+/-3% and 16+/-2%, respectively), suggesting that signaling mechanisms for dilation distal to the vascular smooth muscle membrane were intact after high salt intake. These results provide the first evidence that the short-term ingestion of a high salt diet may severely impair the vasodilator function of the in situ cerebral microcirculation independent of blood pressure elevation.
Assuntos
Circulação Cerebrovascular/efeitos dos fármacos , Endotélio Vascular/patologia , Cloreto de Sódio na Dieta/administração & dosagem , Vasodilatação/efeitos dos fármacos , Animais , Artérias Cerebrais/efeitos dos fármacos , Artérias Cerebrais/patologia , Circulação Cerebrovascular/fisiologia , Iloprosta/farmacologia , Masculino , Ratos , Ratos Sprague-Dawley , Receptores de Epoprostenol , Receptores de Prostaglandina/antagonistas & inibidores , Receptores de Prostaglandina/fisiologia , Vasodilatadores/farmacologiaRESUMO
Pressurization of isolated arteries may result in Ca(2+)-dependent contraction and membrane depolarization. Because the open state probability of some vascular muscle K+ channels is augmented by rises in cytosolic Ca2+ and membrane depolarization, we investigated the possibility that increases in intraluminal pressure activate K+ channels in isolated, perfused rat saphenous arteries. Stepwise increases in intraluminal pressure from 5 to 205 mm Hg resulted in increasing, active arterial contraction, measured as smaller diameters in physiological salt solution than in Ca(2+)-free solution. Addition of 10 mM tetraethylammonium to the physiological salt solution to block arterial muscle K+ channels caused progressively greater diameter reductions at pressures above 25 mm Hg. Microelectrode measurements of membrane potential showed that tetraethylammonium depolarized arterial muscle more at 105 mm Hg (16 +/- 1 mV) than at 25 mm Hg (10 +/- 1 mV). The sensitivity of K+ current to tetraethylammonium was also demonstrated in patch-clamped vascular muscle cells from the same arteries. Peak whole-cell K+ current was suppressed 47% and 79% by 1 and 10 mM tetraethylammonium, respectively. This same current was enhanced 3.6-fold by the Ca2+ ionophore A23187 (10 microM), suggesting a Ca2+ dependence. We conclude that increases in intraluminal pressure progressively activate tetraethylammonium-sensitive K+ channels in the arterial muscle membrane. This can serve as a negative feedback mechanism to limit pressure-induced arterial constriction.
Assuntos
Pressão Sanguínea , Perna (Membro)/irrigação sanguínea , Potássio/fisiologia , Animais , Artérias/efeitos dos fármacos , Artérias/inervação , Artérias/fisiologia , Calcimicina/farmacologia , Cálcio/metabolismo , Citosol/metabolismo , Eletrofisiologia , Endotélio Vascular/fisiologia , Membranas/fisiologia , Canais de Potássio/efeitos dos fármacos , Canais de Potássio/fisiologia , Ratos , Ratos Endogâmicos , Simpatectomia Química , Tetraetilamônio , Compostos de Tetraetilamônio/farmacologia , Vasoconstritores/farmacologiaRESUMO
Sympathetic neural activation of vascular smooth muscle beta-receptors induces membrane hyperpolarization and arterial relaxation. This response, which likely is mediated by the Gs protein-adenylyl cyclase-cyclic AMP signaling cascade, is reduced in some hypertensive animal models and in human essential hypertension. Since reduced beta-receptor-mediated vasodilation is a potential mechanism for enhanced arterial resistance, this study was designed to identify which step (or steps) in the beta-receptor signaling cascade is altered in hypertension. Transmembrane potentials were recorded in situ in small first-order arterioles and venules of cremaster muscle from hypertensive, reduced renal mass rats and normotensive, sham-operated controls. Vascular muscle cells in arterioles and venules of hypertensive rats were 5-7 mV more depolarized than in respective vessels of control rats during superfusion with physiological salt solution. Hyperpolarization and depolarization responses were reduced in hypertensive rats during superfusion with a beta-receptor agonist and antagonist, respectively, suggesting attenuated beta-receptor responsiveness compared with normotensive rats. Furthermore, direct activation of Gs protein by 10 ng/mL cholera toxin did not affect arterial or venous transmembrane potential in hypertensive rats, but hyperpolarized arterial and venous vascular muscle in normotensive controls by 17 mV. However, when the Gs protein-adenylate cyclase coupling step of the beta-receptor cascade was bypassed by using 10(-5) M forskolin to directly activate adenylate cyclase, arterial and venous vascular muscle of hypertensive rats hyperpolarized by 25-27 mV.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Hipertensão/fisiopatologia , Receptores Adrenérgicos beta/fisiologia , Agonistas Adrenérgicos beta/farmacologia , Antagonistas Adrenérgicos beta/farmacologia , Animais , Toxina da Cólera/farmacologia , Colforsina/farmacologia , Masculino , Potenciais da Membrana/efeitos dos fármacos , Músculo Liso Vascular/fisiopatologia , Nefrectomia/métodos , Ratos , Ratos Sprague-DawleyRESUMO
Potassium efflux through Ca2+-sensitive K+ channels (K[Ca] channels) is increased in arterial smooth muscle cells from hypertensive rats, but the molecular mechanism is unknown. The goal of this study was to compare the levels of K(Ca) channel current between aortic smooth muscle cells from adult Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) and then use Western blot methods and ribonuclease protection assays to examine the expression and mRNA levels for the K(Ca) channel in these same vascular tissues. Whole-cell patch-clamp methods indicated a larger component of K(Ca) channel current, sensitive to block by iberiotoxin (100 nmol/L), in single aortic smooth muscle cells from SHR compared with WKY. Subsequent Western blot analysis using a site-specific antibody (anti-alpha[913-926]) directed against the S9/S10 linker of the alpha-subunit of the K(Ca), channel revealed a 125-kD immunoreactive band in lanes loaded with either WKY or SHR aortic muscle membranes. The immunoreactive density of this band, which corresponded to the known molecular size of the alpha-subunit, was 2.2-fold greater in lanes loaded with aortic smooth muscle membranes from the hypertensive animals. However, despite this evidence for an increased expression and functional enhancement of K(Ca) channels in aortic smooth muscle membranes of SHR, ribonuclease protection assays with a 32P-labeled riboprobe targeted against the S9/S10 linker of the K(Ca) channel alpha-subunit revealed no difference in mRNA levels for the alpha-subunit between WKY and SHR aortic tissue. These findings provide initial evidence that (1) an increased expression of K(Ca) channels may be a mechanism for the enhanced K(Ca) current in aortic smooth muscle membranes of SHR, and (2) the upregulation of K(Ca) channels in arterial muscle membranes during hypertension, which is regarded as a homeostatic mechanism for buffering vascular excitability, may rely on posttranscriptional events.
Assuntos
Aorta/metabolismo , Hipertensão/metabolismo , Músculo Liso Vascular/metabolismo , Canais de Potássio/biossíntese , Transcrição Gênica , Animais , Aorta/fisiologia , Aorta/fisiopatologia , Cálcio/farmacologia , Potenciais da Membrana/efeitos dos fármacos , Músculo Liso Vascular/fisiologia , Músculo Liso Vascular/fisiopatologia , Técnicas de Patch-Clamp , Peptídeos/farmacologia , Canais de Potássio/efeitos dos fármacos , Canais de Potássio/fisiologia , RNA Mensageiro/biossíntese , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Ratos Sprague-Dawley , Valores de Referência , Venenos de Escorpião/farmacologiaRESUMO
This study was designed to investigate the role and regulation of arterial membrane K+ channels in hypertension. Aortic segments from normotensive Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) were suspended for isometric tension recording. In other experiments, proximal aortic segments (PS) (exposed to high pressure) and distal aortic segments (DS) (exposed to lower pressure) were removed from surgically coarcted Sprague-Dawley rats and similarly prepared. Aortas from SHR and PS dose-dependently contracted to the K+ channel blocker tetraethylammonium (TEA) (0.1-10 mM), and this contraction was abolished by preincubation with 0.1 microM nifedipine. In contrast, the same concentrations of TEA did not contract either WKY or DS aortas. Since block of K+ channels by TEA had a different effect on aortic segments exposed to high versus low blood pressure, we compared whole-cell K+ currents in isolated vascular cells from the same aortas. The reversal potentials of depolarization-induced outward currents in WKY, SHR, DS, and PS aortic cells showed a Nernst relation to external K+ concentration indicative of selective K+ permeability. TEA (1 and 10 mM) was equipotent in blocking these K+ currents in patch-clamped cells from all aortic preparations, suggesting that the lack of TEA-induced contractions in WKY and DS aortas was not due to an absence of TEA-sensitive K+ channels in these arterial membranes. However, when the Ca2+ ionophore A23187 (10 microM) was used to increase the level of cytosolic Ca2+ in patch-clamped cells, the K+ current density in SHR and PS aortic cells was twofold or more higher than in WKY and DS cells.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Cálcio/fisiologia , Contração Muscular/efeitos dos fármacos , Potássio/metabolismo , Compostos de Tetraetilamônio/farmacologia , Animais , Aorta , Calcimicina/farmacologia , Permeabilidade da Membrana Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/fisiologia , Nifedipino/farmacologia , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Tetraetilamônio , Compostos de Tetraetilamônio/antagonistas & inibidoresRESUMO
To explore the hypothesis that altered vascular muscle signal transduction may underlie some of the vascular changes observed in hypertensive models, we measured expression of GTP-binding protein (G protein) alpha-subunits, Gs, G(i), and Gq, in aortic muscle of reduced renal mass and sham-operated rats and proximal and distal aortic segments from rats with interrenal aortic coarctation (IR-AC). G protein expression was measured by immunoblot analysis. When we probed aortic muscle membrane with G(i) and Gq alpha-subunit antibodies, we identified 41- and 42-kD immunoreactive proteins, respectively. Three immunoreactive bands specific to Gs alpha-subunit antibody were resolved. Immunoreactive blot densities were compared. In aortic muscle membrane of reduced renal mass rats (blood pressure, 148 +/- 7 mm Hg), we found significantly reduced Gs and G(i) blot densities compared with sham-operated controls (blood pressure, 99 +/- 12 mm Hg). There were no differences in Gq blot densities between reduced renal mass and control rats. Gs and G(i) blot densities were significantly lower in IR-AC proximal aortic segments (carotid pressure, 165 +/- 5 mm Hg) and distal aortic segments (femoral pressure, 121 +/- 4 mm Hg) than in aortas of sham-operated controls. In contrast, Gq expression was significantly increased in the high-pressure proximal aortic segments compared with low-pressure distal aortic segments from IR-AC rats. Thus, altered G protein expression occurs in aortic muscle from nongenetic rat models of hypertension.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Aorta/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Hipertensão/metabolismo , Músculo Liso Vascular/metabolismo , Animais , Coartação Aórtica/metabolismo , Immunoblotting , Masculino , Membranas/metabolismo , Nefrectomia , Ratos , Ratos Sprague-DawleyRESUMO
This study was designed to measure the effect of ambient temperature (25 degrees C) on papaverine-induced relaxations in canine saphenous veins. Segments of vein were suspended in water-jacketed tissue baths at 37 degrees C, and isometric tension was recorded. After equilibration, veins were preconstricted by a median effective dose of norepinephrine 2 x 10(-6) mol/L at either 25 degrees C or 37 degrees C. Consequent dose-dependent relaxations showed that papaverine (10(-7) to 10(-3) mol/L was three times more potent as a dilator at 37 degrees C than at 25 degrees C, with half-maximal relaxations occurring at 2.2 x 10(-5) mol/L and 6.4 x 10(-5) mol/L, respectively. A 10(-4) mol/L dose of papaverine completely relaxed veins at 37 degrees C, whereas veins at 25 degrees C never fully relaxed even at ten times the standard concentration. In addition, the time for half-maximal relaxation with a 10(-4) mol/L dose of papaverine averaged 40 minutes at 25 degrees C compared with 22 minutes at 37 degrees C; this is indicative of a reduced relaxation rate at the lower temperature. These data show that papaverine is a slower and less potent dilator of canine saphenous veins at 25 degrees C than at 37 degrees C. This may have implications for the use of papaverine in the operating room, where it is usually applied at ambient temperature to reduce vasospasm of the saphenous vein during coronary artery bypass procedures.
Assuntos
Papaverina/farmacologia , Veia Safena/efeitos dos fármacos , Temperatura , Vasodilatação/efeitos dos fármacos , Animais , Cães , Relação Dose-Resposta a Droga , Papaverina/administração & dosagem , Papaverina/farmacocinética , Fatores de TempoRESUMO
Vasospasm of human saphenous vein grafts has been reported after aorta-coronary bypass operations. However, it is unknown whether veno-arterial grafts are inherently responsive to vasoconstrictor stimuli after implantation into the arterial circulation or whether their vasomotion is secondary to hemodynamic changes. Thus in this study we used in vitro methods to directly evaluate whether isolated human saphenous vein segments respond to vasoconstrictor agents at arterial pressure levels. External diameter and intraluminal flow were monitored in 12 human saphenous vein segments, which were perfused at 30 ml/min with physiologic salt solution at 90, 70, and 50 mm Hg. Increasing intraluminal pressure higher than 50 mm Hg or exposing the vein to Ca(2+)-free media did not increase vessel external diameter or intraluminal flow, which suggests that human saphenous veins were fully distended at pressures of 50 mm Hg or greater. However, all human saphenous veins were activated by a 1 mumol/L dose of norepinephrine at 50 mm Hg and dilated during subsequent intraluminal infusion of a 1 mumol/L dose of acetylcholine, showing intact vascular smooth muscle and endothelial cell function. In the same vessels, a 1 mumol/L concentration of 5-hydroxytryptamine constricted human saphenous veins by 19%, 22%, and 26% at intraluminal pressures of 90, 70, and 50 mm Hg, respectively, and reduced vessel flow by 6%, 24%, and 42% at the same pressure levels. Similarly, a 1 mumol/L concentration of norepinephrine constricted vessels pressurized at 90, 70, and 50 mm Hg by 9%, 12%, and 17%, respectively, and attenuated vessel flow by as much as 32%. We conclude that human saphenous vein segments are fully distended at perfusion pressures greater than 50 mm Hg, but can dynamically constrict to vasoactive agonists and regulate graft flow at intraluminal pressures as high as 90 mm Hg. Our findings in isolated human saphenous vein segments lend support to clinical observations that human saphenous vein grafts should be regarded as vasoactive conduits after implantation at arterial pressure levels.
Assuntos
Pressão Sanguínea , Veia Safena/fisiologia , Acetilcolina/farmacologia , Idoso , Humanos , Técnicas In Vitro , Masculino , Pessoa de Meia-Idade , Norepinefrina/farmacologia , Serotonina/farmacologia , Vasoconstritores/farmacologiaRESUMO
Venospasm of saphenous vein grafts may damage endothelial cells and compromise early and late graft performance. Hence it is desirable to identify and use storage solutions that minimize vascular spasm during vein preparation. In view of this, we initiated isometric tension-recording studies in isolated canine and human saphenous vein to evaluate the acute, vasoactive effects of two storage solutions, Plasma-Lyte solution and normal saline solution. In initial experiments, canine saphenous veins were mounted in tissue baths containing physiologic salt solution and tonically constricted by 2 x 10(-6) mol/L norepinephrine. The physiologic salt solution in the bath was then replaced by Plasma-Lyte solution or normal saline solution containing the same norepinephrine concentration, and changes in contraction amplitude were recorded for 90 minutes. Storage in Plasma-Lyte solution at 37 degrees C completely relaxed norepinephrine-activated canine saphenous vein within 20 minutes, whereas veins remained partially constricted in normal saline solution. Both Plasma-Lyte solution and normal saline solution relaxed canine saphenous vein less at room temperature (25 degrees C) than at 37 degrees C, implying that warming of storage solutions in the operating room may promote graft dilation. To identify the mechanism by which Plasma-Lyte solution induced relaxation, we replaced its putative vasodilator components of gluconate and acetate with NaCl, but this alteration did not reduce relaxation induced by Plasma-Lyte solution. However, adding 1.6 mmol/L CaCl2 to Plasma-Lyte solution completely reversed the venodilation, suggesting that the low Ca2+ content of Plasma-Lyte solution confers its relaxant action. Finally, we tested the vasoactive effect of Plasma-Lyte solution on human saphenous vein obtained by discard from coronary bypass operations. Plasma-Lyte solution at 37 degrees C effectively dilated norepinephrine-activated human saphenous vein, inducing complete relaxation within 20 minutes. On this basis, we recommend the use of Plasma-Lyte solution as a venodilating storage solution during coronary bypass operations to optimize vein graft relaxation before implantation.
Assuntos
Soluções Cardioplégicas/farmacologia , Veia Safena/efeitos dos fármacos , Cloreto de Sódio/farmacologia , Preservação de Tecido/métodos , Vasodilatadores/farmacologia , Acetatos/química , Acetatos/farmacologia , Animais , Soluções Cardioplégicas/química , Ponte de Artéria Coronária , Cães , Gluconatos/química , Gluconatos/farmacologia , Humanos , Técnicas In Vitro , Cloreto de Magnésio/química , Cloreto de Magnésio/farmacologia , Cloreto de Potássio/química , Cloreto de Potássio/farmacologia , Veia Safena/transplante , Acetato de Sódio , Cloreto de Sódio/química , Vasodilatação/efeitos dos fármacosRESUMO
Vasopressin (0.01-0.3 IU/ml) contracted isolated segments of rat basilar arteries from stroke-prone SHR (SP-SHR) and normotensive Wistar-Kyoto rats (WKY) unequally. Basilar arteries from SP-SHR were more responsive to vasopressin than were those from WKY when adrenergic nerve endings were present in both preparations. After destruction of adrenergic nerve endings by in vitro 6-hydroxydopamine treatment, the ED50 for vasopressin in both WKY and SP-SHR arteries decreased by a factor of three, indicating that the contractions caused by vasopressin were similarly modulated by prejunctional neurotransmitter release. However, only arteries from WKY showed prominent rhythmic relaxation-contraction cycles superimposed upon the vasopressin-induced tone. The tension cycles were 20-100 dyn in amplitude and occurred at 1-3 cycles/min. These tension oscillations of WKY were pronounced and obvious, sometimes amounting to as much tension as the underlying tonic contraction. Tension cycles could reflect a physiological contraction-relaxation phasing mechanism that fails to occur in basilar arteries of SP-SHR. The rhythmic activity was enhanced by K+-free solution and abolished by 30 mM K+ solution, suggesting that pacemaker changes in K+ conductance may underlie the WKY tension oscillations. It is suggested that the absence of rhythmic contractions in SP-SHR basilar arteries may be explained by greater activity of the electrogenic Na+-pump, which would tend to prevent the rhythmic oscillations in tension. These observations suggest that vasopressin has a differential action on basilar arteries of SP-SHR and WKY.
Assuntos
Artéria Basilar/efeitos dos fármacos , Lipressina/farmacologia , Contração Muscular/efeitos dos fármacos , Ratos Endogâmicos SHR/fisiologia , Ratos Endogâmicos/fisiologia , Ratos Endogâmicos WKY/fisiologia , Fibras Adrenérgicas/efeitos dos fármacos , Animais , Artéria Basilar/inervação , Relação Dose-Resposta a Droga , Feminino , Hidroxidopaminas/farmacologia , Masculino , Oxidopamina , RatosRESUMO
Potassium channels in cell membranes regulate cellular excitability, proliferation and growth. The central role of these ion channels in cell function has made them the targets for current drug treatment of hypertension, hair loss and diabetes, and also the subject of intense research for new drug therapies.
Assuntos
Canais de Potássio/efeitos dos fármacos , Diabetes Mellitus/tratamento farmacológico , Doenças do Cabelo/tratamento farmacológico , Humanos , Hipertensão/tratamento farmacológico , Canais de Potássio/fisiologiaRESUMO
The effects of vasopressin on membrane potential and tension were studied in isolated segments of basilar arteries from the University of Iowa colonies of normotensive inbred Kyoto-Wistar rats (WKY) and stroke-prone spontaneously hypertensive rats (SP-SHR). In the presence of vasopressin (0.01-0.3 IU/ml), basilar arteries from WKY, but not from SP-SHR, developed rhythmic contractions. These contractions were recorded in 13 of 14 WKY basilar arteries, were unaffected by pretreatment with 6-hydroxydopamine, and were characterized by 20-100 dyne oscillations in tension, occurring 1-3 cycles/min, and superimposed on the vasopressin-induced contraction (averaging 60 dynes at 0.01 IU/ml or 160 dynes at 0.3 IU/ml). However, resting membrane potentials were not different in SP-SHR vs. WKY at 37 degrees C, and both strains showed about the same (11 mV) depolarization by 0.1 IU/ml of vasopressin. The rhythmic contractions were enhanced by K+-free solution, and abolished in the presence of high K+ solution (30 mM), suggesting that active Na+-K+ transport may be involved in modulating the rhythmic activity. These findings are consistent with the hypothesis that the vasopressin-induced rhythmic contractions in WKY basilar arteries are at least partly dependent on a reduced activity of electrogenic Na+-K+ active transport in WKY as compared to SP-SHR.
Assuntos
Artéria Basilar/efeitos dos fármacos , Lipressina/farmacologia , Vasoconstrição/efeitos dos fármacos , Animais , Artéria Basilar/fisiologia , Engenharia Biomédica , Técnicas In Vitro , Potenciais da Membrana/efeitos dos fármacos , Potássio/metabolismo , Potássio/farmacologia , Ratos , Ratos Endogâmicos SHR , Ratos Endogâmicos WKY , Sódio/metabolismoRESUMO
The direct measurement of transmembrane calcium current in single vascular muscle cells has been accomplished recently using the whole-cell voltage-clamp technique. The small size of the vascular muscle cell and the proportionately smaller magnitude of its inward calcium current necessitate refined instrumentation, but also make the vascular muscle cell an ideal candidate for whole-cell voltage-clamp recording. Calcium current in vascular muscle cells appears to have some, but not all, characteristics in common with calcium currents similarly isolated in neuronal and cardiac cells, including voltage-dependent activation and steady-state inactivation of calcium current, the presence of two current types, and sensitivity to inorganic and organic calcium channel modulating drugs. Future voltage-clamp analysis of calcium currents in vascular muscle is needed to further our understanding of the control of the calcium channels in physiological and pathophysiological states.