Functional plasticity of carabids can presume better the changes in community composition than taxon-based descriptors.

Although the functional trait approach can facilitate the understanding of mechanisms that underline community responses to habitat alteration, only a few studies used this way on exploring the structure of insect assemblages compared to taxon-based analyses. We compared the descriptive power of medium-term effects (2014-2018) of forestry treatments in a temperate managed oak-dominated forest on taxon- vs. trait-based descriptors of ground beetle assemblages. The treatments included rotation forestry (partial preparation cutting, clear-cutting, retention-tree group, and mature closed forest as control) and continuous cover forestry (gap cutting) operations. The species composition was only slightly influenced by the treatments; on the ordination biplot, the control, retention tree group, and clear-cutting treatments formed relatively homogeneous groups, well separated from each other, while the others were scattered randomly in the ordination space. Over time, the species richness decreased in all treatments, but it was higher in the retention tree group treatment than in others in 2016 and 2017. The activity density also declined between years, but an immediate mass effect was revealed after the implementation of treatment types especially in the control, gap, and preparation cuts. We found that assemblages in the clear-cutting and retention-tree group had similar characteristics: high functional diversity; more open-habitat, generalist, and omnivore species and fewer carnivore species; while those in the control, gap, and preparation cutting groups had the opposite: lower functional diversity, more forest species, and more carnivorous species. Our findings will demonstrate that the simultaneous use of the two approaches will allow the most articulate understanding of the status of ground beetles assemblages in managed forests.

Female-female aggression and male responses to the two colour morphs of female common cuckoos.

Female-only colour polymorphism is rare in birds, but occurs in brood parasitic cuckoos (Cuculidae). Obligate brood parasites leave incubation and parental care to other species (hosts), so female-female interactions can play a role in how parasites guard critical resources (host nests) within their laying areas. The plumage of adult female common cuckoos (Cuculus canorus) is either rufous (typically rare) or grey (common), whereas adult male conspecifics are monochromatic (grey). In previous studies, hosts and conspecific males responded with less intensity toward the rare female morph in support of a negative frequency-dependent benefit of female plumage polychromatism. Here, we assessed responses of both conspecific females and males to vocal playbacks of female calls, coupled with one of two 3D models of the different morphs of female cuckoos. At our study population, the rufous female morph was as common as the grey morph; therefore, we predicted similarly high rates of conspecific responses in both treatments. Both female and male cuckoos responded to playbacks acoustically, which demonstrated the primary role of acoustic communication in social interactions amongst cuckoos. Following this, some cuckoos flew closer to the models to inspect them visually. As predicted, no significant differences were detected between the live cuckoos' responses toward the two colour morphs in this population. We conclude that dichromatism in female cuckoos evolved to serve one or more functions other than conspecific signalling.

Spent coffee ground as renewable energy source: Evaluation of the drying processes.

Spent coffee ground (SCG) is an environmental nuisance material, but, if appropriately processed it can be converted into pellets, and thus, used as an energy source. The moisture content of the final product should be below 10%, to ensure safe storage, and elimination of microorganism growth (particularly moulds). The present study aims to identify the optimal drying process for removing moisture from SCG and to investigate changes to the composition of SCG due to drying, at temperatures around 75 °C, so that the dried SCG to qualify as renewable energy source. Three drying processes were employed for SCG drying (with initial moisture content of about 65%): oven drying, solar drying and open air sun drying, while SCG samples were placed in aluminium trays with thicknesses of 1.25, 2.5 and 4 cm. Based on the experimental results for SCG samples with thickness 2.5 cm, the open air sun drying process required 10 h to reach final moisture content of 37%, while solar drying achieved 10% moisture content in 10 h and oven drying achieved 7% moisture content in 6 h. The solar drying process proved as the most advantageous, due to low energy requirements and adequate quality of dried SCG. Also, experiments indicated that SCG storage at "normal room conditions" resulted to equilibrium moisture content in SCG of 8%, regardless of the initial moisture content. Furthermore, instrumental analyses of the SCG, revealed changes to its composition for a number of chemical groups, such as aldehydes, ketones, phytosterols, alkaloids, lactones, alcohols, phenols, pyrans and furans, among others. It was also identified that the SCG colour was affected due to the drying process.

Application of multivariate statistical analysis using organic compounds: Source identification at a local scale (Napajedla, Czechia).

The study aimed to apply novel source classification tool for local scale air pollution assessment reducing the total number of organic compounds in the model. Samples of particulate matter (PM) were collected in the town of Napajedla (South-eastern Czech Republic) in 2016. The industrial sector of the town is represented by plastics processing and manufacturing, as well as by mechanical engineering. Analytical technique of pyrolysis chromatography with mass spectroscopy detection was employed to identify organic species in the PM10 fraction. Two datasets (465 determined organic compounds and 50 selected organic markers) were used and compared by multivariate analysis - principal component analysis followed with hierarchical clustering on principal components incorporating compositional data approach. Three resulting clusters were observed in both cases. The cluster representing measurements near plastic processing and manufacturing plants was identical in both the analysed datasets with the same organic compounds that characterized resulting cluster Consequently, leading markers for plastic processing and manufacturing sources were suggested (bumetrizole, bis(tridecyl)phthalate, mono(2-ethylhexyl)phthalate). Other two clusters varied among the analysed datasets, however, dataset with selected markers showed more reliable outcomes. The results imply that concept of using only selected organic marker species with the compositional approach in multivariate statistical methods is sufficient and allows properly distinguishing the main air pollution sources between sampling locations even at a small urban scale.

Comparison of organic compounds in char and soot from the combustion of biomass in boilers of various emission classes.

The combustion of biomass in boilers of emission classes 2 and 3 produces deposits in the form of char and soot inside the combustion chamber. Char and soot differ in content of elemental carbon (EC) and organic carbon (OC) as well as in the content of organic compounds. Deposits from boilers of emission class 2 contain higher amounts of OC and EC than those from boilers of emission class 3. The only exception is deposits formed by the combustion of briquettes from hardwood in boilers of emission class 3 that contained approximately by up to 60 percent higher amount of OC and by approx. 100% more EC than deposits from combustion in boilers of emission class 2. Deposits identified as char are characterized by dominant organic compounds derived from thermic degradation of cellulose, lignin, phytosterols, terpenes, their alteration products, and aromatic hydrocarbons. Deposits identified as soot have dominant PAHs, compounds containing oxygen (furans, benzofurans, phenols) and compounds containing aliphatic nitrogen (benzonitrile). Char from boilers of emission class 2 contains approx. by 80% more alkanes and cycloalkanes, by 80% more nitriles, by 50% more carboxyl acids, by 230% more anhydrosaccharides, phytosterols and by 180% more PAHs. These differences can be utilized for identification of burned fuel.

Water-soluble ions in dust particles depending on meteorological conditions in urban environment.

The particle size distribution of particulate matter (PM) and concentrations of water-soluble ions were observed in airborne aerosol in the City of Ostrava (the Czech Republic, Moravian-Silesian Region) during winter 2016. The Ostrava area ranks among the most polluted areas not only within the Czech Republic but also in Europe due to the high concentration of industry, especially metallurgy, in this area. The selected water-soluble ions: were studied in different grain size classes of particulate matter separated into 7 fractions (from 0.0175 to 9.93 µm) collected by an electrical low pressure impactor ELPI+. Assessment of the influence of meteorological parameters and various sources of contamination (transportation, industry, and residential burning) in heavily environmentally-burdened urban areas was attempted. This study proved that (K)+ is related to metallurgical processes and its role as an indicator of biomass combustion is negligible. The highest concentrations of all water-soluble ions were determined in particles below 0.952 µm that is generally related to combustion processes. At increased air temperature (in the interval from 0 °C to 10 °C), concentrations of both dust particles and the sum of water-soluble ions are decreasing.

Experimental analysis of waste polyurethane from household appliances and its utilization possibilities.

Polyurethane has a good insulation characteristic, and it is widely used as an insulation and lining material for refrigerators. Nevertheless, at the end of a product's lifetime, serious problems arise related to waste management. Recently, energy recovery has been marked as a promising solution, especially, waste-to-energy applications. To find an appropriate application for such waste, a thermal analysis was performed. An experimental analysis of polyurethane (PU) waste material was performed by the method of pyrolysis gas chromatography with mass spectrometric detection (Py-GC/MS) at various pyrolytic temperatures, namely, at 500, 600 and 700 °C. Waste polyurethane foam was conducted to investigations in the form of a bulk sample and sorted grain-size samples with a goal to detect the chemical composition of the pyrolysate. The investigation revealed various groups of organic compounds such as heterocyclic compounds of nitrogen and simple and polycyclic aromatic hydrocarbons, while notable concentrations of compounds containing chlorine were detected as well. The experimental analysis found differences in the composition of amines and other compounds and in the dependence on grain size composition. Bulk samples produced the highest concentration of amines (˃ 40%) at a temperature of 500 °C. The sample homogenization led to a significant increase in amines production. Polyurethane waste of grain size from 0.125 to 0.25 mm contained approximately 80% amines. The pyrolysis of the bulk sample at 600 °C yielded only 24% nitrogen heterocyclic compounds, while from the sorted grain-size <0.045 mm and 0.045-0.063 mm, yields were increased to 47.4 and 45.7%, respectively.

Properties of composts from household food waste produced in automatic composters.

The minimization of landfill deposition of waste containing biological components represents a big problem, especially in built-up residential areas. The main problem of biologically degradable municipal waste is the quantity and composition of household waste which can have an important influence on decomposition processes already in collecting vessels. An amount of household waste produced in residential areas in the Czech Republic varies from 31 to 337 g/person/day, with an average value of 250 g. Waste of plant material character is produced in the range from 22 to 291 g/person/day. Composts were prepared in the home composters from household food waste. After the end of the composting process, compost does not have the organic component sufficiently stabilized, which is documented by a high value of electrical conductivity of aqueous leachate reaching more than 4 mS/cm and low humification index. The value of the humification index pronouncedly increased after eight weeks from 0.05 to 0.85, while compost from composting of green waste using windrow system have humification index of 3.48. An aim of this work is the determination of properties of composts prepared from HFW in automatic composters and identification of differences compared with composts prepared from a mixture of biodegradable green waste.

The possibilities to identify combusted fuel based on an analysis of ash from local heating.

Combustion of undesirable components with a high calorific value (waste: plastics, rubber, chipboard, plywood etc.) contribute to an increased emissions of PM particles. The possibility of identification of non-desirable fuels was verified by analysis of unburned carbon from bottom ash by means of pyrolysis-GC/MS. Compounds derived from thermal conversion of main wood components in the unburned carbon are formed by aldehydes, ketones, alkanes, alkenes, alkanoates, polycyclic aromatic hydrocarbons, compounds containing benzene, compounds containing phenol and nitrogen. Unburned carbon from plywood/chipboard contains compounds with nitrogen (heterocyclic and aliphatic) in increased concentrations (33-51 ng/mg) compared with unburned carbon from pure wood (7-12 ng/mg). The concentrations increased almost two times were proved for compounds containing phenols in unburned carbon from wood composite. Total amount of determined organic compounds is also almost two times higher than that contained in unburned carbon from wood. The indication of waste wood combustion from unburned carbon is possible using the ratio: phenol/(2-methylphenol+4-methylphenol).

Possibilities of the utilization of char from the pyrolysis of tetrapak.

Since the cellulose used in the production of tetrapak is of very high quality, the char generated during pyrolysis should be influenced mainly by the pyrolysis temperature. This article aims to determine the chemical composition of biochar prepared at the temperatures of 400, 500, 600 and 700 °C and its environmental properties determined by the presence of organic compounds with toxicity and relatively high mobility in the environment. The analytical pyrolysis of char was used to identify the following groups of organic compounds: alkanes, cycloalkanes, alkenes, cycloalkenes, alkynes, alkadiens, ethers, alcohols, nitrogen compounds, nitrils, ketones and aldehydes, compounds containing phenols, furans, benzofurans, PAHs (polycyclic aromatic hydrocarbons), carboxylic acids, compounds containing benzenes and markers indicative of the presence of synthetic polymers (polyethylene layers, a part of dyes, antioxidants, stabilizers), and fragments of cellulose. Concerning the use of char as a soil conditioner, its ecotoxicity was monitored (Folsomia candida) by monitoring its addition to the artificial soil (char addition: 0.5, 1, 2.5, 5, 10, 15, 20, 50 and 100%). The lowest reproduction inhibition of Folsomia candida is caused by biochar prepared at the temperature of 400 °C and 700 °C, but it is not suitable for the agricultural application, the concentration of PAHs is three times higher than the EBC limit. Low-density polyethylene which is present in the aseptic box in concentration of 6%, can degrade biochar so that it cannot be used as a soil amendment. The results of the char analyses show that the pyrolysis temperature is a decisive factor in the applicability of biochar.

Environmental impact assessment of the coal yard and ambient pollution.

This study investigates the vertical distribution of pollutants emitted from coal yards using unmanned aerial vehicles (UAVs). Vertical concentration measurements of black carbon (BC) and particulate matter (PM) in a range of 1 m to 100 m above ground level (AGL) in the central coal yard showed clear spatial patterns and gradients of these pollutants. In addition, measurements were taken at specific heights (1 m, 30 m AGL, and 60 m AGL) at seven locations approximately 3 km from the yard. Thirteen measurements were carried out during the non-heating period under similar weather conditions. The measured BC concentrations decreased significantly with increasing altitude, with ground-level concentrations reaching 1.88 ± 0.61 µg/m3 and decreasing by over 46% at 80 m AGL. Similarly, PM10 concentrations at 60 m AGL decreased by 21.7%, with values of 25.99 ± 9.24 µg/m3 measured near the ground level and 16.52 ± 8.31 µg/m3 at 60 m AGL. The maximum coal particle pollution from the coal depot ranges from 500 to 1,000 m. The study showed a significant decrease in BC concentrations with height above the coal yard surface. Concentrations of PM10 and PM10-TSP showed a complex distribution influenced by local emissions and long-range particle transport. Meteorological factors, especially wind speed and direction, significantly influenced the pollutant dispersion. In addition, higher pollutant concentrations were measured during dry periods than after rainfall. The findings of this study contribute to a better understanding of the dispersion patterns and potential impacts of coal dust, enabling the implementation of targeted mitigation strategies and improved pollution control measures.

Plastic particles in urban compost and their grain size distribution.

Gathering information on plastic particles in composts and the processes they undergo is important in terms of potentially limiting their further entry into the environment, for example, in improving the fertilising properties of soils. Microplastics (MPs) were determined in composts produced from urban greenery. They are present in decreasing order: polyethylene terephthalate, polystyrene, polyethylene, and polypropylene. The determination of polymers and additives used to improve their properties was performed by pyrolysis and gas chromatography with mass spectrometric detection (Py-GC/MS). Additives and microplastics are most concentrated in composts in the 0.315-0.63 and 0.63-1.25 mm grain size class, together with the carbon contained in the compost dry matter. Additives form 0.11-0.13% of MPs in dry matter of compost. The average concentration of microplastics in the particle size class from 0.63 to 1.25 mm is 2434 ± 224 mg/kg; in the total sample of composts, it is 1368 ± 286 mg/kg of P-MPs. For composts with particle size <2.5 mm, a relationship between the C/N ratio and the plastic particle concentration was statistically significant. It documents a similar behaviour of lignocellulose and plastic particles during the degradation processes. A relationship between the concentration of polymer markers and additives in the compost dry matter and their concentrations in the leachate has been demonstrated. The leachability from compost is higher for additives than for chemical compounds originating from the decomposition of the main components of MPs. The suitability of the use of the compost for agricultural purposes was monitored by the germination index (GI) for watercress. The lowest value of the GI was determined in the particle size class from 0.63 to 1.25 mm. The leachability of polymer markers and additives alone cannot explain the low GI value in this grain size class. The GI value is also influenced by the leachability of chemical compounds characterised by the value of dissolved organic carbon (DOC) and water-leachable nitrogen (Nw). A statistically significant dependence between DOC/Nw and the germination index value was found.

Effect of biochar addition on the improvement of the quality parameters of compost used for land reclamation.

The 5% addition of biochar in composting in rows contributes significantly to reducing volatile organic compound(VOC) emissions. When composting with the addition of biochar, the average temperature increased by 13 ± 6.7 °C during the whole period, and the thermophilic phase was extended by 11 days. The higher temperature supported a reduction in the time necessary for achieving the biological stability observed by the oxygen uptake rate by more than 10 days. For organic compounds formed by the degradation of easily degradable primary components (proteins), the addition of biochar significantly reduces the release of organic compounds with heterocyclically bound nitrogen (Norg-VOCs) and volatile sulfur compounds (VSCs). The end of the biodegradation process is indicated by a decrease in VOC concentrations below initial values in the input material. This state was achieved in the compost with added biochar after 47 days, while in compost without added biochar, it lasted 60 days.

The use of polymer compounds in the deposits from the combustion of briquettes in domestic heating as an identifier of fuel quality.

The utilisation of waste wood from furniture production brings new problems connected with an incomplete thermochemical decomposition of additives (chemicals for improving properties of plastics) in small heating with the addition of sources. Unique organic compounds produced by the combustion of waste wood allow the identification of the type of fuel. The organic compounds contained in the char deposits were analysed by pyrolysis gas chromatography with mass spectrometry. The deposits from the combustion of briquettes from furniture production contain organic compounds originating by decomposition of phenolic resins, aminoplasts (urea-formaldehyde, resorcinol-formaldehyde and melamine), polyurethanes and wood glue. Additives contained in the deposits include plasticisers such as phthalates (DEHP, dibutyl phthalate and diisobutyl phthalate), flame retardants (2-propanol, 1-chlorophosphate (3:1) and p-terphenyl). Deposits from the combustion of briquettes from virgin wood do not contain these compounds. The total amount of compounds identified in the deposits from the boiler, which do not come from virgin wood combustion, varies in the range between 4.25 and 6.25 g/kg. Phthalates (55.5%) and PVAc adhesives (18.6%) are the main anthropogenic compounds in the deposits from domestic boilers.

Assembly of the cnidarian camera-type eye from vertebrate-like components.

Animal eyes are morphologically diverse. Their assembly, however, always relies on the same basic principle, i.e., photoreceptors located in the vicinity of dark shielding pigment. Cnidaria as the likely sister group to the Bilateria are the earliest branching phylum with a well developed visual system. Here, we show that camera-type eyes of the cubozoan jellyfish, Tripedalia cystophora, use genetic building blocks typical of vertebrate eyes, namely, a ciliary phototransduction cascade and melanogenic pathway. Our findings indicative of parallelism provide an insight into eye evolution. Combined, the available data favor the possibility that vertebrate and cubozoan eyes arose by independent recruitment of orthologous genes during evolution.

Recording fine-scale movement of ground beetles by two methods: Potentials and methodological pitfalls.

Movement trajectories are usually recorded as a sequence of discrete movement events described by two parameters: step length (distance) and turning angle (bearing). One of the most widespread methods to record the geocoordinates of each step is by a GPS device. Such devices have limited suitability for recording fine movements of species with low dispersal ability including flightless carabid beetles at small spatio-temporal scales. As an alternative, the distance-bearing approach can avoid the measurement error of GPS units since it uses directly measured distances and compass azimuths. As no quantification of measurement error between distance-bearing and GPS approaches exists so far, we generated artificial fine-scale trajectories and in addition radio-tracked living carabids in a temperate forest and recorded each movement step by both methods. Trajectories obtained from distance-bearing were compared to those obtained by a GPS device in terms of movement parameters. Consequently, both types of trajectories were segmented by state-switching modeling into two distinct movement stages typical for carabids: random walk and directed movement. We found that the measurement error of GPS compared to distance-bearing was 1.878 m (SEM = 0.181 m) for distances and 31.330° (SEM = 2.066°) for bearings. Moreover, these errors increased under dense forest canopy and rainy weather. Distance error did not change with increasing distance recorded by distance-bearing but bearings were significantly more sensitive to error at short distances. State-switching models showed only slight, not significant, differences in movement states between the two methods in favor of the random walk in the distance-bearing approach. However, the shape of the GPS-measured trajectories considerably differed from those recorded by distance-bearing caused especially by bearing error at short distances. Our study showed that distance-bearing could be more appropriate for recording movement steps not only of ground-dwelling beetles but also other small animals at fine spatio-temporal scales.

The quality of composts prepared in automatic composters from fruit waste generated by the production of beverages.

Ensuring the processing of food waste from the production of food and beverages intheautomatic composters can be difficult because of the physicochemical properties of input raw materials. Very often, the final product does not meet the requirements forcomposts according to the European Compost Network. Optimisation of input food waste from theproduction ofbeverages was performed by the addition of the bulk materials such assawdust and clay minerals (bentonite). Toxicity of the compost is caused by organic compounds with polar and non-polar properties. These compounds belong to the groups ofalcohols, aldehydes and ketones, carboxylic acids, tannin, and phenols, coumarins and terpenes. Phytotoxicity is mostly influenced by the group of terpenes. The addition ofsawdust used as bulking agent decreases the concentrations of almost all chemical compounds. Thegroup of tannin and compounds containing phenols represents an exception because these compounds are released from sawdust.

Environmental risks related to organic compounds from the combustion of paper briquettes in domestic boilers.

Environmental risks connected with the combustion of paper/cardboard briquettes are still not sufficiently known. This paper aims to bring attention to the risks related to the utilisation of paper briquettes in local boilers and to characterise these risks by means of the identification of organic compounds in deposits from exhaust flues. The identification of the chemical compounds was performed by pyrolysis gas chromatography with mass spectrometric detection. Paper/cardboard briquettes contain 119 compounds of biogenic origin derived from major biomass components and 53 additives. Additives are used both for improving the properties of paper and in printing inks. By burning the paper briquettes, the same 53 compounds from the additive group were caught in the deposits from the flue gas pathway, occurring in the range of 1-10% of the concentration of individual compounds (additives) contained in the input fuel. Compounds that are very stable during the combustion process have an enrichment factor (EF) >30, which corresponded to approximately 3% of the additive capture in deposits. The highest values were found for plasticisers (phthalates). Many of the primary organic compounds contained in the input raw material do not decompose during combustion and can have adverse effects on human health.

The potential environmental risks of the utilization of composts from household food waste.

Modern technologies (especially with the help of autonomous measurement and control systems) introduced automatic composters for the disposal of household food waste production. Environmental risks connected with the utilization of these composts can be characterized by the high electrical conductivity caused by a presence of sodium chloride in food. Electrical conductivity influences the ecotoxicity of the composts. The presence of pesticides in composted food also represents an important environmental problem. The following pesticides were found in compost samples from household food waste: 1,3,5-triazine, methyl trithion, bifenthrin, bifenox, carbophenothion, pirimicarb, dioxacarb, desmetryn. Pesticide content in composts varied from 0.3 to 16.3 µg/kg, the average value being 30.4 ± 10.1 µg/kg dry matter. The higher decomposition was found of "modern" pesticides in the composters. The removal of salts can ensure that inhibition will be < 30% while washing with the ratio of 1:3 will result in the inhibition < 5%. However, this way of processing is not effective for other organisms-Poecilia reticulata (mortality 100%) and Daphnia magna (immobilisation 100%) using this procedure as well as washing of the compost in the ratio 3:1.

Lens morphogenesis is dependent on Pax6-mediated inhibition of the canonical Wnt/beta-catenin signaling in the lens surface ectoderm.

Lens formation in mouse is critically dependent on proper development of the retinal neuroectoderm that is located close beneath the head surface ectoderm. Signaling from the prospective retina triggers lens-specific gene expression in the surface-ectoderm. Supression of canonical Wnt/beta-catenin signaling in the surface ectoderm is one of the prerequisites for lens development because, as we show here, ectopic Wnt activation in the retina and lens abrogates lens formation. Wnt inhibiton is mediated by signals coming from the retina but its exact mechanism is unknown. We show that Pax6 directly controls expression of several Wnt inhibitors such as Sfrp1, Sfrp2, and Dkk1 in the presumptive lens. In accordance, absence of Pax6 function leads to aberrant canonical Wnt activity in the presumptive lens that subsequently impairs lens development. Thus Pax6 is required for down-regulation of canonical Wnt signaling in the presumptive lens ectoderm.