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1.
Trends Genet ; 38(4): 307-309, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35135699

RESUMO

Modern wheat shows phenomenal evolutional success and adaptability to a range of environments owing to polyploidization; however, during its hybridization process a major genetic gain has been overlooked. Recently, Gaurav et al. emphasized harnessing genetic diversity from wheat wild progenitor Aegilops tauschii for the improvement of hexaploid wheat through introgression or transgenesis.


Assuntos
Aegilops , Aegilops/genética , Triticum/genética
2.
Mol Biol Rep ; 51(1): 537, 2024 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-38642174

RESUMO

BACKGROUND: Hexaploid bread wheat underwent a series of polyploidization events through interspecific hybridizations that conferred adaptive plasticity and resulted in duplication and neofunctionalization of major agronomic genes. The genetic architecture of polyploid wheat not only confers adaptive plasticity but also offers huge genetic diversity. However, the contribution of different gene copies (homeologs) encoded from different subgenomes (A, B, D) at different growth stages remained unexplored. METHODS: In this study, hybrid of elite cultivars of wheat were developed via reciprocal crosses (cytoplasm swapping) and phenotypically evaluated. We assessed differential expression profiles of yield-related negative regulators in these cultivars and their F1 hybrids and identified various cis-regulatory signatures by employing bioinformatics tools. Furthermore, the preferential expression patterns of the syntenic triads encoded from A, B, and D subgenomes were assessed to decipher their functional redundancy at six different growth stages. RESULTS: Hybrid progenies showed better heterosis such as up to 17% increase in the average number of grains and up to 50% increase in average thousand grains weight as compared to mid-parents. Based on the expression profiling, our results indicated significant dynamic transcriptional expression patterns, portraying the different homeolog-dominance at the same stage in the different cultivars and their hybrids. Albeit belonging to same syntenic triads, a dynamic trend was observed in the regulatory signatures of these genes that might be influencing their expression profiles. CONCLUSION: These findings can substantially contribute and provide insights for the selective introduction of better cultivars into traditional and hybrid breeding programs which can be harnessed for the improvement of future wheat.


Assuntos
Melhoramento Vegetal , Triticum , Triticum/genética , Hibridização Genética , Vigor Híbrido/genética
3.
Tidsskr Nor Laegeforen ; 144(7)2024 Jun 04.
Artigo em Inglês, Norueguês | MEDLINE | ID: mdl-38832610

RESUMO

Background: While most cases of venous thromboembolism follow a benign course, occasionally the condition may manifest a complex clinical presentation and need a comprehensive diagnostic workup to identify the underlying cause and provide the patient with appropriate treatment. Case presentation: A woman in her late thirties presented to the emergency department with a five-day history of dyspnoea. She had recently undergone liposuction surgery after pregnancy. Upon admission, initial investigations revealed a pulmonary embolism with right heart strain, and she was treated with anticoagulants. The following day, she complained of acute-onset right flank pain without fever or other accompanying symptoms. A CT scan of the abdomen confirmed a right-side renal infarction. Further investigations revealed patent foramen ovale between the right and left atria of the heart, believed to be the source of a right-to-left shunt of arterial emboli. Although the patient had not suffered a clinical stroke, it was decided to close this defect using percutaneous technique. Interpretation: Patent foramen ovale is a common condition in adults, but in most cases it remains asymptomatic. However, patients with patent foramen ovale have an elevated risk of arterial emboli affecting multiple organs. The diagnosis depends on thorough assessment to prevent potentially fatal outcomes.


Assuntos
Abdominoplastia , Dispneia , Forame Oval Patente , Embolia Pulmonar , Humanos , Feminino , Adulto , Forame Oval Patente/complicações , Forame Oval Patente/cirurgia , Forame Oval Patente/diagnóstico por imagem , Dispneia/etiologia , Abdominoplastia/efeitos adversos , Embolia Pulmonar/etiologia , Embolia Pulmonar/diagnóstico por imagem , Tomografia Computadorizada por Raios X , Infarto/etiologia , Infarto/diagnóstico por imagem , Infarto/diagnóstico , Infarto/cirurgia , Complicações Pós-Operatórias
4.
Cardiology ; 148(3): 219-227, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36948161

RESUMO

BACKGROUND: ECG is the initial diagnostic tool that in combination with typical symptoms often raises the suspicion of pericarditis. Echocardiography remains the first-line imaging modality for assessment of pericardial diseases, particularly effusion/tamponade, constrictive physiology, and assessment of regional wall motion abnormalities as differential diagnoses. However, cardiac CT and cardiac magnetic resonance may be necessary in complicated cases and to identify pericardial inflammation in specific settings (atypical presentation, new onset constriction), as well as myocardial involvement and monitoring the disease activity. SUMMARY: In acute pericarditis, the most commonly used ECG criteria recommended by international guidelines are the widespread ST-segment elevation or PR depression. However, the classic ECG pattern of widespread ST-segment elevation or PR depression can be seen in less than 60% of patients. In addition, ECG changes are often temporally dynamic, evolve rapidly during the course of disease, and may be influenced by a number of factors such as disease severity, time (stage) of presentation, degree of myocardial involvement, and the treatment initiated. Overall, temporal dynamic changes on ECG during acute pericarditis or myopericarditis have received limited attention. Hence, the aim of this brief clinical review was to increase awareness about the various ECG changes observed during the course of acute pericarditis. KEY MESSAGES: ECG may be normal at presentation or for days after the index episode of chest pain, but serial ECGs can reveal specific patterns of temporally dynamic ST elevation in patients with pericarditis or myopericarditis, particularly during new episodes of chest pain.


Assuntos
Miocardite , Pericardite , Humanos , Doença Aguda , Arritmias Cardíacas/complicações , Dor no Peito/etiologia , Ecocardiografia , Eletrocardiografia , Miocardite/diagnóstico por imagem , Pericardite/diagnóstico por imagem
5.
J Appl Microbiol ; 133(5): 3094-3112, 2022 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-35908279

RESUMO

AIMS: Soil microbial communities are among the most diverse communities that might be affected due to transgenic crops. Therefore, risk assessment studies on transgenes are essentially required as any adverse effects may depend not only on the specific gene and crop involved but also on soil conditions. METHODS AND RESULTS: The present study deals with the comparison of bacterial populations, root exudates and activities of soil enzymes in nontransgenic and AVP1-transgenic wheat rhizosphere, overexpressing vacuolar H + pyrophosphatase for salinity and drought stress tolerance. Amounts of organic acids and sugars produced as root exudates and activities of dehydrogenase, phosphatase and protease enzymes in soil solution showed no significant differences in AVP1-transgenic and nontransgenic wheat rhizosphere, except for urease and phenol oxidase activities. The higher copy number of nifH gene showed the abundance of nitrogen-fixing bacteria in the rhizosphere of AVP1-transgenic wheat compared with nontransgenic wheat. nifH gene sequence analysis indicated the common diazotrophic genera Azospirillum, Bradyrhizobium, Rhizobium and Pseudomonas in AVP1-transgenic and nontransgenic wheat except for Zoogloea detected only in nontransgenic wheat. Using 454-pyrosequencing of 16S rRNA gene from soil DNA, a total of 156, 282 sequences of 18 phyla were obtained, which represented bacterial (128,006), Archeal (7928) and unclassified (21,568) sequences. Proteobacteria, Crenarchaeota and Firmicutes were the most abundant phyla in the transgenic and nontransgenic wheat rhizosphere. Further comparison of different taxonomic units at the genus level showed similar distribution in transgenic and nontransgenic wheat rhizospheres. CONCLUSION: We conclude that the AVP1 gene in transgenic wheat has no apparent adverse effects on the soil environment and different bacterial communities. However, the bacterial community depends on several other factors, not only genetic composition of the host plants. SIGNIFICANCE OF THE STUDY: The present research supports introduction and cultivation of transgenic plants in agricultural systems without any adverse effects on indigenous bacterial communities and soil ecosystems.


Assuntos
Microbiota , Rizosfera , Triticum/microbiologia , Solo , RNA Ribossômico 16S/genética , Microbiologia do Solo , Urease , Monofenol Mono-Oxigenase , Bactérias/genética , Microbiota/genética , Exsudatos e Transudatos , Açúcares , Monoéster Fosfórico Hidrolases , Peptídeo Hidrolases , Pirofosfatases
6.
Sensors (Basel) ; 22(2)2022 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-35062432

RESUMO

Fifth-generation and beyond networks target multiple distributed network application such as Internet of Things (IoT), connected robotics, and massive Machine Type Communication (mMTC). In the absence of a central management unit, the device need to search and establish a route towards the destination before initializing data transmission. In this paper, we proposes a destination search and routing method for distributed 5G and beyond networks. In the proposed method, the source node makes multiple attempts to search for a route towards the destination by expanding disk-like patterns originating from the source node. The source node increases the search area in each attempt, accommodating more nodes in the search process. As a result, the probability of finding the destination increases, which reduces energy consumption and time delay of routing. We propose three variants of routing for high, medium, and low-density network scenarios and analyze their performance for various network configurations. The results demonstrate that the performance of the proposed solution is better than previously proposed techniques in terms of time latency and reduced energy consumption, making it applicable for 5G and beyond networks.

7.
Sensors (Basel) ; 20(17)2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32825318

RESUMO

Localization is an indispensable technology for underwater wireless sensor networks (UWSNs). In what concerns UWSNs, the accurate location information is not only the requirement of the marine field applications but also the basis of the other corresponding research, for instance, network routing and topology control. Recently, an astonishing surge of interest has been drawn in the received signal strength (RSS)-based scheme due to cost-effectiveness and synchronization-free compared with others. However, unlike the terrestrial wireless sensor networks (WSNs), the acoustic signal may suffer the absorption loss in the underwater environment besides the path loss, which degrades the localization accuracy and limits the capability of the RSS-based technology in UWSNs. In this context, a robust localization method with an absorption mitigation technique (AMT) is developed. First, an RSS-based analytically tractable measurement model is conducted, where the maximum likelihood estimator (MLE) is derived. Nevertheless, it is quite challenging to solve the problem using MLE under a non-convex expression. Therefore, by exploiting certain approximations, the considered localization problem is converted into an optimization expression with a maximum absorption loss involved. A min-max strategy is then presented, with which the problem is turned to minimize the worst situation of the absorption loss. After a simple manipulation, the problem is further investigated as a generalized trust region sub-problem (GTRS) framework. Although the GTRS is a non-convex scheme, the solution can be obtained through an iteration method by introducing a multiplier. In addition, the closed-form expression of the Cramer-Rao lower bound (CRLB) of the analytically tractable measurement model is derived. Numerical simulations demonstrate the effectiveness of the proposed method compared with the state-of-the-art approaches in different scenarios.

8.
Sensors (Basel) ; 19(9)2019 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-31052300

RESUMO

It is crucial to estimate the location of primary users (PUs) for the development of cognitive radio networks (CRNs). Great efforts have been made in the past to develop localization algorithms with better accuracy but low computation. In CRNs, PUs do not cooperate with secondary users (SUs), which makes the localization task challenging. Due to this feature, received signal strength (RSS)-based PU localization techniques, such as centroid localization (CL) and multidimensional scaling (MDS), are the best candidates. However, most of the CL- and MDS-based PU localization methods consider omnidirectional wireless communication. Therefore, in this paper we propose a PU localization method which uses the RSS values at different sectors of the SU antenna, where a scoring strategy is applied to all the sectors to estimate the PU location. Two different scoring functions are proposed. Numerical results show that the proposed localization method is robust to PU locations and channel conditions. The proposed method is validated in terms of various network parameters, such as the number of SUs, beamwidth of the SU sectors, size of the grid, and placement of the PUs. Results show that increasing the number of SUs improve the localization accuracy due to an increased number of measurements. However, the localization accuracy degrades with an increase in the beamwidth of the SU sector because the faraway grid points also participate in the localization. The results are also compared with the conventional CL for PU localization. Compared with conventional CL, it offers a significant improvement in the performance.

9.
Sensors (Basel) ; 18(1)2017 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-29278405

RESUMO

Underwater wireless technologies demand to transmit at higher data rate for ocean exploration. Currently, large coverage is achieved by acoustic sensor networks with low data rate, high cost, high latency, high power consumption, and negative impact on marine mammals. Meanwhile, optical communication for underwater networks has the advantage of the higher data rate albeit for limited communication distances. Moreover, energy consumption is another major problem for underwater sensor networks, due to limited battery power and difficulty in replacing or recharging the battery of a sensor node. The ultimate solution to this problem is to add energy harvesting capability to the acoustic-optical sensor nodes. Localization of underwater sensor networks is of utmost importance because the data collected from underwater sensor nodes is useful only if the location of the nodes is known. Therefore, a novel localization technique for energy harvesting hybrid acoustic-optical underwater wireless sensor networks (AO-UWSNs) is proposed. AO-UWSN employs optical communication for higher data rate at a short transmission distance and employs acoustic communication for low data rate and long transmission distance. A hybrid received signal strength (RSS) based localization technique is proposed to localize the nodes in AO-UWSNs. The proposed technique combines the noisy RSS based measurements from acoustic communication and optical communication and estimates the final locations of acoustic-optical sensor nodes. A weighted multiple observations paradigm is proposed for hybrid estimated distances to suppress the noisy observations and give more importance to the accurate observations. Furthermore, the closed form solution for Cramer-Rao lower bound (CRLB) is derived for localization accuracy of the proposed technique.

10.
Sensors (Basel) ; 16(9)2016 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-27618061

RESUMO

We propose a novel cluster based cooperative spectrum sensing algorithm to save the wastage of energy, in which clusters are formed using fuzzy c-means (FCM) clustering and a cluster head (CH) is selected based on a sensor's location within each cluster, its location with respect to fusion center (FC), its signal-to-noise ratio (SNR) and its residual energy. The sensing information of a single sensor is not reliable enough due to shadowing and fading. To overcome these issues, cooperative spectrum sensing schemes were proposed to take advantage of spatial diversity. For cooperative spectrum sensing, all sensors sense the spectrum and report the sensed energy to FC for the final decision. However, it increases the energy consumption of the network when a large number of sensors need to cooperate; in addition to that, the efficiency of the network is also reduced. The proposed algorithm makes the cluster and selects the CHs such that very little amount of network energy is consumed and the highest efficiency of the network is achieved. Using the proposed algorithm maximum probability of detection under an imperfect channel is accomplished with minimum energy consumption as compared to conventional clustering schemes.

11.
Mol Biol Rep ; 41(3): 1669-82, 2014 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-24420850

RESUMO

Abiotic stresses such as salinity and drought have adverse effects on plants. In the present study, a Na(+)/H(+) antiporter gene homologue (LfNHX1) has been cloned from a local halophyte grass (Leptochloa fusca). The LfNHX1 cDNA contains an open reading frame of 1,623 bp that encodes a polypeptide chain of 540 amino acid residues. LfNHX1 protein sequence showed high similarity with NHX1 homologs reported from other halophyte plants. Amino acid and nucleotide sequence similarity, protein topology modeling and the presence of conserved functional domains in the LfNHX1 protein sequence classified it as a vacuolar NHX1 homolog. The overexpression of LfNHX1 gene under CaMV35S promoter conferred salt and drought tolerance in tobacco plants. Under drought stress, transgenic plants showed higher relative water contents, photosynthetic rate, stomatal conductance and membrane stability index as compared to wild type plants. More negative value of leaf osmotic potential was also observed in transgenic plants when compared with wild type control plants. Transgenic plants showed better germination and root growth at 2 mg L(-1) Basta herbicide and three levels (100, 200 and 250 mM) of sodium chloride. These results showed that LfNHX1 is a potential candidate gene for enhancing drought and salt tolerance in crops.


Assuntos
Proteínas de Arabidopsis/genética , Proteínas de Transporte de Cátions/genética , Secas , Poaceae/genética , Tolerância ao Sal/genética , Trocadores de Sódio-Hidrogênio/genética , Arabidopsis/genética , Clonagem Molecular , Regulação da Expressão Gênica de Plantas , Germinação/genética , Fotossíntese/genética , Folhas de Planta/genética , Proteínas de Plantas/biossíntese , Plantas Geneticamente Modificadas/genética , Salinidade , Tolerância ao Sal/fisiologia
12.
Curr Probl Cardiol ; 49(1 Pt C): 102158, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-37865301

RESUMO

Whereas the electrocardiogram (ECG) changes in hypokalemia are well known, they often receive less attention than the more striking features of hyperkalemia. Furthermore, there is a need for further discussion as to the subtleties of ECG changes that can aid in the differential diagnoses. This case study presents the ECG changes of a patient with severe hypokalemia due to diarrhea. It highlights how bifid T-waves in hypokalemia can be distinguished from other conditions such as coronary artery disease or pericarditis. Furthermore, it also shows the gradual reversal of ECG changes in the same patient when potassium is normalized.


Assuntos
Hiperpotassemia , Hipopotassemia , Humanos , Hipopotassemia/diagnóstico , Hipopotassemia/etiologia , Eletrocardiografia , Potássio , Arritmias Cardíacas/diagnóstico , Arritmias Cardíacas/etiologia , Hiperpotassemia/diagnóstico , Hiperpotassemia/etiologia , Hiperpotassemia/terapia
13.
Pak J Med Sci ; 29(4): 1055-8, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24353687

RESUMO

OBJECTIVE: To assess the effectiveness of sodium hyaluronate Eye Gel in Dry Eye Disease (DED) patients Methods: A Multi-center, Open-label, Uncontrolled clinical trial was conducted in different centers of Pakistan. Ten ophthalmologists conducted this study in which 250 diagnosed patients with dry eye disease were enrolled after obtaining a written informed consent. Ten patients were dropped out during the study period. All patients were assessed as per following criteria for enrolling a patient into the study: Tear Film Break - up time (TBUT) ≤ 10 seconds, Schirmer Test - 1 ≤ 6 mm / 5 minutes and Positive Corneal Staining. Tolerability/safety was assessed by the monitoring and recording of all adverse events. The physical examination was done at baseline, 4(th) week and 8(th) week. Results : The mean age of the patients was 43.4 ±17.8 years and out of 240 patients 117 (48.7%) were males and 123 (51.3%) females. The Mean duration of symptoms was 19.3±23.9. At the initial visit the foreign body sensation was 50.6%, itching 35.9%, burning 50.6%, watering 42.9%, photophobia 25.3% and feeling of dryness in 14.7% of patients. After 4 weeks (2(nd) visit), the symptoms were decreased to 47.1% foreign body sensation, 32.4% itching, 48.2% burning, 41.8% watering, 25.3% photophobia and 13.5% feeling of dryness. At the 3(rd) visit (after 8 weeks) the frequency of symptoms were: 45.3% foreign body sensation, 30.6% itching, 45.9% burning, 40% watering, 24.7% photophobia and 13.5% feeling of dryness. CONCLUSION: Sodium Hyaluronate can provide a suitable alternate in the treatment of dry eye disease due to its reported efficacy on foreign body sensation, itching, burning, watering, photophobia and feeling of dryness.

14.
Front Plant Sci ; 14: 1076331, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36760629

RESUMO

Plant's perception of heat stress involves several pathways and signaling molecules, such as phosphoinositide, which is derived from structural membrane lipids phosphatidylinositol. Phospholipase C (PLC) is a well-known signaling enzyme containing many isoforms in different organisms. In the present study, Phospholipase C Isoform 5 (PLC5) was investigated for its role in thermotolerance in Arabidopsis thaliana. Two over-expressing lines and one knock-down mutant of PLC5 were first treated at a moderate temperature (37 °C) and left for recovery. Then again exposed to a high temperature (45 °C) to check the seedling viability and chlorophyll contents. Root behavior and changes in 32Pi labeled phospholipids were investigated after their exposure to high temperatures. Over-expression of PLC5 (PLC5 OE) exhibited quick and better phenotypic recovery with bigger and greener leaves followed by chlorophyll contents as compared to wild-type (Col-0) and PLC5 knock-down mutant in which seedling recovery was compromised. PLC5 knock-down mutant illustrated well-developed root architecture under controlled conditions but stunted secondary roots under heat stress as compared to over-expressing PLC5 lines. Around 2.3-fold increase in phosphatidylinositol 4,5-bisphosphate level was observed in PLC5 OE lines upon heat stress compared to wild-type and PLC5 knock-down mutant lines. A significant increase in phosphatidylglycerol was also observed in PLC5 OE lines as compared to Col-0 and PLC5 knock-down mutant lines. The results of the present study demonstrated that PLC5 over-expression contributes to heat stress tolerance while maintaining its photosynthetic activity and is also observed to be associated with primary and secondary root growth in Arabidopsis thaliana.

15.
Front Plant Sci ; 14: 1127311, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37008481

RESUMO

Globally, wheat is the major source of staple food, protein, and basic calories for most of the human population. Strategies must be adopted for sustainable wheat crop production to fill the ever-increasing food demand. Salinity is one of the major abiotic stresses involved in plant growth retardation and grain yield reduction. In plants, calcineurin-B-like proteins form a complicated network with the target kinase CBL-interacting protein kinases (CIPKs) in response to intracellular calcium signaling as a consequence of abiotic stresses. The AtCIPK16 gene has been identified in Arabidopsis thaliana and found to be significantly upregulated under salinity stress. In this study, the AtCIPK16 gene was cloned in two different plant expression vectors, i.e., pTOOL37 having a UBI1 promoter and pMDC32 having a 2XCaMV35S constitutive promoter transformed through the Agrobacterium-mediated transformation protocol, in the local wheat cultivar Faisalabad-2008. Based on their ability to tolerate different levels of salt stress (0, 50, 100, and 200 mM), the transgenic wheat lines OE1, OE2, and OE3 expressing AtCIPK16 under the UBI1 promoter and OE5, OE6, and OE7 expressing the same gene under the 2XCaMV35S promoter performed better at 100 mM of salinity stress as compared with the wild type. The AtCIPK16 overexpressing transgenic wheat lines were further investigated for their K+ retention ability in root tissues by utilizing the microelectrode ion flux estimation technique. It has been demonstrated that after 10 min of 100 mM NaCl application, more K+ ions were retained in the AtCIPK16 overexpressing transgenic wheat lines than in the wild type. Moreover, it could be concluded that AtCIPK16 functions as a positive elicitor in sequestering Na+ ions into the cell vacuole and retaining more cellular K+ under salt stress to maintain ionic homeostasis.

16.
Bioengineering (Basel) ; 10(7)2023 Jul 18.
Artigo em Inglês | MEDLINE | ID: mdl-37508877

RESUMO

Artificial neural networks (ANNs) ability to learn, correct errors, and transform a large amount of raw data into beneficial medical decisions for treatment and care has increased in popularity for enhanced patient safety and quality of care. Therefore, this paper reviews the critical role of ANNs in providing valuable insights for patients' healthcare decisions and efficient disease diagnosis. We study different types of ANNs in the existing literature that advance ANNs' adaptation for complex applications. Specifically, we investigate ANNs' advances for predicting viral, cancer, skin, and COVID-19 diseases. Furthermore, we propose a deep convolutional neural network (CNN) model called ConXNet, based on chest radiography images, to improve the detection accuracy of COVID-19 disease. ConXNet is trained and tested using a chest radiography image dataset obtained from Kaggle, achieving more than 97% accuracy and 98% precision, which is better than other existing state-of-the-art models, such as DeTraC, U-Net, COVID MTNet, and COVID-Net, having 93.1%, 94.10%, 84.76%, and 90% accuracy and 94%, 95%, 85%, and 92% precision, respectively. The results show that the ConXNet model performed significantly well for a relatively large dataset compared with the aforementioned models. Moreover, the ConXNet model reduces the time complexity by using dropout layers and batch normalization techniques. Finally, we highlight future research directions and challenges, such as the complexity of the algorithms, insufficient available data, privacy and security, and integration of biosensing with ANNs. These research directions require considerable attention for improving the scope of ANNs for medical diagnostic and treatment applications.

17.
Big Data ; 10(1): 54-64, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-34788074

RESUMO

The biosensors on a human body form a wireless body area network (WBAN) that can examine various physiological parameters, such as body temperature, electrooculography, electromyography, electroencephalography, and electrocardiography. Deep learning can use health information from the embedded sensors on the human body that can help monitoring diseases and medical disorders, including breathing issues and fever. In the context of communication, the links between the sensors are influenced by fading due to diffraction, reflection, shadowing by the body, clothes, body movement, and the surrounding environment. Hence, the channel between sensors and the central unit (CU), which collects data from sensors, is practically imperfect. Therefore, in this article, we propose a deep learning-based COVID-19 detection scheme using a WBAN setup in the presence of an imperfect channel between the sensors and the CU. Moreover, we also analyze the impact of correlation on WBAN by considering the imperfect channel. Our proposed algorithm shows promising results for real-time monitoring of COVID-19 patients.


Assuntos
COVID-19 , Doenças Transmissíveis , Redes de Comunicação de Computadores , Humanos , SARS-CoV-2 , Tecnologia sem Fio
18.
Front Plant Sci ; 13: 881188, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35774812

RESUMO

The ensuing heat stress drastically affects wheat plant growth and development, consequently compromising its grain yield. There are many thermoregulatory processes/mechanisms mediated by ion channels, lipids, and lipid-modifying enzymes that occur in the plasma membrane and the chloroplast. With the onset of abiotic or biotic stresses, phosphoinositide-specific phospholipase C (PI-PLC), as a signaling enzyme, hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) which is further phosphorylated into phosphatidic acid (PA) as a secondary messenger and is involved in multiple processes. In the current study, a phospholipase C (PLC) signaling pathway was investigated in spring wheat (Triticum aestivum L.) and evaluated its four AtPLC5 overexpressed (OE)/transgenic lines under heat and osmotic stresses through 32Pi radioactive labeling. Naturally, the wheat harbors only a small amount of PIP2. However, with the sudden increase in temperature (40°C), PIP2 levels start to rise within 7.5 min in a time-dependent manner in wild-type (Wt) wheat. While the Phosphatidic acid (PA) level also elevated up to 1.6-fold upon exposing wild-type wheat to heat stress (40°C). However, at the anthesis stage, a significant increase of ∼4.5-folds in PIP2 level was observed within 30 min at 40°C in AtPLC5 over-expressed wheat lines. Significant differences in PIP2 level were observed in Wt and AtPLC5-OE lines when treated with 1200 mM sorbitol solution. It is assumed that the phenomenon might be a result of the activation of PLC/DGK pathways. Together, these results indicate that heat stress and osmotic stress activate several lipid responses in wild-type and transgenic wheat and can explain heat and osmotic stress tolerance in the wheat plant.

19.
Plant Physiol Biochem ; 180: 124-133, 2022 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-35427995

RESUMO

Heterologous expression of plant genes is becoming an important strategy for the improvement of specific traits in existing cultivars. This study presents the response of a salt-sensitive high-yielding wheat variety under stress-inducible expression of barley HVA1 gene belonging to the Late embryogenesis abundance (LEA) gene family. Six homozygous transgenic wheat plants were developed and advanced for testing under various water regimes and salt stress conditions. Putative transgenic plants showed better germination and root shoot development at the early developmental stages under drought stress conditions. Moreover, transgenic plants illustrated higher values of physiological features as compared to non-transgenic plants under both drought and salinity stresses that indicate improved physiological processes in transgenic plants. Higher membrane stability index (MSI) and lower electrolyte leakage (EL) after exposure to abiotic stresses reveal improved cellular membrane stability (CMS) and reduced injury to chloroplast membrane. Interestingly, under salinity stress, transgenic wheat plants showed preference towards higher K+ accumulation in the shoot, which is not a well-understood HVA1 mediated Na + avoidance mechanism under excessive subsurface salts. The predisposition of K+/Na + under salt stress conditions on heterologous expression of the HVA1 gene in wheat needs to be studied in detail in further studies.


Assuntos
Secas , Triticum , Expressão Gênica , Regulação da Expressão Gênica de Plantas , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Estresse Fisiológico/genética , Triticum/genética , Triticum/metabolismo
20.
IEEE Trans Mol Biol Multiscale Commun ; 8(3): 138-157, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-36345554

RESUMO

Following recent advancements in Terahertz (THz) technology, THz communications are currently being celebrated as key enablers for various applications in future generations of communication networks. While typical communication use cases are over medium-range air interfaces, the inherently small beamwidths and transceiver footprints at THz frequencies support nano-communication paradigms. In particular, the use of the THz band for in-body and on-body communications has been gaining attention recently. By exploiting the accurate THz sensing and imaging capabilities, body-centric THz biomedical applications can transcend the limitations of molecular, acoustic, and radio-frequency solutions. In this paper, we study the use of the THz band for body-centric networks, by surveying works on THz device technologies, channel and noise modeling, modulation schemes, and networking topologies. We also promote THz sensing and imaging applications in the healthcare sector, especially for detecting zootonic viruses such as Coronavirus. We present several open research problems for body-centric THz networks.

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