Mammary fat globules as a source of mRNA to model alterations in the expression of some milk component genes during lactation in bovines.

BACKGROUND: The milk's nutritional value is determined by its constituents, including fat, protein, carbohydrates, and minerals. The mammary gland's ability to produce milk is controlled by a complex network of genes. Thereby, the fat, protein, and lactose synthesis must be boost in milk to increase milk production efficiency. This can be accomplished by fusing genetic advancements with proper management practices. Therefore, this study aimed to investigate the association between the Lipoprotein lipase (LPL), kappa casein CSN3, and Glucose transporter 1 (GLUT1) genes expression levels and such milk components as fat, protein, and lactose in different dairy breeds during different stages of lactation. METHODS: To achieve such a purpose, 94 milk samples were collected (72 samples from 36 multiparous black-white and red-white Holstein-Friesian (HF) cows and 22 milk samples from 11 Egyptian buffaloes) during the early and peak lactation stages. The milk samples were utilized for milk analysis and genes expressions analyses using non- invasive approach in obtaining milk fat globules (MFGs) as a source of Ribonucleic acid (RNA). RESULTS: LPL and CSN3 genes expressions levels were found to be significantly higher in Egyptian buffalo than Holstein-Friesian (HF) cows as well as fat and protein percentages. On the other hand, GLUT1 gene expression level was shown to be significantly higher during peak lactation than early lactation. Moreover, lactose % showed a significant difference in peak lactation phase compared to early lactation phase. Also, fat and protein percentages were significantly higher in early lactation period than peak lactation period but lactose% showed the opposite pattern of Egyptian buffalo. CONCLUSION: Total RNA can be successfully obtained from MFGs. The results suggest that these genes play a role in glucose absorption and lactose synthesis in bovine mammary epithelial cells during lactation. Also, these results provide light on the differential expression of these genes among distinct Holstein-Friesian cow breeds and Egyptian buffalo subspecies throughout various lactation phases.

High-performance phoxonic cavity designs for enhanced acousto-optical interaction.

Effective confinement of light and sound is achieved through a one-dimensional phoxonic crystal (PxC) cavity. In particular, co-localization of gigahertz phonons and infrared photons in a cavity created by introducing a defect inside a multilayer PxC has been performed. The incident elastic waves can control the refractive index variation of the dual phononic-photonic cavity layer. We also studied the acousto-optic (AO) effect in four AO materials, each located in the cavity layer between two identical Bragg mirrors. The cavities are designed to have high-quality factors for both photon and phonon resonances, which are proportional to their lifetime and allow for a much stronger photon-phonon interaction. The AO effect causes a shift in the optical mode of the photonic band gap. The values of the refractive index of the AO cavities layer are estimated as a function of time based on the elastic strain perturbation using the relevant photo-elastic relations. The phoxonic band gaps and transmission spectra for both unperturbed and elastically perturbed PxC structures are derived depending on the transfer matrix method. In our results, the selected AO cavity of PbMoO4 provided the strongest AO coupling, in which the maximum wavelength shift of the resonant photonic modes reached 113.3 nm. In addition, 11.9 nm is the maximum displacement amplitude of the confinement elastic wave of the same nanocavity. The TeO2 cavity provided the highest Q values for both photonic and phononic modes of 7093 and 175, respectively. We think this research could open the way to study the properties of linear elastic materials to design extremely miniaturized AO devices.

Determination of 1-propanol, ethanol, and methanol concentrations in water based on a one-dimensional phoxonic crystal sensor.

In this research, the photonic and phononic response of one-dimensional multilayer phoxonic crystals (PxCs) with normal incident of electromagnetic and acoustic waves is discussed. The presented design can work as a highly sensitive sensor for measuring three binary alcohol/water mixtures (i.e., 1-propanol/water, ethanol/water, and methanol/water) for a wide range of concentrations. The PxC sensor is able to detect small changes in the refractive index and longitudinal sound velocity of the alcohol/water mixture with initially neglecting the acousto-optical interaction. The sensor design is a defective structure as [$({\rm Si}/{\rm SiO}_2)^4 (\rm mixture\;wt. \%) {({{\rm SiO}_2}/{\rm Si})^4}$(Si/SiO2)4(mixturewt.%)(SiO2/Si)4]. Also, we studied the effects of changing mixture concentrations from 0 wt. % to 100 wt. % on the physio-chemical parameters and resonant mode frequency. In our results, we have achieved high performance for the three alcohol mixtures in both phononic and photonic sensors especially for low concentrations. For example, in the photonic sensor we obtained sensitivity, $Q$Q value, and figure of merit of 873 nm/RIU, 755, and ${290}\;{{\rm RIU}^{ - 1}}$290RIU-1, respectively, for methanol of concentration 10% in water. The phononic sensor showed higher results compared with the photonic sensor, as for ethanol with concentration 26.8% in water we obtained sensitivity, $Q$Q value, and figure of merit of ${37}\;{{\rm MHz/ms}^{ - 1}}$37MHz/ms-1, 1604, and ${8.4}\;{({\rm m/s})^{ - 1}}$8.4(m/s)-1, respectively. The proposed structure has different merits: operation at high temperatures, compact size, ease of fabrication, and feasibility of alcohol detection with two different methods that could be used in many chemical applications.