RESUMO
Heat transfer enhancement in dropwise condensation is widely investigated on a superhydrophobic surface with the advances in surface engineering, but the influence of a large amount of noncondensable gas (NCG) has not been clarified. In this work, the condensation heat transfer with a large amount of NCG is investigated by developing a multiphase lattice Boltzmann model for a multicomponent system. First, the condensation of a single droplet on a hydrophobic surface with NCG is simulated, demonstrating the capacity of the present model to capture the behaviors of different components during phase change and predict the significant influence of even a small fraction of the NCG on heat transfer. Then, solid surfaces with mixed wettability are built by introducing a fraction of hydrophilic parts to enhance heat transfer. It is found that there exists an optimized proportion which could maximize the condensation heat transfer efficiency corresponding to a specific mass fraction of NCG. Furthermore, the mechanism of this optimized proportion is revealed by examining the dynamic behaviors of condensation in a typical case, as a balance between a promotion of the nucleation rate and a put off of transition to filmwise condensation.
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Objective: To analyze the sperm morphological characteristics of multiple morphological abnormalities of the sperm flagella (MMAF), and to analyze their common features and subtypes. Methods: Twenty-eight patients with abnormal morphology of flagella were analyzed by semen analysis. The morphological characteristics were analyzed by scanning electron microscopy and transmission electron microscopy. Histological observation of one case of testicular tissue was performed. Results: Of the 28 patients, only 13 patients (46.4%) had motile spermatozoa, 12 of which had a sperm motility rate of <10% and a sperm survival rate of 9.0%-80.0%. Under light and scanning electron microscope, sperm with absent, short, coiled, bent and irregular width flagella or their combinations were observed. Transmission electron microscopy showed structural abnormalities of sperm fibrous sheath, mitochondrial sheath. Loss rate of central microtubule was 41.4%-84.6%. The semen of the 2 patients with the absence or presence of the kinetic protein arm and both the inner and lateral motilin arms missing had no motile spermatozoa. There was no statistically significant difference in the proportion of flagellar malformations between the two groups of patients (without motile sperm vs with motile spermatozoa). Conclusion: MMAF is a kind of sperm flagella specific abnormalities. Initially diagnosis can be carried out using light microscopy. Clear diagnosis could be conduct using transmission electron microscopy, and the central microtubule loss of the sperm could be seen as the main feature of the flagella abnormalities. Through the morphological analysis and research, MMAF could be precisely classified, which provide a strong basis for the diagnosis.
Assuntos
Infertilidade Masculina , Motilidade dos Espermatozoides , Cauda do Espermatozoide/patologia , Flagelos/patologia , Humanos , Masculino , Fenótipo , EspermatozoidesRESUMO
The aim of this study was to analyze the results of two crossing systems between wild boars and different domesticated pig breeds. Hybrid wild boars were produced by crossing captured wild boars with Meishan pigs and LY sows according to the traditional production system. The resultant commercial hybrids were black and white in coat color, respectively. Significant differences were found in the carcass and meat quality traits and nutritional values between these two hybrid wild boars. Compared with the white hybrid wild boars, at the age of 300 days, the body weight of black hybrid wild boars was 9.41 kg lower, while percent lean was 2.51% less and percent fat 2.45% higher (P < 0.05). The black hybrid wild boars had higher pH2 (6.17 vs 6.09) and intramuscular fat (3.34 vs 2.52%), lower drip loss (2.21 vs 2.68%) and shear force (44.00 vs 52.23) (P < 0.05), and more unsaturated fatty acids and essential amino acids (P < 0.05). In conclusion, cross breeding was shown to be an effective method to improve the overall production performance of wild boars, but crossing with different dam line breeds caused different responses. Compared with the white hybrid wild boars, the black hybrid wild boars had worse growth rate and carcass traits, but better meat quality traits and nutritional values.
Assuntos
Peso Corporal/genética , Hibridização Genética , Carne/análise , Valor Nutritivo , Sus scrofa/genética , Tecido Adiposo/metabolismo , Aminoácidos Essenciais/metabolismo , Animais , Composição Corporal/genética , Cruzamento/métodos , Cruzamentos Genéticos , Ácidos Graxos Insaturados/metabolismo , Feminino , Cor de Cabelo/genética , Masculino , Carne/normas , Locos de Características Quantitativas/genética , SuínosRESUMO
The myosin heavy chain (MyHC) composition, glycolytic potential, mitochondrial content, and gene expression related to energy metabolism were analyzed in eight muscles from Tibetan pigs, to study how meat quality develops in different muscle tissues. The muscles were classified into three clusters, based on MyHC composition: masseter, trapezius, and latissimus dorsi as 'slow-oxidative-type'; psoas major and semimembranosus as 'intermediate-type'; and longissimus dorsi, obliquus externus abdominis, and semitendinosus as 'fast-glycolytic-type'. The 'slow-oxidative-type' muscles had the highest MyHC I and MyHC IIA content (P < 0.01); 'intermediate-type' muscles, the highest MyHC IIx content (P < 0.01); and 'fast-glycolytic-type' muscles, the highest MyHC IIb content (P < 0.01). The pH values measured in 'slow-oxidative-type' muscles were higher than those in the other clusters were; however, the color of 'fast-glycolytic-type' muscles was palest (P < 0.01). Mitochondrial content increased in the order: fast-glycolytic-type < intermediate-type < slow-oxidative-type. In the 'slow-oxidative-type' muscles, the expression levels of genes related to ATP synthesis were higher, but were lower for those related to glycogen synthesis and glycolysis. Mitochondrial content was significantly positively correlated with MyHC I content, but negatively correlated with MyHC IIb content. MyHC I and mitochondrial content were both negatively correlated with glycolytic potential. Overall, muscles used frequently in exercise had a higher proportion of type I fibers. 'Slow-oxidative-type' muscles, rich in type I fibers with higher mitochondrial and lower glycogen and glucose contents, had a higher ATP synthesis efficiency and lower glycolytic capacity, which contributed to their superior meat quality.
Assuntos
Glicólise/genética , Carne , Fibras Musculares Esqueléticas/metabolismo , Cadeias Pesadas de Miosina/biossíntese , Miosina não Muscular Tipo IIB/biossíntese , Trifosfato de Adenosina/metabolismo , Animais , Metabolismo Energético/genética , Regulação da Expressão Gênica , Glicogênio/metabolismo , Mitocôndrias/genética , Mitocôndrias/metabolismo , Cadeias Pesadas de Miosina/genética , Miosina não Muscular Tipo IIB/genética , SuínosRESUMO
PURPOSE: We investigated correlations of somatic BRAF V600E mutation and RET/PTC1 rearrangement with recurrent disease in Chinese patients with papillary thyroid carcinoma (ptc). METHODS: This prospective study included 214 patients with ptc histologically confirmed between November 2009 and May 2011 at a single institute. RESULTS: We found somatic BRAF V600E mutation in 68.7% and RET/PTC1 rearrangement in 25.7% of the patients. Although BRAF mutation was not significantly associated with clinicopathologic features such as patient sex or age, multicentric disease, thyroid capsule invasion, tumour stage, or nodal metastasis, it was significantly associated with recurrent disease. Multivariate analysis revealed that BRAF mutation and tumour size were independent risk factors associated with recurrent disease, with odds ratios of 9.072 and 2.387 respectively. The area under the receiver operating characteristic curve increased 8.3% when BRAF mutation was added to the traditional prognostic factors, but that effect was statistically nonsignificant (0.663 vs. 0.746, p = 0.124). RET/PTC1 rearrangement and nodal metastasis were significantly associated in all patients (p = 0.042), marginally associated in ptc patients (p = 0.051), but not associated in microptc patients (p = 0.700). RET/PTC1 rearrangement was not significantly associated with recurrent disease. CONCLUSIONS: BRAF positivity is an independent predictor of recurrent disease in ptc.
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To explore the value of positron emission tomography-computed tomography (PET-CT) scan in esophageal squamous cell carcinoma (ESCC), we retrospectively summarize the results of PET-CT scan from 118 patients, with ESCC who underwent PET-CT scan in the different courses during treatment. Then, the results of PET-CT scan plus other conventional methods were analyzed to identify the value of PET-CT scan in diagnosis, staging, response evaluation, monitoring recurrence, and metastasis following treatment. It is suggested that PET-CT scan possess high value in diagnosis and gives more favorable indication in N and M staging. PET-CT scan should be translated into routine surveillance for postoperation follow up and is one of more helpful evaluators of response to chemoradiotherapy or chemotherapy.
Assuntos
Carcinoma de Células Escamosas/diagnóstico por imagem , Neoplasias Esofágicas/diagnóstico por imagem , Recidiva Local de Neoplasia/diagnóstico por imagem , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/diagnóstico , Estudos de Coortes , Neoplasias Esofágicas/diagnóstico , Carcinoma de Células Escamosas do Esôfago , Feminino , Fluordesoxiglucose F18 , Humanos , Masculino , Pessoa de Meia-Idade , Imagem Multimodal , Metástase Neoplásica , Recidiva Local de Neoplasia/diagnóstico , Estadiamento de Neoplasias , Tomografia por Emissão de Pósitrons , Compostos Radiofarmacêuticos , Estudos Retrospectivos , Tomografia Computadorizada por Raios XRESUMO
Contaminations of SARS-CoV-2 have been found for many times in imported cold chain aquatic products and their packaging materials in China. The purpose of this paper is to analyze the contamination status, sources and transmission risks of SARS-CoV-2 in imported cold chain aquatic products, and try to put forward relevant suggestions and strategies in order to provide evidence for the prevention and control of SARS-CoV-2 infection.
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COVID-19 , SARS-CoV-2 , China , Humanos , RefrigeraçãoRESUMO
Norovirus are now recognized as one of the main pathogens causing acute gastroenteritis in both developed and developing countries. However, norovirus are easily mutated and recombined, and have many genotypes. In early studies, norovirus were amplified and identified by amino acid sequence of VP1 region. It was found that norovirus were easily mutated and recombined in or near the overlapping regions of polymerase and capsid. A two regions genotyping method was positively proposed internationally. Depending on the 2 times standard deviation standard method for two regions identification, norovirus polymerase regions can be divided into 10 gene groups and 76 genotypes including 2 tentative gene groups and 16 tentative genotypes. The VP1 region can be divided into 12 gene groups and 53 genotypes including 2 tentative gene groups and 5 tentative genotypes. However, the tentative gene groups and genotypes need to be further identified and reclassified. In this article, characteristics of norovirus sequences, principles of different genotyping methods, methods of sequence amplification, on-line genotyping tools and the latest studies in norovirus genotypes are reviewed and introduced.
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Norovirus , Genótipo , Humanos , Norovirus/genéticaRESUMO
Vitiligo is a common pigment disease that is difficult to treat. The mechanism of repigmentation is not known. We combined Dopa-Toluidine Blue complex stain, hair follicle split-Dopa stain, and hair follicle split-scanning electron microscope (SEM) to observe the changes of melanocytes in 23 normal, 24 vitiliginous, and 36 repigmented skin specimens. We found that only active (Dopa-positive) melanocytes existed in the epidermis of normal skin. There were some inactive (Dopa-negative) melanocytes in the outer root sheaths of normal hair follicles, which form the melanocyte reservoir in human skin. In the patients with vitiligo the active melanocytes in the epidermis were totally missing, whereas the inactive melanocytes in the outer root sheaths of hair follicles were not affected. Treatment stimulated the inactive melanocytes in the middle and/or lower parts of the outer root sheaths of hair follicles to divide, proliferate, and migrate upward along the surface of the outer root sheath to the nearby epidermis, where the melanocytes continued to migrate radially to form the pigmented island visible clinically in repigmented vitiligo lesions. During the migration to the epidermis, the melanocytes matured gradually from an inactive phase to an active condition. In conclusion, the existence of these inactive melanocytes provided the melanocyte sources for repigmentation of vitiligo.
Assuntos
Cabelo/fisiologia , Pigmentação da Pele/fisiologia , Vitiligo/fisiopatologia , Biópsia , Di-Hidroxifenilalanina , Cabelo/ultraestrutura , Humanos , Melanócitos/fisiologia , Melanócitos/ultraestrutura , Microscopia Eletrônica de Varredura , Pele/patologia , Coloração e Rotulagem , ToluidinasRESUMO
Denervated muscle fibers exhibit spontaneous, repetitive single muscle fiber discharges and display fibrillation potentials detectable by electromyography. To explore the changing pattern of fibrillation potential amplitude after peripheral nerve injury and its relationship to the degree of muscle atrophy, fibrillation potential amplitudes were recorded on completely denervated biceps brachii of 173 patients with brachial plexus injury. Biceps brachii biopsies were taken at the same sites as the electromyogram recordings in 63 patients. The biopsies were analyzed by ATPase staining and the cross-sectional areas of fast and slow-twitch fibers were calculated. We found that the fibrillation potential amplitude and the cross-sectional areas of denervated muscle decay over time (P < 0.05), and both correlate negatively with denervation time (P < 0.01-0.05) within the first 15 months. The fibrillation potential amplitude correlates positively with both type I and II fiber cross-sectional areas (P < 0.0005-0.01). Our results show that fibrillation potential amplitude is closely correlated with muscle fiber size during the first 15 months after nerve injury, and it may therefore serve as a convenient index to evaluate quantitatively the degree of atrophy of denervated muscles. Electromyographic studies thus may help in designing treatment strategies.
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Denervação Muscular , Músculo Esquelético/fisiopatologia , Atrofia Muscular/fisiopatologia , Adolescente , Adulto , Eletromiografia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Fibras Musculares Esqueléticas/fisiologia , Músculo Esquelético/patologia , Atrofia Muscular/patologia , Fatores de TempoRESUMO
Toxoplasma gondii cysts in the tissues of experimentally infected mice and pigs were irradiated with cobalt-60 at various doses and used to infect mice and kittens. Loss of parasite infectivity was confirmed following irradiation whereas control animals inoculated with non-irradiated infected tissues became infected. Experiments were repeated to calculate the minimal effective dose (MED) of irradiation to eradicate parasite infectivity. The MED for the Chinese NT strain and the American ME-49 and TS-2 strains of T. gondii cysts in mouse and pig tissues was approximately 0.6 kGy. The infectivity for mice of NT strain bradyzoites irradiated at a dose of 0.45 kGy was reduced 10,000-fold. Such irradiation may be valuable in practical operations to control T. gondii in pork products.
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Radioisótopos de Cobalto , Toxoplasma/patogenicidade , Toxoplasma/efeitos da radiação , Toxoplasmose Animal/prevenção & controle , Animais , Encéfalo/parasitologia , Gatos , China , Relação Dose-Resposta à Radiação , Feminino , Camundongos , Especificidade da Espécie , Suínos , Estados Unidos , VirulênciaRESUMO
Monoamine oxidase type B (MAO-B) activity and free radicals are elevated in certain neurological diseases. Four natural flavonoids, quercitrin, isoquercitrin, rutin, and quercetin, were isolated for the first time from the leaves of Melastoma candidum D. Don. They exhibited an inhibitory effect on MAO-B. These potent flavonoids were purified using bioassay-guided fractionation and were separated by Diaion, Sephadex LH-20, and MCI CHP20P columns. The IC(50) values of the four potent flavonoids, quercitrin, isoquercitrin, rutin, and quercetin on monoamine oxidase were 19.06, 11.64, 3.89, and 10.89 microM and enzyme kinetics analysis revealed apparent inhibition constants (K(i)) of 21.01, 2.72, 1.83, and 7.95 microM, respectively, on the substrate, benzylamine. The four potent compounds also exhibited hydroxyl radical scavenging activity as determined using a spin trapping electron spin resonance method. This suggests that the four flavonoids from M. candidum possess both MAO-B inhibitory and free radical scavenging activities. These important properties may be used for preventing some neurodegenerative diseases in the future.
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Flavonoides/farmacologia , Sequestradores de Radicais Livres/farmacologia , Magnoliopsida/química , Inibidores da Monoaminoxidase/farmacologia , Monoaminoxidase/metabolismo , Cromatografia Líquida de Alta Pressão , Espectroscopia de Ressonância de Spin Eletrônica , Cinética , Espectrometria de FluorescênciaRESUMO
The causes and effects of increased intra-abdominal pressure and abdominal compartment syndrome have been well documented. However, there have been no large series to determine normal intra-abdominal pressure in hospitalized patients. The purpose of this study was to determine normal intra-abdominal pressure in randomly selected hospitalized patients and to identify factors that predict variation in normal intra-abdominal pressure. A total of 77 patients were prospectively enrolled between September 1998 and July 1999. Data obtained included patient demographics (i.e., age, gender, height, weight, and body mass index), reason for hospitalization and bladder catheterization, previous and current surgical status, comorbidities, and intra-abdominal pressures. Intra-abdominal pressure readings were obtained through an indwelling transurethral bladder (Foley) catheter. Data were analyzed by analysis of variance and multiple regression analysis. There were 36 females and 41 males with a mean age of 67.7 years. Average weight, height, and body mass index were 79.6 kg, 1.70 m, and 27.6 kg/m2, respectively. Mean intraabdominal pressure was 6.5 mm Hg (range 0.2-16.2 mm Hg). Body mass index was positively related to intra-abdominal pressure (P < 0.0004). Gender, age, and medical and surgical histories did not significantly affect intra-abdominal pressure. However, using multiple regression analysis, a relationship between intra-abdominal pressure, body mass index, and abdominal surgery was discovered. Intra-abdominal pressure is related to a patient's body mass index and influenced by recent abdominal surgery. Thus, the normal intra-abdominal pressure can be estimated in hospitalized patients by using the derived equation. Knowledge of the expected intra-abdominal pressure can then by used in recognizing when an abnormally high intra-abdominal pressure or abdominal compartment syndrome exists.
Assuntos
Abdome/fisiologia , Abdome/fisiopatologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Análise de Variância , Estatura , Índice de Massa Corporal , Peso Corporal , Comorbidade , Síndromes Compartimentais/diagnóstico , Síndromes Compartimentais/etiologia , Síndromes Compartimentais/fisiopatologia , Feminino , Humanos , Masculino , Manometria/instrumentação , Manometria/métodos , Pessoa de Meia-Idade , Distúrbios Nutricionais/fisiopatologia , Obesidade/fisiopatologia , Valor Preditivo dos Testes , Pressão , Estudos Prospectivos , Valores de Referência , Análise de Regressão , Cateterismo UrinárioRESUMO
The authors had designed cervical root nerve transfer from the contralateral normal side for the treatment of brachial plexus root avulsions in August 1986. Since then, the operation was performed in 50 cases. Follow-up studies showed that resection of C-7 root from the healthy side produced no long-term ill effect except numbness and pain of the fingers and weakness of gripping strength shortly after the procedure. In 10 recipient nerves of 9 cases with follow-ups of more than 2 years, satisfactory results were seen in 7. This is a new approach for the treatment of persistent cases of brachial plexus avulsion.
Assuntos
Plexo Braquial/lesões , Plexo Cervical/cirurgia , Transferência de Nervo , Adolescente , Adulto , Plexo Braquial/cirurgia , Criança , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
Since 1986, contralateral C7 root transfer has been used to treat brachial plexus root avulsions in our hospital. We performed post-operative electrophysiological examinations of the healthy-side limb in the first 27 patients. Electromyography and nerve conduction studies on the healthy side demonstrated mild abnormalities only of individual limb muscles in a few cases. These changes mostly recovered during the follow-up period. In the measurement of radial nerve motor conduction, median nerve sensory conduction and somato-sensory evoked potentials, we found that the amplitude of the median nerve sensory conduction alone was slightly lower than that of the control group (p < 0.05). The results showed that severance of C7 caused no permanent functional damage to the upper limb.
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Braço/inervação , Plexo Braquial/lesões , Potenciais Somatossensoriais Evocados , Transferência de Nervo , Condução Nervosa , Raízes Nervosas Espinhais/cirurgia , Adolescente , Adulto , Plexo Braquial/cirurgia , Eletromiografia , Feminino , Humanos , Masculino , Nervo Mediano/fisiopatologia , Complicações Pós-Operatórias , Nervo Radial/fisiopatologia , Ferimentos e Lesões/cirurgiaRESUMO
Shoulder abduction was studied in 25 cases of obstetric brachial plexus palsy (OBPP). According to muscle function, electromyographic features and X-ray examination, impairment of shoulder abduction could be classified into dynamic, resistant or combined types. Five cases were the dynamic type, which was characterized by paralysis of the shoulder abductors. Fifteen cases were categorized as the resistant type, with contracture of the subscapularis muscle, co-contraction of latissimus dorsi and teres major muscles and secondary disorders of the shoulder joint. Five cases were classified as the combined type in which there were both dynamic and resistant factors. Appropriate management and surgical procedures in the shoulder affected by OBPP depend on the pathological classification.
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Plexo Braquial/lesões , Músculo Esquelético/fisiopatologia , Paralisia Obstétrica/classificação , Paralisia Obstétrica/fisiopatologia , Articulação do Ombro/fisiopatologia , Adolescente , Criança , Pré-Escolar , Eletromiografia , Feminino , Humanos , Lactente , Masculino , Contração Muscular , Músculo Esquelético/patologia , Atrofia Muscular , Paralisia Obstétrica/patologia , Articulação do Ombro/patologiaRESUMO
Mouse brains harboring the Chinese NT strain of Toxoplasma gondii cysts were homogenized with normal saline and irradiated with cobalt-60 gamma rays at various doses. The homogenate was introduced intraperitoneally into NIH mice or per os into kittens. Loss of infectivity was confirmed according to the following criteria: no cyst found in mouse brain impression smears on the 50th day after inoculation; no oocyst found in feces of kittens 3-15 days after inoculation; subinoculation in mice and a negative IHA test. All bioassays, parasitological examinations and serological tests in the control group gave positive results. Activity of radioactive source: 10 KCi; uniform dosage: 1238 rad/min; dose range of irradiation: 0.1-1.0 KGy. Minimal effective dose of gamma rays to control infectivity of T. gondii cysts was 0.55 KGy. Infectivity of bradyzoites irradiated with gamma rays at a dose of 0.45 KGy decreased by 10,000 times. Minimal effective dose of gamma rays to control infectivity of American ME-49 and Ts-2 strain, is slightly higher (0.6KGy) than that of NT strain. These studies present useful data for practical use of cobalt-60 to control infectivity of T. gondii in meat products.