In vitro inhibition of α-Synuclein aggregation and disaggregation of preformed fibers by polyphenol hybrids with 2-conjugated benzothiazole.

The misfolding and aggregation of α-Syn play a pivotal role in connecting diverse pathological pathways in Parkinson's disease (PD). Preserving α-Syn proteostasis and functionality by inhibiting its aggregation or disaggregating existing aggregates using suitable inhibitors represents a promising strategy for PD prevention and treatment. In this study, a series of benzothiazole-polyphenol hybrids was designed and synthesized. Three identified compounds exhibited notable inhibitory activities against α-Syn aggregation in vitro, with IC50 values in the low micromolar range. These inhibitors demonstrated sustained inhibitory effects throughout the entire aggregation process, stabilizing α-Syn proteostasis conformation. Moreover, the compounds effectively disintegrated preformed α-Syn oligomers and fibers, potentially by binding to specific domains within the fibers, inducing fibril instability, collapse, and ultimately resulting in smaller-sized aggregates and monomers. These findings offer valuable insights into the therapeutic potential of polyphenol hybrids with 2-conjugated benzothiazole targeting α-Syn aggregation in the treatment of PD.

Fast Automatic Fuzzy C-Means Knitting Pattern Color-Separation Algorithm Based on Superpixels.

Patterns entered into knitting CAD have thousands or tens of thousands of different colors, which need to be merged by color-separation algorithms. However, for degraded patterns, the current color-separation algorithms cannot achieve the desired results, and the clustering quantity parameter needs to be managed manually. In this paper, we propose a fast and automatic FCM color-separation algorithm based on superpixels, which first uses the Real-ESRGAN blind super-resolution network to clarify the degraded patterns and obtain high-resolution images with clear boundaries. Then, it uses the improved MMGR-WT superpixel algorithm to pre-separate the high-resolution images and obtain superpixel images with smooth and accurate edges. Subsequently, the number of superpixel clusters is automatically calculated by the improved density peak clustering (DPC) algorithm. Finally, the superpixels are clustered using fast fuzzy c-means (FCM) based on a color histogram. The experimental results show that not only is the algorithm able to automatically determine the number of colors in the pattern and achieve the accurate color separation of degraded patterns, but it also has lower running time. The color-separation results for 30 degraded patterns show that the segmentation accuracy of the color-separation algorithm proposed in this paper reaches 95.78%.

Waveform Design for the Integrated Sensing, Communication, and Simultaneous Wireless Information and Power Transfer System.

Next-generation communication systems demand the integration of sensing, communication, and power transfer (PT) capabilities, requiring high spectral efficiency, energy efficiency, and low cost while also necessitating robustness in high-speed scenarios. Integrated sensing and communication systems (ISACSs) exhibit the ability to simultaneously perform communication and sensing tasks using a single RF signal, while simultaneous wireless information and power transfer (SWIPT) systems can handle simultaneous information and energy transmission, and orthogonal time frequency space (OTFS) signals are adept at handling high Doppler scenarios. Combining the advantages of these three technologies, a novel cyclic prefix (CP) OTFS-based integrated simultaneous wireless sensing, communication, and power transfer system (ISWSCPTS) framework is proposed in this work. Within the ISWSCPTS, the CP-OTFS matched filter (MF)-based target detection and parameter estimation (MF-TDaPE) algorithm is proposed to endow the system with sensing capabilities. To enhance the system's sensing capability, a waveform design algorithm based on CP-OTFS ambiguity function shaping (AFS) is proposed, which is solved by an iterative method. Furthermore, to maximize the system's sensing performance under communication and PT quality of service (QoS) constraints, a semidefinite relaxation (SDR) beamforming design (SDR-BD) algorithm is proposed, which is solved using through the SDR technique. The simulation results demonstrate that the ISWSCPTS exhibits stronger parameter estimation performance in high-speed scenarios compared to orthogonal frequency division multiplexing (OFDM), the waveform designed by CP-OTFS AFS demonstrates superior interference resilience, and the beamforming designed by SDR-BD strikes a balance in the overall performance of the ISWSCPTS.

Kaempferol promotes melanogenesis and reduces oxidative stress in PIG1 normal human skin melanocytes.

Vitiligo is an autoimmune disease characterized by depigmentation. Kaempferol is a flavonoid compound with broad anti-inflammatory and antioxidant properties. The purpose of this study was to investigate the effect of kaempferol on melanogenesis in PIG1 normal human skin melanocytes and its response to oxidative stress. The effect of kaempferol on melanin synthesis in PIG1 normal human skin melanocytes was explored by measuring tyrosinase activity, melanin content, mRNA and protein expression of key enzymes and expression of related pathway proteins. The effects of kaempferol pretreatment on cell viability, apoptosis, ROS level and HO-1 protein level under H2 O2 stimulation were explored. When treated with kaempferol, the tyrosinase activity and melanin content of PIG1 cells increased, the mRNA and protein expressions of TYR, TRP1, TRP2 and MITF increased, and the phosphorylation level of ERK1/2 increased. Upon the stimulation of H2 O2 , kaempferol reduced the production of ROS, decreased apoptosis and increased the protein expression of HO-1 in PIG1 cells. In addition, kaempferol inhibited oxidative stress-induced melanin reduction and promoted melanin synthesis in PIG1 cells and protected against H2 O2 -induced oxidative stress damage.

The Detection of Yarn Roll's Margin in Complex Background.

Online detection of yarn roll's margin is one of the key issues in textile automation, which is related to the speed and scheduling of bobbin (empty yarn roll) replacement. The actual industrial site is characterized by uneven lighting, restricted shooting angles, diverse yarn colors and cylinder yarn types, and complex backgrounds. Due to the above characteristics, the neural network detection error is large, and the contour detection extraction edge accuracy is low. In this paper, an improved neural network algorithm is proposed, and the improved Yolo algorithm and the contour detection algorithm are integrated. First, the image is entered in the Yolo model to detect each yarn roll and its dimensions; second, the contour and dimensions of each yarn roll are accurately detected based on Yolo; third, the diameter of the yarn rolls detected by Yolo and the contour detection algorithm are fused, and then the length of the yarn rolls and the edges of the yarn rolls are calculated as measurements; finally, in order to completely eliminate the error detection, the yarn consumption speed is used to estimate the residual yarn volume and the measured and estimated values are fused using a Kalman filter. This method overcomes the effects of complex backgrounds and illumination while being applicable to different types of yarn rolls. It is experimentally verified that the average measurement error of the cylinder yarn diameter is less than 8.6 mm, and the measurement error of the cylinder yarn length does not exceed 3 cm.

Design of Ultra-Wideband Doherty Power Amplifier Using a Modified Combiner Integrated with Complex Combining Impedance.

To be compatible with future wireless communication systems, it is very necessary to extend the bandwidth of the Doherty power amplifier (DPA). In this paper, a modified combiner integrated with a complex combining impedance is adopted to enable an ultra-wideband DPA. Meanwhile, a comprehensive analysis is performed on the proposed method. It is illustrated that the proposed design methodology can provide power amplifier (PA) designers with more freedom in implementing ultra-wideband DPAs. As a concept of proof, a DPA working over 1.2-2.8 GHz (a relative bandwidth of 80%) is designed, fabricated and measured in this work. Experimental results showed that the fabricated DPA delivers a saturation output power of 43.2-44.7 dBm with a gain of 5.2-8.6 dB. Meantime, the fabricated DPA achieves a saturation drain efficiency (DE) of 44.3-70.4% and a 6 dB back-off DE of 38.7-57.6%.

The Method of Creel Positioning Based on Monocular Vision.

Automatic replacement of bobbins on the frame is one of the core problems that must be solved in the textile industry. In the process of changing the bobbin, it is faced with problems such as the position offset of the bobbin, the loosening and deformation of the bobbin, which will lead to the failure of replacing the bobbin. Therefore, it is necessary to initialize the creel coordinates regularly, also considering the labor intensity and poor reliability of manual positioning. This paper proposes an automatic creel positioning method based on monocular vision. Firstly, the industrial camera was installed on the drum changing manipulator controlled by the truss system, and each yarn frame in the yarn area was inspected. Secondly, the end face image of the creel was collected at a reasonable distance, and the collected images were transmitted to the computer in real time through the Ethernet bus. Thirdly, the center coordinates (x, y) and radius r of the creel were marked by the improved Hough circle detection algorithm. Finally, the coordinate deviation of the creel was calculated and transmitted to the system controller to realize deviation correction. Before the test, the creel positioning markers were specially designed and the camera was calibrated. Thus, the influence of image complex background, creel end roughness, reflection and other factors can be reduced, and the image processing speed and positioning accuracy can be improved. The results show that the positioning effect of this method is fine when the distance between the center of the camera lens and the center of the end face of the creel is 170~190 mm. Moreover, when the distance is 190 mm, the positioning effect is the best, with an average error of only 0.51 mm. In addition, the deviation between the center coordinate and the radius of the end face of the marker is also very small, which is better than the requirements of bobbin yarn changing accuracy.

Crosstalk between N6-methyladenosine modification and circular RNAs: current understanding and future directions.

N6-methyladenosine (m6A) is a prevalent internal modification in eukaryotic RNAs regulated by the so-called "writers", "erasers", and "readers". m6A has been demonstrated to exert critical molecular functions in modulating RNA maturation, localization, translation and metabolism, thus playing an essential role in cellular, developmental, and disease processes. Circular RNAs (circRNAs) are a class of non-coding RNAs with covalently closed single-stranded structures generated by back-splicing. CircRNAs also participate in physiological and pathological processes through unique mechanisms. Despite their discovery several years ago, m6A and circRNAs has drawn increased research interest due to advances in molecular biology techniques these years. Recently, several scholars have investigated the crosstalk between m6A and circRNAs. In this review, we provide an overview of the current knowledge of m6A and circRNAs, as well as summarize the crosstalk between these molecules based on existing research. In addition, we present some suggestions for future research perspectives.

Identification of related impurities in an oral pharmaceutical formulation of tebipenem pivoxil using ultra-high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry.

RATIONALE: Tebipenem pivoxil (TBPM-PI) has been developed as the first oral carbapenem drug in the world to treat otolaryngological and respiratory infections in pediatric patients. Due to its structural properties and external factors, some related impurities, which may cause side effects in patients, might be formed during the synthesis and storage of TBPM-PI. It was vital to rapidly separate and identify the related impurities to guarantee the safe use of TBPM-PI. METHODS: A method using ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole time-of-flight tandem mass spectrometry (QTOF-MS/MS) was developed to separate and detect TBPM-PI and related impurities in an oral pharmaceutical formulation. LC/MS and MS/MS spectra of these compounds in the formulation were acquired to confirm their elemental compositions and propose their structures based on LC/MS data and fragmentation pathways of available reference substances. RESULTS: LC/MS parameters and MS/MS fragmentation pathways of reference substances of TBPM-PI and related impurities were summarized in detail. Based on this, a total of 23 related impurities were found and characterized in the oral pharmaceutical formulation. Eight of these were verified by comparison with reference substances and the structures of the other 15 were proposed for the first time. In addition, four of these compounds were produced by the reaction of excipients and pre-existing related impurities. CONCLUSIONS: A UHPLC/QTOF-MS method was established and used for the separation and identification of 23 related impurities in a TBPM-PI oral pharmaceutical formulation. Moreover, it was proved that new related impurities could be produced by the reaction of excipients in the pharmaceutical formulation and related impurities in the corresponding active pharmaceutical ingredient (API).

The Detection of Thread Roll's Margin Based on Computer Vision.

The automatic detection of the thread roll's margin is one of the kernel problems in the textile field. As the traditional detection method based on the thread's tension has the disadvantages of high cost and low reliability, this paper proposes a technology that installs a camera on a mobile robot and uses computer vision to detect the thread roll's margin. Before starting, we define a thread roll's margin as follows: The difference between the thread roll's radius and the bobbin's radius. Firstly, we capture images of the thread roll's end surface. Secondly, we obtain the bobbin's image coordinates by calculating the image's convolutions with a Circle Gradient Operator. Thirdly, we fit the thread roll and bobbin's contours into ellipses, and then delete false detections according to the bobbin's image coordinates. Finally, we restore every sub-image of the thread roll by a perspective transformation method, and establish the conversion relationship between the actual size and pixel size. The difference value of the two concentric circles' radii is the thread roll's margin. However, there are false detections and these errors may be more than 19.4 mm when the margin is small. In order to improve the precision and delete false detections, we use deep learning to detect thread roll and bobbin's radii and then can calculate the thread roll's margin. After that, we fuse the two results. However, the deep learning method also has some false detections. As such, in order to eliminate the false detections completely, we estimate the thread roll's margin according to thread consumption speed. Lastly, we use a Kalman Filter to fuse the measured value and estimated value; the average error is less than 5.7 mm.

Down-regulation of exosomal miR-200c derived from keratinocytes in vitiligo lesions suppresses melanogenesis.

Vitiligo is a refractory disfiguring skin disease. However, the aetiology and pathogenesis of vitiligo have not been fully defined. Previous studies have shown that exosomes from normal human keratinocytes improve melanogenesis by up-regulating the expression of melanogenesis-related proteins. Several microRNAs (miRNAs) have been demonstrated to be effective in modulating melanogenesis via exosomes. In the present study, it was found that the effect of exosomes derived from keratinocytes in vitiligo lesions in regulating melanin synthesis is weakened. Furthermore, miR-200c was detected to be significantly down-regulated in exosomes from keratinocytes in vitiligo lesions. In addition, miR-200c enhanced the expression of melanogenesis-related genes via suppressing SOX1 to activate ß-catenin. In conclusion, our study revealed that the effect of exosomes secreted by keratinocytes in vitiligo lesions exhibited a weaker capacity in promoting melanogenesis of melanocytes. Moreover, the expression of miR-200c, which mediates melanogenesis in exosomes secreted by keratinocytes in vitiligo lesions, is down-regulated, which may be one of the pathogenesis in vitiligo. Therefore, keratinocyte-derived exosomal miR-200c may be a potential target for the treatment of vitiligo.

Nucleophilic Aromatic Substitution of Unactivated Aryl Fluorides with Primary Aliphatic Amines by Organic Photoredox Catalysis.

In this work, a mild and transition-metal-free approach for the nucleophilic aromatic substitution (SN Ar) of unactivated fluoroarenes with primary aliphatic amines to form aromatic amines is reported. This reaction is facilitated by the formation of cationic fluoroarene radical intermediates in the presence of an acridinium-based organic photocatalyst under blue-light irradiation. Various electron-rich and electron-neutral fluoroarenes are competent electrophiles for this transformation. A wide range of primary aliphatic amines, including amino acid esters, dipeptides, and linear and branched amines are suitable nucleophiles. The synthetic utility of this protocol is demonstrated by the late-stage functionalization of several complex drug molecules.

Increased HERV-E clone 4-1 expression contributes to DNA hypomethylation and IL-17 release from CD4+ T cells via miR-302d/MBD2 in systemic lupus erythematosus.

BACKGROUND: Increased human endogenous retroviruses E clone 4-1 (HERV-E clone 4-1) mRNA expression is observed in systemic lupus erythematosus (SLE) patients and associates with the disease activity. In this study, we want to further investigate the mechanism of HERV-E clone 4-1 mRNA upregulation and its roles in SLE progression. METHODS: CD4+ T cells were isolated from venous blood of SLE patients or healthy controls and qRT-PCR was used to detect HERV-E clone 4-1 mRNA expression. We then investigated the regulation of Nuclear factor of activated T cells 1 (NFAT1) and Estrogen receptor-α (ER-α) on HERV-E clone 4-1 transcription and the functions of HERV-E clone 4-1 3' long terminal repeat (LTR) on DNA hypomethylation and IL-17 release. RESULTS: We found HERV-E clone 4-1 mRNA expression was upregulated in CD4+ T cells from SLE patients and positively correlated with SLE disease activity. This is associated with the activation of Ca2+/calcineurin (CaN)/NFAT1 and E2/ER-α signaling pathway and DNA hypomethylation of HERV-E clone 4-1 5'LTR. HERV-E clone 4-1 also takes part in disease pathogenesis of SLE through miR-302d/Methyl-CpG binding domain protein 2 (MBD2)/DNA hypomethylation and IL-17 signaling via its 3'LTR. CONCLUSIONS: HERV-E clone 4-1 mRNA upregulation is due to the abnormal inflammation/immune/methylation status of SLE and it could act as a potential biomarker for diagnosis of SLE. HERV-E clone 4-1 also takes part in disease pathogenesis of SLE via its 3'LTR and the signaling pathways it involved in may be potential therapeutic targets of SLE.

A Class-Selective Immunoassay for Sulfonamides Residue Detection in Milk Using a Superior Polyclonal Antibody with Broad Specificity and Highly Uniform Affinity.

The development of multianalyte immunoassays with an emphasis on food safety has attracted increasing interest, due to its high target throughput, short detection time, reduced sample consumption, and low overall cost. In this study, a superior polyclonal antibody (pAb) against sulfonamides (SAs) was raised by using a bioconjugate of bovine serum albumin with a rationally designed hapten 4-[(4-aminophenyl) sulfonyl-amino]-2-methoxybenzoic acid (SA10-X). The results showed that the pAb could recognize 19 SAs with 50% inhibition (IC50) below 100 µg L-1 and a recognition profile for SAs containing, either a five-atom ring or a six-atom ring, with highly uniform affinity. A three-dimensional quantitative structure-activity relationship analysis indicated that the electrostatic features of SAs play a considerably important role, during recognition with pAb than stereochemical effects. Skimmed milk samples were directly diluted five times before analysis. After optimization, the limit of detection for sulfamonomethoxine, sulfamethoxazole, sulfaquinoxaline, sulfadimethoxine, and sulfamethazine were 1.00, 1.25, 2.95, 3.35, and 6.10 µg L-1, respectively. The average recoveries for these 5 SAs were 72.0⁻107.5% with coefficients of variation less than 14.1%. The established method, based on pAb, with broad specificity and uniform affinity, offered a simple, sensitive, and high-throughput screening tool for the detection of multi-SAs in milk samples.

A preliminary study of growth characteristics of melanocytes co-cultured with keratinocytes in vitro.

To clarify the characteristic growth of melanocytes (MCs) and Keratinocytes (KCs) in vitro and discuss the mechanism of culturing autologous melanocytes in the treatment of vitiligo. Epidermis cells derived from normal skin tissues were isolated and cultured in vitro. Melanocytes in DOPA staining were observed. The expression level of markers in MCs was detected by qRT-PCR and the percentage of MCs and KCs were detected by flow cytometry. Cells derived from normal skin tissues mainly included KCs, MCs, and fibroblasts. There were significant differences between the percentage of KC, MC, fibroblasts (P < 0.05), and the expression of Microphthalmia-associated transcription factor (P < 0.05) and Tyrosinase-related protein-2 (P < 0.05) in the second, 10th, 20th, and 30th day. Significant differences were also found between the average numbers of MC stained by DOPA (P < 0.05) and the average percentage of MCs in the 10th, 20th, and 30th Day (P < 0.05). But there were no significant differences between the average percentage of KCs in the 10th, 20th, and 30th Day (P > 0.05) detected by flow cytometry. The number of MCs co-cultured with KCs in vitro reached the maximum in the 20th Day and this co-cultured model may contribute to the growth of MCs which could be used in the treatment of vitiligo.

Prevalence and genotype distribution of human papillomavirus among females in the suburb of Shanghai, China.

To describe the prevalence of human papillomavirus (HPV) and its genotype distribution among females in the suburb of Shanghai. A total of 33 562 participants were enrolled in this study from January to December 2016. HPV GenoArray test kit was used to perform HPV genotyping and was also used in DNA amplification and HybriBio's proprietary flow-through hybridization technique. The overall prevalence of HPV was 18.98% and the top ten genotypes of HPV infection were HPV 16 (3.36%), HPV 58 (2.65%), HPV 52 (2.48%), HPV 51 (1.58%), HPV 54 (1.40%), HPV 68 (1.32%), HPV 18 (1.23%), HPV 6 (1.15%), HPV 56 (1.10%), and HPV 33 (1.07%). Single infection (4749, 14.15%) was the most common types among all the infected cases. Significant differences were found among age groups and month groups in terms of simple and multiple infection (P < 0.05), pure HR, LR and mixed HPV infection (P < 0.05). The prevalence of HR and LR HPV infection among females in the suburb of Shanghai is high, prevalence of single and multiple infection, pure HR, LR and mixed infection is correlated with the age and month.

The down-regulation of hsa_circ_0012919, the sponge for miR-125a-3p, contributes to DNA methylation of CD11a and CD70 in CD4+ T cells of systemic lupus erythematous.

BACKGROUND: Systemic lupus erythematous (SLE) is an autoimmune disease characterized by the production of autoantibodies directed against various autoantigens. But the expression profiles and functions of circular RNAs (circRNAs) in SLE are still scarce. OBJECTIVES: To explore the roles of circRNA in SLE and its potential diagnostic potential in SLE. METHODS: SLE patients and healthy control subjects were recruited. CD4+ T cells were isolated, circRNA microarray analysis were used to screen for circRNA candidate in CD4+ T cells. Expression of DNMT1, CD11a and CD70, and methylation level of CD11a and CD70 were detected after transfecting hsa_circ_0012919-targetted siRNA. The network analysis of hsa_circ_0012919 was used by bioinformatics. Luciferase reporter assay and fluorescence in situ hybridization (FISH) assay were used for screening for which miRNAs could bind with hsa_circ_0012919. RESULTS: Twelve circRNAs were up-regulated and two circRNAs were down-regulated in SLE patients group after circRNA microarray analysis. Hsa_circ_0012919 was further confirmed to be significantly different between healthy control and SLE patients (P<0.05) and associated with SLE characters (P<0.05). Down-regulation of hsa_circ_0012919 (i) increased the expression of DNMT1 and reduced the expression of CD70, CD11a, (ii) reversed the DNA hypomethylation of CD11a and CD70 in CD4+ T cells of SLE, but it could be reversed by down-regulation of DNMT1. Hsa_circ_0012919 regulated KLF13 and RANTES by miR-125a Conclusion: Hsa_circ_0012919 could be regarded as a biomarker for SLE and hsa_circ_0012919 was the competitive endogenous RNA (ceRNA) for miR-125a-3p.

The Genotype of Human Papillomavirus and Associated Factors Among High Risk Males in Shanghai, China: A Molecular Epidemiology Study.

BACKGROUND The aim of this study was to describe the infection rate of human papillomavirus (HPV), its genotype distribution, and associated factors among high-risk males in Shanghai, China. MATERIAL AND METHODS A total of 1,205 participants were enrolled in this study from October 2015 to March 2017. Each individual signed an informed consent before agreeing to take part in this study; participants completed a questionnaire about associated factors related to HPV infection. The HPV GenoArray test kit was used to perform HPV genotyping and was also used in DNA amplification and HybriBio's proprietary flow-through hybridization technique. RESULTS The overall infection rate of HPV was 58.4%. HPV 11 (177, 14%) was the most common low-risk (LR) genotype and HPV 16 (78, 6.47%) was the top prevalent high-risk (HR) genotype among all the male patients. Additionally, multiple infections (387, 32.1%) was the most common type among all the infected cases, while single infection was only 317 cases (26.3%). Sexual preference, number of sex partners, sexual activity, and frequency of condom use were significantly associated with HR and LR infection. CONCLUSIONS The infection rate of HPV infection among high-risk males in Shanghai was very high.

Low Expression and Clinical Value of hsa_circ_0049224 and has_circ_0049220 in Systemic Lupus Erythematous Patients.

BACKGROUND The aim of this study was to discuss the possible roles and clinical value of 2 circRNAs (hsa_circ_0049224 and has_circ_0049220) in SLE patients. MATERIAL AND METHODS Reverse-transcription real-time polymerase chain reaction (RT-PCR) was conducted to detect the expressions of hsa_circ_0049224, has_circ_0049220, and DNMT1 in peripheral blood mononuclear cells (PBMCs) from 18 diagnosed SLE patients and 10 healthy controls. RESULTS We found that the expressions of hsa_circ_0049224 and has_circ_0049220 in healthy control groups were both much higher than those in inactive and active SLE patients. The expression level of DNMT1 is positively correlated with the expressions of hsa_circ_0049224 and has_circ_0049220. Moreover, there was a negative correlation between the SLE Disease Activity Index (SLEDAI) and the expressions of hsa_circ_0049224 and has_circ_0049220. We also found that these 2 circRNAs are associated with some clinical characteristics of SLE. CONCLUSIONS Hsa_circ_0049224 and has_circ_0049220 are probable factors involved in the pathogenesis of SLE, and they have potential clinical value in SLE.

Increase in Ceftriaxone Resistance and Widespread Extended-Spectrum ß-Lactamases Genes Among Salmonella enterica from Human and Nonhuman Sources.

Salmonella producing ß-lactamases has spread rapidly worldwide and poses a serious threat to human and animal health. In this study, we characterized 220 ceftriaxone (CRO)-resistant isolates identified among 3153 Salmonella from humans, animals, food, and water collected in Shanghai, China. They were assessed for antimicrobial susceptibility, phenotypic identification of extended-spectrum ß-lactamases (ESBLs), and ß-lactamase genes and integrons. CRO resistance in Salmonella increased from 5.0% in 2011 to 8.4% in 2013. Salmonella Enteritidis (45.5%), Salmonella Typhimurium (20.9%) from humans, and Salmonella Indiana (14.5%) from poultry represented the majority of the CRO-resistant isolates. Many isolates were also resistant to other antimicrobials, including nalidixic acid (84.5%), sulfisoxazole (70.5%), and tetracycline (61.8%). Resistance to ciprofloxacin was also found in 33.6% of the isolates. Most isolates (98.2%) were confirmed as ESBL producers. Resistance genes such as blaCTX-M, blaTEM, and blaOXA were detected in 207 (94.1%), 99 (45%), and 53 (24.1%) isolates, respectively. Three types of integron I and one type of integron II were identified in 13 (5.9%) and 2 (0.9%) isolates, respectively. The integrons encompassed 10 different genes: dfrA1/12/17/25, aadA1/2/5, sat2, orfF, and ybeA. Our study underscores concern for increasing CRO resistance, and highlights the widespread ESBL genes in Salmonella enterica.