RESUMO
Aeromonas salmonicida grown in a medium with excess glucose as carbon source produces both capsular and exocellular polysaccharides. The capsular polysaccharide is composed of glucose, mannose, rhamnose, N-acetylmannosamine and mannuronic acid in the molar ratios of approximately 5:3:0.75:2:1. The extracellular polysaccharide is similarly constituted, but in the molar ratios of approximately 4.75:10.5:1.5:2:1. The capsular and exocellular polysaccharides did not cross-react with monoclonal antibodies against the A-layer or the O-antigen lipopolysaccharide.
Assuntos
Aeromonas/química , Cápsulas Bacterianas/química , Aeromonas/imunologia , Aeromonas/patogenicidade , Anticorpos Antibacterianos , Anticorpos Monoclonais , Cápsulas Bacterianas/imunologia , Cápsulas Bacterianas/metabolismo , Polissacarídeos Bacterianos/biossíntese , Polissacarídeos Bacterianos/química , Polissacarídeos Bacterianos/imunologiaRESUMO
Pasteurella piscicida grown in a glucose-rich medium produces a capsule that can be see under light and electron microscopy. The capsular polysaccharide was purified and characterized by chemical and HPLC analysis. The polymer has the composition glucose/mannose/N-acetylgalactosamine/galacturonic acid/acetic acid in the molar ratios of approximately 2.5:1.3:0.5:0.4:2.5. The polysaccharide was immunogenic in rabbits and did not cross-react with antibodies against the O-antigen lipopolysaccharide.
Assuntos
Cápsulas Bacterianas/química , Pasteurella/química , Polissacarídeos Bacterianos/isolamento & purificação , Animais , Cápsulas Bacterianas/ultraestrutura , Cromatografia Líquida de Alta Pressão , Reações Cruzadas , Soros Imunes , Microscopia Eletrônica , Antígenos O , Pasteurella/crescimento & desenvolvimento , Pasteurella/imunologia , Polissacarídeos Bacterianos/imunologia , CoelhosAssuntos
Antibacterianos/farmacologia , Bactérias/isolamento & purificação , Contaminação de Medicamentos , Microbiologia de Alimentos , Metais/farmacologia , Pseudomonas aeruginosa/isolamento & purificação , Bactérias/efeitos dos fármacos , Resistência Microbiana a Medicamentos , Pseudomonas aeruginosa/efeitos dos fármacos , Sais/farmacologiaRESUMO
Extracellular polysaccharide (EPS) and capsular polysaccharide (CPS) production by Aeromonas salmonicida A450 and the influence of the capsule on cell surface properties were studied. A. salmonicida did not produce CPS or EPS when glucose, phosphate, magnesium chloride, or trace mineral components were absent from the medium. The addition of yeast extract improved capsule production. Neither EPS nor CPS formation depended on the C/N ratio, although it appeared to be influenced by the level of carbon and nitrogen in the culture. Both EPS and CPS production started at the end of the logarithmic growth phase. The amounts of EPS and CPS produced were not influenced by temperature changes between 15 and 20 degrees C and was maximal from pH 7 to 7.5. Cell surface properties were strongly influenced by capsule production; high CPS production was associated with enhanced cell hydrophilicity and autoagglutination. The effect of CPS on cell surface properties was independent of the presence of the surface protein array (A-layer).
Assuntos
Aeromonas/metabolismo , Polissacarídeos Bacterianos/biossíntese , Compostos de Potássio , Aeromonas/crescimento & desenvolvimento , Aeromonas/patogenicidade , Animais , Membrana Celular/metabolismo , Meios de Cultura , Doenças dos Peixes/etiologia , Peixes , Glucose , Cinética , Cloreto de Magnésio , Peptonas , Fosfatos , Potássio , Oligoelementos , VirulênciaRESUMO
Resting cells of Agrobacterium radiobacter EPS-916 grown on glucose, fructose, maltose, lactose, mannitol, or glycerol reduced 0.5 mM chromate. However, resting cells of strain EPS-916 grown on glutamate or succinate did not reduce chromate. The ability of washed cells to reduce chromate was correlated with their redox potential.
Assuntos
Actinomyces/isolamento & purificação , Actinomicose/microbiologia , Antígenos de Fungos/imunologia , Pneumonia/microbiologia , Actinomyces/classificação , Actinomyces/imunologia , Actinomicose/imunologia , Microbiologia do Ar , Poeira , Exposição Ambiental , Humanos , Pneumonia/imunologia , População UrbanaAssuntos
Bacteriólise , Microbiologia do Solo , Alcaligenes , Bacillus , Enterobacteriaceae , Escherichia coli , PseudomonasRESUMO
Se describe la identificacion y espectro de actividad litica de 103 cepas eubacterianas aisladas de diferentes suelos. Los microorganismos aislados se identificaron como pertenecientes a los generos Bacillus Pseudomonas y Achromobacter. Las cepas del genero Bacillus presentaron un mayor espectro de actividad litica siendo algunas de las identificadas com B. cereus las unicas que fueron activas sobre todas las bacterias ensayadas