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The process of crosslinking improves the physicochemical properties of biopolymer-based composites, making them valuable for biomedical applications. EDC/NHS-crosslinked collagen materials have a significant potential for tissue engineering applications, due to their enhanced properties and biocompatibility. Chemical crosslinking of samples can be carried out in several ways, which is crucial and has a direct effect on the final properties of the obtained material. In this study, the effect of crosslinking conditions on the properties of collagen films using EDC and NHS was investigated. Studies included FTIR spectroscopy, AFM, swelling and degradation tests, mechanical testing and contact angle measurements. Evaluation of prepared collagen films indicated that both crosslinking agents and crosslinking conditions influenced film properties. Notable alternations were observed in the infrared spectrum of the sample, to which EDC was added directly to the fish collagen solution. The same sample indicated the lowest Young modulus, tensile strength and breaking force parameters and the highest elongation at break. All samples reached the maximum swelling degree two hours after immersion in PBS solution; however, the immersion-crosslinked samples exhibited a significantly lower degree of swelling and were highly durable. The highest roughness was observed for the collagen film crosslinked with EDC, whereas the lowest was observed for the specimen crosslinked with EDC with NHS addition. The crosslinking agents increased the surface roughness of the collagen film, except for the sample modified with the addition of EDC and NHS mixture. All films were characterized by hydrophilic character. The films' modification resulted in a decrease in their hydrophilicity and wettability. Our research allows for a comparison of proposed EDC/NHS crosslinking conditions and their influence on the physicochemical properties of fish collagen thin films. EDC and NHS are promising crosslinking agents for the modification of fish collagen used in biomedical applications.
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Materiais Biocompatíveis , Colágeno , Reagentes de Ligações Cruzadas , Peixes , Animais , Reagentes de Ligações Cruzadas/química , Colágeno/química , Materiais Biocompatíveis/química , Resistência à Tração , Engenharia Tecidual/métodos , Espectroscopia de Infravermelho com Transformada de Fourier/métodos , Teste de Materiais , Carbodi-Imidas/químicaRESUMO
Melatonin and sericin exhibit antioxidant properties and may be useful in topical wound healing patches by maintaining redox balance, cell integrity, and regulating the inflammatory response. In human skin, melatonin suppresses damage caused by ultraviolet radiation (UVR) which involves numerous mechanisms associated with reactive oxygen species/reactive nitrogen species (ROS/RNS) generation and enhancing apoptosis. Sericin is a protein mainly composed of glycine, serine, aspartic acid, and threonine amino acids removed from the silkworm cocoon (particularly Bombyx mori and other species). It is of interest because of its biodegradability, anti-oxidative, and anti-bacterial properties. Sericin inhibits tyrosinase activity and promotes cell proliferation that can be supportive and useful in melanoma treatment. In recent years, wound healing patches containing sericin and melatonin individually have attracted significant attention by the scientific community. In this review, we summarize the state of innovation of such patches during 2021-2023. To date, melatonin/sericin-polymer patches for application in post-operational wound healing treatment has been only sparingly investigated and it is an imperative to consider these materials as a promising approach targeting for skin tissue engineering or regenerative dermatology.
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Melanoma , Melatonina , Sericinas , Cicatrização , Melatonina/uso terapêutico , Melatonina/farmacologia , Humanos , Cicatrização/efeitos dos fármacos , Melanoma/tratamento farmacológico , Melanoma/metabolismo , Melanoma/patologia , Animais , Sericinas/farmacologia , Sericinas/uso terapêutico , Antioxidantes/uso terapêutico , Antioxidantes/farmacologia , Neoplasias Cutâneas/tratamento farmacológico , Neoplasias Cutâneas/metabolismo , Neoplasias Cutâneas/patologiaRESUMO
This review article presents past and current alginate-based materials in each application, showing the widest range of alginate's usage and development in the past and in recent years. The first segment emphasizes the unique characteristics of alginates and their origin. The second segment sets alginates according to their application based on their features and limitations. Alginate is a polysaccharide and generally occurs as water-soluble sodium alginate. It constitutes hydrophilic and anionic polysaccharides originally extracted from natural brown algae and bacteria. Due to its promising properties, such as gelling, moisture retention, and film-forming, it can be used in environmental protection, cosmetics, medicine, tissue engineering, and the food industry. The comparison of publications with alginate-based products in the field of environmental protection, medicine, food, and cosmetics in scientific articles showed that the greatest number was assigned to the environmental field (30,767) and medicine (24,279), whereas fewer publications were available in cosmetic (5692) and food industries (24,334). Data are provided from the Google Scholar database (including abstract, title, and keywords), accessed in May 2023. In this review, various materials based on alginate are described, showing detailed information on modified composites and their possible usage. Alginate's application in water remediation and its significant value are highlighted. In this study, existing knowledge is compared, and this paper concludes with its future prospects.
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Alginatos , Polissacarídeos , Engenharia Tecidual , Água , Materiais BiocompatíveisRESUMO
This review provides a report on the properties and recent advances in the application of chitosan and chitosan-based materials in cosmetics. Chitosan is a polysaccharide that can be obtained from chitin via the deacetylation process. Chitin most commonly is extracted from cell walls in fungi and the exoskeletons of arthropods, such as crustaceans and insects. Chitosan has attracted significant academic interest, as well as the attention of the cosmetic industry, due to its interesting properties, which include being a natural humectant and moisturizer for the skin and a rheology modifier. This review paper covers the structure of chitosan, the sources of chitosan used in the cosmetic industry, and the role played by this polysaccharide in cosmetics. Future aspects regarding applications of chitosan-based materials in cosmetics are also mentioned.
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Quitosana , Cosméticos , Animais , Quitosana/química , Quitina/química , Polissacarídeos/química , Crustáceos/química , Materiais Biocompatíveis/químicaRESUMO
Chitosan (CS) has gained considerable attention due to its distinctive properties and its broad spectrum of potential applications, spanning cosmetics, pharmaceuticals, and biomedical uses. In this study, we characterized thin films comprising chitosan mixtures containing salicin (SAL) and glycerin (GLY), both with and without hyaluronic acid (HA) as active ingredients. Characterization was achieved through release studies of SAL, infrared spectroscopy, microscopy techniques (AFM and SEM), and thermogravimetric analysis (TGA). CS/GLY/SAL and CS/GLY/SAL/HA mixture films were fabricated using the solvent evaporation technique. We probed interactions between the components in the chitosan mixtures via infrared analysis. The concentration of released salicin was monitored at various time intervals in a phosphate buffer (PBS) at pH 5.5 using HPLC. The linear regression analysis for the calibration graph showed a good linear relationship (R2 = 0.9996) in the working concentration range of 5-205 mg/dm3. Notably, the release of SAL reached its peak after 20 min. Furthermore, the introduction of HA caused changes in the films' morphology, but their roughness remained largely unchanged. The results obtained were compared, indicating that the release of SAL in the CS mixture films is sufficient for diverse applications, including wound-healing materials and cosmetic beauty masks.
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Quitosana , Quitosana/química , Ácido Hialurônico/química , Glicerol , Álcoois BenzílicosRESUMO
In this work, the interactions between hyaluronic acid and bovine serum albumin were investigated. The film-forming properties of the mixture were proven, and the mechanical and surface properties of the films were measured. The results showed the interactions between hyaluronic acid and albumin, mainly by hydrogen bonds. Molecular docking was used for the visualization of the interactions. The films obtained from the mixture of hyaluronic acid possessed different properties to films obtained from the single component. The addition of bovine serum albumin to hyaluronic acid led to a decrease in the mechanical properties, and to an increase in the surface roughness of the film. The new materials that have been obtained by blending can form a new group of materials for biomedicine and cosmetology.
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Ácido Hialurônico , Soroalbumina Bovina , Ácido Hialurônico/química , Simulação de Acoplamento Molecular , Soroalbumina Bovina/química , Propriedades de SuperfícieRESUMO
Chitosan-collagen blends have been widely applied in tissue engineering, joints diseases treatment, and many other biomedical fields. Understanding the affinity between chitosan and collagen type II is particularly relevant in the context of mechanical properties modulation, which is closely associated with designing biomaterials suitable for cartilage and synovial fluid regeneration. However, many structural features influence chitosan's affinity for collagen. One of the most important ones is the deacetylation degree (DD) in chitosan and the hydroxylation degree (HD) of proline (PRO) moieties in collagen. In this paper, combinations of both factors were analyzed using a very efficient molecular dynamics approach. It was found that DD and HD modifications significantly affect the structural features of the complex related to considered types of interactions, namely hydrogen bonds, hydrophobic, and ionic contacts. In the case of hydrogen bonds both direct and indirect (water bridges) contacts were examined. In case of the most collagen analogues, a very good correlation between binding free energy and DD was observed.
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Quitosana , Quitosana/química , Simulação de Dinâmica Molecular , Colágeno Tipo II , Acetilação , Hidroxilação , ColágenoRESUMO
The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.
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Biopolímeros/química , Quitosana/química , Alicerces Teciduais/química , Animais , Materiais Biocompatíveis/química , Osso e Ossos/metabolismo , Adesão Celular , Linhagem Celular Tumoral , Colágeno/química , Reagentes de Ligações Cruzadas/química , Fibroínas/química , Humanos , Microscopia Eletrônica de Varredura , Osteoblastos/efeitos dos fármacos , Porosidade , Ratos , Medicina Regenerativa , Espectroscopia de Infravermelho com Transformada de Fourier , Engenharia Tecidual/métodosRESUMO
The lubrication mechanism in synovial fluid and joints is not yet fully understood. Nevertheless, intermolecular interactions between various neutral and ionic species including large macromolecular systems and simple inorganic ions are the key to understanding the excellent lubrication performance. An important tool for characterizing the intermolecular forces and their structural consequences is molecular dynamics. Albumin is one of the major components in synovial fluid. Its electrostatic properties, including the ability to form molecular complexes, are closely related to pH, solvation, and the presence of ions. In the context of synovial fluid, it is relevant to describe the possible interactions between albumin and hyaluronate, taking into account solution composition effects. In this study, the influence of Na+, Mg2+, and Ca2+ ions on human serum albumin-hyaluronan interactions were examined using molecular dynamics tools. It was established that the presence of divalent cations, and especially Ca2+, contributes mostly to the increase of the affinity between hyaluronan and albumin, which is associated with charge compensation in negatively charged hyaluronan and albumin. Furthermore, the most probable binding sites were structurally and energetically characterized. The indicated moieties exhibit a locally positive charge which enables hyaluronate binding (direct and water mediated).
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Cálcio/química , Ácido Hialurônico/química , Magnésio/química , Albumina Sérica Humana/química , Sódio/química , Água/química , Sítios de Ligação , Cátions Bivalentes , Cátions Monovalentes , Humanos , Ligação de Hidrogênio , Modelos Biológicos , Simulação de Dinâmica Molecular , Ligação Proteica , Conformação Proteica em alfa-Hélice , Domínios e Motivos de Interação entre Proteínas , Soluções , Líquido Sinovial/química , TermodinâmicaRESUMO
Collagen films are widely used as adhesives in medicine and cosmetology. However, its properties require modification. In this work, the influence of salicin on the properties of collagen solution and films was studied. Collagen was extracted from silver carp skin. The rheological properties of collagen solutions with and without salicin were characterized by steady shear tests. Thin collagen films were prepared by solvent evaporation. The structure of films was researched using infrared spectroscopy. The surface properties of films were investigated using Atomic Force Microscopy (AFM). Mechanical properties were measured as well. It was found that the addition of salicin modified the roughness of collagen films and their mechanical and rheological properties. The above-mentioned parameters are very important in potential applications of collagen films containing salicin.
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Álcoois Benzílicos/química , Colágeno/química , Glucosídeos/química , Animais , Peixes/metabolismo , Microscopia de Força Atômica/métodos , Reologia , Propriedades de SuperfícieRESUMO
Hydrogels are cross-linked networks of macromolecular compounds characterized by high water absorption capacity. Such materials find a wide range of biomedical applications. Several polymeric hydrogels can also be used in cosmetics. Herein, the structure, properties and selected applications of hydrogels in cosmetics are discussed in general. Detailed examples from scientific literature are also shown. In this review paper, most common biopolymers used in cosmetics are presented in detail together with issues related to skin treatment and hair conditioning. Hydrogels based on collagen, chitosan, hyaluronic acid, and other polysaccharides have been characterized. New trends in the preparation of hydrogels based on biopolymer blends as well as bigels have been shown. Moreover, biopolymer hydrogels employment in encapsulation has been mentioned.
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Biopolímeros/química , Cosméticos/química , Hidrogéis/química , Materiais Biocompatíveis , Humanos , Estrutura MolecularRESUMO
In this review, we provide a report on recent studies in the field of research on the blends of hyaluronic acid with other natural polymers, namely collagen and chitosan. Hyaluronic acid has attracted significant interest in biomedical and cosmetic applications due to its interesting properties. In recent years, blends of hyaluronic acid with other polymers have been studied for new materials development. New materials may show improved properties that are important in the biomedical applications and in cosmetic preparations. In this review paper, the structure, preparation, and properties of hyaluronic acid blends with collagen and chitosan have been discussed and examples of new materials based on such blends have been presented. A comparison of the currently available information in the field has been shown. Future aspects in the field of hyaluronic acid blends and their applications in the biomedical and cosmetic industry have also been mentioned.
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Tecnologia Biomédica , Ácido Hialurônico/química , Polímeros/química , Quitosana/química , Colágeno/químicaRESUMO
Materials such as polyvinyl chloride, polypropylene, and polyethylene are used for the construction of medical equipment, including inhalation equipment. Inhalation equipment, because of the wet conditions and good oxygenation, constitutes a perfect environment for microbial biofilm formation. Biofilms may affect microbiological cleanliness of inhalation facilities and installations and promote the development of pathogenic bacteria. Microbial biofilms can form even in saline environments. Therefore, the aim of this study was to evaluate the effect of medicinal brines on microbial biofilm formation on the surfaces of inhalation equipment. The study confirmed the high risk of biofilm formation on surfaces used in inhalation equipment. Isolated microorganisms belonged to potential pathogens of the respiratory system, which can pose a health threat to hospital patients. The introduction of additional contaminants increased the amount of bacterial biofilm. On the other hand, the presence of brines significantly limited the amount of biofilm, thus eliminating the risk of infections.
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Biofilmes/efeitos dos fármacos , Equipamentos e Provisões/microbiologia , Bactérias Gram-Negativas/isolamento & purificação , Bactérias Gram-Positivas/isolamento & purificação , Terapia Respiratória/instrumentação , Cloreto de Sódio/farmacologia , Biofilmes/crescimento & desenvolvimento , Contaminação de Equipamentos/prevenção & controle , Humanos , Polietileno , Polipropilenos , Cloreto de Polivinila , Propriedades de SuperfícieRESUMO
Miscibility is an important issue in biopolymer blends for analysis of the behavior of polymer pairs through the detection of phase separation and improvement of the mechanical and physical properties of the blend. This study presents the formulation of a stable and one-phase mixture of collagen and regenerated silk fibroin (RSF), with the highest miscibility ratio between these two macromolecules, through inducing electrostatic interactions, using salt ions. For this aim, a ternary phase diagram was experimentally built for the mixtures, based on observations of phase behavior of blend solutions with various ratios. The miscibility behavior of the blend solutions in the miscible zones of the phase diagram was confirmed quantitatively by viscosimetric measurements. Assessing the effects of biopolymer mixing ratio and salt ions, before and after dialysis of blend solutions, revealed the importance of ion-specific interactions in the formation of coacervate-based materials containing collagen and RSF blends that can be used in pharmaceutical, drug delivery, and biomedical applications. Moreover, the conformational change of silk fibroin from random coil to beta sheet, in solution and in the final solid films, was detected by circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR), respectively. Scanning electron microscopy (SEM) exhibited alterations of surface morphology for the biocomposite films with different ratios. Surface contact angle measurement illustrated different hydrophobic properties for the blended film surfaces. Differential scanning calorimetry (DSC) showed that the formation of the beta sheet structure of silk fibroin enhances the thermal stability of the final blend films. Therefore, the novel method presented in this study resulted in the formation of biocomposite films whose physico-chemical properties can be tuned by silk fibroin conformational changes by applying different component mixing ratios.
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Biopolímeros/química , Colágeno/química , Fibroínas/química , Seda/química , Animais , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/química , Biopolímeros/biossíntese , Bombyx/química , Microscopia Eletrônica de Varredura , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
The aim of this research was the modification of fish collagen films with various amounts of dialdehyde starch (DAS). Film properties were examined before and after the cross-linking process by DAS. Prepared biopolymer materials were characterized by Fourier Transform Infrared Spectroscopy and Atomic Force Microscopy. Moreover, the mechanical, thermal and swelling properties of the films were evaluated and the contact angle was measured. Research has shown that dialdehyde starch applied as a cross-linking agent influences collagen film properties. Mechanical testing indicated a decrease in Young's Modulus and an increase in breaking force, elongation at break, and tensile strength parameters. Results for contact angle were significantly higher for collagen films cross-linked with DAS; thus, the hydrophilicity of samples decreased. Modified samples presented a lower swelling degree in PBS than native collagen films. However, the highest values for the degree of swelling among the modified specimens were obtained from the 1% DAS samples, which were 717% and 702% for 1% and 2% collagen, respectively. Based on AFM images and roughness values, it was noticed that DAS influenced collagen film surface morphology. The lowest value of Rq was observed for 2%Coll_2%DAS and was approximately 10 nm. Analyzing thermograms for collagen samples, it was observed that pure collagen samples were less thermally stable than cross-linked ones. Dialdehyde starch is a promising cross-linking agent for collagen extracted from fish skin and may increase its applicability.
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Polymeric biomaterials are a widely used class of materials due to their versatile properties. However, as with all other types of materials used for biomaterials, polymers also have to interact with blood. When blood comes into contact with any foreign body, it initiates a cascade which leads to platelet activation and blood coagulation. The implant surface also has to encounter a thromboinflammatory response which makes the implant integrity vulnerable, this leads to blood coagulation on the implant and obstructs it from performing its function. Hence, the surface plays a pivotal role in the design and application of biomaterials. In particular, the surface properties of biomaterials are responsible for biocompatibility with biological systems and hemocompatibility. This review provides a report on recent advances in the field of surface modification approaches for improved hemocompatibility. We focus on the surface properties of polysaccharides, proteins, and synthetic polymers. The blood coagulation cascade has been discussed and blood - material surface interactions have also been explained. The interactions of blood proteins and cells with polymeric material surfaces have been discussed. Moreover, the benefits as well as drawbacks of blood coagulation on the implant surface for wound healing purposes have also been studied. Surface modifications implemented by other researchers to enhance as well as prevent blood coagulation have also been analyzed.
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In this study, the synthesis of poly(AVGVP) [where A-Alanine, V-Valine, G-Glycine, and P-Proline] is executed by the stepwise solution phase method. The interaction between Chitosan and synthetic polypentapeptide in blends was examined in the liquid and solid phases. Viscosity criteria that establish the total miscibility with Chitosan are the Δ[η]m, the intrinsic viscosity [η], Huggins coefficient [KH], by Garcia ΔB, α by Sun, and µ suggested by Chee, ΔK, and ß buttressed by Jiang and Han. Besides, the results are corroborated in the solid phase by differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). Miscibility in the blends led to higher thermal stability than that of pure polymers, according to thermogravimetric analysis (TGA). In vitro, studies offered the absence of cytotoxicity, and in vivo histopathological results advocated that the blend shows less inflammation and is more compact as against cotton gauge, evincing an enhanced healing environment and promising the possibility of use in wound therapeutic applications.
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The aim of the work is to compare the properties of nanohydroxyapatite coatings obtained using the electrophoretic deposition method (EDP) at 10 V, 20 V, and 30 V, and with deposit times of 2 and 5 min. The primary sedimentation was used to minimize the risk of the formation of particle agglomerates on the sample surface. Evaluation of the coating was performed by using a Scanning Electron Microscope (SEM), Energy-Dispersive Spectroscopy (EDS), Atomic Force Microscopy (AFM), optical profilometer, drop shape analyzer, and a nanoscratch tester. All of the coatings are homogeneous without any agglomerates. When low voltage (10 V) was used, the coatings were uniform and continuous regardless of the deposition time. The increase in voltage resulted in the formation of cracks in the coatings. The wettability test shows the hydrophilic behavior of the coatings and the mean contact angle values are in the range of 20-37°. The coatings showed excellent adhesion to the substrate. The application of a maximum force of 400 mN did not cause delamination in most coatings. It is concluded that the optimal coating for orthopedic implants (such as hip joint implants, knee joint implants or facial elements) is obtained at 10 V and 5 min because of its homogeneity, and a contact angle that promotes osseointegration and great adhesion to the substrate.
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In this study, we prepared composites made from polyvinyl alcohol (PVA), sodium alginate (SA), curcumin (Cur), and polydopamine (PD). The film-forming properties of the composites were researched for potential wound-healing applications. The structures of the polymer blends and composites were studied by FTIR spectroscopy and microscopic observations (AFM and SEM). The mechanical properties were measured using a Zwick Roell testing machine. It was observed that the formation of a polymeric film based on the blend of polyvinyl alcohol and sodium alginate led to the generation of pores. The presence of curcumin in the composite resulted in the alteration of the blend properties. After solvent evaporation, the polymeric blend of PVA, SA, and curcumin formed a stable polymeric film, but the film showed poor mechanical properties. The addition of polydopamine led to an improvement in the mechanical strength of the film and an increase in its surface roughness. A polymeric film of sodium alginate presented the highest surface roughness value among all the studied specimens (66.6 nm), whereas polyvinyl alcohol showed the lowest value (1.60 nm). The roughness of the composites made of PVA/SA/Cur and PVA/SA/Cur/PD showed a value of about 25 nm. Sodium alginate showed the highest values of Young's modulus (4.10 GPa), stress (32.73 N), and tensile strength (98.48 MPa). The addition of PD to PVA/SA/Cur led to an improvement in the mechanical properties. Improved mechanical properties and appropriate surface roughness may suggest that prepared blends can be used for the preparation of wound-healing materials.
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Chitosan is a polysaccharide with film-forming properties. Such properties are widely used for the preparation of beauty masks and wound-healing materials. In this work, chitosan-based films containing hyaluronic acid and rutin have been researched for potential cosmetic applications. Rutin was added to a chitosan solution in lactic acid, and then thin films were fabricated. The structure of the films was studied using FTIR spectroscopy. Surface properties were studied using an AFM microscope. The release of rutin from chitosan-based film was researched by the HPLC method. The properties of the skin, such as elasticity and moisturization, were studied using the Aramo TS 2 apparatus. It was found that the addition of rutin did not have an influence on the chitosan structure but affected its thermal stability. The roughness of the films was bigger after the addition of rutin to chitosan-based films. Skin elasticity and skin moisturization were somewhat improved after the topical application of the proposed chitosan-rutin mask. The maximum release of rutin was found after 20 min at pH 5.5, related to the pH of normal human skin. The average percentage of release from chitosan-based film containing hyaluronic acid was smaller than from chitosan-based films.