Biosorption as a method of biowaste valorization to feed additives: RSM optimization.

The aim of this work was to prepare an innovative microelemental feed additive for laying hens, based on waste biomass from the agricultural sector (alfalfa and goldenrod after CO2 extraction in supercritical state). The process was optimized by Response Surface Methodology (RSM) and the most favourable enrichment conditions were selected for Cu(II), Mn(II) and Zn(II) ions: pH - 5, sorbate concentration of Cu(II), Mn(II), Zn(II) - 10.0 mg/L for alfalfa and 10.7 mg/L for goldenrod and biomass dose - 0.1 g/L. Physicochemical properties of biomass were studied and functional groups involved in the binding of Cu(II), Mn(II), Zn(II) ions were determined (mainly carboxylic and hydroxylic groups). An interesting and unique element of this work is the verification of the properties of prepared feed additives in conditions simulating the digestive tract of animals. The release of components in solutions simulating conditions in the intestine and stomach (pH 11 and pH 1) was tested (in vitro tests). The best desorption results were achieved at a strongly acidic pH which corresponds to the stomach environment: 9.80, 14.4% Cu(II), 69.0, 66.9% (Zn), 46.5, 31.9 Mn(II) for alfalfa and goldenrod, respectively. It was concluded that the biomass enriched with micronutrients in biosorption has the potential as a feed additive for sustainable agriculture.

Phytochemicals containing biologically active polyphenols as an effective agent against Covid-19-inducing coronavirus.

The outbreak of Covid-19 disease caused by SARS-CoV-19, along with the lack of targeted medicaments and vaccines, forced the scientific world to search for new antiviral formulations. In this review, we describe the current knowledge about plant extracts containing polyphenols that inhibit Covid-19. Many plant-derived natural compounds (polyphenols) might provide a starting point for the research on the use of plant extracts in coronavirus treatment and prevention. Antivirus polyphenolic drugs can inhibit coronavirus enzymes, which are essential for virus replication and infection. This group of natural substances (betulinic acid, indigo, aloeemodine, luteolin, and quinomethyl triterpenoids, quercitin or gallates) is a potential key to designing antiviral therapies for inhibiting viral proteases. The known pharmacophore structures of bioactive substances can be useful in the elaboration of new anti-Covid-19 formulations. The benefit of using preparations containing phytochemicals is their high safety for patients and no side effects.