RESUMO
Antibodies reacting with the cytoplasm of epidermal cells (E. C. A.) were defected by indirect immunofluorescence (I.I.F.) studies of 255 sera in various dermatoses (8 p. 100) and significantly more frequently (46 p. 100) in bone marrow transplanted patients having skin lesions. Bound E.C.A. were only found in two cases. E.C.A. were found to be devoided of any diagnostic value. E.C.A. were interpreted as the result of a skin injury liberating skin antigens which stimulated their production. The reaction of E.C.A. in I.I.F. on human skins substrates showed three different patterns; in some cases only the basal cell layer was stained; in others only the supra basal layers were stained; in other all the layers were stained. A serum was shown to be able to give the three patterns of reaction (or no reaction at all) if different human skins were used as substrate. Autologous skins never gave a basal cell layer staining. These findings support the facts that (I) there is antigenic differences between the basal cell layer and the supra basal layers (with regard to the cytoplasmic antigens) (II) these antigens may be different from one subject to the other, supporting the allogeneic nature of this system. Therefore, E.C.A. are regarded as a useful marker of the human skin cytoplasmic antigens.
Assuntos
Anticorpos/isolamento & purificação , Citoplasma/imunologia , Pele/imunologia , Animais , Células da Medula Óssea , Transplante de Medula Óssea , Esôfago/imunologia , Imunofluorescência , Humanos , Fígado/imunologia , Coelhos/imunologia , Pele/ultraestrutura , Transplante HomólogoAssuntos
Circulação Coronária/efeitos dos fármacos , Doença das Coronárias/fisiopatologia , Flumazenil/uso terapêutico , Coração/efeitos dos fármacos , Cateterismo Cardíaco , Doença das Coronárias/tratamento farmacológico , Método Duplo-Cego , Eletrocardiografia , Humanos , Miocárdio/metabolismo , Consumo de Oxigênio/efeitos dos fármacos , Ensaios Clínicos Controlados Aleatórios como AssuntoAssuntos
Período de Recuperação da Anestesia , Benzodiazepinas/antagonistas & inibidores , Flumazenil/farmacologia , Período Pós-Operatório , Adulto , Idoso , Analgésicos , Anestesia Geral , Diazepam/antagonistas & inibidores , Avaliação de Medicamentos , Feminino , Flunitrazepam/antagonistas & inibidores , Humanos , Masculino , Midazolam/antagonistas & inibidores , Pessoa de Meia-Idade , Estudos Prospectivos , Distribuição Aleatória , Respiração/efeitos dos fármacosRESUMO
HL-A antisera were found to react, in indirect immunofluorescence on human skin, with the cytoplasm of the upper cell layers of the epidermis. It has been established that this staining was not due to an HL-A-antigen-antibody reaction but rather to a contamination of HL-A antisera with non-HL-A epidermal cytoplasmic antibodies. These findings ruled out a previous hypothesis that HL-A antigens were expressed in the cytoplasm of the upper epidermal cell layers. The localization of HL-A antigens in the human epidermis has not been demonstrated by use of indirect immunofluorescence.