Comparative Analysis of MMP-8 and MMP-9 Concentrations in Crevicular and Peri-Implants Sulcular Fluids.

The concentrations of MMP-8 and MMP-9 were measured in the crevicular and the peri-implant sulcular fluids at the different stages of the prosthetic treatment. The concentration MMP-8 and MMP-9 in the peri-implant sulcular fluids were significantly higher (p<0.05) then in the gingival crevicular fluid. The determined parameters are the references values for dynamic observation over the functional state of the dental "implant-bone-soft" tissue system. The detected the correlations attest to synergy between secretion of MMP-8 and MMP-9 in the peri-implant sulcular fluid and allow analysis of the dependence of the secretion of these metalloproteinases on clinical and physiological peculiarities of the gingiva, which will help to better customize implant-supported prosthetic treatment of the patients.

Efficiency and Prospects of Diagnosis of the Functional State of the Dental System.

This paper presents the results of studying the effectiveness of the developed method for diagnosing the functional state of the dental system. The results indicate that the accuracy of the method is about 80%. The results of previous studies are reviewed and the prospects for diagnosis of the functional state of the dental system as a research and practical trend in dentistry are outlined. The physiological regularities of the dental system functioning and relationship between its functional status and the autonomic nervous and postural systems prompt a new trend of research, functional diagnosis in dentistry and interpretation of the findings of functional studies. The prospects of functional diagnosis in dentistry are discussed.

Coordination of the stomatognathic and postural system activities and their functional status.

Analysis of correlations of the functional parameters of the stomatognathic and postural systems with sub-, decompensated, and compensated status was carried out. Disorders in the masticatory muscle activity and interactions between the postural and stomatognathic systems were detected in patients with sub- and decompensated functional status. A significant correlation between the studied functional parameters was detected in the patients with the compensated functional status.

Matrix metalloproteinases and inflammatory cytokines in the oral fluid of patients with chronic generalized periodontitis various structural materials restoration of teeth and dentition.

Results of comparative immunoenzymatic study of matrix metalloproteinase (MMP) 2, 8 and 9, interleukins (IL) If and 6, tissue MMP inhibitors (TIMP-1and TIMP-2) and TNF-a in oral fluid of patients with different teeth and denture constructive materials show that MMP-9 content in oral fluid can serve as a marker of chronic generalized periodontitis because it is elevated in all patients irrespective of presence or absence of metallic tooth restorations. MMP-8 level is elevated as compared to control only in periodontitis patients with metallic restorations. The character of correlative relationships between the parameters studied in various patients'groups demonstrate relative similarity of MMP, IL and TIMP secretion regulation in patients with intact periodontal. In patients with inflammatory destructive periodontal lesions both with and without metallic restorations the correlation data reveal a cascade of biochemical reactions in response to etiologic factors. More pronounced response is observed in periodontitis patients with metallic orthopedic constructions. The presence of chromium-cobalt or chromium-nickel constructions leads to an increase of MMP-2, IL-lf and IL-6 content in oral fluid.

Classification of patients by the function of dentition, postural, and autonomic nervous systems.

Cluster analysis of the results of studies of the functional status of the dentition postural and autonomic nervous systems in 251 patients (129 men and 122 women) aged 20-60 years was carried out. European stabilometry variant was used. The patients could be distributed into two groups by the results of cluster analysis. By functional parameters, groups 1 and 2 could be defined as patients with decompensated and compensated functional status, respectively. The characteristics of the functional status of patients in the two groups are presented.

Information value of functional status of the stomatognathic system for postural balance regulation.

We performed cluster analysis of stabilometry data of 129 men and 122 women at the age of 20-60 years using the following 4 methods: European stabilometry variant, Romberg test (European variant), Romberg test (American variant), and Romberg test (American Universal variant). Results of factor analysis of primary data matrix obtained during the test "eye open-teeth clenched" attest to higher clinical significance of absolute stabilometry parameters in comparison with relative ones. The results of factor analysis revealed peculiarities of functional status of the stomatognathic system as the postural sensor and its effect on postural balance. The contribution of the functional status of the stomatognathic system into the postural balance is about 2%.

Matrix metalloproteinases and inflammatory cytokines in oral fluid of patients with chronic generalized periodontitis and various construction materials.

We present the results of comparative enzyme-linked immunosorbent assay of matrix metalloproteinases MMP-2, MMP-8, MMP-9; IL-1ß and IL-6; tissue inhibitors of MMP (TIMP-1, TIMP-2), and TNF-α. The object of study was oral fluid of practically healthy subjects with intact periodontium and patients with chronic generalized periodontitis with various structural materials of dental restorations. It was found that MMP-9 in oral fluid could be considered as a marker of chronic generalized periodontitis irrespective of the presence or absence of metal dental restorations. The level of MMP-8 surpassed the normal only in oral fluid of patients with chronic generalized periodontitis with metal restorations. The correlations between the studied parameters in patients attest to relatively similar regulation of MMP, IL and TIMP secretion in oral fluid in practically healthy subjects with intact periodontium. In patients with inflammatory destructive periodontal diseases with and without metal dental restorations, the correlation coefficients indicate initiation of biochemical cascade accompanied by activation of cytokine production in response to etiological factors. Patients with periodontitis and metal orthodontic structures showed more pronounced reaction. Orthodontic structures made from chromium-cobalt, or chromium-nickel alloys in the oral cavity of these patients increased the levels of MMP-2, IL-1ß and IL-6 in oral fluid.

Comparative analysis of methods for evaluation of stabilometry parameters.

Cluster analysis of stabilometry results in 251 subjects (129 men and 122 women, age 20-60 years) was carried out. Four methods were used in the study: European stabilometry variant, Romberg test (European variant), Romberg's test (American variant), and Romberg's test (American Universal variant). Cluster analysis has shown virtually the same diagnostic informative value of all 4 methods. The absolute and relative stabilometry parameters are the most objective, this validating their clinical usefulness. The frequency and amplitude parameters possess no diagnostic significance. The European stabilometry variant is the best fit for practical use and provides information about the function of the ocular, maxillofacial, and other components of the postural system.

Content of matrix metalloproteinase-8 and matrix metalloproteinase-9 in oral fluid of patients with chronic generalized periodontitis.

The content of MMP-8 and MMP-9 in oral fluid of 145 patients (95 women and 50 men, 18-52 years) was measured by enzyme immunoassay. We examined 63 subjects with the intact periodontium and 82 patients with chronic generalized periodontitis (25 patients with mild disease, 45 patients with moderate disease, and 12 patients with severe disease). All patients were examined during the remission of chronic periodontitis and did not have clinical signs of associated somatic diseases. No significant differences were found in the content of MMP-8 and MMP-9 in oral fluid from periodontitis patients and subjects with the intact periodontium. The content of MMP-8 an MMP-9 in oral fluid of patients with severe periodontium was slightly higher than that in other patients and subjects with the intact periodontium. The depth of the periodontal pocket was shown to be the most reliable clinical sign for the state of periodontal tissues. A strong correlation was revealed between this criterion and MMP-9 content in oral fluid.