Enhanced bioconversion of dairy and chicken manure by the interaction of exogenous bacteria and black soldier fly larvae.

Generation of insects' biomass from lignocellulose rich organic wastes is of significant challenges in reducing the environmental impact of wastes and in sustaining feed and food security. This research looked at the effects of lignocellulotic exogenous bacteria in the black soldier fly (BSF) organic waste conversion system for biomass production and lignocellulose biodegradation of dairy and chicken manures. Six exogenous bacteria were investigated for cellulolytic activity with carboxymethyl cellulose and found that these tested bacterial strains degrade the cellulose. In this study; a co-conversion process using Hermetia illucens larvae to convert the previously studied best mixing ratio of dairy manure (DM) and chicken manure (CHM) (2:3) and cellulose degrading bacteria was established to enhance the larval biomass production, waste reduction and manure nutrient degradation. BSF larvae assisted by MRO2 (R5) has the best outcome measures: survival rate (99.1%), development time (19.0 d), manure reduction rate (48.7%), bioconversion rate (10.8%), food conversion ratio (4.5), efficiency of conversion of ingestion (22.3), cellulose (72.9%), hemicellulose (68.5%), lignin (32.8%), and nutrient utilization (protein, 71.2% and fat, 67.8%). By analyzing the fiber structural changes by scanning electron microscopy and Fourier-transformed infrared spectroscopy (FT-IR), we assume that exogenous bacteria assist the BSF larvae that trigger lead to structural and chemical modification of fibers. We hypothesized that these surface and textural changes are beneficial to the associated gut bacteria, thereby helping to larval growth and reduce waste. The finding of the investigation showed that enhanced conversion of DM and CHM by BSF larvae assisted with lignocellulotic exogenous bacteria could play key role in the manure management.

Influence of Lactobacillus buchneri on soybean curd residue co-conversion by black soldier fly larvae (Hermetia illucens) for food and feedstock production.

Black soldier fly larvae (BSFL), Hermetia illucens (Diptera: Stratiomyidae) can reduce environmental pollution and convert organic wastes into biomass that is rich in protein and fat. The influence of the nutritional characteristics of organic waste on BSFL characteristics relevant for food and feed safety remains poorly understood. To evaluate the conversion of soybean curd residues (SCR) into high-quality animal-derived proteins and fats for human and livestock consumption, this study assessed the co-conversion efficacy, nutrient composition, safety, and anti-nutritional factor concentrations in BSFL after the development on SCR with Lactobacillus buchneri (L3-9). SCR was pretreated with L. buchneri (108 cfu/ml), and then BSFL was employed for conversion. BSFL fed with SCR and L. buchneri had a significantly higher dry mass reduction (55.7 ±â€¯0.9%), bioconversion rate (6.9 ±â€¯0.3%), crude protein content (55.3 ±â€¯0.6%), and fat content (30.0 ±â€¯0.6%) than SCR (49.0 ±â€¯0.7%, 5.0 ±â€¯0.3%, 52.8 ±â€¯0.3%, and 26.1 ±â€¯0.8%, respectively) and artificial feed (43.9 ±â€¯0.8%, 3.9 ±â€¯0.1%, 50.3 ±â€¯0.4%, and 24.3 ±â€¯0.4%, respectively). However, the feed conversion ratio (8.0 ±â€¯0.3), of BSFL fed with SCR and L. buchneri was lower than that of the BSFL fed with SCR (9.8 ±â€¯0.1) and artificial feed (11.1 ±â€¯0.5). In addition, BSFL had satisfactory concentrations of all essential amino acids and fatty acids required for human consumption as recommended by WHO/FAO/UNU. The heavy metals and anti-nutritional factor concentrations were within the safety intake levels for food and feedstock. Therefore, the addition of L. buchneri with BSFL on SCR did not only increase co-conversion performance but also enhanced the nutritional value of BSFL.