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Flowering time is of great agricultural importance and the timing and extent of flowering usually determines yield and availability of flowers, fruits and seeds. Identification of genes determining flowering has important practical applications for tomato breeding. Here we demonstrate the roles of the FANTASTIC FOUR (FAF) gene family in regulating tomato flowering time. In this plant-specific gene family, SlFAF1/2a shows a constitutive expression pattern during the transition of the shoot apical meristem (SAM) from vegetative to reproductive growth and significantly influences flowering time. Overexpressing SlFAF1/2a causes earlier flowering compared with the transformations of other genes in the FAF family. SlFAF1/2c also positively regulates tomato flowering, although to a lesser extent. The other members of the SlFAF gene family, SlFAF1/2b, SlFAF3/4a and SlFAF3/4b, are negative regulators of tomato flowering and faf1/2b, faf3/4a and faf3/4b single mutants all display early flowering. We generated a series of early flowering mutants using the CRISPR/Cas9 editing system, and the faf1/2b faf3/4a faf3/4b triple mutant flowering earliest compared with other mutants. More importantly, these mutants show no adverse effect on yield. Our results have uncovered the role of the FAF gene family in regulating tomato flowering time and generated early flowering germplasms for molecular breeding.
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Solanum lycopersicum , Solanum lycopersicum/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sistemas CRISPR-Cas/genética , Melhoramento Vegetal , Mutação/genética , Flores , Regulação da Expressão Gênica de Plantas/genéticaRESUMO
The degree of stigma exsertion has a major influence on self-pollination efficiency in tomato, and its improvement is essential for raising productivity and for fixing advantageous traits in cultivated tomato. To study the evolution of stigma exsertion degree in tomato, we searched for genes associated with this trait and other aspects of flower morphology, including the lengths of anthers, styles, and ovaries. We performed a genome-wide association on 277 tomato accessions and discovered a novel stigma exsertion gene (SE3.1). We reannotated the structure of the gene, which encodes a C2H2-type zinc finger transcription factor. A mutation of the lead single nucleotide polymorphism creates a premature termination codon in SE3.1 and an inserted stigma in cultivated tomatoes. SE3.1 is essential for the conversion of flush stigmas to inserted stigmas. This conversion has a major impact on the rate of self-fertilization. Intriguingly, we found that both SE3.1 and Style2.1 contribute to the transition from stigma exsertion to insertion during the domestication and improvement of tomato. Style2.1 controls the first step of exserted stigmas to flush stigmas, and SE3.1 controls the second step of flush stigmas to inserted stigmas. We provide molecular details for the two-step process that controls the transition from stigma exsertion to insertion, which is of great agronomic importance in tomato.
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Estudo de Associação Genômica Ampla , Proteínas de Plantas/genética , Polinização/genética , Solanum lycopersicum/fisiologia , Fatores de Transcrição/genética , Solanum lycopersicum/genética , Mutação , Proteínas de Plantas/metabolismo , Fatores de Transcrição/metabolismoRESUMO
OBJECTIVE: Previous studies have revealed associations between disordered eating symptoms, big-five personality traits, and psychological distress. However, limited research has explored these relationships as a network, including their interconnections, and even less has done so in non-Western populations. We employed network analysis to investigate the co-occurrence of disordered eating symptoms, big-five personality traits, and psychological distress in Chinese adults. METHOD: A sample of 500 Chinese adults (256 men) completed measures assessing big-five personality traits, psychological distress, and disordered eating symptoms. The network of personality traits, psychological distress, and disordered eating symptoms was estimated, including its central and bridge nodes. RESULTS: The central nodes in the network were the facets of openness (like adventure), extraversion (like going to social and recreational parties), and disordered eating symptoms (dissatisfaction with body weight or shape). Moreover, certain facets of neuroticism (always worrying something bad will happen), psychological distress (feeling worthless), and an inverse facet of extraversion (bored by parties with lots of people) were identified as essential bridge nodes in maintaining the structure of the network. CONCLUSION: Our findings suggest that personality traits (e.g., openness and extraversion) and body dissatisfaction are important in maintaining the network in a community sample of Chinese adults. While future replication is needed, findings from this study suggest that individuals with negative self-thinking, predisposed neuroticism, and extraversion may be at risk of developing disordered eating symptoms. PUBLIC SIGNIFICANCE: The present study contributes to existing knowledge by employing a network perspective to examine the associations between disordered eating symptoms, big-five personality traits, and psychological distress in a Chinese adult community sample. The identified facets of neuroticism and extraversion and symptoms of psychological distress may be worthy of targeting in the prevention and treatment of disordered eating in the Chinese context.
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Transtornos da Alimentação e da Ingestão de Alimentos , Angústia Psicológica , Masculino , Humanos , Adulto , Personalidade , Emoções , Transtornos da Alimentação e da Ingestão de Alimentos/diagnóstico , Neuroticismo , Inventário de PersonalidadeRESUMO
Phototheranostics that concurrently integrates accurate diagnosis (e.g., fluorescence and photoacoustic (PA) imaging) and in situ therapy (e.g., photodynamic therapy (PDT) and photothermal therapy (PTT)) into one platform represents an attractive approach for accelerating personalized and precision medicine. The second near-infrared window (NIR-II, 1000-1700 nm) has attracted considerable attention from both the scientific community and clinical doctors for improved penetration depth and excellent spatial resolution. NIR-II agents with a PDT property as well as other functions are recently emerging as a powerful tool for boosting the phototheranostic outcome. In this minireview, we summarize the recent advances of photodynamic NIR-II aggregation-induced emission luminogens (AIEgens) for biomedical applications. The molecular design strategies for tuning the electronic bandgaps and photophysical energy transformation processes are discussed. We also highlight the biomedical applications, such as image-guided therapy of both subcutaneous and orthotopic tumors, and multifunctional theranostics in combination with other treatment methods, including chemotherapy and immunotherapy; and the precise treatment of both tumor and bacterial infection. This review aims to provide guidance for PDT agents with long-wavelength emissions to improve the imaging precision and treatment efficacy. We hope it will provide a comprehensive understanding about the chemical structure-photophysical property-biomedical application relationship of NIR-II luminogens.
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Nanopartículas , Neoplasias , Fotoquimioterapia , Fluorescência , Humanos , Nanopartículas/química , Neoplasias/diagnóstico por imagem , Neoplasias/tratamento farmacológico , Fotoquimioterapia/métodos , Medicina de Precisão , Nanomedicina Teranóstica/métodosRESUMO
PURPOSE: This paper aimed to explore the association between the patterns of perceived parenting styles and adolescents' night eating syndrome symptoms and correlates, including sleep quality, weight status, loss of control over eating, and psychological distress. METHODS: A sample of 455 Chinese adolescents (54.5% females, aged 12-15 years) were included in the current study. Latent Profile Analysis (LPA) was adopted to examine the patterns of perceived parenting styles. The three-step approach was used to explore the differences in night eating syndrome symptoms and correlates between different profiles. RESULTS: A four-profile solution was found to fit the data best, and the four profiles were labeled as positive parenting, negative parenting, highly engaged parenting, and lowly engaged parenting. Subsequent analyses showed that adolescents across profiles exhibited significant differences in night eating syndrome symptoms and correlates. Specifically, adolescents in the positive parenting profile generally had the lowest scores in night eating and its correlates, while those in the negative parenting group reported the highest scores in night eating and its correlates. CONCLUSION: Using a person-centered approach (i.e., LPA), the present study identified four distinct patterns of perceived parenting styles in a sample of Chinese adolescents, with night eating and related symptomatology differing across each profile. Future interventions targeting night eating among adolescents may consider the potential influence from the patterns of perceived parenting styles to have a better intervention outcome. LEVEL OF EVIDENCE: Level V, cross-sectional descriptive study.
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Síndrome do Comer Noturno , Poder Familiar , Adolescente , Criança , China , Estudos Transversais , Feminino , Humanos , Masculino , Relações Pais-Filho , Poder Familiar/psicologia , Inquéritos e QuestionáriosRESUMO
Seed germination is a critical stage in the plant life cycle and it plays an important role in the efficiency of agricultural production. However, our knowledge of the mechanisms that regulate seed germination remains limited. In this study, we identified a novel gene, MAPK11, that encodes mitogen-activated protein kinase 11; its expression was significantly higher in seeds of tomato varieties with a low optimum germination temperature than in those with a high optimum germination temperature. In tests at 25 °C, overexpression of MAPK11 in an accession with optimum germination at 25 °C resulted in a decrease in germination, whereas RNAi of MAPK11 in an accession with optimum germination at 15 °C resulted in increased germination. Furthermore, we found that lines overexpressing MAPK11 exhibited hypersensitivity to ABA during germination. These observations were at least partially explained by the fact that MAPK11 up-regulated both NCED1 expression and ABA biosynthesis, and that it also affected ABA signaling and negatively regulated germination by influencing the phosphorylation of SnRK2.2 in vivo. In addition, we found that MAPK11 interacts with and phosphorylates SnRK1 in vivo, thereby potentially inhibiting its activation. SnRK1 interacted with ABI5 and suppressed the transcription of ABI5, thereby affecting ABA signaling and the regulation of germination. Our results demonstrate that ABA signaling in tomato is affected by a mechanism that depends on MAPK11 phosphorylating SnRKs, and this ultimately influences seed germination.
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Ácido Abscísico/metabolismo , Proteína Quinase 11 Ativada por Mitógeno/metabolismo , Solanum lycopersicum , Fatores de Transcrição de Zíper de Leucina Básica/metabolismo , Regulação da Expressão Gênica de Plantas , Germinação , Solanum lycopersicum/genética , Solanum lycopersicum/metabolismo , Proteínas de Membrana , Fosfoproteínas , Plantas Geneticamente Modificadas/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Sementes/genética , Sementes/metabolismoRESUMO
Structured light illumination is a process of 3D scanning using a digital projector to project a series of striped patterns that sweep a target surface, and based on the warping of the stripes viewed by a camera, the shape of the target can be reconstructed. In the case of scanning stripes separately in both horizontal and vertical directions, algorithms have been proposed that achieve real-time reconstruction through look-up tables; however, these look-up tables implement the inversion of one traditional lens projection matrix. In this Letter, we propose look-up tables constructed using (1) both views of a camera and a projector and (2) basic arithmetic operations rather than complex matrix operations to significantly reduce the total number of computations used to reconstruct a point cloud. Experiments show that, with the same accuracy, the proposed tables improve the computation speed by a factor of 6.66×, from 31.00 to 206.61 fps.
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OBJECTIVE: Eating disorders and obesity are commonly recognized as key public health concerns worldwide. Although rates of obesity and disordered eating have traditionally been lower in China than Western countries, these rates are on the rise. As such, interest is growing in identifying mechanisms that may address these conditions. While associations between body weight and dissatisfaction are well established, burgeoning research aims to examine how these factors are related to dietary restraint and body image inflexibility. This study aimed to explore the possible mediation effect of body dissatisfaction and body image inflexibility between body weight (body mass index) and dietary restraint. Furthermore, we explored how these relationships differed across men and women. METHODS: A sample of 1068 young adults (563 females and 505 males) in China participated in the study. Participants completed the Eating Disorder Inventory and Three-Factor Eating Questionnaire as well as the Body Image-Acceptance and Action Questionnaire. RESULTS: Results showed that: (1) body dissatisfaction and body image inflexibility fully mediated the relationship between body mass index and dietary restraint; (2) this model fit both genders, although differences were found in the regression coefficients between the mediation model for men and women. CONCLUSION: These findings support body image dissatisfaction and inflexibility as mediators of the relationship between body weight and dietary restraint, highlighting these as potential mechanisms for treatment. LEVEL OF EVIDENCE: Level III, case-control analytic study.
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Insatisfação Corporal , Transtornos da Alimentação e da Ingestão de Alimentos , Imagem Corporal , Índice de Massa Corporal , Peso Corporal , China , Dieta , Feminino , Humanos , Masculino , Adulto JovemRESUMO
Multiple-phase-shifted structured light illumination achieves high-accuracy 3D reconstructions of static objects, while typically it can't achieve real-time phase computation. In this paper, we propose to compute modulations and phases of multiple scans in real time by using divide-and-conquer solutions. First, we categorize total N = KM images into M groups and each group contains K phase equally shifted images; second, we compute the phase of each group; and finally, we obtain the final phase by averaging all the separately computed phases. When K = 3, 4 or 6, we can use integer-valued intensities of images as inputs and build one or M look-up tables storing real-valued phases computed by using arctangent function. Thus, with addition and/or subtraction operations computing indices of the tables, we can directly access the pre-computed phases and avoid time-consuming arctangent computation. Compared with K-step phase measuring profilometry repeated for M times, the proposed is robust to nonlinear distortion of structured light systems. Experiments show that, first, the proposed is of the same accuracy level as the traditional algorithm, and secondly, with employing one core of a central processing unit, compared with the classical 12-step phase measuring profilometry algorithm, for K = 4 and M = 3, the proposed improves phase computation by a factor of 6 ×.
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Structured light illumination, scanning along both horizontal and vertical directions, achieves more robust accuracy. By introducing the constraint of epipolar geometry, we previously proposed real-time 3D reconstruction using lookup tables; however, we only knew these offline derived tables were the combinations of the elements in calibration matrices of a camera and a projector, and suffered from long-time computation. In this Letter, by parameterizing the line perspectively mapping a 3D world coordinate into the camera and projector spaces, we propose to extend the epipolar analysis by defining phase and optical poles. Thus, we can geometrically address these parameters via analytic closed-form equations, with which we can (1) directly derive lookup tables in real time from the calibration matrices and (2) optimally reduce the number of tables from 11 to 5 to save much more memory space while further accelerating the processing rate. Experiments show that with the same level of accuracy, we significantly reduce the time to compute the lookup tables from more than 20 min to 20 ms, and increase the speed of computing point clouds from approximately 320 to 492 fps.
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BACKGROUND: Nocturnal enuresis (NE) has a negative impact on children's health and imposes a long-term burden on families. With economic development and cultural improvements, parents and medical professionals pay more attention to NE. The aim of this study was to investigate the prevalence and risk factors of NE among children ages 5-12 years in Xi'an, China. METHODS: A stratified cluster sampling method was used to conduct a cross-sectional study of NE in 10 kindergartens and 20 primary schools in Xi'an. We used univariate analysis to compare the prevalences of characteristics such as gender, duration of disposable diaper (DD) use, toilet training onset time, daily living habits, academic performance, and family history of NE. Logistic regression analysis was used to calculate odds ratio and to determine risk factors of NE. RESULTS: The study included 6568 children ages 5-12 years, of which 262 (3.99%) had NE. The prevalence rates of NE decreased with age, with the highest prevalence at age 5 (9.09% for boys; 6.03% for girls). However, the prevalence increased with duration of DD use. Children experienced more NE if they never accepted toilet training (7.83%) or if they drank sugary beverages during the day (5.36%). Sleep disorders, sweets intake, drinking low amounts of plain water during the day, and family history of NE, were statistically associated with NE. CONCLUSION: NE was closely associated with a family history of NE, being male, long-term use of DD, delayed toilet training, drinking sugary beverages and/or consuming little plain water, and sleep disorders. A supportive parental attitude towards NE and timely medical treatment can improve the quality of life of enuretic children.
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Enurese Noturna , Criança , Pré-Escolar , China/epidemiologia , Estudos Transversais , Feminino , Humanos , Masculino , Enurese Noturna/epidemiologia , Enurese Noturna/etiologia , Prevalência , Qualidade de Vida , Fatores de RiscoRESUMO
For temporal phase unwrapping in phase measuring profilometry, it has recently been reported that two phases with co-prime frequencies can be absolutely unwrapped using a look-up table; however, frequency selection and table construction has been performed manually without optimization. In this paper, a universal phase unwrapping method is proposed to unwrap phase flexibly and automatically by using geometric analysis, and thus we can programmatically build a one-dimensional or two-dimensional look-up table for arbitrary two co-prime frequencies to correctly unwrap phases in real time. Moreover, a phase error model related to the defocus effect is derived to figure out an optimal reference frequency co-prime to the principal frequency. Experimental results verify the correctness and computational efficiency of the proposed method.
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By scanning static, not moving, objects along both the horizontal and vertical axes instead of one, structured light illumination achieves more accurate and robust 3D surface reconstructions but with greater latency on computing 3D point clouds. If scanning is performed along only one axis, it has been reported that look-up tables, manually derived from the calibration matrices of a camera and a projector, can significantly help to speed up computation; however, it has been nearly impossible to manually derive similar look-up tables for phases scanned along two axes. In this Letter, we bridge this divide by introducing the constraint of epipolar geometry to automatically compute look-up tables and thus, significantly speed up computing 3D point clouds with only basic arithmetic operations rather than time-consuming matrix computations. Experimental results show that the proposed method, using only single-thread CPU computing, reduces process latency by an order of magnitude.
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Autotaxin (ATX), which is highly expressed and secreted by adipocytes, functions as the key enzyme to generate lysophosphatidic acid (LPA) from lysophosphatidylcholine. Adipose tissue is the main source of circulating ATX that modulates plasma LPA levels. Upregulation of ATX expression in obese patients and mice is closely related with insulin resistance and impaired glucose tolerance. However, the mechanism of ATX expression in adipocytes remains largely unknown. In this study, we found that glycoprotein 130 (gp130)-mediated Janus kinase (JAK)-signal transducer and activator of transcription 3 (STAT3) activation was required for abundant ATX expression in adipocytes. Through gp130, the interleukin 6 (IL-6) family cytokines, such as IL-6, leukemia inhibitory factor, cardiotrophin-1, and ciliary neurotrophic factor, upregulated ATX expression in adipocytes. ATX contributes to the induction of insulin resistance and lipolysis in IL-6-stimulated adipocytes. Oral administration of gp130 inhibitor SC144 suppressed ATX expression in adipose tissue, decreased plasma ATX, LPA, and FFA levels, and significantly improved insulin sensitivity and glucose tolerance in high-fat diet-fed obese mice. In summary, our results indicate that the activation of gp130-JAK-STAT3 pathway by IL-6 family cytokines has an important role in regulating ATX expression in adipocytes and that gp130 is a promising target in the management of obesity-associated glucose metabolic diseases.
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Adipócitos/efeitos dos fármacos , Receptor gp130 de Citocina/metabolismo , Regulação para Baixo/efeitos dos fármacos , Resistência à Insulina , Obesidade/patologia , Diester Fosfórico Hidrolases/metabolismo , Transdução de Sinais/efeitos dos fármacos , Células 3T3-L1 , Adipócitos/metabolismo , Adipócitos/patologia , Animais , Dieta/efeitos adversos , Ácidos Graxos não Esterificados/sangue , Glucose/metabolismo , Hidrazinas/farmacologia , Interleucina-6/metabolismo , Isoxazóis/farmacologia , Janus Quinases/metabolismo , Lipólise/efeitos dos fármacos , Lisofosfolipídeos/sangue , Camundongos , Obesidade/induzido quimicamente , Obesidade/metabolismo , Propionatos/farmacologia , Quinoxalinas/farmacologia , Fator de Transcrição STAT3/metabolismoRESUMO
OBJECTIVE: To identify the candidate chromosomal region for congenital preauricular fistula (CPF) through analysis of an affected Chinese family. METHODS: Conventional linkage analysis using short tandem repeats (STR) markers was performed to investigate three chromosomal regions 8q11.1-q13.3, 1q32-q34.3 and 14q31.1-q31.3. RESULTS: None of 16 STRs could attain a LOD score of more than -2.0 (theta=0). Therefore, the three regions were all excluded as the candidate region for the disease. CONCLUSION: CPF features high genetic heterogeneity. The family may have a causative gene elsewhere. Whole-genome-based study is needed to identify its genetic etiology.
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Cromossomos Humanos Par 14/genética , Cromossomos Humanos Par 1/genética , Cromossomos Humanos Par 8/genética , Anormalidades Craniofaciais/genética , Adulto , Povo Asiático/genética , China , Feminino , Humanos , Escore Lod , Masculino , Repetições de Microssatélites , LinhagemRESUMO
Previous research identified four patterns of negative emotional eating in American and Chinese university students and proposed future directions (e.g., exploring potential differences in emotion regulation across patterns and replicating the patterns in a general, non-student population). Furthermore, prior research has not explored group differences in muscularity-oriented eating disorder symptomatology or psychosocial impairment. Therefore, the present study addressed these gaps in a sample of general Chinese adults, further testing group differences in typical and muscularity-oriented eating disorder symptomatology, psychosocial impairment, and emotion regulation difficulties across patterns of negative emotional eating. A total of 600 Chinese adults were recruited. Latent class analysis (LCA) was used. Results replicated the four patterns of negative emotional eating in previous research, including non-emotional eating (non-EE), emotional over- and under-eating (EOE-EUE), emotional over-eating (EOE), and emotional under-eating (EUE). Significant class differences were identified in eating disorder symptomatology, psychosocial impairment, and emotion regulation difficulties. Specifically, individuals with EOE and EOE-EUE patterns exhibited higher eating disorder symptomatology, higher psychosocial impairment, and more emotion regulation difficulties than those with non-EE and EUE patterns. Therefore, these two classes (i.e., EOE and EOE-EUE), especially the poorly researched EOE-EUE group, should be further examined to elucidate research and clinical applications. Furthermore, findings underscore the role of emotion regulation difficulties in further describing the differences across these negative emotional eating patterns, which can be considered in future interventions for reducing negative emotional eating.
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Rheumatoid arthritis (RA) severely lowers the life quality by progressively destructing joint functions and eventually causing permanent disability, representing a pressing public health concern. The pathogenesis of RA includes the excessive production of proinflammatory cytokines and harmful oxygen-derived free radicals, such as nitric oxide (NO), which constitute vital targets for precise diagnosis and effective treatment of RA. In this study, we introduce an advanced nanoagent that integrates the RA microenvironment-activatable photoacoustic (PA) imaging with multitarget synergistic treatment for RA. A highly sensitive organic probe with NO-tunable energy transformation and molecular geometry is developed, which enables strong near-infrared absorption with a turn-on PA signal, and the active intramolecular motion could further boost PA conversion. The probe is coassembled with an inflammation-responsive prodrug to construct the theranostic nanoagent, on which a macrophage-derived cell membrane with natural tropism to the inflammatory sites is camouflaged to improve the targeting ability to inflamed joints. The nanoagent could not only sensitively detect RA and differentiate the severity but also efficiently alleviate RA symptoms and improve joint function. The combination of activatable probe-mediated NO scavenging and on-demand activation of anti-inflammatory prodrug significantly inhibits the proinflammatory factors and promotes macrophage repolarization from M1 to M2 phenotype. This meticulously designed nanoagent ingeniously integrates RA-specific PA molecular imaging with synergistic multitarget therapy, rendering tremendous promise for precise intervention of RA-related diseases.
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Artrite Reumatoide , Pró-Fármacos , Humanos , Artrite Reumatoide/diagnóstico por imagem , Artrite Reumatoide/tratamento farmacológico , Artrite Reumatoide/metabolismo , Inflamação/diagnóstico por imagem , Inflamação/tratamento farmacológico , Citocinas , Imagem Molecular , Pró-Fármacos/uso terapêuticoRESUMO
Body dissatisfaction is a global public health concern. Self-injurious thoughts and behaviours (SITB), including suicidal ideation, suicide attempts and non-suicidal self-injury (NSSI), have been documented as potentially significant correlates of body dissatisfaction. However, prior findings regarding associations between body dissatisfaction and SITB have been somewhat inconsistent. Therefore, this meta-analysis was conducted to determine the nature and strength of such associations in both clinical and non-clinical samples. A literature search identified 83 relevant articles and extracted 234 effect sizes. Using a three-level random-effects model, mean effect sizes (r values) for relationships between body dissatisfaction and suicidal ideation, suicide attempts, and NSSI in clinical samples were 0.29 (95% CI, 0.22-0.37), 0.16 (95% CI, 0.13-0.20) and 0.26 (95% CI, 0.19-0.34), respectively. In non-clinical samples, these values were 0.22 (95% CI, 0.16-0.28), 0.24 (95% CI, 0.17-0.30) and 0.22 (95% CI, 0.15-0.29), respectively. Several study features (e.g., participant age, geographic region and instrument validity) emerged as significant moderators. This meta-analysis provides robust support for body dissatisfaction as a significant correlate of SITB across clinical and non-clinical samples in addition to identifying study characteristics that contribute to effect size variability. Implications are discussed for SITB research, prevention and intervention.
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Insatisfação Corporal , Comportamento Autodestrutivo , Ideação Suicida , Tentativa de Suicídio , Humanos , Comportamento Autodestrutivo/psicologia , Insatisfação Corporal/psicologiaRESUMO
The current study translated the Appearance Schemas Inventory-Revised (ASI-R) into Chinese (Mandarin) and examined its psychometric properties in Chinese adult women and men. Sample 1 included 400 women and 400 men to examine the factor structure of the ASI-R with exploratory factor analysis (EFA). Sample 2 involved 300 women and 300 men, and the EFA-derived factor structures in Sample 1 of the ASI-R were examined with exploratory structural equation modeling (ESEM), bifactor ESEM (B-ESEM), and bifactor ESEM with correlated uniqueness for negatively worded items (B-ESEM-CU) for both women and men. Results of the EFA identified a 4-factor model in women and a 2-factor model in men. The B-ESEM-CU consistently showed the best model fit. In the B-ESEM-CU, the general factor was well-defined, but the specific factors were not, supporting the use of the global factor to conceptualize the ASI-R for Chinese women and men. Evidence of adequate internal consistency, test-retest reliability, and construct validity of the global factor of the ASI-R was suggested in both women and men. Findings suggest the ASI-R is a useful instrument to measure body image investment in Chinese women and men, specifically using the B-ESEM-CU to understand the dimensionality of the ASI-R.
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Povo Asiático , Imagem Corporal , Adulto , Masculino , Humanos , Feminino , Imagem Corporal/psicologia , Reprodutibilidade dos Testes , Inquéritos e Questionários , PsicometriaRESUMO
Advanced photosensitizers for high-performance fluorescence imaging-guided photothermal therapy demand excellent near-infrared (NIR) brightness [molar absorption coefficient (ε) × quantum yield (QY)] and exceptional photothermal performance [ε × photothermal conversion efficiency (PCE)]. However, integrating high brightness and potent photothermal performance within a single molecule faces a formidable challenge. This article proposes a method to address this issue by preparing J-aggregate nanoparticles (NPs) using molecules with high ε. J-aggregates effectively improve QY and induce molecular emission redshift, while high ε molecules play a crucial role in improving the brightness and photothermal performance. By optimizing the molecular structure based on the pyrrolopyrrole cyanine (PPCy), precise control over the QY and PCE of PPCy J-aggregates is achieved. Ultimately, PDDO NPs exhibiting superior brightness (ε × QY = 3.32 × 104 M-1 cm-1) and photothermal performance (ε × PCE = 1.21 × 105 M-1 cm-1) are identified as high-performance photosensitizers. Notably, each parameter represents one of the highest levels among the reported fluorescence or photothermal probes to date. The in vivo studies demonstrate that PDDO NPs possess exceptional NIR imaging capabilities and remarkable photothermal tumor inhibition rates. This study provides innovative insights into the development of high-performance multifunctional photosensitizers.