Statistical Benchmarking of Scalable Photonic Quantum Systems.

Targeting at the realization of scalable photonic quantum technologies, the generation of many photons, their propagation in large optical networks, and a subsequent detection and analysis of sophisticated quantum correlations are essential for the understanding of macroscopic quantum systems. In this experimental contribution, we explore the joint operation of all mentioned ingredients. We benchmark our time-multiplexing framework that includes a high-performance source of multiphoton states and a large multiplexing network, together with unique detectors with high photon-number resolution, readily available for distributing quantum light and measuring complex quantum correlations. Using an adaptive approach that employs flexible time bins, rather than static ones, we successfully verify high-order nonclassical correlations of many photons distributed over many modes. By exploiting the symmetry of our system and using powerful analysis tools, we can analyze correlations that would be inaccessible by classical means otherwise. In particular, we produce on the order of ten photons and distribute them over 64 modes. Nonclassicality is verified with correlation functions up to the 128th order and statistical significances of up to 20 standard deviations.

Quantum Correlations beyond Entanglement and Discord.

Dissimilar notions of quantum correlations have been established, each being motivated through particular applications in quantum information science and each competing for being recognized as the most relevant measure of quantumness. In this contribution, we experimentally realize a form of quantum correlation that exists even in the absence of entanglement and discord. We certify the presence of such quantum correlations via negativities in the regularized two-mode Glauber-Sudarshan function. Our data show compatibility with an incoherent mixture of orthonormal photon-number states, ruling out quantum coherence and other kinds of quantum resources. By construction, the quantumness of our state is robust against dephasing, thus requiring fewer experimental resources to ensure stability. In addition, we theoretically show how multimode entanglement can be activated based on the generated, nonentangled state. Therefore, we implement a robust kind of nonclassical photon-photon correlated state with useful applications in quantum information processing.

Detector-Agnostic Phase-Space Distributions.

The representation of quantum states via phase-space functions constitutes an intuitive technique to characterize light. However, the reconstruction of such distributions is challenging as it demands specific types of detectors and detailed models thereof to account for their particular properties and imperfections. To overcome these obstacles, we derive and implement a measurement scheme that enables a reconstruction of phase-space distributions for arbitrary states whose functionality does not depend on the knowledge of the detectors, thus defining the notion of detector-agnostic phase-space distributions. Our theory presents a generalization of well-known phase-space quasiprobability distributions, such as the Wigner function. We implement our measurement protocol, using state-of-the-art transition-edge sensors without performing a detector characterization. Based on our approach, we reveal the characteristic features of heralded single- and two-photon states in phase space and certify their nonclassicality with high statistical significance.

Experimental Reconstruction of Entanglement Quasiprobabilities.

We report on the first experimental reconstruction of an entanglement quasiprobability. In contrast to related techniques, the negativities in our distributions are a necessary and sufficient identifier of separability and entanglement and enable a full characterization of the quantum state. A reconstruction algorithm is developed, a polarization Bell state is prepared, and its entanglement is certified based on the reconstructed entanglement quasiprobabilities, with a high significance and without correcting for imperfections.

Incomplete Detection of Nonclassical Phase-Space Distributions.

We implement the direct sampling of negative phase-space functions via unbalanced homodyne measurement using click-counting detectors. The negativities significantly certify nonclassical light in the high-loss regime using a small number of detectors which cannot resolve individual photons. We apply our method to heralded single-photon states and experimentally demonstrate the most significant certification of nonclassicality for only two detection bins. By contrast, the frequently applied Wigner function fails to directly indicate such quantum characteristics for the quantum efficiencies present in our setup without applying additional reconstruction algorithms. Therefore, we realize a robust and reliable approach to characterize nonclassical light in phase space under realistic conditions.

Separable and Inseparable Quantum Trajectories.

The dynamical behavior of interacting systems plays a fundamental role for determining quantum correlations, such as entanglement. In this Letter, we describe temporal quantum effects of the inseparable evolution of composite quantum states by comparing the trajectories to their classically correlated counterparts. For this reason, we introduce equations of motions describing the separable propagation of any interacting quantum system, which are derived by requiring separability for all times. The resulting Schrödinger-type equations allow for comparing the trajectories in a separable configuration with the actual behavior of the system and, thereby, identifying inseparable and time-dependent quantum properties. As an example, we study bipartite discrete- and continuous-variable interacting systems. The generalization of our developed technique to multipartite scenarios is also provided.

Conditional Hybrid Nonclassicality.

We derive and implement a general method to characterize the nonclassicality in compound discrete- and continuous-variable systems. For this purpose, we introduce the operational notion of conditional hybrid nonclassicality which relates to the ability to produce a nonclassical continuous-variable state by projecting onto a general superposition of discrete-variable subsystem. We discuss the importance of this form of quantumness in connection with interfaces for quantum communication. To verify the conditional hybrid nonclassicality, a matrix version of a nonclassicality quasiprobability is derived and its sampling approach is formulated. We experimentally generate an entangled, hybrid Schrödinger cat state, using a coherent photon-addition process acting on two temporal modes, and we directly sample its nonclassicality quasiprobability matrix. The introduced conditional quantum effects are certified with high statistical significance.

Detector-Independent Verification of Quantum Light.

We introduce a method for the verification of nonclassical light which is independent of the complex interaction between the generated light and the material of the detectors. This is accomplished by means of a multiplexing arrangement. Its theoretical description yields that the coincidence statistics of this measurement layout is a mixture of multinomial distributions for any classical light field and any type of detector. This allows us to formulate bounds on the statistical properties of classical states. We apply our directly accessible method to heralded multiphoton states which are detected with a single multiplexing step only and two detectors, which are in our work superconducting transition-edge sensors. The nonclassicality of the generated light is verified and characterized through the violation of the classical bounds without the need for characterizing the used detectors.

Multipartite Entanglement of a Two-Separable State.

We consider a six-partite, continuous-variable quantum state that we have effectively generated by the parametric down-conversion of a femtosecond frequency comb. We show that, though this state is two-separable, i.e., it does not exhibit "genuine entanglement," it is undoubtedly multipartite entangled. The consideration of not only the entanglement of individual mode decompositions, but also of combinations of those, solves the puzzle and exemplifies the importance of studying different categories of multipartite entanglement.

Quantum Correlations from the Conditional Statistics of Incomplete Data.

We study, in theory and experiment, the quantum properties of correlated light fields measured with click-counting detectors providing incomplete information on the photon statistics. We establish a correlation parameter for the conditional statistics, and we derive the corresponding nonclassicality criteria for detecting conditional quantum correlations. Classical bounds for Pearson's correlation parameter are formulated that allow us, once they are violated, to determine nonclassical correlations via the joint statistics. On the one hand, we demonstrate nonclassical correlations in terms of the joint click statistics of light produced by a parametric down-conversion source. On the other hand, we verify quantum correlations of a heralded, split single-photon state via the conditional click statistics together with a generalization to higher-order moments. We discuss the performance of the presented nonclassicality criteria to successfully discern joint and conditional quantum correlations. Remarkably, our results are obtained without making any assumptions on the response function, quantum efficiency, and dark-count rate of photodetectors.

Uncovering Quantum Correlations with Time-Multiplexed Click Detection.

We report on the implementation of a time-multiplexed click detection scheme to probe quantum correlations between different spatial optical modes. We demonstrate that such measurement setups can uncover nonclassical correlations in multimode light fields even if the single mode reductions are purely classical. The nonclassical character of correlated photon pairs, generated by a parametric down-conversion, is immediately measurable employing the theory of click counting instead of low-intensity approximations with photoelectric detection models. The analysis is based on second- and higher-order moments, which are directly retrieved from the measured click statistics, for relatively high mean photon numbers. No data postprocessing is required to demonstrate the effects of interest with high significance, despite low efficiencies and experimental imperfections. Our approach shows that such novel detection schemes are a reliable and robust way to characterize quantum-correlated light fields for practical applications in quantum communications.

Full multipartite entanglement of frequency-comb Gaussian states.

An analysis is conducted of the multipartite entanglement for Gaussian states generated by the parametric down-conversion of a femtosecond frequency comb. Using a recently introduced method for constructing optimal entanglement criteria, a family of tests is formulated for mode decompositions that extends beyond the traditional bipartition analyses. A numerical optimization over this family is performed to achieve maximal significance of entanglement verification. For experimentally prepared 4-, 6-, and 10-mode states, full entanglement is certified for all of the 14, 202, and 115 974 possible nontrivial partitions, respectively.

Structural quantification of entanglement.

We introduce an approach which allows a full structural and quantitative analysis of multipartite entanglement. The sets of states with different structures are convex and nested. Hence, they can be distinguished from each other using appropriate measurable witnesses. We derive equations for the construction of optimal witnesses and discuss general properties arising from our approach. As an example, we formulate witnesses for a 4-cluster state and perform a full quantitative analysis of the entanglement structure in the presence of noise and losses. The strength of the method in multimode continuous variable systems is also demonstrated for a dephased Greenberger-Horne-Zeilinger-type state.

Multipartite entanglement witnesses.

We derive a set of algebraic equations, the so-called multipartite separability eigenvalue equations. Based on their solutions, we introduce a universal method for the construction of multipartite entanglement witnesses. We witness multipartite entanglement of 10(3) coupled quantum oscillators, by solving our basic equations analytically. This clearly demonstrates the feasibility of our method for studying ultrahigh orders of multipartite entanglement in complex quantum systems.

Sub-binomial light.

The click statistics from on-off detector systems is quite different from the counting statistics of the more traditional detectors. This necessitates the introduction of new parameters to characterize the nonclassicality of fields from measurements using on-off detectors. To properly replace the Mandel Q(M) parameter, we introduce a parameter Q(B). A negative value represents a sub-binomial statistics. This is possible only for quantum fields, even for super-Poisson light. It eliminates the problems encountered in discerning nonclassicality using Mandel's Q(M) for on-off data.

A comparative evaluation of ablations produced by high-frequency coagulation-, argon plasma coagulation-, and cryotherapy devices in porcine liver.

INTRODUCTION: Hepatic resection is the only curative treatment option for primary or metastatic malignancies of the liver. Although R1 resections can also lead to prolonged survival, the main surgical goal is complete tumor resection (R0). To achieve this, additional treatment of the resection margin with ablation devices is discussed. Using a porcine in vivo model, we therefore analyzed the effect of different ablation devices on depth and completeness of hepatic parenchymal cell destruction. METHODS: Swabian-Hall strain pigs underwent ablation on the surface of the right, middle, or left liver lobe using seven different types of high-frequency (HF)-, cryotherapy (Cryo)-, or argon plasma coagulation (APC) devices. Penetration depth and volume were analyzed from histological sections. Severity of parenchymal cell destruction was assessed by a histomorphological score. RESULTS: The greatest penetration depth was achieved with Cryo (10.4 ± 1.7 mm), whereas HF and APC exhibited a smaller penetration depth. However, HF and APC compared to Cryo achieved complete destruction of the intralobular architecture and hepatocellular morphology depending on the application time and the adjusted power. CONCLUSION: HF, APC, and Cryo applied to the liver surface induce different parenchymal penetration depth and cell destruction. HF and APC are considered to be standard surgical instruments and therefore recommended as standard treatment, whereas Cryo may be used only if particularly deep penetration is required.

Hepatic arterial infusion with tumor necrosis factor-α induces early hepatic hyperperfusion.

BACKGROUND: Hepatic arterial infusion (HAI) has been developed for high-dose regional chemotherapy of unresectable liver metastases or primary liver malignancies. While it is well known that high concentrations of tumor necrosis factor (TNF)-α damage tumor blood perfusion, there is no information on whether autochthonous liver perfusion is affected by HAI with TNF-α. Therefore, we investigated the effects of HAI with TNF-α on hepatic macro- and microvascular perfusion. METHODS: Swabian Hall pigs were randomized into three groups. HAI was performed with either 20 or 40 µg/kg body weight TNF-α (n = 6 each group). Saline-treated animals served as controls (n = 6). Analyses during a 2-hour post-HAI observation period included systemic hemodynamics, portal venous and hepatic arterial blood flow, portal venous pressure, and the blood flow in the hepatic microcirculation. RESULTS: HAI with TNF-α caused a slight decrease of mean arterial blood pressure (p < 0.001), which was compensated by a moderate increase of heart rate (p < 0.001). No further systemic side effects of TNF-α were observed. HAI with TNF-α further caused a slight but not significant decrease of portal venous blood flow (p = 0.737) in both experimental groups, paralleled by an increase of hepatic arterial blood flow (p = 0.023, 20 µg/kg; p = 0.034, 40 µg/kg) resulting in an overall hepatic hyperperfusion. The hepatic hyperperfusion after HAI with 20 µg/kg TNF-α was more pronounced and associated with a 40% decrease of the blood flow in the hepatic microcirculation (p = 0.009). HAI with 40 µg/kg TNF-α was only associated with a temporary and moderate total hepatic hyperperfusion and did not affect the blood flow in the hepatic microcirculation. CONCLUSION: HAI with TNF-α causes a decrease of portal venous flow; however, this is overcompensated by an increased hepatic arterial blood flow, resulting in a total hepatic hyperperfusion. Moderate total hepatic hyperperfusion does not affect the blood flow in the hepatic microcirculation, while a persistent and more pronounced hyperperfusion may cause hepatic microcirculatory disturbances.

[Intraoperative fluorescence-guided perfusion assessment using indocyanine green-Increased safety in gastrointestinal anastomoses?] / Intraoperative fluoreszenzgestützte Perfusionsmessung mit Indocyaningrün ­ erhöhte Sicherheit bei gastrointestinalen Anastomosen?

Insufficiency of gastrointestinal anastomoses represents a relevant risk of morbidity and mortality for affected patients. The perfusion quality of the ends of the intestine is the decisive parameter for ensuring sufficient healing of an anastomosis. Intraoperative fluorescence-guided perfusion assessment with indocyanine green is increasingly being used in modern visceral surgery to evaluate tissue perfusion prior to the fashioning of gastrointestinal anastomoses. This technique provides the possibility to distinguish between adequately and inadequately perfused tissue in order to place the anastomosis in the region with the best possible perfusion. Thus, surgeons have a measuring instrument that enables an objective assessment of the perfusion quality of the tissue to be undertaken in addition to a purely subjective macroscopic visual assessment, in order to achieve a better functional result for the patients. Currently, however, the value of this technique has not yet been conclusively clarified. The aim of this review article is to characterize the benefits of intraoperative fluorescence-guided perfusion assessment and to classify it with respect to its significance for routine clinical practice.

Laser Doppler flowmetry of hepatic microcirculation during Pringle's maneuver: determination of spatial and temporal liver tissue perfusion heterogeneity.

BACKGROUND: Laser Doppler flowmetry (LDF) is frequently used for non-invasive microvascular perfusion measurements. The aim of the present study was to analyze liver blood flow heterogeneity in detail using LDF devices under normal and low-flow conditions. MATERIALS AND METHODS: In 5 anesthetized and laparotomized Suabian-Hall strain pigs, systemic hemodynamics and hepatic arterial/portal venous blood flow were constantly recorded. Hepatic microcirculation was assessed by 2 different LDF devices, analyzing microvascular flow and velocity before, during and after inducing a Pringle's maneuver for hepatic inflow occlusion. Offline data analysis comprised differentiation between the two LDF devices used as well as calculation of temporal and spatial heterogeneity of liver perfusion. RESULTS: Pringle's maneuver induced complete inflow occlusion, confirmed by hepatic arterial/portal venous blood flow measurement. Laser Doppler signals showed a significant decrease during Pringle's maneuver. Spatial heterogeneity of flow and velocity increased more than temporal heterogeneity during Pringle's maneuver. CONCLUSION: Both LDF devices proved suitable for assessing hepatic microvascular perfusion during normal perfusion and low-flow conditions. Reduced microvascular perfusion induces a significant increase in temporal and spatial perfusion heterogeneity. In particular, the pronounced spatial heterogeneity requires measurements at different places when assessing hepatic microcirculation by LDF during impaired perfusion conditions.

The regulatory complex of Drosophila melanogaster 26S proteasomes. Subunit composition and localization of a deubiquitylating enzyme.

Drosophila melanogaster embryos are a source for homogeneous and stable 26S proteasomes suitable for structural studies. For biochemical characterization, purified 26S proteasomes were resolved by two-dimensional (2D) gel electrophoresis and subunits composing the regulatory complex (RC) were identified by amino acid sequencing and immunoblotting, before corresponding cDNAs were sequenced. 17 subunits from Drosophila RCs were found to have homologues in the yeast and human RCs. An additional subunit, p37A, not yet described in RCs of other organisms, is a member of the ubiquitin COOH-terminal hydrolase family (UCH). Analysis of EM images of 26S proteasomes-UCH-inhibitor complexes allowed for the first time to localize one of the RC's specific functions, deubiquitylating activity. The masses of 26S proteasomes with either one or two attached RCs were determined by scanning transmission EM (STEM), yielding a mass of 894 kD for a single RC. This value is in good agreement with the summed masses of the 18 identified RC subunits (932 kD), indicating that the number of subunits is complete.