RESUMO
Streptomyces coelicolor A3(2) ftsI- and ftsW-null mutants produced aerial hyphae with no evidence of septation when grown on a traditional osmotically enhanced medium. This phenotype was partially suppressed when cultures were grown on media prepared without sucrose. We infer that functional FtsZ rings can form in ftsI- and ftsW-null mutants under certain growth conditions.
Assuntos
Proteínas de Bactérias/genética , Meios de Cultura/metabolismo , Proteínas de Membrana/genética , Mutação , Proteínas de Ligação às Penicilinas/genética , Streptomyces coelicolor/metabolismo , Proteínas de Bactérias/metabolismo , Divisão Celular , Proteínas de Membrana/metabolismo , Dados de Sequência Molecular , Fenótipo , Streptomyces coelicolor/citologia , Streptomyces coelicolor/genéticaRESUMO
It has recently been shown that adenoviral-mediated expression of peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1 alpha) in hepatocytes stimulates glucose-6-phosphatase catalytic subunit (G6Pase) gene expression. A combination of fusion gene, gel retardation and chromatin immunoprecipitation assays revealed that, in H4IIE cells, PGC-1 alpha mediates this stimulation through an evolutionarily conserved region of the G6Pase promoter that binds hepatocyte nuclear factor-4 alpha.