RESUMO
Triclocarban (TCC) has been used in consumer products and is a widespread contaminant in municipal wastewater treatment systems that ultimately accumulates in natural receiving water and soil. This work aims to apply an innovative hybrid moving entrapped bead activated sludge reactor (named "HyMER") that integrates entrapped TCC-degrading microbes and freely suspended activated sludge to treat TCC-contaminated wastewater. A previously isolated TCC-degrading bacterium (Pseudomonas fluorescens strain MC46, called MC46) and barium alginate entrapment were applied. The synthetic TCC-contaminated wastewater treatment (with TCC concentration of 10 mg/L) was performed using 20-cycle fed-batch reactor operation with feeding times of 12 and 24 h and cycle times of 13 and 25 h. The results indicated that the HyMER effectively reduced chemical oxygen demand by up to 80 and 95 % and TCC by up to 53 and 83 %, respectively, with feeding times of 12 and 24 h. Three TCC degradation intermediate products were found-3,4-dichloroaniline, 4-chloroaniline, and aniline. Scanning electron microscopic analysis revealed shorter cells and bacterial appendage development as cell adaptations against TCC and its intermediates. The live/dead assay indicated high survival of entrapped MC46 in toxic conditions, with up to 84 % viable cells. Based on computational fluid dynamic analysis, no entrapped cell agglomeration showed in the reactor, indicating the potential application of HyMER for real wastewater treatment. These results exhibit the feasibility of HyMER and its applicability for future toxic wastewater treatment.
Assuntos
Pseudomonas fluorescens , Purificação da Água , Esgotos/microbiologia , Águas Residuárias , Purificação da Água/métodos , Pseudomonas fluorescens/metabolismo , Reatores Biológicos , Eliminação de Resíduos Líquidos/métodosRESUMO
BACKGROUND/PURPOSE: En masse retraction was still controversy in orthodontics. The aim of this study was to investigate the effect of force directions created by different miniscrew positions and lever arm heights on maxillary central incisor movement using Finite Element (FE) simulation and a Typodont model. MATERIALS AND METHODS: A typodont model and 3-dimensional FE were used to simulate en masse anterior teeth retraction in sliding mechanics. The lever arm and the miniscrew positions were varied to change the force direction. The maxillary central incisor displacement was recorded and analyzed. RESULTS: The typodont results revealed that miniscrew vertical position and lever arm height affected the type of tooth movement. The best control in the vertical plane was achieved by a 7â¯mm lever arm height and miniscrew 9â¯mm from the archwire. When the lever arm height and miniscrew were 7â¯mm from the archwire, the tooth extruded. When the lever arm height was 9â¯mm and the miniscrew was 7 or 9â¯mm from the archwire, the tooth intruded. The FE stimulation determined that near bodily movement of the maxillary central incisor was achieved when the lever arm height and miniscrew was 9â¯mm from the archwire. The highest strain distribution in the periodontal ligament was observed at the apical third of the lateral incisor. CONCLUSION: In en masse retraction, the appropriate direction of force or the height of the miniscrew and the lever arm may enable orthodontists to maintain better control of the anterior teeth in sliding mechanics.
RESUMO
PURPOSE: The biomechanical performance of a novel engineered porous-structure implant (EPSI) with various porosities and a conventional solid-structure implant (CSSI) was investigated and compared. MATERIALS AND METHODS: The three-dimensional finite element method was applied to titanium dental implant models placed in a block of bone that included both cortical and medullary bone. Five different pore sizes and porosities of the EPSI (58% porosity [PSI-58], 62% porosity [PSI-62], 71% porosity [PSI-71], 75% porosity [PSI-75], and 79% porosity [PSI-79]), were compared with the CSSI. Equivalent von Mises (EQV) stress, strain energy density, and displacement were examined for each implant design. RESULTS: The maximum EQV stresses exhibited in cortical bone of the EPSI models were lower than those of the CSSI model. Higher EPSI porosity tended to increase the EQV stress. The EPSI appeared to share the load with the cortical bone, as evidenced by lower strain energy density in the cortical bone of EPSI models. High values for displacement were observed at the coronal part of the implant in all models. Slight differences in maximum displacement values were seen between EPSI and CSSI models. CONCLUSION: The EPSI effectively reduced the maximum EQV stress in the cortical bone and enhanced the load-sharing capacity. A significant amount of energy was absorbed by the implant instead of being transferred to the surrounding cortical bone. Varying the porosity of an implant had less effect on implant displacement.
Assuntos
Osso e Ossos , Implantes Dentários , Planejamento de Prótese Dentária/métodos , Retenção em Prótese Dentária , Análise de Elementos Finitos , Porosidade , Titânio , Fenômenos Biomecânicos , Análise do Estresse Dentário , Modelos DentáriosRESUMO
Tissue engineering scaffold is a biological substitute that aims to restore, to maintain, or to improve tissue functions. Currently available manufacturing technology, that is, additive manufacturing is essentially applied to fabricate the scaffold according to the predefined computer aided design (CAD) model. To develop scaffold CAD libraries, the polyhedrons could be used in the scaffold libraries development. In this present study, one hundred and nineteen polyhedron models were evaluated according to the established criteria. The proposed criteria included considerations on geometry, manufacturing feasibility, and mechanical strength of these polyhedrons. CAD and finite element (FE) method were employed as tools in evaluation. The result of evaluation revealed that the close-cellular scaffold included truncated octahedron, rhombicuboctahedron, and rhombitruncated cuboctahedron. In addition, the suitable polyhedrons for using as open-cellular scaffold libraries included hexahedron, truncated octahedron, truncated hexahedron, cuboctahedron, rhombicuboctahedron, and rhombitruncated cuboctahedron. However, not all pore size to beam thickness ratios (PO:BT) were good for making the open-cellular scaffold. The PO:BT ratio of each library, generating the enclosed pore inside the scaffold, was excluded to avoid the impossibility of material removal after the fabrication. The close-cellular libraries presented the constant porosity which is irrespective to the different pore sizes. The relationship between PO:BT ratio and porosity of open-cellular scaffold libraries was displayed in the form of Logistic Power function. The possibility of merging two different types of libraries to produce the composite structure was geometrically evaluated in terms of the intersection index and was mechanically evaluated by means of FE analysis to observe the stress level. The couples of polyhedrons presenting low intersection index and high stress level were excluded. Good couples for producing the reinforced scaffold were hexahedron-truncated hexahedron and cuboctahedron-rhombitruncated cuboctahedron.