RESUMO
RATIONALE: p120-catenin (p120) is an armadillo family protein that binds to the cytoplasmic domain of classical cadherins and prevents cadherin endocytosis. The role of p120 in vascular development is unknown. OBJECTIVE: The purpose of this study is to examine the role of p120 in mammalian vascular development by generating a conditionally mutant mouse lacking endothelial p120 and determining the effects of the knockout on vasculogenesis, angiogenic remodeling, and the regulation of endothelial cadherin levels. METHODS AND RESULTS: A conditional Cre/loxP gene deletion strategy was used to ablate p120 expression, using the Tie2 promoter to drive endothelial Cre recombinase expression. Mice lacking endothelial p120 died embryonically beginning at embryonic day 11.5. Major blood vessels appeared normal at embryonic day 9.5. However, both embryonic and extraembryonic vasculature of mutant animals were disorganized and displayed decreased microvascular density by embryonic day 11.5. Importantly, both vascular endothelial cadherin and N-cadherin levels were significantly reduced in vessels lacking p120. This decrease in cadherin expression was accompanied by reduced pericyte recruitment and hemorrhaging. Furthermore, p120-null cultured endothelial cells exhibited proliferation defects that could be rescued by exogenous expression of vascular endothelial cadherin. CONCLUSIONS: These findings reveal a fundamental role for p120 in regulating endothelial cadherin levels during vascular development, as well as microvascular patterning, vessel integrity, and endothelial cell proliferation. Loss of endothelial p120 results in lethality attributable to decreased microvascular density and hemorrhages.
Assuntos
Vasos Sanguíneos/embriologia , Vasos Sanguíneos/metabolismo , Cateninas/metabolismo , Células Endoteliais/metabolismo , Animais , Antígenos CD/metabolismo , Vasos Sanguíneos/patologia , Padronização Corporal , Antígenos CD8 , Caderinas/metabolismo , Cateninas/deficiência , Cateninas/genética , Proliferação de Células , Células Cultivadas , Perda do Embrião , Células Endoteliais/patologia , Idade Gestacional , Hemorragia/embriologia , Hemorragia/genética , Hemorragia/metabolismo , Imunoglobulinas , Integrases/genética , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Microvasos/embriologia , Microvasos/metabolismo , Pericitos/metabolismo , Regiões Promotoras Genéticas , Receptores Proteína Tirosina Quinases/genética , Receptor TIE-2 , delta CateninaRESUMO
Infant mental health, as concept and intervention, is poorly understood by most practitioners in education settings. Direct-service personnel often lack appropriate knowledge, training, skills, and confidence in recognizing and addressing infant mental health problems. While programs and policymakers increasingly acknowledge the need to offer infant mental health services in order to prevent or mediate poor developmental outcomes among young children, effective methods of realizing an infant mental health initiative remain elusive. This qualitative study of five early education programs examines the use of mentors trained in clinical psychology or social work to support educators in delivering infant mental health services. The perspectives of administrators, direct-service providers, and mentors help us understand what constitutes successful infant mental health mentoring in general, and videotaping in combination with reflective consultation in particular.
RESUMO
p0071 is an armadillo family protein related to both the adherens junction protein p120ctn and to the desmosomal proteins plakophilins 1-3. p0071 assembles into both adherens junctions and desmosomes, suggesting that this protein may regulate the balance between adherens junction and desmosome formation. Furthermore, this subfamily of proteins may also regulate cell functions directly influenced by intercellular junctions, including the strength of cell adhesion and the ability of cells to migrate. These possibilities were tested by expressing exogenous p0071 in A431 epithelial cells and monitoring the effects on adhesive junction assembly in comparison to other closely related armadillo family proteins. In this model system, p0071 specifically enhanced adherens junction assembly but dramatically compromised desmosome assembly, resulting in keratin filament retraction from regions of cell-cell contact. Protein interaction studies revealed that p0071 bound to the first 160 amino-terminal residues of desmoplakin and also interacted directly with plakoglobin, suggesting that p0071 may regulate desmosome assembly by controlling plakoglobin availability. Using an in vitro assay to measure the strength of cell-cell contacts, both plakophilin-1 and p120ctn were found to increase the strength of adhesion. Interestingly, p0071 expression caused no overall changes in adhesive strength, but dramatically inhibited the ability of A431 cells to close an in vitro wound. These results suggest that p120ctn/plakophilin family proteins interact with intercellular junction binding partners to differentially modulate the adhesive and migratory behavior of epithelial cells.
Assuntos
Junções Aderentes/metabolismo , Adesão Celular/fisiologia , Movimento Celular/fisiologia , Proteínas do Citoesqueleto/metabolismo , Células Epiteliais/citologia , Sítios de Ligação/fisiologia , Carcinoma de Células Escamosas , Linhagem Celular Tumoral , Proteínas do Citoesqueleto/genética , Desmoplaquinas , Desmossomos/química , Desmossomos/genética , Desmossomos/metabolismo , Células Epiteliais/metabolismo , Expressão Gênica , Humanos , Rim/citologia , Mutagênese , Placofilinas , Estrutura Terciária de Proteína , Cicatrização/fisiologia , gama CateninaRESUMO
Desmosomes are adhesive intercellular junctions prominent in the skin and heart. Loss of desmosome function is associated with severe congenital and acquired disorders characterized by tissue fragility. Pemphigus vulgaris (PV) is an autoimmune disorder in which antibodies are directed against the desmosomal adhesion molecule Dsg3, resulting in severe mucosal erosions and epidermal blistering. To define the mechanisms by which Dsg3 autoantibodies disrupt keratinocyte adhesion, the fate of PV IgG and various desmosomal components was monitored in primary human keratinocytes exposed to PV patient IgG. PV IgG initially bound to keratinocyte cell surfaces and colocalized with desmosomal markers. Within 6 h after PV IgG binding to Dsg3, electron microscopy revealed that desmosomes were dramatically disrupted and keratinocyte adhesion was severely compromised. Immunofluorescence analysis indicated that PV IgG and Dsg3 were rapidly internalized from the cell surface in a complex with plakoglobin but not desmoplakin. Dsg3 internalization was associated with retraction of keratin filaments from cell-cell borders. Furthermore, the internalized PV IgG-Dsg3 complex colocalized with markers for both endosomes and lysosomes, suggesting that Dsg3 was targeted for degradation. Consistent with this possibility, biotinylation experiments demonstrated that soluble Dsg3 cell surface pools were rapidly depleted followed by loss of detergent-insoluble Dsg3. These findings demonstrate that Dsg3 endocytosis, keratin filament retraction, and the loss of keratinocyte cell-cell adhesion are coordinated responses to PV IgG.
Assuntos
Autoanticorpos/química , Desmogleínas/química , Desmossomos/química , Pênfigo/imunologia , Biotinilação , Western Blotting , Adesão Celular , Linhagem Celular , Membrana Celular/metabolismo , Proteínas do Citoesqueleto/química , Desmogleína 3/química , Desmossomos/metabolismo , Detergentes/farmacologia , Endocitose , Humanos , Imunoglobulina G/química , Queratinócitos/metabolismo , Queratinas/química , Lisossomos/metabolismo , Microscopia Eletrônica , Microscopia de Fluorescência , Microscopia Imunoeletrônica , Modelos Biológicos , Mucosa/metabolismo , Miocárdio/metabolismo , Ligação Proteica , Pele/metabolismo , Estreptavidina/química , Estresse Mecânico , Fatores de Tempo , gama Catenina/químicaRESUMO
VE-cadherin is an endothelial-specific cadherin that plays important roles in vascular morphogenesis and growth control. To investigate the mechanisms by which endothelial cells regulate cadherin cell surface levels, a VE-cadherin mutant containing the non-adhesive interleukin-2 (IL-2) receptor extracellular domain and the VE-cadherin cytoplasmic tail (IL-2R-VE-cadcyto) was expressed in microvascular endothelial cells. Expression of the IL-2R-VE-cadcyto mutant resulted in the internalization of endogenous VE-cadherin and in a dramatic decrease in endogenous VE-cadherin levels. The internalized VE-cadherin co-localized with early endosomes, and the lysosomal inhibitor chloroquine dramatically inhibited the down-regulation of VE-cadherin in cells expressing the IL-2R-VE-cadcyto mutant. Chloroquine treatment also resulted in the accumulation of a VE-cadherin fragment lacking the beta-catenin binding domain of the VE-cadherin cytoplasmic tail. The formation of the VE-cadherin fragment could be prevented by treating endothelial cells with proteasome inhibitors. Furthermore, inhibition of the proteasome prevented VE-cadherin internalization and inhibited the disruption of endothelial intercellular junctions by the IL-2RVE-cadcyto mutant. These results provide new insights into the mechanisms of VE-cadherin processing and degradation in microvascular endothelial cells.
Assuntos
Caderinas/metabolismo , Endotélio Vascular/metabolismo , Adenoviridae/genética , Animais , Antígenos CD , Western Blotting , Células COS , Caderinas/genética , Linhagem Celular , Células Cultivadas , Cloroquina/farmacologia , Cisteína Endopeptidases , Endossomos/metabolismo , Imunofluorescência , Deleção de Genes , Expressão Gênica , Vetores Genéticos , Humanos , Junções Intercelulares/efeitos dos fármacos , Rim , Lisossomos/efeitos dos fármacos , Lisossomos/metabolismo , Masculino , Microcirculação , Complexos Multienzimáticos/antagonistas & inibidores , Mutagênese , Complexo de Endopeptidases do Proteassoma , Receptores de Interleucina-2/genética , Proteínas Recombinantes de Fusão , Proteínas Recombinantes , Pele/irrigação sanguínea , TransfecçãoRESUMO
VE-cadherin is an endothelial-specific cadherin that plays a central role in vascular barrier function and angiogenesis. The cytoplasmic domain of VE-cadherin is linked to the cytoskeleton through interactions with the armadillo family proteins beta-catenin and plakoglobin. Growing evidence indicates that beta-catenin and plakoglobin play important roles in epithelial growth and morphogenesis. To test the role of these proteins in vascular cells, a replication-deficient retroviral system was used to express intercellular junction proteins and mutants in the human dermal microvascular endothelial cell line (HMEC-1). A mutant VE-cadherin lacking an adhesive extracellular domain disrupted endothelial barrier function and inhibited endothelial growth. In contrast, expression of exogenous plakoglobin or metabolically stable mutants of beta-catenin stimulated HMEC-1 cell growth, which suggests that the beta-catenin signaling pathway was active in HMEC-1 cells. This possibility was supported by the finding that a dominant-negative mutant of the transcription factor TCF-4, designed to inhibit beta-catenin signaling, also inhibited HMEC-1 cell growth. These observations suggest that intercellular junction proteins function as components of an adhesion and signaling system that regulates vascular barrier function and growth.