Association of IL-6, IL-10 and TGF-ß1 gene polymorphisms with brucellosis: A systematic review with meta-analysis.

BACKGROUND: Brucellosis is one of the major public health problems worldwide. Several current studies have provided data that polymorphisms in the interleukin-6 (IL-6), interleukin-10 (IL-10) and transforming growth factor beta1(TGF-ß1) gene were associated with the susceptibility to human brucellosis, but the results remain inconsistent. OBJECTIVES: The aim of present study was to investigate the relationship between IL-6 (-174 G/C), IL-10 (-1082 A/G, -819C/T) and TGF-ß1 (codon 10, codon 25) gene polymorphisms and brucellosis. METHODS: We performed a comprehensive search of the PubMed, EMBASE, Web of Science, OVID-EBMR, and the Cochrane Library up to Oct. 30, 2018. The search was designed using the following key words: "brucellosis" or" "brucella melitensis", "IL-10" or "interleukin10" or "interleukin-10", "IL-6" or "interleukin6" or "interleukin-6", "TGF-ß1" or "TGF-beta1" or "transforming growth factor ß1", "polymorphism" and "single nucleotide polymorphism (SNP)". Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to measure the strength of association between TGF-ß1, IL-10 and IL-6 polymorphisms and brucellosis risk. All the statistical analyses were conducted by Review manager 5.3 software. RESULTS: A total of 8 studies involving 1308 cases and 902 controls met the inclusion criteria for IL-6, IL-10, TGF-ß1 polymorphisms and brucellosis risk. There was a slightly trend of increasing risk of brucellosis in individuals with the G allele compared with individuals with the C allele (OR = 1.07, 95% CI: 0.85-1.33, P = 0.57) in IL-6 polymorphism. However, statistical analysis showed that these differences are not significant. Our results suggested TGF-ß1 (codon 25 G/C) GG genotype may be considered as a risk factor for brucellosis (OR = 1.67, 95% CI: 1.12-2.50, P = 0.01). Herein, we failed to find any significant association between IL-10 (-1082 A/G, -819C/T), TGF-ß1 (codon 10C/T) gene polymorphism and susceptibility to brucellosis in all gene models. CONCLUSION: IL-6 (-174 G/C), IL-10 (-1082 A/G, -819C/T), and TGF-ß1 (codon 10C/T) polymorphisms is not a risk factor for brucellosis infection. TGF-ß1 codon 25 GG genotype may be considered as a risk factor for brucellosis.

[Effect of the Expression of iNOS Induced by Mycobacterium tuberculosis CRISPR-associated Csm4 (Rv2820c) on Intracellular Viability of Mycobacterium smegmatis].

OBJECTIVE: To determine how Csm4 protein expression affects intracellular survival of Mycobacterium smegmatis(MS). METHODS: Csm 4 gene was amplified by PCR to construct pMV261-Csm4 shuttle expression plasmid. The Csm4 protein expression in MS_Csm4 was detected by Western blot after electroporation of the recombinant plasmid into MS. The growth kinetics of MS_Csm4 in vitro and the influence of reactive N,O species on the growth of MS_Csm4were observed. The intracellular survival of MS_Csm4 and expressions of inducible nitric oxide synthase gene (iNOS) and nitric oxide production (NO) were detected after infection with THP-1 macrophages. RESULTS: Csm4 protein was successfully expressed in MS_Csm4,which did not affect the growth of the recombinant MS. Reactive N,O species decreased MS_Csm4 colony forming unit (CFU) in vitro. THP-1 increased the expression of iNOS and NO production and decreased intracellular survival of MS_Csm4. CONCLUSION: Recombinant MS_Csm4 is susceptible to reactive N,O species in vitro. THP-1 promotes NO release and thus discourages intracellular survival of MS.

The relationship between NTCP gene varieties and the progress of liver disease after HBV infection: an updated systematic review and meta-analysis.

BACKGROUND: The aim of this study was to analyze the relationship between sodium taurocholate cotransporting polypeptide (NTCP) gene varieties and hepatitis B virus (HBV) infection and the progress of HBV-related liver disease. METHODS: PubMed, EMBASE, Web of Science and Cochrane library were used to search eligible studies. STATA software was performed to combine results. Pooled odds ratios (OR) was used to assess the potential genetic relationships. RESULTS: A total of 18 eligible case-control studies with 24960 cases and 28342 controls were included in this meta-analysis. The A allele of rs2296651 polymorphism was found to be significantly linked to a protection of HBV infection in the whole combined analysis (P = 0.000). Meanwhile, this allele was significantly associated with a decreased risk of hepatocellular carcinoma (HCC) (A vs. G: OR = 0.668, 95% CI: 0.571-0.782, P = 0.000), and was significantly associated with HBV nature clearance (A vs. G: OR = 0.744, 95% CI: 0.585-0.946, P = 0.016; AA+GA vs. GG: OR = 0.775, 95% CI: 0.613-0.980, P = 0.033; GA vs. GG: OR = 0.748, 95% CI: 0.588-0.952, P = 0.018). However, rs4646287 genetic varieties had no statistical differences in all models with HBV infection or HBV-related disease progress, liver cirrhosis, acute-on-chronic liver failure and HCC, as well as rs7154439, rs4646285, rs4646296. CONCLUSIONS: Rs2296651 polymorphism (A allele) may protect from HBV infection and the progress of HBV-related disease (HBV-related HCC). Future research about other single nucleotide polymorphisms (SNPs) (rs4646287, rs7154439, rs4646285, rs4646296) of NTCP may be needed to clarify the relationship of NTCP gene varieties with HBV infection and HBV-related disease.

[Reconstruction of summer NDVI over the past 339 years based on the tree-ring width of Picea schrenkiana in Bayinbuluke, Central Tianshan, China]. / åŸºäºŽé›ªå²­äº‘æ‰æ ‘è½®å®½åº¦é‡å»ºè¿‡åŽ»339å¹´å¤©å±±ä¸­éƒ¨å·´éŸ³å¸ƒé²å ‹åœ°åŒºå¤å­£NDVI.

The normalized difference vegetation index (NDVI) is widely used in various fields of vegetation research. Due to the short observation time, however, it is difficult to meet the research needs at long time scale. Here, we established a tree-ring width chronology (STD) based on Picea schrenkiana in Bayinbuluke, and calculated the correlation coefficient of chronology and NDVI with meteorological data. The results showed that both tree-ring width index and NDVI were significantly correlated with meteorological data. Combined with the significant positive correlation between width chronology and NDVI in June-August (r=0.7, P<0.01, n=38), summer NDVI (from June to August) was reconstructed over the past 339 years using a regression model. During 1680-2018, the reconstruction series had four dense vegetation periods (1738-1765, 1786-1798, 1964-1973 and 2000-2018) and five sparse vegetation periods (1690-1714, 1825-1834, 1850-1880, 1895-1920 and 1945-1955). The reconstruction reflected the hydrological signals in the central Tianshan Mountains. The comparison with the surrounding reconstructions revealed that when the runoff of Kaidu River increased and the local environment was humid, the vegetation coverage was high; otherwise the vegetation coverage was low. The extreme value of the reconstruction series also captured a series of natural disasters recorded in historical documents. Results of HYSPLT (Hybrid Single-Particle Lagrangian Integrated Trajectory Model) backward trajectory model and wind field analysis showed that NDVI anomalies were affected by the precipitation from Westerlies.

The effect of CYP2C19 gene polymorphism on the eradication rate of Helicobacter pylori by proton pump inhibitors-containing regimens in Asian populations: a meta-analysis.

Premise: The effects of proton pump inhibitors (PPI) depend on metabolic enzyme CYP2C19 that has different activity due to gene polymorphism. The purpose of this meta-analysis is to determine the potential effects of CYP2C19 polymorphism on the efficiency of PPI-based treatment. Materials & methods: The PubMed, EMBASE, Cochrane Library, etc. were searched for relevant articles published in English or Chinese from inception to 31 May 2020. Finally, 26 randomized controlled trials and 15 cohort studies met the inclusion criteria and used for the meta-analysis via STATA version 15. Results: Poor metabolizer (PM) genotype Helicobacter pylori eradication rates were highest for Asian individuals receiving triple or quadruple first-line therapy based on PPIs (p < 0.05). CYP2C19 polymorphism could influence H. pylori eradication rate only in Mainland China and Japan (p < 0.05). Conclusion: PM genotype facilitates the elimination of H. pylori in Asian populations. Rabeprazole-, esomeprazole- and pantoprazole-based eradication program was less affected by the CYP2C19 polymorphism.

[Suitability assessment of construction land in the central and southern parts of Hebei Province, China based on potential-limitation model].

The suitability assessment of regional construction land is one of the important prerequisites for the spatial arrangement in regional planning, and also, the important foundation for the reasonable utilization of regional land resources. With the support of GIS, and by using the regional comprehensive strength and spatial accessibility analysis and the eco-environmental sensitivity analysis, this paper quantitatively analyzed the development potential and its ecological limitation of the central and southern parts of Hebei Province. Besides, based on the cost-benefit analysis, the potential-limitation model was accordingly developed, and the three land suitability scenarios under different developmental concepts were captured through the interaction matrix. The results indicated that both the comprehensive strength and the development potential of the study area showed a primacy distribution pattern, and presented an obvious pole-axis spatial pattern. The areas with higher eco-environmental sensitivity were mainly distributed in the west regions, while those with lower eco-environmental sensitivity were in the east regions. Regional economic development concept had important effects on the regional ecological security pattern and urban growth. The newly developed principles and methods for the land suitability assessment in this paper could not only scientifically realize the spatial grid of regional development potential and capture the future land development trend and spatial distribution, but also provide scientific basis and effective ways for urban and regional planning to realize region 'smart growth' and 'smart conservation'.

[Land use change and its effects on ecosystem services value in Ji' nan City of Shandong Province, East China].

Based on the GIS software platform, referring to the China 'Terrestrial ecosystem services per unit area value', and by using transition matrix, Costanza evaluation formula, and sensitivity analysis, this paper studied the change characteristics of land use and ecosystem services value in Ji' nan City in 1989-2009. During the study period, the built-up area in the City increased by 99.65 km2, while agriculture land and green space reduced by 103.21 km2, 90.4% of which was taken by the built-up land. The total ecosystem services value decreased from 256.22 x 10(6) yento 214.16 x 10(6) yen, with a decrement of 42.06 x 10(6) yen, mainly due to the decrease in the areas of agriculture land and green space. For the sustainable development of the population, resources, and environment in Ji'nan City, future urban planning should pay more attention on the natural resources protection, reasonable planning of land use structure, and maintenance of ecosystem stability and balance.