RESUMO
Bioassay-guided fractionation of the culture extract of Aspergillus wentii EN-48, an endophytic fungus isolated from an unidentified marine brown algal species of the genus Sargassum, led to the isolation of three new tetranorlabdane diterpenoids, asperolides A-C (1-3), and five related derivatives (4-8). The structures of these compounds were established on the basis of spectroscopic interpretation, and compound 1 was confirmed by X-ray crystallographic analysis. The absolute configuration of 1 was determined by application of the modified Mosher's method. An X-ray structure for wentilactone B (6) is also reported. Compounds 1-8 were evaluated for cytotoxic and antibacterial activities.
Assuntos
Aspergillus/química , Diterpenos/isolamento & purificação , Phaeophyceae/química , Ampicilina/farmacologia , Cristalografia por Raios X , Diterpenos/química , Diterpenos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Feminino , Células HeLa , Humanos , Biologia Marinha , Testes de Sensibilidade Microbiana , Conformação Molecular , Estrutura Molecular , Ressonância Magnética Nuclear BiomolecularRESUMO
A novel triazole carboxylic acid, penipanoid A (1), two new quinazolinone alkaloids, penipanoids B (2) and C (3), and a very recently reported quinazolinone derivative (4) were isolated from the marine sediment-derived fungus Penicillium paneum SD-44. Their structures were elucidated by spectroscopic analysis, and the structure for 1 was confirmed by X-ray crystallographic analysis. Compound 1 represents the first example of a triazole derivative from marine sediment-derived fungi, and compound 2 is a rare quinazolinone derivative having a dihydroimidazole ring system. The cytotoxicity of compounds 1 and 4 and the antimicrobial activity of 1-4 were evaluated.
Assuntos
Alcaloides/isolamento & purificação , Antineoplásicos/isolamento & purificação , Imidazóis/isolamento & purificação , Penicillium/química , Quinazolinonas/isolamento & purificação , Triazóis/isolamento & purificação , Alcaloides/química , Alcaloides/farmacologia , Antineoplásicos/química , Antineoplásicos/farmacologia , Ácidos Carboxílicos , Ensaios de Seleção de Medicamentos Antitumorais , Sedimentos Geológicos/química , Humanos , Imidazóis/química , Imidazóis/farmacologia , Testes de Sensibilidade Microbiana , Estrutura Molecular , Quinazolinonas/química , Quinazolinonas/farmacologia , Triazóis/química , Triazóis/farmacologiaRESUMO
Different kinds of mineral nutrients(NO3-N, NH4-N and PO4-P) were applied in the simulated oil-polluted seawater for enhancing oil biodegradation in the N/P ratio 10:1 and 20:1. Although indigenous microorganisms have the ability to degrade oil, adding nutrients accelerated biodegradation rates significantly. For the group amended with NO3-N and PO4-P in the ratio 10:1, the reaction rate coefficient was 4 times higher than the natural biodegradation. Chemical and microbiological analysis showed that the optimal N/P ratio in the system is 10:1, and microorganisms tend to utilize nitrate rather than ammonium as N source.