Effect of water scarcity during thermal-humidity exposure on the mineral footprint of sheep.

OBJECTIVE: Combination of two stressors on alteration of mineral footprints in animals needs due attention to meet maximum production and welfare, particularly in grazing sheep. This study tested whether ewes (Ovis aries) exposed to water deprivation and thermal-humidity stressors had altered mineral footprints in their wool, serum, urine, and feces. METHODS: Nine ewes (age = 3 years; mean body weight = 41±3.5 kg) were divided among a control group with free access to water, and treatment groups with water deprivation lasting either 2 h (2hWD) or 3 h (3hWD) after feeding. Using a 3×3 Latin square design, animals were assigned to treatment groups for three sampling periods of 21 days each (n = 9). Blood was collected by jugular venipuncture. Wool was collected at the end of periods 2 and 3. Metabolic crates designed with metal grated floors were used for urine and feces collection. We measured sodium (Na), magnesium (Mg), phosphorus (P), chloride (Cl), calcium (Ca), manganese (Mn), copper (Cu), iron (Fe), and zinc (Zn). RESULTS: The wool mineral levels did not differ between the treatment groups, although K was marginally lower (p = 0.10) in the 2hWD group. The serum and urine mineral levels did not differ between the treatments (p>0.05). Fecal K was significantly lower in the 2hWD group than in the other groups (p≤0.05). CONCLUSION: In conclusion, water deprivation and thermal-humidity exposure altered the excretion of K, but not of other minerals, in the wool, urine, feces, or serum of ewes. Thus, no additional mineral supplementation is needed for water deprived ewes during thermalhumidity exposure.

In vivo evaluation of garlic (Allium sativum) supplementation to rice straw-based diet on mitigation of CH4 and CO2 emissions and blood profiles using crossbreed rams.

BACKGROUND: Two experiments were implemented using three cannulated crossbreed rams (Corriedale × Suffolk) fed rice straw-based diet. In vivo evaluation of garlic powder (GP) supplementation to rice straw-based diet on CH4 and CO2 emissions and blood profiles of the rams (body weight = 52 ± 3 kg) was performed. The design was a triplicate 3 × 3 Latin square. Groups were the control (no GP) and two GP groups, which were given supplementation for 0.1 (GP0.1) and 0.5% (GP0.5) of concentrate. The differences between two experiments were data missing of GP0.1 and uncontrolled environment in Experiment 1. Blood profiles were added in Experiment 2. RESULTS: In Experiment 1, supplementation of GP to the rams reduced CH4 production significantly (P < 0.05) by 38%. Dry matter intake was not different among the groups in both experiments. In Experiment 2, CH4 was lower in the GP0.5 group than the control group by 7%, whereas CO2 showed no difference among the groups. Serum glutamic oxaloacetic transaminase was significantly (P > 0.05) lower in the GP0.5 group than the control group, whereas glutamic pyruvic transaminase, total protein, urea N, total cholesterol, low-density lipoprotein, calcium, phosphorus, magnesium and non-esterified fatty acids were not significantly (P > 0.05) different. CONCLUSIONS: GP supplementation, particularly GP0.5, decreases CH4 emissions in rams fed rice straw-based diets. © 2018 Society of Chemical Industry.

Effects of water addition to total mixed ration on water intake, nutrient digestibility, wool cortisol and blood indices in Corriedale ewes.

OBJECTIVE: The objective of this study was to determine the effect of adding water to total mixed ration (TMR) on fresh water intake, nutrient digestibility, wool cortisol, and blood indices in Corriedale ewes under hot and humid conditions. METHODS: Nine non-pregnant Corriedale ewes (ave. body weight = 41±3.5 kg) were individually fed diets based on maintenance requirements in metabolic crates. Ewes were assigned to three treatment groups according to a triplicate 3×3 Latin Square design for 3 periods of 21 days duration each (9 ewes per treatment, 27 replications). Treatments were TMR (crude protein [CP] = 16.1, total digestible nutrients = 69.1%) moisture levels for 40%, 50%, and 60%. RESULTS: No differences were found in body weight gain among all treatment groups (p>0.05). Nitrogen balance including digestible N, retained N, and urinary and fecal N showed no change among the treatment groups (p>0.05). Fresh water intake was the lower in 50% TMR moisture group than in the other groups (p<0.05). Other than ether extract which was higher in 60% TMR moisture group (p<0.05) the differences among nutrient digestibilities including CP, organic matter, dry matter, neutral detergent fiber, acid detergent fiber, and non-fiber carbohydrate were not significant (p>0.05). No significant difference was observed for serum protein, blood urea nitrogen, glucose, and triglyceride among the treatment groups (p>0.05). Wool and blood cortisol were not different among the treatment groups (p>0.05). Blood hematology including red blood cell, white blood cells, hemoglobin, hematocrit, basophils, and eosinophils were not different among the treatment groups (p>0.05). CONCLUSION: It is concluded that TMR moisture at 40%, 50%, and 60% had no effects on N balance parameters, and nutrient digestibilities except for the ether extract under hot and humid conditions. Additionally there were no effects on stress conditions include wool cortisol, as well as blood cortisol levels of ewes.

Effects of Chromium Methionine Supplementation on Blood Metabolites and Fatty Acid Profile of Beef during Late Fattening Period in Holstein Steers.

The objective of this study was to determine the effects of chromium methionine (Cr-Met) chelate supplementation on blood metabolites and fatty acid profile of beef from Holstein steers during late fattening period. Fifteen Holstein steers were allotted randomly into two groups including the control (non Cr-Met feeding, NCM, ave. body weight [BW] = 483±25.7 kg) and the treatment (Cr-Met feeding for 4 months, 4CM, ave. BW = 486±27.5 kg) group. The feeding amount of Cr-Met to animals was limited to 400 ppb/cow/d and was supplemented to total mixed ration. No difference in blood albumin, alkaline phosphatase, urea-nitrogen, calcium, creatine, glucose, total protein, triglyceride, and cholesterol were observed between the treatment groups (p>0.05). The level of high density lipoprotein was higher in the 4CM group than the NCM group, whereas low density lipoprotein was lower in the 4CM group (p<0.05). The fatty acid composition (caprate, laurate, myristate, pentadecanoate, palmitate, palmitoleate, margarate, cis-11 heptadodecanoate, stearate, oleate, trans-vaccenate, linoleate, cis-11 eicosenoate, docosa hexaenoic acid, and docosa pentaenoic acid) of the beef showed no difference between the two groups (p>0.05). The arachidonic acid level tended to be higher in the 4CM than the NCM group (p = 0.07). Cr-Met had no influence (p>0.05) on the ratio of saturated, unsaturated, unsaturated/saturated, monounsaturated/saturated and polyunsaturated/saturated fatty acids whereas the ratio of polyunsaturated fatty acids (PUFA) in the 4CM group was comparatively higher than the NCM group (p<0.05). This study concluded that feeding Cr-Met supplementation in 400 ppb/d to Holstein steers for 4 months during late fattening period can improve some blood metabolites and beef quality by increasing PUFA and gamma-linoleate compositions of beef.

Assessment of growing condition variables on alfalfa productivity.

This study was conducted to assess the impact of growing condition variables on alfalfa (Medicago sativa L.) productivity. A total of 197 alfalfa yield results were acquired from the alfalfa field trials conducted by the South Korean National Agricultural Cooperative Federation or Rural Development Administration between 1983 and 2008. The corresponding climate and soil data were collected from the database of the Korean Meteorological Administration. Twenty-three growing condition variables were developed as explaining variables for alfalfa forage biomass production. Among them, twelve variables were chosen based on the significance of the partial-correlation coefficients or potential agricultural values. The selected partial correlation coefficients between the variables and alfalfa forage biomass ranged from -0.021 to 0.696. The influence of the selected twelve variables on yearly alfalfa production was summarized into three dominant factors through factor analysis. Along with the accumulated temperature variables, the loading scores of the daily mean temperature higher than 25°C were over 0.88 in factor 1. The sunshine duration at temperature between 0°C-25°C was 0.939 in factor 2. Precipitation days were 0.82, which was the greatest in factor 3. Stepwise regression applied with the three dominant factors resulted in the coefficients of factors 1, 2, and 3 for 0.633, 0.485, and 0.115, respectively, and the R-square of the model was 0.602. The environmental conditions limiting alfalfa growth, such as daily temperature higher than 25°C or daily mean temperature affected annual alfalfa production most substantially among the growing condition variables. Therefore, future cultivar selection should consider the capability of alfalfa to be tolerant to extreme summer weather along with biomass production potential.

Effects of Dietary Garlic Extract on Growth, Feed Utilization and Whole Body Composition of Juvenile Sterlet Sturgeon (Acipenser ruthenus).

This study was carried out to investigate the supplemental effects of dietary garlic extract (GE) on growth performance of juvenile sterlet sturgeon (Acipenser ruthenus). The first experiment was designed to determine the optimum levels of garlic extract as growth promoter during 10 weeks. Three groups (two replicates/group) of 240 fish with mean body weight of 85 g were fed with diets containing 0 (control), 0.5 and 1.0% of GE. The highest weight gain (%) and feed efficiency (%) were found in fish groups fed with diet containing 0.5% GE. Subsequently, the supplemental effects of dietary GE was studied on growth of juvenile sterlet sturgeon (Acipenser ruthenus) with an average body weight of 59.6 g. Fish cultured in freshwater were randomly allotted to each of 10 tanks (two groups of five replicates, 20 fish/tank) and fed diets with 0.5% GE or without GE (control), respectively, at the level of 2.0% of fish body weight per day for 5 weeks. Weight gain (51.1%), feed efficiency (79.1%), specific growth rate (1.18%) and protein efficiency ratio (1.50) of fish fed 0.5% GE were significantly (p<0.05) higher than those fish fed the control diet. Significantly higher protein (PRE 20.4%) and lipid retention efficiencies (LRE, 74.5%) were also found in 0.5% GE group (p<0.05). The present results suggested that dietary GE could improve growth and feed utilization of juvenile sterlet sturgeons.

Daytime Grazing in Mountainous Areas Increases Unsaturated Fatty Acids and Decreases Cortisol in the Milk of Holstein Dairy Cows.

The effects of grazing lactating cows in mountainous areas for 12 and 24 h compared with the confined indoor system were evaluated by examining the overall milk fatty acid and cortisol. Twenty-one dairy cows were allocated to three treatment groups: (1) control (confined management system in a free-stall barn; TMR based), (2) grazing for 12 h (12 hG; TMR plus grazing pasture), and (3) grazing for 24 h (24 hG; pasture-based feeding system). Dry matter intake was higher in the control and 12 hG groups than in the 24 hG group. The yields of total milk and the 3.5% fat-corrected milk were the lowest in the 24 hG group. Milk fat was the highest in the 24 hG group and higher in 12 hG compared with the control group. Milk protein and lactose levels were the highest in the 12 hG group. The highest somatic cell count was observed in the 24 hG group. The saturated fatty acid levels were higher in the control group compared with the 12 hG and 24 hG groups. There was no difference in overall mono-unsaturated fatty acids between 12 hG and 24 hG groups. Poly-unsaturated fatty acids were higher in the 12 hG group compared with the control and 24 hG groups. There was no difference in omega-6 (ω-6) fatty acids among the groups, and omega-3 fatty acids were higher in the 12 hG group than in the control group. Milk cortisol was the highest in the 24 hG group and higher in the control group compared with the 12 hG group. Taken together, grazing for 12 h is advisable for farms that have access to mountainous areas to improve the milk fatty acid profile and decrease the stress levels in high-yielding Holstein lactating cows.

In vivo nutritive value of rice feed for sheep and its application for cattle feed.

The objective of this study was to evaluate the nutritive value of polished rice (PR) vs unpolished rice (UPR) as a potential feedstuff for sheep in order to use as a replacer to corn in sheep diet, and as well as to present the application in the formulation of cattle diet. Six corriedale ewe were randomly assigned to each treatment. UPR and PR were provided as a dietary treatment together with timothy grass as a basal diet in a crossover design for two period with 15-d duration for each period. The ratio of experimental and basal feeds were 33.3% and 66.7%, respectively. The differences in the total digestible nutrient (TDN) contents between sheep and cattle was determined according to the references. The number of data collected sheep and cattle was 9 and 17, respectively. The PR showed higher nutrients digestibility than UPR. Similarly, higher TDN content was observed PR than UPR (p < 0.05). As a result, the replacement of corn in the formulate feed with UPR and PR feed rice could be possible with the ratio of 91.2% and 100.0%, respectively. The result of comparation the TDN contents of UPR and PR in sheep and cattle, the PR has no difference in the nutritive value which suggests the applicability of the results of sheep to cattle. On the other hand, UPR has known to have different nutritive value between sheep and cattle, so caution should be taken when preparing formula feeds for cattle.

Behavioral and physiological changes during heat stress in Corriedale ewes exposed to water deprivation.

This study was conducted to investigate the behavioral and physiological changes of heat stressed Corriedale ewes exposed to water deprivation. Nine Corriedale ewes (average BW = 45 ± 3.7 kg) were individually fed diets based on maintenance requirements in metabolic crates. Ewes were assigned into three groups (9 sheep per treatment) according to a 3 × 3 Latin square design for 3 periods with 21-d duration for each period. The control (CON) group was given free access to water, 2 h water deprivation (2hWD), and 3 h water deprivation (3hWD) following feeding. No differences were found in fecal excretion frequency, standing frequency (number/d), and sitting frequency among the groups (p > 0.05). Measurements of standing duration (min/d) and urine excretion frequency (number/d) showed a significant decrease whereas sitting duration (min/d) showed a significant increase in the 2hWD and 3hWD groups when compared with the CON group (p < 0.05). Fecal score and heart rate (number/min) were not different among the groups (p > 0.05). However, respiratory rate (number/min) and panting score were found to be significantly higher in the 2hWD and 3hWD groups than in the CON group (p < 0.05). It is concluded that water deprivation following feeding intensifies physiological heat stress related indicators such as respiratory rate and panting score and changes behavioral parameters such as water intake and urine excretion frequency in heat stressed ewes. Daily adaptation to the extreme environmental conditions may occur actively in ewes.

Coat and hair color: hair cortisol and serotonin levels in lactating Holstein cows under heat stress conditions.

The deleterious effects of heat stress on animal health are being increasingly recognized. This study aimed to determine hair cortisol (HC) and serotonin levels in lactating Holstein cows under heat stress conditions with different coat and hair-cut color. Forty-five multiparous lactating Holstein cows (days in milk = 130 ± 47, body weight = 753 ± 85 kg) were divided to two main groups of over 80% black coat color (BC) and over 85% white coat color (WC) visually observed based on registry certificates and subdividing to black hair sample (BH) and white hair samples (WH) in 2 × 2 factorial arrangements. Hair samples were taken from the forehead of the individuals. Higher HC levels were observed in BC than WC cows (P < 0.05). No differences were found in HC levels between BH and WH groups (P > 0.05). Serotonin levels showed no difference between BC and WC (P > 0.05). Interaction between coat color and hair color was not significant (P > 0.05). The cortisol levels in hair are not affected by pigmentation. However, pigmentation within the coat alters cortisol levels. In conclusion, white coat color retains less cortisol than the black coat. Therefore, white coats are preferable for dairy cows under heat stress conditions.

Effects of forage level and chromium-methionine chelate supplementation on performance, carcass characteristics and blood metabolites in Korean native (Hanwoo) steers.

A feeding trial was carried out to determine the effects of chromium methionine (Cr-Met) chelate and forage level over two years, 1(st) fattening and 2(nd) fattening period on growth parameters, carcass characteristics and blood metabolites of 46 Korean native (Hanwoo, Bos Taurus, BW = 183 ± 44 kg) steers. Treatments were: 1) Steers in the low forage (LF) group were fed diets that consisted of 60% concentrate and 40% forage; 2) Steers in the high forage (HF) group were fed diets that consisted of 40% concentrate and 60% forage. Following the 1(st) fattening period, steers (BW = 480 ± 37.6 kg) were randomly assigned to four treatment groups: LF (40 F plus no Cr-Met supplementation in the 2(nd) fattening period), LFCM (40LF plus added 400 ppb of Cr-Met during the 2(nd) fattening period), HF (60 F plus no added Cr-Met during the 2(nd) fattening period) and HFCM (60 F plus added 400 ppb of Cr-Met in the 2(nd) fattening period). Dry matter intake of the treatment diets did not differ during the raising and 1(th) fattening period (P > 0.05). The ADG in the raising period showed no difference between the 40 F and 60 F groups (P > 0.05). Carcass characteristics including rib-eye area and meat yield index were higher in HF than the other treatment groups (P < 0.05). The HF group tended to show a higher (P = 0.08) marbling score than the LF group whereas the HFCM group showed a higher marbling score than the LFCM group (P < 0.05). HDL was higher and LDL lower in groups fed with Cr-Met than in other groups whereas glucose showed the lowest value in HF group (P < 0.05). Triglyceride (TG), Cholesterol, PUN and total protein (TP) were the same among all treatment groups (P > 0.05). The Insulin concentration in the blood was significantly higher for the HFCM group than for the LF, LFCM and HF groups (P < 0.05). It is concluded that supplementation of chromium-methionine chelate could improve meat quality in beef steers.