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Anthropogenic activities are leaving lots of chemical footprints on the soil. It alters the physiochemical characteristics of the soil thereby modifying the natural soil microbiome. The prevalence of antimicrobial-resistance microbes in polluted soil has gained attention due to its obvious public health risks. This study focused on assessing the prevalence and distribution of antibiotic-resistance genes in polluted soil ecosystems impacted by industrial enterprises in southern Russia. Metagenomic analysis was conducted on soil samples collected from polluted sites using various approaches, and the prevalence of antibiotic-resistance genes was investigated. The results revealed that efflux-encoding pump sequences were the most widely represented group of genes, while genes whose products replaced antibiotic targets were less represented. The level of soil contamination increased, and there was an increase in the total number of antibiotic-resistance genes in proteobacteria, but a decrease in actinobacteria. The study proposed an optimal mechanism for processing metagenomic data in polluted soil ecosystems, which involves mapping raw reads by the KMA method, followed by a detailed study of specific genes. The study's conclusions provide valuable insights into the prevalence and distribution of antibiotic-resistance genes in polluted soils and have been illustrated in heat maps.
Assuntos
Metais Pesados , Hidrocarbonetos Policíclicos Aromáticos , Microbiologia do Solo , Poluentes do Solo , Poluentes do Solo/análise , Poluentes do Solo/toxicidade , Metais Pesados/análise , Metais Pesados/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/análise , Resistência Microbiana a Medicamentos/genética , Federação Russa , Metagenômica , Genes Bacterianos , Farmacorresistência Bacteriana/genética , Monitoramento AmbientalRESUMO
Climate change is a global problem that is accompanied by the significant changes in humidification conditions in many regions all over the world. The study examined spatiotemporal changes in humidification zones in southern Russia in the period 1961-2020. Humidification zones were determined in accordance with the classification of the Selyaninov hydrothermal coefficient. During the research period, a significant increase in average annual temperatures was noted for the region (+ 0.31 °C/decade) and generally positive, but insignificant, dynamics of annual precipitation (+ 4.80 mm/decade). These changes were accompanied by a significant shift in the humidification zones. Despite some periods of decline, the territories of semi-desert and desert zones, as well as dry steppes, have significantly expanded from the east of the region to the west in the last decade. The expansion of these zones was primarily due to a reduction in the area of the more humid steppe zone, with a lesser contribution from the forest-steppe zone. Overall, during the study period, the area of semi-desert and desert zone expanded by an average of + 5.505 thou km2/decade. The zone of the Taiga and deciduous forests has not undergone significant changes. The results obtained indicate arid warming and a general deterioration in humidification conditions in most of southern Russia in the period 1961-2020. According to calculations, further warming, other things being equal, can lead to an even greater expansion of the semi-desert and desert bioclimatic zone, which can provoke a number of socio-economic and environmental problems, especially in the eastern part of the region.
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DFOS (distributed fiber-optic sensing) technology has shown the potential to increase the accuracy of measurement after years of development and experimenting in geoengineering monitoring. To better understand the development of DFOS technology and its contribution to geoengineering, an objective and data-driven review of the development process of DFOS technology in construction was completed. The review was accomplished by using text mining methods on the Web of Science, covering a wide range of relevant data, including 3970 articles from 1989 to 2023. The results indicate that DFOS technology research demonstrates the typical characteristics of multi-author, multi-country, and multi-institution collaborations, spanning various research fields. Over the past 35 years, the number of published articles has exhibited exponential growth, with China making significant contributions and leading in terms of its total publication growth rate, which has been higher than that of the United States since 2016. In the analysis of author keywords, emerging technologies, such as machine learning and distributed acoustic sensing, have garnered attention. The findings contribute to a comprehensive understanding of the development, impact, and future trends of DFOS technology in geotechnical engineering, offering valuable insights for researchers, scholars, and students in the field and inspiring new approaches for research methods in this domain.
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The combustion of coal in power plants releases significant amounts of polycyclic aromatic hydrocarbons (PAHs), which are highly toxic and carcinogenic. This study assesses the ecological and human health impacts of PAHs contamination from a coal-fired power plant over 8 years. The monitoring site selection considered the distance from the power plant and the prevailing wind direction in the investigated area. The results reveal that, during the monitoring period, PAH levels increased on average by 43%, 61%, and 37% in the zone of the prevailing wind direction, in the area proximate to the power plant, and the zone distant from it, respectively. The site, which has a radius of 4.5 km in the prevailing wind direction, exhibited the highest ecological and human health impacts. Additionally, a strong correlation was observed between environmental and human health impacts, depending on the distance from the power plant, particularly in areas with the prevailing wind direction. These insights contribute to a comprehensive understanding of the intricate dynamics linking power plant emissions, PAHs contamination, and their far-reaching consequences on the environment and human health.
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Carvão Mineral , Monitoramento Ambiental , Hidrocarbonetos Policíclicos Aromáticos , Centrais Elétricas , Poluentes do Solo , Hidrocarbonetos Policíclicos Aromáticos/análise , Humanos , Monitoramento Ambiental/métodos , Poluentes do Solo/análise , Vento , Avaliação do Impacto na SaúdeRESUMO
Benzo[a]pyrene (BaP) is noted as one of the main cancer-causing pollutants in human beings and may damage the development of crop plants. The present work was designed to explore more insights into the toxic effects of BaP on Solanum lycopersicum L. at various doses (20, 40, and 60 MPC) spiked in Haplic Chernozem. A dose-dependent response in phytotoxicity were noted, especially in the biomass of the roots and shoots, at doses of 40 and 60 MPC BaP and the accumulation of BaP in S. lycopersicum tissues. Physiological and biochemical response indices were severely damaged based on applied doses of BaP. During the histochemical analysis of the localization of superoxide in the leaves of S. lycopersicum, formazan spots were detected in the area near the leaf's veins. The results of a significant increase in malondialdehyde (MDA) from 2.7 to 5.1 times, proline 1.12- to 2.62-folds, however, a decrease in catalase (CAT) activity was recorded by 1.8 to 1.1 times. The activity of superoxide dismutase (SOD) increased from 1.4 to 2, peroxidase (PRX) from 2.3 to 5.25, ascorbate peroxidase (APOX) by 5.8 to 11.5, glutathione peroxidase (GP) from 3.8 to 7 times, respectively. The structure of the tissues of the roots and leaves of S. lycopersicum in the variants with BaP changed depending on the dose: it increased the intercellular space, cortical layer, and the epidermis, and the structure of the leaf tissues became looser.
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Benzo(a)pireno , Solanum lycopersicum , Antioxidantes , Benzo(a)pireno/química , Benzo(a)pireno/toxicidade , Catalase , Glutationa Peroxidase , Solo/química , Solanum lycopersicum/química , Solanum lycopersicum/metabolismo , Superóxido DismutaseRESUMO
Worldwide industrialization and other human activities have led to a frightening stage of release of hazardous, highly persistent, toxic, insoluble, strongly adsorbed to the soil and high molecular weight ubiquitous polycyclic aromatic hydrocarbons (PAHs) in soils and sediments. The various conventional remediation methods are being used to remediate PAHs with certain drawbacks. Time taking process, high expenditure, excessive quantities of sludge generation, and various chemical requirements do not only make these methods outdated but produce yet much resistant and toxic intermediate metabolites. These disadvantages may be overcome by using a microbial electrochemical system (MES), a booming technology in the field of bioremediation. MES is a green remediation approach that is regulated by electrochemically active microorganisms at the electrode in the system. The key advantage of the system over the conventional methods is it does not involve any additional chemicals, takes less time, and generates minimal sludge or waste during the remediation of PAHs in soils. However, a comprehensive review of the MES towards bioremediation of PAHs adsorbed in soil and sediment is still lacking. Therefore, the present review intended to summarize the recent information on PAHs bioremediation, application, risks, benefits, and challenges based on sediment microbial fuel cell and microbial fuel cell to remediate mount-up industrial sludge, soil, and sediment rich in PAHs. Additionally, bio-electrochemically active microbes, mechanisms, and future perspectives of MES have been discussed.
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Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Humanos , Hidrocarbonetos Policíclicos Aromáticos/análise , Solo/química , Esgotos , Poluentes do Solo/análise , Biodegradação AmbientalRESUMO
Polycyclic aromatic hydrocarbons (PAHs), as priority organic pollutants, are capable of accumulation in plants. Phenanthrene (Phe) is one of the most abundant low-molecular-weight PAH in the environment which is commonly used as a model PAH in many phytoremediation studies and as a representative compound for all PAHs group. This paper highlights the uptake, translocation, and accumulation of Phe by growing proso millet (Panicum miliaceum L.) in a pot experiment, subjected to 500, 1000, 1500, and 2000 ppm of Phe treatment after 15 and 30 days. Phe naturally existed in P. miliaceum and its concentration showed a time-dependent reduction in treated plant tissues as well as in perlites. Phe concentration in shoots was higher than in roots. During the aging process, the uptake of Phe was diminished whereas translocation factor (TF) demonstrated an overall increasing trend among treatments. The shoot concentration factor (SCF) values were higher than those of root concentration factor (RCF) on both days 15 and 30 and the highest values for both parameters were achieved in 500 ppm of Phe. Both RCFs and SCFs generally tended to decrease with the increase of perlite Phe concentrations. These results suggested that Phe tended to transfer to the shoots and be metabolized there. The Phe concentration revealed a significant decline in all levels of treatment on both 15 (84 to 96%) and 30 (76 to 94%) days. Therefore, the presence of P. miliaceum was effective in promoting the phytoremediation of Phe polluted perlites.
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Panicum , Fenantrenos , Hidrocarbonetos Policíclicos Aromáticos , Panicum/metabolismo , Fenantrenos/metabolismo , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Transporte BiológicoRESUMO
PAHs are one of the most toxic organic compounds classes which is obligatory controlled all over the world. There is a luck of studies devoted to the PAHs levels and sources identification in the south of Russia. The features of the PAHs accumulation and spatial distribution in hydromorphic soils (Fluvisol) were studied on the example of the soils of the Don River delta floodplain landscapes. It has been shown that changes in the PAHs content in soils depended on the type and intensity of the emission source. A factor analysis and multivariate linear regression analysis were carried out to determine the features of the spatial distribution for individual PAH compounds, considering the properties of soils and typical differences in the emission source. The most polluted areas in the studied area located along the transit line of the long-distance tankers, where the content of the most toxic high molecular PAHs compounds reached 8862 ng g-1. As a result of regression analysis, a relationship was established between the PAHs accumulation rate with the content of silt (particles less than 0.001 mm in size) and Ca2+ and Mg2+ exchangeable cations in the soil (at p-level < 0.0001). Differences in individual PAH content for medium and heavy loamy Fluvisol and depend on the influence of different types of pollution sources.
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Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Monitoramento Ambiental , Solo , Rios , Poluentes do Solo/análise , Hidrocarbonetos Policíclicos Aromáticos/análise , ChinaRESUMO
Soil decontamination and restoration continue to be a key environmental concern around the globe. The degradation of soil resources due to the presence of potentially toxic elements (PTEs) has a substantial influence on agricultural production, food security, and human well-being, and as a result, urgent action is required. PTEs pollution is not a threat to the agroecosystems but also a serious concern to human health; thereby, it needs to be addressed timely and effectively. Hence, the development of improved and cost-effective procedures to remove PTEs from polluted soils is imperative. With this context in mind, current review is designed to distinctly envisage the PTEs removal potential by the single and binary applications of biochar (BC) and nanomaterials (NMs).2 Recently, BC, a product of high-temperature biomass pyrolysis with high specific surface area, porosity, and distinctive physical and chemical properties has become one of the most used and economic adsorbent materials. Also, biochar's application has generated interest in a variety of fields and environments as a modern approach against the era of urbanization, industrialization, and climate change. Likewise, several NMs including metals and their oxides, carbon materials, zeolites, and bimetallic-based NMs have been documented as having the potential to remediate PTEs-polluted environments. However, both techniques have their own set of advantages and disadvantages, therefore combining them can be a more effective strategy to address the growing concern over the rapid accumulation and release of PTEs into the environment.
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Nanoestruturas , Poluentes do Solo , Humanos , Solo/química , Poluentes do Solo/análise , Carvão Vegetal/químicaRESUMO
The rapid development of industrialization is causing several fundamental problems in plants due to the interaction between plants and soil contaminated with metallic nanoparticles (NPs). Numerous investigations have been conducted to address the severe toxic effects caused by nanoparticles in the past few decades. Based on the composition, size, concentration, physical and chemical characteristics of metallic NPs, and plant types, it enhances or lessens the plant growth at various developmental stages. Metallic NPs are uptaken by plant roots and translocated toward shoots via vascular system based on composition, size, shape as well as plant anatomy and cause austere phytotoxicity. Herein, we tried to summarize the toxicity induced by the uptake and accumulation of NPs in plants and also we explored the detoxification mechanism of metallic NPs adopted by plants via using different phytohormones, signaling molecules, and phytochelatins. This study was intended to be an unambiguous assessment including current knowledge on NPs uptake, accumulation, and translocation in higher plants. Furthermore, it will also provide sufficient knowledge to the scientific community to understand the metallic NPs-induced inhibitory effects and mechanisms involved within plants.
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Nanopartículas Metálicas , Nanopartículas Metálicas/toxicidade , Plantas , Raízes de Plantas , Desenvolvimento VegetalRESUMO
The effect of heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) pollution on the microbiological status of soils on the coast of the Taganrog Bay and adjacent areas was studied. The content of total and exchangeable forms of HMs, the content of 16 priority PAHs and the abundance of several groups of culturable microorganisms was determined, namely copiotrophic, prototrophic, aerobic spore-forming bacteria, actinomycetes, molds and yeasts. The content of total and exchangeable forms of HMs in urban coastal soils in industrial zone significantly exceeded that in non-urban soils. The maximum concentrations of total forms of Mn, Cr, Ni, Cu, Zn, Pb and Cd are 1821, 871, 143, 89, 1390, 317 and 10 mg/kg, respectively. The median value of the total content of 16 PAHs in urban soils is 3 times higher than in the soils of natural areas and reached 4309 ng/g. The lowest numbers of copiotrophic bacteria, prototrophic bacteria and aerobic spore-forming bacteria were found in the soils of industrial zone: 6.8, 13.8 and 0.63 million CFU g-1 dry soil, respectively. The largest numbers of copiotrophic bacteria, prototrophic bacteria and aerobic spore-forming bacteria were recorded in the soils of natural areas-72.5, 136 and 5.73 million CFU g-1 dry soil, respectively. It was found that the abundance of copiotrophs, prototrophs, and aerobic spore-forming bacteria is more affected by the urbanization of coastal soils including the pollution of HMs and PAHs. Other groups of microorganisms (actinomycetes, molds and yeasts) turned out to be more resistant to anthropogenic factors.
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Actinobacteria , Metais Pesados , Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Solo , Hidrocarbonetos Policíclicos Aromáticos/análise , Baías , Poluentes do Solo/análise , Monitoramento Ambiental , Metais Pesados/análise , China , Medição de RiscoRESUMO
The pollution of floodplain, deltaic and adjacent coastal soils in large fluvial systems, considered an urgent environmental problem, as well as potentially toxic elements in such environments, can negatively affect aquatic ecosystems, as well as pose significant risks to human health. This paper is devoted to the geochemistry of potentially toxic elements in soils of the Lower Don basin, which is one of the largest and most anthropogenically transformed water bodies in Southern Russia, as well as the adjacent areas of the Taganrog Bay coast. The median element concentrations in the soils of the study area were consistent with the world soil average and the contents of elements in background soils. Comparative assessment of the spatial distributions as well as the results of Pearson's correlations, cluster analysis and principal component analysis showed that Cr, Ni, Cu and Zn are predominantly of natural origin; Mn and As are of mixed sources; and Cd and Pb are predominantly of anthropogenic origin. The geochemical anomalies of elements were associated with the impact of local anthropogenic sources. Geochemical background values for Cr, Mn, Ni, Cu, Zn, As, Cd and Pb in the soils of the Lower Don and the Taganrog Bay coast determined using the 'median + 2 median absolute deviations' approach are presented. The highest values of the integrated pollution indices were observed in floodplain soils of small rivers.
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Metais Pesados , Poluentes do Solo , Humanos , Solo/química , Monitoramento Ambiental/métodos , Metais Pesados/análise , Ecossistema , Baías , Cádmio/análise , Chumbo/análise , Poluentes do Solo/análise , Medição de Risco , ChinaRESUMO
In the current study, two plants, viz., Pisum sativum L. and Hordeum vulgare L., were exposed to nano- and macro-dispersed ZnO at 1, 10, and 30 times of maximal permissible concentration (MPC). The main objective of the study is to depict and compare the genotoxicity in terms of chromosomal anomalies, cytotoxicity (i.e., mitotic index), and phytotoxicity (viz., germination, morphometry, maximal quantum yield, and chlorophyll fluorescence imaging) of macro- and nano-forms of ZnO along with their accumulation and translocation. In the case of genotoxic and cytotoxic responses, the maximal effect was observed at 30 MPC, regardless of the macro- or nano-forms of ZnO. The phytotoxic observations revealed that the treatment with macro- and nano-forms of ZnO significantly affected the germination rate, germination energy, and length of roots and shoots of H. vulgare in a dose-dependent manner. The factor toxicity index of treated soil demonstrated that toxicity soared as concentrations increased and that at 30 MPC, toxicity was average and high in macro- and nano-dispersed ZnO, respectively. Furthermore, the photosynthetic parameters were observed to be negatively affected in both treatments, but the maximal effect was observed in the case of nano-dispersed form. It was noted that the mobility of nano-dispersed ZnO in the soil was higher than macro-dispersed. The increased mobility of nano-dispersed ZnO might have boosted their accumulation and translocation that subsequently led to the oxidative stress due to the accelerated production of reactive oxygen species, thus strengthen toxicity implications in plants.
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Óxido de Zinco , Óxido de Zinco/toxicidade , Raízes de Plantas , Fotossíntese , Solo , Dano ao DNARESUMO
The poultry industry is generating a significant amount of waste from chicken droppings that are abundant in microbes as well as macro- and micronutrients suitable for manure. It has the potential to improve the microbial activity and nutrient dynamics in the soil, ultimately improving soil fertility. The present study aimed to investigate the effect of chicken droppings manure (CDM) on the diversity of the soil microbiome in the free walking chicken's area located in Stefanidar, Rostov Region, Russia. The data obtained were compared with 16 s rRNA from control samples located not far from the chicken's free-walking area, but not in direct contact with the droppings. Effect of CDM on the physicochemical characteristics of the soil and changes in its microbial diversity were assessed by employing the metagenomic approaches and 16 s rRNA-based taxonomic assessment. The alpha and beta diversity indices revealed that the application of the CDM significantly improved the soil microbial diversity. The 16S taxonomical analysis confirmed Proteobacteria, Actinobacteria, Bacteroidetes, Firmicutes, and Planctomycetes as abundant bacterial phylum. It also revealed the increase in the total number of the individual operational taxonomic unit (OTU) species, a qualitative indicator of the rich microbial community. The alpha diversity confirmed that the significant species richness of the soil is associated with the CDM treatment. The increased OTUs represent the qualitative indicator of a community that has been studied up to the depth of 5-20 cm of the CDM treatment range. These findings suggested that CDM-mediated microbial richness are believed to confer the cycling of carbon, nitrogen, and sulfur, along with key soil enzymes such as dehydrogenases and catalase carbohydrate-active enzymes. Hence, the application of CDM could improve soil fertility by nutrient cycling caused by changes in soil microbial dynamics, and it could also be a cost-effective sustainable means of improving soil health.
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Microbiota , Solo , Animais , Galinhas , Esterco , Aves Domésticas , Microbiologia do Solo , Bactérias/genéticaRESUMO
Soil plays a key role in ecosphere and air quality regulation. Obsolete environmental technologies lead to soil quality loss, air, water, and land systems pollution. Pedosphere and plants are intertwined with the air quality. Ionized O2 is capable to intensify atmosphere turbulence, providing particulate matter (PM2.5) coalescence and dry deposition. Addressing environmental quality, a Biogeosystem Technique (BGT*) heuristic transcendental (nonstandard and not direct imitation of nature) methodology has been developed. A BGT* main focus is an enrichment of Earth's biogeochemical cycles through land use and air cleaning. An intra-soil processing, which provides the soil multilevel architecture, is one of the BGT* ingredients. A next BGT* implementation is intra-soil pulse continuously discrete watering for optimal soil water regime and freshwater saving up to 10-20 times. The BGT* comprises intra-soil dispersed environmentally safe recycling of the PM sediments, heavy metals (HMs) and other pollutants, controlling biofilm-mediated microbial community interactions in the soil. This provides abundant biogeochemical cycle formation and better functioning of the humic substances, biological preparation, and microbial biofilms as a soil-biological starter, ensuring priority plants and trees nutrition, growth and resistance to phytopathogens. A higher underground and aboveground soil biological product increases a reversible C biological sequestration from the atmosphere. An additional light O2 ions photosynthetic production ensures a PM2.5 and PM0.1 coalescence and strengthens an intra-soil transformation of PM sediments into nutrients and improves atmosphere quality. The BGT* provides PM and HMs intra-soil passivation, increases soil biological productivity, stabilizes a climate system of the earth and promotes a green circular economy.
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Poluição do Ar , Metais Pesados , Solo , Poluição do Ar/análise , Metais Pesados/análise , Material Particulado/análise , Plantas , ÁguaRESUMO
The use of surfactants in households and industries is inevitable and so is their discharge into the environment, especially into the water bodies as effluents. Being surface-active agents, their utilization is mostly seen in soaps, detergents, personal care products, emulsifiers, wetting agents, etc. Anionic surfactants are the most used class. These surfactants are responsible for the foam and froth in the water bodies and cause potential adverse effects to both biotic and abiotic components of the ecosystem. Surfactants are capable of penetrating the cell membrane and thus cause toxicity to living organisms. Accumulation of these compounds has been known to cause significant gill damage and loss of sight in fish. Alteration of physiological and biochemical parameters of water decreases the amount of dissolved oxygen and thus affecting the entire ecosystem. Microbes utilizing surfactants as substrates for energy form the basis of the biodegradation of these compounds. The main organisms for surfactant biodegradation, both in sewage and natural waters, are bacteria. Several Pseudomonas and Bacillus spp. have shown efficient degradation of anionic surfactants namely: sodium dodecyl sulphate (SDS), linear alkylbenzene sulphonate (LAS), sodium dodecylbenzenesulphonate (SDBS). Also, several microbial consortia constituting Alcaligenes spp., Citrobacter spp., etc. have shown efficacy in the degradation of surfactants. The biodegradation efficiency studies of these microbes/microbial consortia would be of immense help in formulating better solutions for the bioremediation of surfactants and help to reduce their potential environmental hazards.
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Ecossistema , Tensoativos , Animais , Bactérias/metabolismo , Biodegradação Ambiental , Tensoativos/metabolismo , Água/metabolismoRESUMO
Currently, the applications of biochar (BC) in agricultural practices and for environmental remediation purposes have demonstrated multifaceted advantages despite a few limitations. Nano-BC offers considerable opportunities especially for the remediation of hazardous contaminants as well as the improvement of crop productivity. Positive outcomes of nano-BC on soil physico-chemical and biological characteristics have indicated its suitability for agricultural applications. Nano-BC may effectively regulate the mobilization and sorption of important micro- and macro-nutrients, along with the hazardous contaminants including potentially toxic metals, pesticides, etc. Additionally, the sorption characteristics of nano-BC depends substantially on feedstock materials and pyrolysis temperatures. Nevertheless, the conducted investigations regarding nano-BC are in infant stages, requiring extensive field investigations. The nano-enhanced properties of BC on one hand dramatically improve its effectiveness and sustainability, on the other hand, there may be associated with toxicity development in diverse aquatic and/or terrestrial environments. Therefore, risk assessment on soil organisms and its indirect impact on human health is another area of concern linked with the field application of nano-BC. The present review delineates the potentiality of nano-BC as an emerging sorbent for sustainable agriculture and environmental applications.
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Recuperação e Remediação Ambiental , Poluentes do Solo , Agricultura , Carvão Vegetal , Humanos , Solo/química , Poluentes do Solo/análiseRESUMO
Antibiotic resistance is one of the major growing concerns for public health. Conventional antibiotics act on a few predefined targets and, with time, several bacteria have developed resistance against a large number of antibiotics. The WHO has suggested that antibiotic resistance is at a crisis stage and identification of new antibiotics and targets could be the only approach to bridge the gap. Filamentous Temperature Sensitive-Mutant Z (Fts-Z) is one of the promising and less explored antibiotic targets. It is a highly conserved protein and plays a key role in bacterial cell division by introducing a cytokinetic Z-ring formation. In the present article, the potential of over 165 cyanobacterial compounds with reported antibiotic activity against the catalytic core domain in the Fts-Z protein of the Bacillus subtilis was studied. The identified cyanobacterial compounds were screened using the GLIDE module of Maestro v-2019-2 followed by 100-ns molecular dynamics (MD) simulation. Ranking of the potential compound was performed using dock score and MMGBSA based free energy. The study reported that the docking score of aphanorphine (-6.010 Kcalmol-1) and alpha-dimorphecolic acid (ADMA) (-6.574 Kcalmol-1) showed significant role with respect to the reported potential inhibitor PC190723 (-4.135 Kcalmol-1). A 100 ns MD simulation infers that Fts-Z ADMA complex has a stable conformation throughout the progress of the simulation. Both the compounds, i.e., ADMA and Aphanorphine, were further considered for In-vitro validation by performing anti-bacterial studies against B. subtilis by agar well diffusion method. The results obtained through In-vitro studies confirm that ADMA, a small molecule of cyanobacterial origin, is a potential compound with an antibacterial activity that may act by inhibiting the novel target Fts-Z and could be a great drug candidate for antibiotic development.
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Bacillus subtilis , Cianobactérias , Bacillus subtilis/genética , Bacillus subtilis/metabolismo , Proteínas de Bactérias/metabolismo , Cianobactérias/metabolismo , Proteínas do Citoesqueleto/metabolismo , Proteínas Mutantes/metabolismoRESUMO
Nowadays, the applications of nanomaterials (NMs) are becoming the edge over others and referred as one of the pillars of emerging science and technology. Thereby, a wide array of NMs have been developed along with the products that can be used for the reclamation of contaminated terrestrial ecosystems. The NMs got a great consideration due to their peculiar characteristics and high efficacy. Therefore, this review addresses in depth the ability of metal(loid) NMs as nanosorbents along with their applications in soil remediation. Adsorption is commonly employed for the elimination of innumerable contaminants because of low expenses, reliability, and convenience. The first emphasis of this work will be the use of nanoscale meta(loid) adsorbents for contaminated soil remediation along with their geochemistry. Because NMs mediated soil remediation promises more efficient and cost-effective than conventional methods and can enhance the probability of in situ contaminants remediation. However, the extensive usage of NMs is enhancing their concentrations in the environment and get a route to enter the surrounding flora and fauna that can induce serious concerns due to the lack of absolute understanding regarding NMs interactions with living organisms. Therefore, the second focus of this work will be on the ecotoxicological impacts with special attentions on morpho-physiological alterations in edible plants.
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Metais Pesados , Poluentes do Solo , Ecossistema , Metais Pesados/análise , Reprodutibilidade dos Testes , Solo , Poluentes do Solo/análise , Poluentes do Solo/toxicidadeRESUMO
This study investigated levels and sources of pollution and potential health risks associated with potentially toxic elements (PTEs) and polycyclic aromatic hydrocarbons (PAHs) in urban street dust collected from Tyumen city, a large transport centre with one of the highest motorization rates in Russia. Twenty street dust samples were collected from four grades of roads in five different land use areas. Research methods included measurements of physical and chemical properties of street dust, concentrations of 18 PTEs using inductively coupled plasma mass spectrometry, 12 PAHs using high-performance liquid chromatography, and statistical analysis of the data. Concentrations of Ni, Cr, Sb, and Mo, as well as medium and high molecular weight PAHs in urban street dust, were notably higher than in soils within the city, which indicates that transport is the main source of these elements. Concentrations of Cu, Cd, Pb, Zn, Mn, and As in street dust of Tyumen were lower compared to many large cities, while Cr, Ni, and Co were higher. Concentrations of PAH were comparable to other large nonindustrial cities. Total contamination of street dust by both PTEs and PAHs showed more robust relationships with the number of roadway lanes rather than land use. The estimated carcinogenic risks were low in 70% of samples and medium in 30% of samples. Noncarcinogenic risks were attributed to exposure to Co, Ni, V, and As. The total noncarcinogenic risk for adults was found to be negligible, while the risk was found to be moderate for children.