RESUMO
BACKGROUND: Accelerometers are valid, practical and reliable tools for the measurement of habitual physical activity (PA). Quantification of PA in horses is desirable for use in research and clinical settings. The objective of this study was to evaluate a triaxial accelerometer for objective measurement of PA in the horse by assessment of their practical utility and validity. Horses were recruited to establish both the optimal site of accelerometer attachment and questionnaire designed to explore owner acceptance. Validity and cut-off values were obtained by assessing PA at various gaits. Validation study- 20 horses wore the accelerometer while being filmed for 10 min each of rest, walking and trotting and 5 mins of canter work. Practical utility study- five horses wore accelerometers on polls and withers for 18 h; compliance and relative data losses were quantified. RESULTS: Accelerometry output differed significantly between the four PA levels (P < 0â¢001) for both wither and poll placement. For withers placement, ROC analyses found optimal sensitivity and specificity at a cut-off of <47 counts per minute (cpm) for rest (sensitivity 99.5 %, specificity 100 %), 967-2424 cpm for trotting (sensitivity 96.7 %, specificity 100 %) and ≥2425 cpm for cantering (sensitivity 96.0 %, specificity 97.0 %). Attachment at the poll resulted in optimal sensitivity and specificity at a cut-off of <707 counts per minute (cpm) for rest (sensitivity 97.5 %, specificity 99.6 %), 1546-2609 cpm for trotting (sensitivity 90.33 %, specificity 79.25 %) and ≥2610 cpm for cantering (sensitivity 100 %, specificity 100 %) In terms of practical utility, accelerometry was well tolerated and owner acceptance high. CONCLUSION: Accelerometry data correlated well with varying levels of in-hand equine activity. The use of accelerometers is a valid method for objective measurement of controlled PA in the horse.
Assuntos
Acelerometria/veterinária , Cavalos/fisiologia , Atividade Motora/fisiologia , Acelerometria/instrumentação , Acelerometria/métodos , AnimaisRESUMO
The 13C-octanoic acid breath test has been correlated significantly to radioscintigraphy for measurement of gastric emptying indices in healthy horses. The objective of this study was to investigate the validity of the test for measurement of equine delayed gastric emptying, prior to its potential clinical application for this purpose. A model of atropine-induced gastroparesis was used. Gastric emptying rate was measured twice in 8 horses using concurrent radioscintigraphy and/or breath test after treatment i.v. with either atropine (0.035 mg/kg bwt) or saline in randomised order. Analysis of both data sets demonstrated that the atropine treatment had caused a significant delay in gastric emptying rate. Paired breath test data showed an atropine-induced delay in gastric half-emptying time (t 1/2), with no overlap in the 99% CI range (P < 0.001). Significant correlations were found between scintigraphy and 13C-octanoic acid breath test for calculation of both t 1/2 (P < 0.01) and lag phase duration (P < 0.05) in the atropine-delayed emptying results. The mean (s.d.) bias in breath test t 1/2 when compared with scintigraphy was 1.78 (0.58) h. The results demonstrated that the 13C-octanoic acid breath test was an effective diagnostic modality for the measurement of equine delayed gastric emptying. The technique offers advantages to existing methods for clinical investigation, as it is noninvasive, not radioactive, quantitative and requires minimal equipment or training to perform.
Assuntos
Testes Respiratórios/métodos , Caprilatos , Esvaziamento Gástrico/fisiologia , Gastroparesia/veterinária , Doenças dos Cavalos/diagnóstico , Animais , Atropina/administração & dosagem , Atropina/farmacologia , Caprilatos/farmacocinética , Dióxido de Carbono/análise , Isótopos de Carbono , Esvaziamento Gástrico/efeitos dos fármacos , Gastroparesia/induzido quimicamente , Gastroparesia/diagnóstico , Doenças dos Cavalos/induzido quimicamente , Doenças dos Cavalos/diagnóstico por imagem , Cavalos , Injeções Intravenosas/veterinária , Parassimpatolíticos/administração & dosagem , Parassimpatolíticos/farmacologia , Valor Preditivo dos Testes , Cintilografia , Distribuição Aleatória , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Estômago/diagnóstico por imagemRESUMO
REASONS FOR PERFORMING STUDY: Disordered gastric motility may be a significant factor in the pathogenesis of many equine conditions. Although tests for liquid phase emptying rate have been validated in the horse, there are no effective tests for solid phase emptying measurement that can be performed routinely in the field. OBJECTIVES: The objective of this study was the assessment of a novel stable isotope technique, the 13C-octane acid breath test (13C-OABT), for the measurement of gastric emptying of solid ingesta, by direct comparison with the optimum method of gastric scintigraphy. METHODS: To facilitate dual measurement of gastric emptying, a test meal was used containing baked egg yolk labelled with both 13C-octanoic acid and 99mtechnetium sulphur colloid. Simultaneous, serial lateral gastric scintigraphs and expiratory breath samples were obtained in 12 healthy horses after voluntary ingestion of the test meal. Analysis of breath 13CO2:12CO2 ratio was performed by continuous flow isotope ratio mass spectrometry. Power regression was used to determine the gastric emptying coefficient, the gastric half-emptying time (t 1/2) and duration of the lag phase (tlag). RESULTS: Significant correlations (P < 0.001) were found between the 2 techniques for measurement of both t 1/2 and tlag. In addition, scintigraphic left t 1/2 was correlated significantly to breath test gastric emptying coefficient (P < 0.001). CONCLUSIONS: It was concluded that the 13C-octanoic acid breath test is a reliable diagnostic procedure to measure gastric emptying rate of solids in the horse. POTENTIAL RELEVANCE: Being safe, noninvasive and easy to perform, this test has potential value as both sensitive diagnostic modality and humane research tool for motility studies.
Assuntos
Testes Respiratórios/métodos , Caprilatos/metabolismo , Esvaziamento Gástrico , Cavalos/fisiologia , Estômago/diagnóstico por imagem , Animais , Dióxido de Carbono/metabolismo , Isótopos de Carbono , Feminino , Esvaziamento Gástrico/fisiologia , Doenças dos Cavalos/diagnóstico , Masculino , Cintilografia , Sensibilidade e Especificidade , Estômago/fisiologia , Coloide de Enxofre Marcado com Tecnécio Tc 99m , Fatores de TempoRESUMO
The aim of this study was to measure the effects of specific commonly used sedative protocols on equine solid phase gastric emptying rate, using the 13C-octanoic acid breath test (13C-OABT). The gastric emptying of a standard 13C-labelled test meal was measured once weekly in 8 mature horses over two 4 week treatment periods. Each horse acted as its own control. In treatment Period 1, saline (2 ml i.v.), xylazine (0.5 mg/kg i.v.), detomidine (0.01 mg/kg i.v.) or detomidine/butorphanol combination (0.01/0.02 mg/kg i.v.) was administered in randomised order after ingestion of the test meal. During treatment Period 2, test meal consumption was followed by saline, xylazine (1.0 mg/kg i.v.), or detomidine (0.03 mg/kg i.v.) administration, or preceded by acepromazine (0.05 mg/kg i.m.) in randomised order. The 13C:12C ratio of sequential expiratory breath samples was determined by isotope ratio mass spectrometry, and used to measure the gastric half-emptying time, t 1/2, and duration of the lag phase, t lag, for each of the 64 tests. In treatment Period 1, detomidine/butorphanol prolonged both t 1/2 and t lag with respect to xylazine 0.5 mg/kg and the saline control (P < 0.05). In Period 2, detomidine 0.03 mg/kg delayed each parameter with respect to saline, acepromazine and xylazine 1.0 mg/kg (P < 0.001). Xylazine 1.0 mg/kg also lengthened t lag relative to the saline control (P = 0.0004), but did not cause a significant change in t 1/2. Comparison of treatment periods showed that the inhibitory effect of detomidine on gastric emptying rate was dose related (P<0.05). These findings may have clinical significance for case selection when these agents are used for purposes of sedation and/or analgesia.
Assuntos
Esvaziamento Gástrico/efeitos dos fármacos , Hipnóticos e Sedativos/farmacologia , Acepromazina/farmacologia , Agonistas alfa-Adrenérgicos/farmacologia , Analgésicos/farmacologia , Animais , Testes Respiratórios , Butorfanol/farmacologia , Caprilatos/farmacocinética , Dióxido de Carbono/análise , Isótopos de Carbono , Relação Dose-Resposta a Droga , Esvaziamento Gástrico/fisiologia , Gastroparesia/veterinária , Cavalos , Imidazóis/farmacologia , Modelos Lineares , Xilazina/farmacologiaRESUMO
Gastric emptying is the process by which food is delivered to the small intestine at a rate and in a form that optimizes intestinal absorption of nutrients. The rate of gastric emptying is subject to alteration by physiological, pharmacological, and pathological conditions. Gastric emptying of solids is of greater clinical significance because disordered gastric emptying rarely is detectable in the liquid phase. Imaging techniques have the disadvantage of requiring restraint of the animal and access to expensive equipment. Radiographic methods require administration of test meals that are not similar to food. Scintigraphy is the gold standard method for assessment of gastric emptying but requires administration of a radioisotope. Magnetic resonance imaging has not yet been applied for assessment of gastric emptying in small animals. Ultrasonography is a potentially useful, but subjective, method for assessment of gastric emptying in dogs. Gastric tracer methods require insertion of gastric or intestinal cannulae and are rarely applied outside of the research laboratory. The paracetamol absorption test has been applied for assessment of liquid phase gastric emptying in the dog, but requires IV cannulation. The gastric emptying breath test is a noninvasive method for assessment of gastric emptying that has been applied in dogs and cats. This method can be carried out away from the veterinary hospital, but the effects of physiological and pathological abnormalities on the test are not known. Advances in technology will facilitate the development of reliable methods for assessment of gastric emptying in small animals.
Assuntos
Gatos/fisiologia , Cães/fisiologia , Esvaziamento Gástrico/fisiologia , Acetaminofen/farmacocinética , Animais , Bário , Testes Respiratórios , Radioisótopos de Carbono , Árvores de Decisões , Imageamento por Ressonância Magnética/veterinária , Radiografia/veterinária , Cintilografia/veterinária , Coloide de Enxofre Marcado com Tecnécio Tc 99m , Tomografia Computadorizada por Raios X/veterinária , Ultrassonografia/veterináriaRESUMO
The analysis of exhaled breath is a potentially useful method for application in veterinary diagnostics. Breath samples can be easily collected from animals by means of a face mask or collection chamber with minimal disturbance to the animal. After the administration of a 13C-labelled compound the recovery of 13C in breath can be used to investigate gastrointestinal and digestive functions. Exhaled hydrogen can be used to assess orocaecal transit time and malabsorption, and exhaled nitric oxide, carbon monoxide and pentane can be used to assess oxidative stress and inflammation. The analysis of compounds dissolved in the aqueous phase of breath (the exhaled breath condensate) can be used to assess airway inflammation. This review summarises the current status of breath analysis in veterinary medicine, and analyses its potential for assessing animal health and disease.
Assuntos
Testes Respiratórios/métodos , Gastroenteropatias/veterinária , Medicina Veterinária/tendências , Animais , Testes Respiratórios/instrumentação , Gastroenteropatias/diagnósticoRESUMO
REASONS FOR PERFORMING STUDY: Imidocarb dipropionate is the drug of choice for equine piroplasmosis but its administration causes severe colic and diarrhoea. An imidocarb protocol that reduces these effects is needed. OBJECTIVES: 1) Quantification of the effects of imidocarb dipropionate on equine orocaecal transit time (OCTT), with and without atropine or glycopyrrolate premedication and 2) investigation of an improved pretreatment regimen for imidocarb administration. HYPOTHESIS: Treatment with imidocarb dipropionate will result in colic and reduced OCTT as demonstrated by the lactose 13C-ureide breath test which will be ameliorated by premedication with either atropine or glycopyrrolate. METHODS: The effects of 3 drug therapies on OCTT were compared in 6 healthy horses in a randomised double-blind study vs. a saline control: 1) imidocarb dipropionate 2.4 mg/kg bwt administered intramuscularly (i.m.) with saline administered intravenously (i.v.; imidocarb/saline); 2) imidocarb dipropionate 2.4 mg/kg bwt administered i.m. with atropine 0.035 mg/kg bwt administered i.v. (imidocarb/atropine) and 3) imidocarb dipropionate 2.4 mg/kg bwt administered i.m. with glycopyrrolate 0.0025 mg/kg bwt administered i.v. (imidocarb/glycopyrrolate). The lactose 13C-ureide breath test was used to measure OCTT in each case and significance of treatment effect determined by a linear model analysis of variance. RESULTS: Imidocarb/atropine treatment caused an increase in OCTT (P < 0.05) whereas imidocarb/saline produced a nonsignificant decrease in OCTT. Imidocarb/saline caused colic and diarrhoea in 4 of 6 horses, which were not seen in any of the horses treated with imidocarb/atropine or imidocarb/glycopyrrolate or administered the saline control. Intestinal borborygmi were increased in imidocarb/saline and decreased in imidocarb/atropine treated horses, respectively. CONCLUSIONS: Imidocarb/saline treatment induced colic signs and a potential reduction in OCTT while imidocarb/atropine treatment increased OCTT significantly when compared with imidocarb/saline. Both atropine and glycopyrrolate premedication ameliorated the clinical gastrointestinal effects of imidocarb but atropine produced significant inhibition of gastric and/or small intestinal motility not detected with glycopyrrolate. Premedication with glycopyrrolate is recommended when using imidocarb for treatment of equine piroplasmosis.
Assuntos
Babesiose/veterinária , Motilidade Gastrointestinal/efeitos dos fármacos , Doenças dos Cavalos/parasitologia , Imidocarbo/farmacologia , Lactose/metabolismo , Ureia/análogos & derivados , Animais , Atropina/farmacocinética , Isótopos de Carbono , Interações Medicamentosas , Glicopirrolato/farmacocinética , Frequência Cardíaca/efeitos dos fármacos , Doenças dos Cavalos/tratamento farmacológico , Cavalos , Imidocarbo/farmacocinética , Respiração/efeitos dos fármacos , Ureia/metabolismoRESUMO
REASONS FOR PERFORMING STUDY: Validation of a reliable, noninvasive clinical test for quantification of equine orocaecal transit time (OCTT) is required. This would facilitate an evidence-based approach to investigation and treatment of equine small intestinal disorders. OBJECTIVES: 1) Comparison of the lactose (13) C-ureide breath test (LUBT) with the hydrogen breath test (H(2) BT) for OCTT measurement. 2) Identification of the characteristics of gastrointestinal microbial glycosylureide hydrolase activity in vitro. 3) Production of an optimised protocol for the LUBT for in vivo measurement of equine OCTT. HYPOTHESIS: Significant lactose (13) C-ureide ((13) C-LU) hydrolase activity is restricted to the large bowel. The rate of expiratory (13) CO(2) production after ingestion of the isotope will provide an indirect quantifiable measure of orocaecal transit rate. Requisite bacterial activity may be enhanced by a primer dose of unlabelled substrate as shown in Man. METHODS: Combined LUBT and H(2) BT were performed in 8 healthy individuals. Analysis of sequential end expiratory breath samples was used to calculate OCTT and results compared. Digestion of (13) C-LU was investigated in vitro using fresh faecal material or intestinal aliquots collected post mortem. Isotopic fermentation rate was measured by rate of appearance of (13) CO(2) . RESULTS: Peaks in expiratory (13) CO(2) occurred in all individuals after ingestion of the labelled test meal, whereas H(2) expiration was variable. Both faecal and intestinal microbial digestion of (13) C-LU were maximised by prior exposure to (12) C-LU. Induced bacterial glucoseureide hydrolase activity was significantly greater in the caecum than in the small intestine (n = 10, P<0.05). CONCLUSIONS: Significant (13) C-LU digestion is restricted to the equine large intestine under normal conditions, and is enhanced by prior exposure to (12) C-LU, making (13) C-LU a suitable noninvasive marker of equine OCTT. The LUBT is more reliable than the H(2) BT for measurement of equine OCTT.
Assuntos
Testes Respiratórios/métodos , Trânsito Gastrointestinal/fisiologia , Cavalos/fisiologia , Lactose/metabolismo , Ureia/análogos & derivados , Animais , Isótopos de Carbono , Ceco , Fermentação , Boca , Reprodutibilidade dos Testes , Fatores de Tempo , Ureia/metabolismoRESUMO
REASONS FOR PERFORMING STUDY: Application of the lactose (13) C-ureide breath test (LUBT) for measurement of equine orocaecal transit time (OCTT) has not been reported previously. The ability to assess OCTT noninvasively, and to investigate its relationship to gastric emptying rate and small intestinal transit, would be of both clinical and research value. OBJECTIVES: 1) Assessment of the LUBT in healthy horses, with comparison of induced versus noninduced test protocols. 2) Application of a new dual stable isotope breath test (lactose (13) C-ureide and (13) C-octanoic acid) for gastrointestinal transit measurement. HYPOTHESIS: The LUBT will allow quantification of equine OCTT, and test efficacy will be enhanced by prior administration of lactose (12) C-ureide as shown in vitro. The dual tracer breath test will permit simultaneous measurement of gastric emptying, OCTT and small bowel transit times. METHODS: Induced and noninduced LUBTs were performed in 3 healthy mature horses in randomised order using a standard test meal and protocol. Combined LUBT and (13) C-octanoic acid breath tests ((13) C-OABT) were performed in 4 individuals on 4 occasions at weekly intervals. Expiratory isotopic recovery was modelled to allow generation of gastric emptying data, small bowel transit times and caecal transit parameters. RESULTS: The induction protocol for the LUBT increased the rate and magnitude of expiratory (13) CO(2) significantly. Mean ± s.d. values for OCTT, caecal lag phase (t(lag) ) and caecal t(1/2) using the induced LUBT were 3.24 ± 0.65 h, 5.62 ± 1.22 h and 6.31 ± 1.21 h, respectively. Dual stable isotope tests resulted in the production of 2 discrete peaks in expiratory (13) CO(2) in 15/16 tests from which gastric t(1/2), OCTT and small bowel transit (SBT) parameters could be calculated. CONCLUSIONS: The induced LUBT provides a reliable noninvasive measure of equine OCTT and can be paired with the (13) C-OABT to provide further information about small intestinal motility.