RESUMO
The spectacular threat display of the savannah specialist Australo-Papuan frilled lizards has made them one of the world's most iconic reptiles. They are increasingly used as a model system for research in evolutionary biology and ecology but little is known of their population structure. Their distribution across northern Australia and southern New Guinea also provides an opportunity to examine biogeographic patterns as they relate to the large-scale movement of savannah habitat during the Plio/Pleistocene and the associated increase in aridity. We generated sequence data for one mitochondrial and four nuclear DNA loci (5052 base pairs) for 83 frilled lizards sampled throughout their range. We also quantified body proportion variation for 279 individuals. Phylogenetic analyses based on maximum likelihood and Bayesian species-tree methods revealed three shallow clades that replace each other across the monsoon tropics. We found the expected pattern of male biased sexual size dimorphism in both maximum body size and head size but there was no sexual dimorphism in overall body shape or in frill size, relative to head size, supporting the hypothesis that the frill is used primarily as a threat display rather than a sexual display. The genetic clades are broadly consistent with known clinal variation in frill color that gradually shifts from west to east (red, orange, yellow/white) but otherwise show little morphological differentiation in body proportion measures. The biogeographic breaks between clades occur at the Carpentaria Gap and the lowlands surrounding the Ord River, and our ecological niche modeling predicts lower habitat suitability for C. kingii in these regions. While this biogeographic pattern is consistent with numerous other taxonomic groups in northern Australia, the overall low genetic diversity in frilled lizards across the entire monsoon tropics and southern New Guinea contrasts starkly to patterns seen in other terrestrial vertebrates. Extremely low intra-clade genetic diversity over vast geographic areas is indicative of recent gene flow that would likely have been facilitated by widespread savannah during interglacials, or alternatively may reflect population bottlenecks induced by extreme aridity during Pleistocene glacials. The shallow divergence between Australian and New Guinean samples is consistent with recent connectivity between Australia and New Guinea that would have been possible via a savannah corridor across the Torres Strait. Based on our molecular and morphological data, we do not support taxonomic recognition of any of the frilled lizard clades and instead consider C. kingii a single species with shallow phylogeographic structure and clinal variation in frill color.
Assuntos
Lagartos/classificação , Animais , Austrália , Teorema de Bayes , Evolução Biológica , DNA/química , DNA/isolamento & purificação , DNA/metabolismo , DNA Mitocondrial/classificação , DNA Mitocondrial/genética , DNA Mitocondrial/metabolismo , Feminino , Fluxo Gênico , Variação Genética , Lagartos/anatomia & histologia , Lagartos/genética , Masculino , Nova Guiné , Filogenia , Filogeografia , Análise de Sequência de DNARESUMO
Regional coexistence of ecologically similar species is facilitated when fluctuations in environmental conditions favor different species at different times or places. However, why species with similar ecology should vary in their response to environmental change is unclear. In this study, we explore the role of a life-history divergence in causing changes in relative fitness across environmental conditions experienced by populations of two closely related Ficedula flycatchers on the Baltic island of Oland, Sweden. We compared patterns of nestling survival between Pied (Ficedula hypoleuca) and Collared (F. albicollis) Flycatchers in relation to two factors known to influence the environment experienced by nestlings: natural variation in their parents' onset of breeding and artificial manipulation of the brood size. Possible differences in the location of the nests (i.e., microhabitat differences) or in habitat use (i.e., feeding patterns) by the adult birds were controlled for by partial cross-fostering of young between the two species. We found that nestling mortality was relatively higher among Collared Flycatchers and that this difference increased with later breeding. Mass gain, which predicted survival probability, of nestling Collared Flycatchers did not respond to the seasonal decline in environmental conditions when they were raised in nests with reduced brood size (i.e., where sibling competition was experimentally reduced). This latter result suggests that the smaller clutch size of Collared Flycatchers reflects an adaptive adjustment to their offspring's higher sensitivity to environmental change. We discuss the possibility that the divergence in life-history traits between the two species represents adaptation to different environments experienced during their recent evolutionary history. We conclude that the survival of nestling Collared Flycatchers is more sensitive to harsh environment and that this is likely to limit where and when the more aggressive Collared Flycatchers are able to displace Pied Flycatchers. Our results provide support for models of species coexistence that emphasize the importance of spatial or temporal heterogeneity in relative fitness or life-history divergence. More precisely, our results demonstrate that variation in life-history adaptations may result in changes in relative fitness of species across environments despite their use of similar resources.
Assuntos
Ecossistema , Passeriformes/fisiologia , Adaptação Fisiológica , Animais , Demografia , Reprodução , Fatores de TempoRESUMO
While sexual selection is generally assumed to quickly cause or strengthen prezygotic barriers between sister species, its role in causing postzygotic isolation, through the unattractiveness of intermediate hybrids, is less often examined. Combining 24 years of pedigree data and recently developed species-specific molecular markers from collared (Ficedula albicollis) and pied (Ficedula hypoleuca) flycatchers and their hybrids, we were able to quantify all key components of fitness. To disentangle the relative role of natural and sexual selection acting on F1 hybrid flycatchers, we estimated various fitness components, which when combined represent the total lifetime reproductive success of F1 hybrids, and then compared the different fitness components of F1 hybrids to that of collared flycatchers. Female hybrid flycatchers are sterile, with natural selection being the selective force involved, but male hybrids mainly experienced a reduction in fitness through sexual selection (decreased pairing success and increased rate of being cuckolded). To disentangle the role of sexual selection against male hybrids from a possible effect of genetic incompatibility (on the rate of being cuckolded), we compared male hybrids with pure-bred males expressing intermediate plumage characters. Given that sexual selection against male hybrids is a result of their intermediate plumage, we expect these two groups of males to have a similar fitness reduction. Alternatively, hybrids have reduced fitness owing to genetic incompatibility, in which case their fitness should be lower than that of the intermediate pure-bred males. We conclude that sexual selection against male hybrids accounts for approximately 75% of the reduction in their fitness. We discuss how natural and sexual selection against hybrids may have different implications for speciation and conclude that reinforcement of reproductive barriers may be more likely when there is sexual selection against hybrids.
Assuntos
Hibridização Genética , Passeriformes/fisiologia , Reprodução/fisiologia , Seleção Genética , Animais , Feminino , Masculino , Passeriformes/anatomia & histologia , Fenótipo , Análise de Componente PrincipalRESUMO
Conflicts over the delivery and sharing of food among family members are expected to lead to evolution of exaggerated offspring begging for food. Coevolution between offspring begging intensity and parent response depends on the genetic architecture of the traits involved. Given a genetic correlation between offspring begging intensity and parental response, there may be fast and arbitrary divergence in these behaviours between populations. However, there is limited knowledge about the genetic basis of offspring solicitation and parental response and whether these traits are genetically correlated. In this study, we performed a partial cross-fostering experiment of young between pied and collared flycatchers (Ficedula hypoleuca and Ficedula albicollis) and recorded the behaviour of individual offspring and their (foster)parents. We found that nestling collared flycatchers reached a higher phenotypic quality, estimated both as mass at fledging and as intensity of their T-lymphocyte-mediated immune response when raised by heterospecific foster parents. However, although collared flycatchers begged relatively more intensively, we found no evidence of corresponding higher resistance (i.e. lower feeding rate) of adult collared flycatchers than of adult pied flycatchers. Thus, the difference in offspring begging intensity between the two species seems not to be a result of a difference in escalation of the parent-offspring conflict. Instead, the species' divergence in exaggeration of offspring begging intensity 'honestly' matches a difference between the species in offspring need. This interpretation is strengthened by the fact that the difference in begging intensity between the two species increased as the season progressed, coinciding with the higher sensitivity of nestling collared flycatchers to the seasonal decline in food availability. Thus, the behavioural differentiation appears to be a direct consequence of a life-history differentiation (offspring growth patterns).
Assuntos
Evolução Biológica , Comportamento Alimentar/fisiologia , Comportamento de Nidação/fisiologia , Fenótipo , Aves Canoras/fisiologia , Análise de Variância , Animais , Aves Canoras/genética , Aves Canoras/imunologia , Especificidade da Espécie , SuéciaRESUMO
In the face of hybridization, species integrity can only be maintained through post-zygotic isolating barriers (PIBs). PIBs need not only be intrinsic (i.e. hybrid inviability and sterility caused by developmental incompatibilities), but also can be extrinsic due to the hybrid's intermediate phenotype falling between the parental niches. For example, in migratory species, hybrid fitness might be reduced as a result of intermediate migration pathways and reaching suboptimal wintering grounds. Here, we test this idea by comparing the juvenile to adult survival probabilities as well as the wintering grounds of pied flycatchers (Ficedula hypoleuca), collared flycatchers (Ficedula albicollis) and their hybrids using stable isotope ratios of carbon (delta13C) and nitrogen (delta15N) in feathers developed at the wintering site. Our result supports earlier observations of largely segregated wintering grounds of the two parental species. The isotope signature of hybrids clustered with that of pied flycatchers. We argue that this pattern can explain the high annual survival of hybrid flycatchers. Hence, dominant expression of the traits of one of the parental species in hybrids may substantially reduce the ecological costs of hybridization.
Assuntos
Migração Animal , Demografia , Genética Populacional , Hibridização Genética , Passeriformes/genética , África , Fatores Etários , Análise de Variância , Animais , Isótopos de Carbono/análise , Análise por Conglomerados , Padrões de Herança/genética , Isótopos de Nitrogênio/análise , Passeriformes/fisiologia , Polimorfismo de Nucleotídeo Único , Estações do AnoRESUMO
Quantifying differences in aggressive behaviour across contexts can be useful in developing an understanding of life histories and breeding systems, as well as the relative costs and benefits of such behaviour. We investigated whether age, relative body size and colouration, sex, and breeding stage influenced levels of aggressive behaviour in two contexts, towards conspecific and heterospecific intruders (mounts) around active nests of group living Crimson Finches (Neochmia phaeton). We found that when responding to a conspecific mount, relative body size, and age were important in predicting the aggressive response of males toward a conspecific, with older males and those close in size to their opponent showing a higher aggressive response. On the other hand, factors relating to female aggression were not as clear. In contrast, response to a heterospecific mount was unrelated to age, colour or size in either sex. Additionally, although birds were equally aggressive to conspecific and heterospecific mount types, we found no evidence that individuals are consistent in their level of aggression across these contexts. This suggests that aggressive behaviour in Crimson Finches is at least partially plastic and that individuals may be capable of assessing and responding to situations independently.
Assuntos
Agressão , Tentilhões , Comportamento Sexual Animal , Fatores Etários , Animais , Tamanho Corporal , Feminino , Masculino , Pigmentação , Caracteres SexuaisRESUMO
In many species, individuals do not attain their full adult coloration until one or several years after reaching sexual maturity, and this signaling of juvenile status is thought to enable young individuals to avoid aggression from older, dominant conspecifics. We propose that hybridization may be one of several costs and benefits associated with such delayed maturation. We tested this idea in a hybrid zone of collared (Ficedula albicollis) and pied (F. hypoleuca) flycatchers on the Baltic islands of Oland and Gotland. One-year-old (subadult) male collared flycatchers differed from older birds in many plumage traits, and approached male pied flycatchers in phenotype. On both islands, subadult male collared flycatchers hybridized at a higher rate than adults. Mate-choice experiments in aviaries suggest that this difference is at least partly due to female pied flycatchers having a preference for subadults when constrained to choose a heterospecific mate. Because novel morphologies are often derived from changes in ontology, juvenile forms may resemble adults of closely related taxa. When such juveniles are reproductively mature, their phenotypic similarity to the adults of closely related species may increase their risk of hybridization.
Assuntos
Hibridização Genética , Caracteres Sexuais , Aves Canoras , Fatores Etários , Animais , Feminino , Masculino , Comportamento Sexual AnimalRESUMO
The effect of separation by biogeographic features followed by secondary contact can blur taxonomic boundaries and produce complex genetic signatures. We analyzed population structure and gene flow across the range of the long-tailed finch (Poephila acuticauda) in northern Australia (1) to test the hypothesis that Ord Arid Intrusion acted as the causative barrier that led to divergence of P. acuticauda subspecies, (2) to determine whether genetic data support the presence of a gradual cline across the range or a sudden shift, both of which have been suggested based on morphological data, and (3) to estimate levels of contemporary gene flow within this species complex. We collected samples from 302 individuals from 10 localities. Analyses of 12 microsatellite loci and sequence data from 333 base pairs of the mitochondrial control region were used to estimate population structure and gene flow, using analysis of molecular variance (AMOVA), haplotype network analysis, frequency statistics, and clustering methods. Mitochondrial sequence data indicated the presence of three genetic groups (regions) across the range of P. acuticauda. Genetic diversity was highest in the east and lowest in the west. The Ord Arid Intrusion appears to have functioned as a biogeographic barrier in the past, according to mtDNA evidence presented here and evidence from previous studies. The absence of isolation by distance between adjacent regions and the lack of population genetic structure of mtDNA within regions indicates that genetic changes across the range of P. acuticauda subspecies are characterized by discrete breaks between regions. While microsatellite data indicate a complete absence of genetic structure across this species' range, it appears unlikely that this results from high levels of gene flow. Mitochondrial data do not support the presence of contemporary gene flow across the range of this species.
RESUMO
Interbreeding between species (hybridization) typically produces unfit offspring. Reduced hybridization should therefore be favored by natural selection. However, this is difficult to accomplish because hybridization also sets the stage for genetic recombination to dissociate species-specific traits from the preferences for them. Here we show that this association is maintained by physical linkage (on the same chromosome) in two hybridizing Ficedula flycatchers. By analyzing the mating patterns of female hybrids and cross-fostered offspring, we demonstrate that species recognition is inherited on the Z chromosome, which is also the known location of species-specific male plumage traits and genes causing low hybrid fitness. Limited recombination on the Z chromosome maintains associations of Z-linked genes despite hybridization, suggesting that the sex chromosomes may be a hotspot for adaptive speciation.
Assuntos
Evolução Biológica , Ligação Genética , Especiação Genética , Preferência de Acasalamento Animal , Cromossomos Sexuais/genética , Aves Canoras/fisiologia , Animais , Plumas , Feminino , Fluxo Gênico , Hibridização Genética , Masculino , Recombinação Genética , Reprodução , Comportamento Sexual Animal , Aves Canoras/anatomia & histologia , Aves Canoras/genéticaRESUMO
Spatial and temporal heterogeneity in relative fitness of competing species is a key factor affecting the structure of communities. However, it is not intuitive why species that are ecologically similar should differ in their response to environmental changes. Here we show that two sympatric flycatchers differ in reproductive strategy and in sensitivity to harsh environment. The fitness of collared flycatchers (Ficedula albicollis), which are dominant in interference competition, is more sensitive than the fitness of pied flycatchers (Ficedula hypoleuca) to the seasonal decline in environmental conditions. In order to control for the possibility that this pattern arises solely from differences in microhabitat use (i.e. a local niche differentiation), we performed a partial cross-fostering experiment of young between the two species (i.e. resulting in nests containing young of both species). Our results show that the growth of nestling pied flycatchers is less influenced by the seasonal decline in environmental conditions. We suggest that a life-history trade-off between interference competitive ability and robustness to harsh environment promotes a regional coexistence of the two species.