[The effect of orthofen (diclofenac) on the embryogenesis of laboratory mice]. / Vliianie ortofena (diklofenaka) na émbriogenez laboratornykh myshei.

The effect of ortophen on the embryogenesis of laboratory mice of two inbred lines BALB/c and C57B1/6 and of outbred stock NMR was studied. The drug was injected intraperitoneally on days 1-6, 6-16, and 16-18 of pregnancy in a therapeutic dose (1 mg/g) and 10-fold doses. General development of the fetuses was delayed and the condition of the pregnant females of both lines and the stock deteriorated on injection of the drug in a 10-fold dose on days 16-18 of pregnancy. BALB/c mice proved to be most sensitive to the unfavorable effects of sodium diclophenac.

[Nonviral gene transfer in vivo in gene therapy]. / Nevirusnyi perenos genov in vivo v gennoi terapii.

Recent findings connected with in vivo use of artificial macromolecular complexes (genosomes) for functional gene transfer and delivery are discussed in the paper. Non-viral methods are the most safe for the purpose of human gene delivery. The cationic liposomes containing cholesterol are the most suitable for this purpose, because they possess high biodegradability and stability in blood stream. The DNA-liposome complexes should: (i) contain DNA in the condition at most protected from environmental influence, (ii) be rather homogeneous and of small size (40-80 nm). Injections of complexes into blood are the most effective; in a respect of organospecifity may be achieved by appropriate ligand selection. It is the most perspectively to increase the expression level by combining liposomes with viral peptides.