RESUMO
Vaclav Trnka from Krovice (1739-1791, in Latin: Wenzel Trnka Krzowitz) was a remarkable physician whose life serves as an example in the history of medicine by connecting major capital cities of Central Europe. In view of current geographical layout, he was born and brought up in the Czech Republic, graduated from University of Vienna in Austria, and was appointed Professor of the Anatomy at the newly established Faculty of Medicine of University of Nagyszombat, presently Trnava in Slovak Republic. When the University moved to Buda and later to Pest (today Budapest, Hungary), he was the first educator to introduce anatomy as a medical subject to be taught in a Hungarian medical school. He also was elected the Dean of Faculty of Medicine three times and in 1786-1787 he acted as Rector of then the Royal University of Pest. During his life, he published twenty-seven monographs dealing with different areas of clinical medicine, such as malaria (intermittent fever), diabetes, and rickets. Based on these monographs we can proclaim that Václav Trnka was a co-founder of modern infectology, diabetology and ophthalmology in Central Europe. Nowadays, artificial intelligence and bioinformatics are inseparable parts of modern health care system which help the transformation of big data into valuable knowledge. In the 18th century, Professor Trnka owned more than 3,000 scientific books and had natural, innate intelligence and wisdom which made him a real "medical polymath". As a musician, Trnka also composed sixty-one canons, two of them long wrongly considered as Mozart's work. Despite the fact that Trnka is considered to be the founder of Hungarian anatomy education and a major medical figure of the eighteenth century Central Europe, no internationally acclaimed biographical record of his life or work has so far been published in English. Therefore, we would like to reintroduce Václav Trnka both as an anatomist and medical polymath, and to give an overview of the early days of anatomy teaching in present-day Slovakia and Hungary (Fig. 1, Ref. 27). Keywords: Trnka from Krovice, anatomist, medical polymath, history of medicine.
Assuntos
Anatomistas , Anatomistas/história , República Tcheca , Europa (Continente) , História do Século XVIII , Humanos , Hungria , EslováquiaRESUMO
OBJECTIVE: The aim of the present study was to investigate the possible microvascular regulatory role of vascular endothelial growth factor receptor type 2 (VEGFR2) in experimental gingivitis in rats. BACKGROUND: Our previous results demonstrated that functionally active VEGFR2s are located in the venules of rat gingiva. While there is no remarkable endogenous gingival VEGF production under normal circumstances, exogenous VEGF, via VEGFR2, shows venodilatory effects. We assumed that VEGF plays an important role in vasoregulatory processes (vasodilation, increased permeability, angiogenesis) of gingival inflammation. METHODS: Gingivitis was induced by placing ligatures and composite material around and between the lower incisors of anesthetized Wistar rats next to the gingival margin. Seven days later, VEGFR2 antagonist (ZM323881), was dripped upon the labial gingiva next to the lower incisors. Diameter changes of the selected gingival venules were measured by vital microscopy. Animals with healthy gingiva served as controls. Venule diameter changes were compared to the baseline and to control groups (no ligature). Immunohistochemical and Western blot analysis for VEGFR2 were utilized. RESULTS: After 15, 30 and 60 min of local application of ZM323881, there was a significant venoconstriction in the inflamed gingiva compared to the baseline, while no change was recorded in controls. Endothelium, smooth muscle cells and pericytes of the gingivitis group showed increased VEGFR2 expression. CONCLUSION: Our findings suggest that there is an increased VEGF production in gingivitis, which may play an important role in vasodilation of rat gingival venules.
Assuntos
Gengivite/patologia , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/análise , Vênulas/patologia , Animais , Permeabilidade Capilar/efeitos dos fármacos , Endotélio Vascular/efeitos dos fármacos , Endotélio Vascular/patologia , Gengiva/irrigação sanguínea , Masculino , Músculo Liso Vascular/efeitos dos fármacos , Músculo Liso Vascular/patologia , Neovascularização Patológica/induzido quimicamente , Pericitos/efeitos dos fármacos , Pericitos/patologia , Quinazolinas/farmacologia , Distribuição Aleatória , Ratos , Ratos Wistar , Fatores de Tempo , Fator A de Crescimento do Endotélio Vascular/farmacologia , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/antagonistas & inibidores , Receptor 2 de Fatores de Crescimento do Endotélio Vascular/fisiologia , Vasoconstritores/farmacologia , Vasodilatadores/farmacologia , Vênulas/efeitos dos fármacos , Vênulas/fisiologiaRESUMO
BACKGROUND: NT-proXNP, a new natriuretic peptide analyte, incorporates information about the concentrations of both N-terminal pro-atrial and pro-brain natriuretic peptides (NT-proANP, NT-proBNP). We aimed to investigate whether NT-proXNP is a reliable indicator of the cardiac index (CI) and the hemodynamic state in neonates and infants undergoing an open heart surgery. METHODS: We enrolled 26 children under the age of 1 year into this prospective study. All patients underwent an elective cardiac operation with cardiopulmonary bypass (CPB) to achieve complete biventricular repair. Peri-operative hemodynamic parameters were assessed by transpulmonary thermodilution and natriuretic peptide levels were recorded. RESULTS: The NT-proXNP level correlated significantly with the simultaneously measured NT-proANP level (r=0.60, P<0.001), but more strongly with the NT-proBNP level (r=0.89, P<0.001) and the arithmetic sum of both (r=0.88, P<0.001). NT-proXNP had a strong correlation with CI (r=-0.85, P<0.001), the stroke volume index (r=-0.80, P<0.001) and the global ejection fraction (r=-0.67, P<0.009) throughout the post-operative period. Conventionally measured parameters such as heart rate, mean arterial pressure and pulse-pressure product exhibited weaker correlations with CI than NT-proXNP. Among laboratory values, creatinine levels correlated significantly with CI (r=-0.77, P<0.001) and NT-proXNP (r=0.76, P<0.001) during the post-operative period. A post-operative NT-proXNP level of 3079 pmol/l was diagnostic for CI <3 l/min/m(2) with 89% sensitivity and 90% specificity (area under the curve: 0.91 +/- 0.05). CONCLUSION: NT-proXNP is a good marker of cardiac output following pediatric cardiac surgery and might be a useful tool in the recognition of a low output state.
Assuntos
Débito Cardíaco/fisiologia , Procedimentos Cirúrgicos Cardíacos , DNA Helicases/metabolismo , Proteínas Nucleares/metabolismo , Biomarcadores , Creatinina/sangue , Eletrocardiografia , Feminino , Cardiopatias Congênitas/cirurgia , Frequência Cardíaca/fisiologia , Ventrículos do Coração/cirurgia , Hemodinâmica/fisiologia , Humanos , Lactente , Recém-Nascido , Masculino , Período Pós-Operatório , Estudos Prospectivos , Precursores de Proteínas/metabolismo , Volume Sistólico/fisiologia , Termodiluição , Proteína Nuclear Ligada ao XRESUMO
A variable number of tandem repeats (VNTR) polymorphism in exon 3 of the human dopamine D4 receptor gene (DRD4) has been associated with attention deficit hyperactivity disorder (ADHD). Rodents possess no analogous repeat sequence, whereas a similar tandem repeat polymorphism of the DRD4 gene was identified in dogs, horses and chimpanzees. Here, we present a genetic association study of the DRD4 VNTR and the activity-impulsivity dimension of the recently validated dog-ADHD Rating Scale. To avoid false positives arising from population stratification, a single breed of dogs (German shepherd) was studied. Two DRD4 alleles (referred to as 2 and 3a) were detected in this breed, and genotype frequencies were in Hardy-Weinberg equilibrium. For modelling distinct environmental conditions, 'pet' and 'police' German shepherds were characterized. Police German shepherds possessing at least one 3a allele showed significantly higher scores in the activity-impulsivity dimension of the dog-ADHD Rating Scale than dogs without this allele (P = 0.0180). This difference was not significant in pet German shepherds. To the best of our knowledge, this is the first report of an association between a candidate gene and a behaviour trait in dogs, and it reinforces the functional role of DRD4 exon 3 polymorphism.
Assuntos
Comportamento Animal , Cães/genética , Comportamento Impulsivo/genética , Polimorfismo Genético , Receptores de Dopamina D4/genética , Agressão , Animais , Cães/fisiologia , Frequência do Gene , Genótipo , FenótipoRESUMO
BACKGROUND: Catheter ablation is a proven therapy of focal atrial tachycardia. However limited information is available about the additional value of electroanatomical over conventional mapping methods for this specific arrhythmia. METHODS: Consecutive catheter ablation procedures of FAT were analyzed in two cardiology centres. Only conventional mapping was used in 30 of the 60 procedures whereas additionally CARTO mapping was performed in another 30 procedures. Acute, six-month success rate, and procedural data were analyzed. RESULTS: Localization of ectopic foci is congruent with previously published data. There was no statistically significant difference between procedure time and fluoroscopy time using additionally CARTO mapping, compared to conventional mapping only. Acute success rate was higher in procedures guided by CARTO mapping than in procedures based on conventional mapping (27/30 vs. 18/30, p = 0.0081). During the 6-month follow-up period there was a better outcome (p = 0.045) in case of CARTO guided procedures (success: 11 cases, partial success: 12 cases, failure: 4 cases) compared to conventional mapping (success: 4 cases, partial success: 18 cases, failure: 7 cases). CONCLUSIONS: Catheter ablation of focal atrial tachycardias using the CARTO electroanatomical mapping system seems to provide higher acute and 6-month success rate compared to ablation using conventional mapping methods only.
Assuntos
Ablação por Cateter/métodos , Técnicas Eletrofisiológicas Cardíacas , Átrios do Coração/cirurgia , Taquicardia Atrial Ectópica/cirurgia , Potenciais de Ação , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Átrios do Coração/fisiopatologia , Humanos , Hungria , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Recidiva , Estudos Retrospectivos , Fatores de Risco , Taquicardia Atrial Ectópica/diagnóstico , Taquicardia Atrial Ectópica/fisiopatologia , Fatores de Tempo , Resultado do Tratamento , Adulto JovemRESUMO
The avian hippocampal formation (HP) is considered to be homologous to the mammalian hippocampus, being involved in memory formation and spatial memory in particular. The subdivisions and boundaries of the pigeon hippocampus have been defined previously by various morphological methods to detect further similarities with the mammalian homologue. We studied the efferent projections of the zebra finch hippocampus by applying Phaseolus vulgaris leucoagglutinin, and three main subdivisions were distinguished on the basis of the connectivity patterns. Dorsolateral injections gave rise to projections innervating the rostralmost extension of the HP, a laminar complex including the dorsal and ventral hyperstriata and the lamina frontalis superior, the rostral lobus parolfactorius, the medial and ventral paleostriatal regions, the lateral septal nucleus, the nucleus of the diagonal band, the dorsolateral corticoid area, the archistriatum posterius, and the nucleus taeniae in the telencephalon. In the diencephalon, labelled axons were seen in the periventricular and lateral hypothalamus, including the lateral mammillary nuclei, and in the dorsolateral and the dorsomedial posterior thalamic nuclei, whereas, in the midbrain, only the area ventralis of Tsai contained hippocampal fibres. With the exception of the bilateral archistriatal efferents, all projections were ipsilateral. Dorsomedial injections gave rise to a local fibre system that was almost completely restricted to the ipsilateral hippocampal formation. In addition, lectin-containing fibres continued in the dorsal septal region and a thin band in the hyperstriatum accessorium, adjacent to the lateral ventricle. Ventral injections gave rise to axons innervating ipsilaterally the dorsolateral subdivision, and bilaterally the medial septal nuclei and the contralateral ventral hippocampus.
Assuntos
Vias Eferentes/anatomia & histologia , Hipocampo/anatomia & histologia , Animais , Aves , Feminino , Histocitoquímica , MasculinoRESUMO
The present study is the first comprehensive mapping of glial fibrillary acidic protein (GFAP)-immunopositive structures in the avian brain. Two main types of GFAP-immunopositive elements were observed: (1) nonbranching fibers, occasionally twisted or varicose, and (2) star-shaped cells. Long immunostained fibers originate from the lateral ventricle to form three bundles. Fibers of the dorsal group, emanating from the dorsal/lateral corner of the ventricle, course in lateral, anterior, and ventral directions forming a semidome, which separates the outer pallial (lateral cortical) regions from the underlying striatal mass. The middle group of fibers is directed anteriorly and laterally corresponding to the laminae frontales superior and suprema. The ventral fiber bundle is conical and traverses the lobus parolfactorius, crossing also the lamina medullaris dorsalis (the latter consisting mainly of star-shaped cells). The hippocampus, septum, and hypothalamus also contain straight radial fibers. In some areas, given their variable orientation, the fibers cannot be regarded as merely persisting radial glia. In the telencephalon, the nuclei basalis, accumbens, ectostriatum, paleostriatum primitivum, and the ventral paleostriatum are particularly rich in GFAP-positive cells, whereas the neostriatum, hyperstriatum, and paleostriatum augmentatum are almost devoid of GFAP labelling. Certain nuclei of the thalamus and the lower brainstem are conspicuous by their low levels of GFAP immunoreactivity. The Bergmann glia were GFAP-immunonegative.
Assuntos
Química Encefálica/fisiologia , Encéfalo/anatomia & histologia , Galinhas/anatomia & histologia , Proteína Glial Fibrilar Ácida/metabolismo , Animais , Encéfalo/citologia , Encéfalo/fisiologia , Mapeamento Encefálico , Tronco Encefálico/anatomia & histologia , Tronco Encefálico/fisiologia , Galinhas/metabolismo , Feminino , Proteína Glial Fibrilar Ácida/imunologia , Imuno-Histoquímica , Masculino , Fibras Nervosas/imunologia , Fibras Nervosas/metabolismo , Neuroglia/fisiologia , Telencéfalo/anatomia & histologia , Telencéfalo/fisiologiaRESUMO
The chick archistriatum receives afferents from the intermediate part of the medial hyperstriatum ventrale (IMHV) and projects to the lobus parolfactorius (LPO). There is functional evidence to suggest that the IMHV and the LPO are connected, but there is no anatomical evidence for a direct connection between the two structures. The aim of the current study was to characterize the termination pattern of medial hyperstriatal afferents within the archistriatum to determine whether the archistriatum may act as a relay between the IMHV and LPO. Following iontophoresis of Phaseolus vulgaris leucoagglutinin into the medial hyperstriatum ventrale (including the IMHV) of 1-week-old domestic chicks, anterogradely labelled fibers were observed to descend through the medial neostriatum and paleostriatum to enter the archistriatum. These medial hyperstriatum ventrale afferents arborised profusely to give varicose axon branches within all except the anterior part of the archistriatum. However, the greatest density was present in the ventral part of the intermediate archistriatum. Electron microscope examination of Phaseolus lectin immunocytochemistry and Golgi impregnation revealed that medial hyperstriatum ventrale axons formed multiple asymmetric synapses with dendritic spines (head and neck regions) on the terminal and preterminal dendritic segments of densely spiny archistriatal projection neurons. Medial hyperstriatum ventrale afferents were not observed to contact calbindin immunoreactive, presumptive "local circuit" neurons, within the archistriatum, despite a spatial overlap in their distribution. These results suggest that the archistriatum may be capable of mediating the transfer of information from the IMHV to the LPO.
Assuntos
Gânglios da Base/citologia , Galinhas/anatomia & histologia , Neostriado/citologia , Neurônios Eferentes/fisiologia , Animais , Axônios/fisiologia , Axônios/ultraestrutura , Gânglios da Base/fisiologia , Gânglios da Base/ultraestrutura , Calbindinas , Dendritos/fisiologia , Dendritos/ultraestrutura , Vias Eferentes/citologia , Vias Eferentes/fisiologia , Vias Eferentes/ultraestrutura , Feminino , Imuno-Histoquímica , Masculino , Microscopia Eletrônica , Neostriado/fisiologia , Neostriado/ultraestrutura , Fibras Nervosas/fisiologia , Fibras Nervosas/ultraestrutura , Proteínas do Tecido Nervoso/metabolismo , Neurônios Eferentes/ultraestrutura , Fito-Hemaglutininas , Proteína G de Ligação ao Cálcio S100/metabolismo , Coloração e RotulagemRESUMO
In 1-week-old domestic chicks, the connectivity of the lobus parolfactorius (LPO), part of the avian basal ganglia, was investigated using Phaseolus vulgaris leucoagglutinin and horseradish peroxidase for anterograde and retrograde pathway tracing, respectively. Tyrosine hydroxylase immunocytochemistry was applied in combination with Phaseolus lectin to assess the overlap between LPO efferents and diencephalic and mesencephalic catecholamine centres. Anterograde projections from LPO were detected in the hyperstriatum, neostriatum, and paleostriatum. Intranuclear connections were also apparent within the LPO. Descending LPO efferents innervated the lateral mammillary and intramedial nuclei and the dorsomedial thalamic complex. Fibres from LPO were observed in the tectal gray, substantia nigra, area ventralis tegmentalis of Tsai, and the adjacent nucleus mesencephalicus profundus. Further caudally, projections from LPO reached the nucleus papillioformis, locus coeruleus, and subcoeruleus ventralis. LPO efferents were coextensive with tyrosine hydroxylase-positive cells in the nuclei mamillaris lateralis and intramedialis of the hypothalamus, area ventralis tegmentalis, substantia nigra, locus coeruleus, and subcoeruleus ventralis of mesencephalic and pontine tegmentum. Close contacts between LPO fibres and catecholamine cells were visible in the nigra and the area ventralis tegmentalis. Retrograde labelling from LPO was found in the archistriatum, dorsomedial thalamic complex, nuclei lateralis anterior and superficialis parvicellularis thalami, substantia nigra, central gray, area ventralis tegmentalis of Tsai, and locus coeruleus and in cells dorsal to the decussation of brachium conjunctivum. Reciprocal connections were verified between the LPO and the following areas: dorsomedial thalamic complex, central gray, substantia nigra, area ventralis of Tsai, and locus coeruleus.
Assuntos
Gânglios da Base/fisiologia , Galinhas/anatomia & histologia , Vias Aferentes/citologia , Vias Aferentes/fisiologia , Animais , Gânglios da Base/citologia , Gânglios da Base/enzimologia , Encéfalo/anatomia & histologia , Encéfalo/citologia , Encéfalo/enzimologia , Vias Eferentes/citologia , Vias Eferentes/fisiologia , Feminino , Peroxidase do Rábano Silvestre , Imuno-Histoquímica , Sistema Límbico/citologia , Sistema Límbico/enzimologia , Sistema Límbico/fisiologia , Masculino , Vias Neurais/citologia , Vias Neurais/enzimologia , Vias Neurais/fisiologia , Fito-Hemaglutininas , Tirosina 3-Mono-Oxigenase/metabolismoRESUMO
The archistriatum of the domestic chick has been implicated in both fear behaviour and learning. However, relatively little is known about its organisation. The efferent connections of discrete anatomical regions of the chick archistriatum were therefore investigated by iontophoresis of the anterograde tracer Phaseolus vulgaris leucoagglutinin into its anterior, dorsal intermediate, ventral intermediate, medial, and posterior parts. The results of this study suggest that the chick archistriatum can be divided into two basic divisions according to whether they project to the following limbic structures: the hippocampal formation, septal areas, lobus parolfactorius, nucleus accumbens, ventral paleostriatum, and dorsomedial thalamus. The limbic archistriatum includes the posterior archistriatum and extends rostrally through the ventral intermediate archistriatum into the anterior archistriatum. The non-limbic archistriatum comprises the dorsal intermediate and medial archistriatum and largely gives rise to specific sensory, somatosensory, and motor telencephalofugal efferents. There may not be distinct borders between these two divisions of the chick archistriatum.
Assuntos
Encéfalo/fisiologia , Galinhas/anatomia & histologia , Vias Eferentes/anatomia & histologia , Sistema Límbico/anatomia & histologia , Telencéfalo/anatomia & histologia , Animais , Transporte Axonal , Vias Eferentes/fisiologia , Feminino , Sistema Límbico/fisiologia , Masculino , Fito-Hemaglutininas , Telencéfalo/fisiologiaRESUMO
In primary cultures of rat cerebellar granule cells the activation of excitatory amino acid receptors by 1-glutamate enhances the steady state level of c-fos proto-oncogene messenger RNA. This effect is blocked by magnesium (1mM) as well as by the glutamate receptor antagonist 2-amino-5-phosphono-valerate (APV). Among the other excitatory amino acid agonists N-methyl-D-Aspartate (NMDA) and quisqualate also increased c-fos mRNA content, the latter however to a significantly lesser extent, while kainate failed to modify the basal level of c-fos expression. The addition of the muscarinic agonist carbachol or of the inhibitory neurotransmitter GABA did not affect the basal level of c-fos mRNA. This data demonstrate for the first time that activation of signal transduction at a specific excitatory amino acid receptor subtype can increase the steady state level of c-fos proto-oncogene mRNA in primary culture of cerebellar neurons.
Assuntos
Regulação da Expressão Gênica , Proto-Oncogenes , Receptores de Neurotransmissores/metabolismo , Animais , Células Cultivadas , Cerebelo/citologia , RNA/análise , RNA/isolamento & purificação , RNA Mensageiro/análise , Ratos , Ratos Endogâmicos , Receptores de GlutamatoRESUMO
In this study we utilized electrophysiological and pathway tracing methods to investigate the projections from the medial prefrontal cortex to the midbrain raphe nuclei of the rat. Initial pathway tracing experiments using retrograde (horseradish peroxidase conjugates with wheatgerm agglutinin or choleratoxin B subunit) and anterograde (Phaseolus vulgaris-leucoagglutinin) markers demonstrated a direct, bilateral projection to the dorsal raphe nucleus and median raphe nucleus from the medial prefrontal cortex, and the origin of this projection was localized predominantly in the ventral medial prefrontal cortex (infralimbic/dorsal penduncular cortices). Using chloral hydrate-anaesthetized rats, extracellular recordings were made mostly from 5-hydroxytryptamine neurons in the dorsal raphe nucleus, but non-5-hydroxytryptamine dorsal raphe neurons were also studied, as was a small number of 5-hydroxytryptamine neurons in the median raphe nucleus. In an initial study, electrical stimulation of the ventral medial prefrontal cortex caused a post-stimulus inhibition in the majority (49/56) of dorsal raphe 5-hydroxytryptamine neurons tested (mean duration of inhibition, 200+/-17 ms); in some cases (8/56) the inhibition was preceded by short-latency (26 +/-3 ms) orthodromic activation, and a small number of cells was antidromically activated (6/56). Both single spiking and burst-firing 5-hydroxytryptamine neurons in the dorsal raphe nucleus responded in the same way, and median raphe 5-hydroxytryptamine neurons were also inhibited (5/5). In contrast, few (2/12) of the non-5-hydroxytryptamine dorsal raphe neurons tested were inhibited by ventral medial prefrontal cortex stimulation. The effects of stimulation of the dorsal and ventral medial prefrontal cortex were compared on the same raphe 5-hydroxytryptamine neurons (n=17): ventral medial prefrontal cortex stimulation inhibited 16/17 of these neurons while only 8/17 were inhibited by dorsal medial prefrontal cortex stimulation. Finally, the inhibitory effect of ventral medial prefrontal cortex stimulation on 5-hydroxytryptamine cell-firing was not altered by 5-hydroxytryptamine depletion with p-chlorophenylalanine or by systemic administration of the selective 5-hydroxytryptamine1A receptor antagonist WAY 100635. The latter findings indicate that the inhibition is not due to release of raphe 5-hydroxytryptamine which could theoretically arise from anti- or orthodromically activated 5-hydroxytryptamine neurons. Our results show that stimulation of the ventral medial prefrontal cortex causes a marked post-stimulus inhibition in the vast majority of midbrain raphe 5-hydroxytryptamine neurons tested. It seems likely that the projection from ventral medial prefrontal cortex to the midbrain raphe nuclei mediates the responses of 5-hydroxytryptamine neurons to cortical stimulation. These data are relevant to recent discoveries of functional and structural abnormalities in the medial prefrontal cortex of patients with major depressive illness.
Assuntos
Córtex Pré-Frontal/fisiologia , Núcleos da Rafe/fisiologia , Potenciais de Ação/fisiologia , Animais , Autorreceptores/antagonistas & inibidores , Estimulação Elétrica , Fenclonina/farmacologia , Masculino , Inibição Neural/fisiologia , Neurônios/metabolismo , Neurônios/fisiologia , Piperazinas/farmacologia , Piridinas/farmacologia , Núcleos da Rafe/citologia , Núcleos da Rafe/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores de Serotonina/efeitos dos fármacos , Receptores 5-HT1 de Serotonina , Serotonina/metabolismo , Antagonistas da Serotonina/farmacologiaRESUMO
Recent electrophysiological studies demonstrate that the ventral medial prefrontal cortex has a powerful inhibitory influence on 5-hydroxytryptamine (5-HT) neurones in the dorsal raphe nucleus. Here we utilised a combination of anatomical and electrophysiological methods to characterise the cellular substrate underlying this effect.Anterograde tracing (Phaseolus vulgaris leucoagglutinin) using electron microscopy demonstrated a pathway from the ventral medial prefrontal cortex that makes neuronal contacts throughout the dorsal raphe nucleus. These contacts were predominantly asymmetrical synapses adjoining GABA immunoreactive dendrites and spines. In vivo extracellular recordings were made in the dorsal raphe nucleus of the anaesthetised rat from a subpopulation of non-5-HT neurones. These neurones were fast-firing, irregular and with short spike width, properties strongly reminiscent of immunochemically identified GABA interneurones in other brain regions. Recordings of classical 5-HT neurones were also included. Electrical stimulation of the ventral medial prefrontal cortex elicited a rapid onset (16 ms latency), orthodromic excitation of the non-5-HT neurones (13/25 neurones). This stimulation also caused a pronounced inhibition of most 5-HT neurones tested, with a longer latency (30 ms), and this was partially blocked by locally applied bicuculline. These data provide the first evidence that the ventral medial prefrontal cortex influences the activity of large numbers of raphe 5-HT neurones by targeting a local network of GABA neurones. This circuitry predicts that physiological and pathological changes in the ventral medial prefrontal cortex will impact on significant parts of the forebrain 5-HT system.
Assuntos
Interneurônios/ultraestrutura , Mesencéfalo/ultraestrutura , Vias Neurais/ultraestrutura , Córtex Pré-Frontal/ultraestrutura , Núcleos da Rafe/ultraestrutura , Serotonina/metabolismo , Ácido gama-Aminobutírico/metabolismo , Potenciais de Ação/efeitos dos fármacos , Potenciais de Ação/fisiologia , Animais , Dendritos/efeitos dos fármacos , Dendritos/metabolismo , Dendritos/ultraestrutura , Estimulação Elétrica , Antagonistas GABAérgicos/farmacologia , Imuno-Histoquímica , Interneurônios/efeitos dos fármacos , Interneurônios/metabolismo , Masculino , Mesencéfalo/efeitos dos fármacos , Mesencéfalo/metabolismo , Microscopia Eletrônica , Sondas Moleculares , Inibição Neural/efeitos dos fármacos , Inibição Neural/fisiologia , Vias Neurais/fisiologia , Fito-Hemaglutininas , Córtex Pré-Frontal/fisiologia , Terminações Pré-Sinápticas/fisiologia , Terminações Pré-Sinápticas/ultraestrutura , Núcleos da Rafe/efeitos dos fármacos , Núcleos da Rafe/metabolismo , Ratos , Ratos Sprague-Dawley , Tempo de Reação/efeitos dos fármacos , Tempo de Reação/fisiologiaRESUMO
We have examined the effects of transient cerebral ischemia on performance of a one-trial passive avoidance task by chicks. Transient forebrain ischemia was induced by bilateral carotid artery occlusion for a period of 10 min. In one experimental group, ischemia was produced prior to training on the avoidance task whereas in the other group ischemic intervention was not made until 3 h after initial training. Sham-operated groups were matched to each of the experimental groups. All four groups were tested for retention of the avoidance response 24 h post-surgery. The sham-operated birds and those receiving post-training ischemia showed good retention of the avoidance response, whereas in birds which received ischemia prior to training there was significant amnesia. Neuronal damage, determined qualitatively using a silver impregnation method, was observed in several forebrain regions including the hippocampus, hyperstriatal regions, paleostriatum primitivum, ventral archistriatum, and lateral corticoid area. Damage was also observed in the Purkinje cells of the cerebellum. The behavioural and anatomical effects of transient forebrain ischemia have not been previously investigated in an avian species and the finding of significant amnesia for a learning task following ischemia is in good agreement with several behavioural studies in mammals.
Assuntos
Aprendizagem da Esquiva/fisiologia , Sistema Nervoso Central/fisiopatologia , Ataque Isquêmico Transitório/psicologia , Degeneração Neural/fisiologia , Animais , Sistema Nervoso Central/patologia , Galinhas , Ataque Isquêmico Transitório/patologia , NecroseRESUMO
Biliary obstruction of 14 and 28 days induced in the rat an increase of portal pressure (PP) and wedge hepatic vein pressure (WHVP); the higher these were, the longer was the obstruction. Occurrence of portal hypertension seemed related to portal and periportal fibrosis. Relief of obstruction after 14 days by bilioduodenal anastomosis brought back to normal PP and WHVP. In rats with longer obstruction periods, bilioduodenal anastomosis failed to lower PP and WHPV although biological signs of cholestasis returned to normal levels. These results suggest that portal hypertension may arise very shortly after biliary obstruction in rats and that it may persist in animals with a prolonged biliary obstruction despite an efficient bile drainage. In clinical conditions, such results would favor early treatment of lesions that usually cause prolonged bile duct obstruction, such as postoperative bile duct stenosis.
Assuntos
Doenças Biliares/complicações , Hipertensão Portal/etiologia , Animais , Pressão Sanguínea , Fígado/fisiopatologia , Cirrose Hepática Biliar/complicações , Masculino , Ratos , Fatores de TempoRESUMO
The ability of a heterotopic graft to prolong life in animals dying in hepatic coma due to liver necrosis has never been definitely established. Acute hepatic failure was produced in 15 dogs by an hour of total interruption of the hepatic blood supply. Nine dogs received an intrathoracic hepatic homograft concurrently. Nontransplanted dogs died within 21 hours in hepatic coma, while transplanted dogs survived significantly longer (P less than .001). In all transplanted dogs, biological signs of hepatic failure were corrected in 24 hours. In four animals, the graft was removed on the fifth postoperative day. Two of those survived for 10 and 15 days respectively with normal hepatic function. These results demonstrate that a temporary heterotopic liver transplant is able to support life during the acute, normally lethal phase in dogs with massive liver necrosis.
Assuntos
Transplante de Fígado , Doença Aguda , Animais , Cães , Fígado/irrigação sanguíneaRESUMO
The avian hippocampal formation (HP) is considered to be homologous to the mammalian hippocampus on the basis of topography, developmental origin and its role in processing spatial memory. However, the morphological organization of the avian HP is very different from that of mammals and components similar to the subdivisions of the mammalian structure are not readily recognizable. In passerine birds, three spatially and morphologically distinct populations of Calbindin immunoreactive neurones are found in the dorsolateral (DL), dorsomedial (DM) and ventral (V) aspects of HP. Iontophoresis of Phaseolus vulgaris leucoagglutinin revealed three consistently different projection patterns arising from the different subregions. Generally, there is a medial-to-lateral topographical organization of efferents in relation to the septal complex. The DL region could be paralleled to the subiculum of mammals with its main projections to the basal ganglia, the limbic archistriatum, the lateral septum and the paraxial meso-diencephalic centres. The 'V' subdivision is likely to be homologous to the Ammon's horn of mammals with its commissural projections to the contralateral HP. Based on its purely intrinsic connectivity, the DM region could be a good candidate for an equivalent of the dentate gyrus. Nitric oxide synthase (NOS) containing neural structures display a specific distribution within the hippocampal subregions which is uniform in all passerine species studied. However, there is a marked difference in the level of diffuse neuropil reactivity between food-storers versus non-storers. Unlike the mammalian homologue, avian hippocampal NOS positive neurones do not show a near complete co-localization with the inhibitory transmitter GABA.
Assuntos
Hipocampo/anatomia & histologia , Hipocampo/fisiologia , Memória/fisiologia , Aves Canoras/anatomia & histologia , Aves Canoras/fisiologia , Percepção Espacial/fisiologia , Vias Aferentes/anatomia & histologia , Vias Aferentes/fisiologia , Animais , Encéfalo/anatomia & histologia , Encéfalo/fisiologia , Vias Eferentes/anatomia & histologia , Vias Eferentes/fisiologia , Especificidade da EspécieRESUMO
The lobus parolfactorius (LPO) has been implicated in memory formation associated with passive avoidance training of young posthatch domestic chicks. The anatomical circuitry underlying memory formation in the chick is likely to involve the intermediate medial hyperstriatum ventrale-archistriatum-LPO arc. In the present work, we attempted to combine an ultrastructural characterisation of archistriatal afferent terminals in LPO with a description of the synaptic structure of LPO, in particular those elements that are immunoreactive to glutamate and GABA. Ventral archistriatal regions of 7-day-old domestic chicks were iontophoretically injected with Phaseolus vulgaris leucoagglutinin and the anterograde transport of the tracer was detected in the LPO. Selected samples from these birds, and also from other day-old chicks, were resin-embedded and reacted for L-glutamate or GABA, using the postembedding immunocytochemical method. Glutamate was abundant in the neuropil of LPO and typically seen in axodendritic or axospinous terminals with asymmetrical junctions, often multiple or perforated postsynaptic appositions. Conversely, GABA was often present in aspinous dendrites, probably representing GABAergic local circuit neurons or (putative striatonigral) projection neurons. Archistriatal efferents terminating in LPO formed small en passant or terminal varicosities, with infrequent asymmetrical axospinous synapses. Glutamate was not detected in these boutons. The findings imply that the functional state of LPO, based on powerful glutamatergic excitation, may be modified by a non-glutamatergic archistriatal input.