Role of ghrelin in the regulation of gastric acid secretion involving nitrergic mechanisms in rats.

Ghrelin, an endogenous ligand for growth hormone secretagogue receptor (GHS-R), has been identified in the rat and human gastrointestinal tract. Ghrelin has been proposed to play a role in gastric acid secretion. Nitric oxide (NO) was shown as a mediator in the mechanism of ghrelin action on gastric acid secretory function. However, there is a little knowledge about this topic. We have investigated the role of ghrelin in gastric acid secretion and the role of NO as a mediator. Wistar albino rats were used in this study. The pyloric sphincter was ligated through a small midline incision. By the time, saline (0.5 ml, iv) was injected to the control group, ghrelin (20 microg/kg, iv) was injected to the first experimental group, ghrelin (20 microg/kg, iv) + L-NAME (70 mg/kg, sc) was injected to the second group and L-NAME (70 mg/kg, sc) was administered to the third group. The rats were killed 3 h after pylorus ligation; gastric acid secretion, mucus content and plasma nitrite levels were measured. Exogenous ghrelin administration increased gastric acid output, mucus content and total plasma nitrite levels, while these effects of ghrelin were inhibited by applying L-NAME. We can conclude that ghrelin participates in the regulation of gastric acid secretion through NO as a mediator.

Possible impact of inflammatory status on C-telopeptide pyridinoline cross-links of type I collagen and osteocalcin levels around oral implants with peri-implantitis: a controlled clinical trial.

Detection of progression level of peri-implantitis may help in the prevention of oral implant failure. C-telopeptide pyridinoline crosslinks of Type I collagen (ICTP) and osteocalcin (OC) are specific markers of bone turnover and bone degradation. Determination of the ICTP and OC levels in the peri-implant sulcus fluid (PISF) may predict the metabolic and/or inflammatory changes in the peri-implant bone. The aim of this clinical study was to evaluate ICTP and OC levels in the PISF for oral implants with and without peri-implant bone destruction and correlate these levels with the traditional clinical peri-implant parameters (probing depth, plaque index, gingival index and gingival bleeding time index) and radiographic bone level measurements. Fifteen patients with 30 peri-implant sites with bone destruction (radiographic bone loss) and health were included. Clinical parameters were measured and PISF was collected from the sites. Peri-implant sulcus fluid ICTP and OC levels were detected by radioimmunoassay technique from PISF samples. All clinical parameters demonstrated a significant increase in peri-implantitis sites compared with healthy sites. The PISF volume of the peri-implantitis sites was also significantly higher than of the healthy peri-implant sites. Although not statistically significant, a trend of increase was demonstrated in ICTP PISF samples sampled from peri-implantitis sites compared with healthy sites. A significant increase was noticed for OC PISF level in peri-implantitis sites compared with healthy ones. As well as peri-implant clinical measurements, volumetric changes at PISF may be counted as an important clinical parameter to distinguish the bone destruction sites from healthy sites around oral implants.

The importance of data presentation regarding gingival crevicular fluid myeloperoxidase and elastase-like activity in periodontal disease and health status.

BACKGROUND: The enzymatic profile of gingival crevicular fluid (GCF) is being analyzed with increasing interest, but related studies lack a general consensus on most methodological points, including the appropriate mode of data presentation. METHODS: GCF myeloperoxidase (MPO) and elastase-like activity (ELA) levels were spectrophotometrically determined on a total of 60 subjects who were divided into three equal subgroups as early-onset periodontitis (EOP), adult periodontitis (AP), and healthy. GCF enzyme levels were calculated and evaluated both as total enzyme activity and enzyme concentration. The correlations between these GCF enzymes and clinical periodontal status were also analyzed. RESULTS: With both modes of data presentation, the results regarding MPO activity were consistent. When presented either as total MPO activity or MPO concentration, the periodontally healthy group showed significantly lower MPO activity than the two patient groups (P<0.05). However, two modes of data presentation did not match when GCF ELA was concerned. When data were reported as total ELA, the healthy group exhibited lower enzyme activity (0.02 +/- 0.001 U) than EOP (0.04 +/- 0.01 U) and AP (0.06 +/- 0.02 U) groups; but when reported as concentration, the highest ELA levels were seen in the healthy group (221 +/- 31.53 nmol/min/ml), followed by AP (98.63 +/- 23.03 nmol/min/ml) and EOP (70.49 +/- 12.02 nmol/min/ml) (P<0.05). A strong-positive and significant correlation existed between GCF MPO and ELA. Correlations with clinical parameters were mostly observed with total activities. CONCLUSIONS: The findings of the present study confirm the relationship between GCF ELA and MPO activity and periodontal disease and also support the functional relativity between the two enzymes. Furthermore, based on these findings, it can be suggested that data presentation by use of total activity seems to be more sensitive in both the reflection of the actual enzymatic profile of GCF and also the existing clinical periodontal status. For each GCF component, the validity of different modes of data presentation should be considered.

Hereditary gingival fibromatosis and sensorineural hearing loss in a 42-year-old man with Jones syndrome.

Hereditary gingival fibromatosis and sensorineural hearing loss in a 42-year-old man with Jones syndrome: Gingival fibromatosis is a rare disease, which can be seen as an isolated condition or associated with some uncommon syndromes. This case report describes the evaluation and treatment of a 42-year-old male patient with hereditary gingival fibromatosis, sensorineural hearing loss, undescended testis and maxillary odontogenic cyst (Jones Syndrome). Six years follow up of the index patient after the surgery revealed no recurrence of the gingival fibromatosis. This report also describes the anamnestic data of the patient's family that showed progressive deafness and gingival enlargement in three generations.

Two male patients with nevoid basal cell carcinoma syndrome from Turkey.

Nevoid basal cell carcinoma syndrome, also known as Gorlin's syndrome, is a familial autosomal dominant syndrome characterized by multiple basal cell carcinomas, multiple odontogenic keratocysts of the jaws, and skeletal anomalies. Both tumors and malformations of the central nervous system occur with nevoid basal cell carcinoma. Medulloblastoma is the primary brain tumor most frequently associated with this syndrome. The authors report in this article two male patients with nevoid basal cell carcinoma syndrome: a 22-year-old male patient with multiple odontogenic keratocysts, who had medulloblastoma at two years and multiple basal cell carcinoma at 10 years of age, and a 15-year-old male patient with skeletal abnormalities and multiple odontogenic keratocysts in the jaws.

Lymphangioma treatment with Nd-YAG laser.

In the oral cavity, lymphangioma is a rare, non-odontogenic, benign neoplasm which originates from lymph vessels. This lesion is common in the first decade of life and mostly occurs on the dorsal surface and lateral border of the tongue and rarely arises on the palate, gingiva, buccal mucosa and lips. Treatment of the lesion is surgical excision, however in recent years Neodymium Yitrium Aluminum Garnet (Nd-YAG) laser surgery has become favorable due to its many advantages. This case report presents the treatment of a buccal lymphangioma in a 10-year-old girl by Nd-YAG laser.

Cleidocranial dysplasia. Case report.

A 29-year-old Caucasian woman who presented with short stature and multiple unerupted supernumerary teeth is described. Radiological investigations of her cranial and skeletal abnormalities revealed cleidocranial dysplasia. Because of the advanced age of the patient and contraindication for orthodontic treatment, only surgical and prosthetic treatment were performed. The characteristics and treatments of this rare autosomal dominant disorder are discussed.

Hirsutism score and the severity of hyperandrogenism associated with polycystic ovary syndrome in the southeastern region of Turkey.

This study investigated the severity of hirsutism and its correlation with serum total testosterone (tT) levels in 87 patients with polycystic ovary syndrome (PCOS) and 85 healthy control subjects from the south-eastern region of Turkey. A wide range of variability exists in both hirsutism and modified Ferriman-Gallwey (mFG) scores. Similar mFG scores and serum tT levels were found compared with previous studies of general populations, but lower scores than those previously reported from Turkey. The incidence of hirsutism was lower in hyperandrogenic PCOS patients compared with non-hyperandrogenic PCOS patients. There was no correlation between serum tT levels and the rate of hirsutism in patients with PCOS. Heritability, testosterone receptor sensitivity, 5α reductase activity or environmental/socioeconomic factors may play a role in the development of clinical hirsutism. Larger scale studies are needed to clarify which other factors may be responsible and to confirm these results.

Early versus late loading of unsplinted TiUnite surface implants supporting mandibular overdentures: a 2-year report from a prospective study.

The aim of this study was to evaluate the clinical performance of the implants supporting mandibular overdentures, and to investigate the prosthodontic outcomes of the mandibular implant overdentures. Twenty edentulous patients participated in this study. Forty implants were placed in the canine areas of the mandibles of all patients using the 1-stage approach. New maxillary complete dentures and the mandibular implant overdentures were delivered to 10 patients in the test group 1 week after surgery, while new maxillary and mandibular complete prostheses were delivered to 10 patients in the control group. These conventional mandibular prostheses were converted to mandibular implant overdentures 3 months after surgery. No implants were lost neither in test nor in control group. The average ISQ values between the two groups were not statistically significant during 2 years (P > 0.05). The average marginal bone resorptions were 0.4 and 0.5 mm for the test and the control group after 2 years. The number of appointments required for the prosthodontic maintenance of the mandibular implant overdentures in the first year was higher than that in the second year, which was statistically significant(P < 0.001). The results of the study suggest that the 1-week early loading approach does not adversely influence the clinical performance of the implants supporting mandibular overdentures.

Bone density assessments of oral implant sites using computerized tomography.

The type and architecture of bone are considered to affect its load-bearing capacity and it has been indicated that poorer quality bone is associated with higher implant failure rates. To date, bone classifications have only provided subjective methods for pre-operative assessment, which can be considered unreliable. The aim of this study was to evaluate variations of the bone density in designated endosseous implant sites using computerized tomography. One hundred and thirty-one designated implant sites in 72 patients were utilized. Computerized tomography results indicated that bone densities may vary markedly when different areas of a designated implant site are compared. It has been observed that a difference in the bone density exists for the four regions within the oral area, with the anterior mandible yielding mean density values of 944.9+/-207 Hounsfield units (HU)>anterior maxilla, 715.8+/-190 HU>posterior mandible, 674.3+/-227 HU>posterior maxilla and 455.1+/-122 HU. Computerized tomography may be a useful tool for determining the bone density of interest areas before implant placement, and this valuable information about the bone quality provides dental practitioners to make better treatment planning regarding the implant positions.

Assessment of correlation between computerized tomography values of the bone, and maximum torque and resonance frequency values at dental implant placement.

The aim of this study was to determine the bone density in the designated implant sites using computerized tomography (CT), the fastening torque values of dental implants, and the implant stability values using resonance frequency analysis. Further aim was to evaluate a possible correlation between bone density, fastening torque and implant stability. Eighty-five patients were treated with 158 Brånemark System implants. CT machine was used for preoperative evaluation of the jawbone for each patient, and bone densities were recorded in Hounsfield units (HU). The fastening torque values of all implants were recorded with the OsseoCare equipment. Implant stability measurements were performed with the Osstell machine. The average bone density and fastening torque values were 751.4 +/- 256 HU and 39.7 +/- 7 Ncm for 158 implants. The average primary implant stability was 73.2 +/- 6 ISQ for seventy implants. Strong correlations were observed between the bone density, fastening torque and implant stability values of Brånemark System TiUnite MKIII implants at implant placement (P < 0.001). These results strengthen the hypothesis that it may be possible to predict and quantify initial implant stability and bone quality from pre-surgical CT diagnosis.

Cervicofacial actinomycosis (a case report).

Actinomycosis is now a very uncommonly diagnosed human disease. Major medical centers report approximately one case a year. The pathogenesis of actinomycosis is unclear, but trauma provides a portal of entry. The cervicofacial form is the most common and usually appears as a chronic swelling with one or more draining sinus tracts. For treatment, penicillin in high doses is suggested. This case report presents a case of cervicofacial actinomycosis in a 14 year old boy which was misdiagnosed for a long time and the treatment of the disease with mezlocillin, a new antibiotic in the treatment of actinomycosis.