RESUMO
Interconversion of estrogens by osteoblasts may play a role in regulating bone mass. As a first step toward exploring this possibility, we investigated the expression and activity of 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) in cultured human osteoblasts (HOB) and osteoblast-like osteosarcoma cells (MG63, TE85, and SaOS-2). Significant 17beta-HSD activity was detected in cell-free extracts of all bone cells with oxidation of estradiol to estrone predominating over reduction. Reverse transcription-polymerase chain reaction (RT-PCR) experiments showed that the mRNA for 17beta-HSD I was detectable only in MG63 cells, albeit at low levels, while 17beta-HSD II was present in MG63, TE85, and HOB, but not SaOS-2, and 17beta-HSD III was absent from each bone cell type. 17Beta-HSD IV was the only isoform present in all bone cells analyzed. Further analysis of the expression of 17beta-HSD IV in these bone cells by immunoblotting revealed both the full-length 83 kDa protein and the proteolytic 38 kDa form. The kinetic parameters for estradiol oxidation by purified recombinant 17beta-HSD IV (Km = 49.7 microM, Vmax = 79.4 nmol/minute/mg of protein) and its HSD-domain (Km = 79.4 microM, Vmax = 476 nmol/minute/mg of protein) were significantly higher than previously reported, but consistent with the values obtained with crude cell-free extracts of SaOS-2 cells (Km = 98.8 microM, Vmax = 0.07 nmol/minute/mg of protein) which contain only 17beta-HSD IV based on RT-PCR. These studies show that bone cells have the capacity to interconvert circulating estrogens and suggest that bone cell 17beta-HSDs serve primarily to attenuate the continuing actions of estradiol through conversion to its less potent form, estrone, under certain conditions.
Assuntos
17-Hidroxiesteroide Desidrogenases/metabolismo , Osso e Ossos/enzimologia , Enoil-CoA Hidratase , Isoenzimas/metabolismo , Complexos Multienzimáticos , 17-Hidroxiesteroide Desidrogenases/genética , Animais , Neoplasias Ósseas/enzimologia , Catálise , Células Cultivadas , Humanos , Hidroliases , Cinética , Osteossarcoma/enzimologia , Oxirredução , Proteína Multifuncional do Peroxissomo-2 , Reação em Cadeia da Polimerase , Proteínas Recombinantes/metabolismo , Spodoptera , Células Tumorais CultivadasRESUMO
Conditioned medium from cultures of rat medullary thyroid carcinoma CA-77 cells was used as a source for purification of the secreted forms of peptidyl alpha-amidating enzyme. The alpha-amidating enzyme activity was partially purified using a combination of weak anion exchange and gel filtration chromatography. Subsequent strong anion exchange chromatography at pH 6.0 resolved this partially pure enzyme into four distinct peaks of activity, termed Ia, Ib, II, and III. Peaks Ia and Ib exhibited broad pH optima between pH 6.0-8.5, whereas peaks II and III both exhibited pH optima at approximately pH 5.0. The peak III activity was further purified to electrophoretic homogeneity using hydrophobic interactive chromatography followed by strong anion exchange chromatography at pH 8.0. The enzyme exhibited an apparent molecular mass of 75K, a pH optimum of approximately pH 5.0, and a maximal turnover number of 580 min-1 in the presence of L-ascorbate. Kinetic studies demonstrated that the enzyme probably functions through a ping-pong mechanism with respect to the binding of the glycine-extended peptide substrate and the L-ascorbate cofactor. The peak III enzyme exhibits several distinctive characteristics compared to amidating enzymes isolated and characterized by other laboratories.
Assuntos
Isoenzimas/metabolismo , Oxigenases de Função Mista , Complexos Multienzimáticos , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/metabolismo , Neoplasias da Glândula Tireoide/enzimologia , Animais , Linhagem Celular , Cromatografia em Gel , Cromatografia por Troca Iônica , Meios de Cultura , Isoenzimas/isolamento & purificação , Cinética , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/isolamento & purificação , RatosRESUMO
A hepatic cytochrome P-450 isolated in an electrophoretically homogeneous form from phenobarbital-treated rats, exists predominantly in the low spin configuration (82% at 20 degrees C). The addition of saturating amounts of the substrate benzphetamine to this haemoprotein shifted the spin equilibrium to the high spin form, resulting in a doubling of the spin equilibrium constant from 0.220 to 0.539 at 20 degrees C. The histidine residues of this low spin, substrate-free cytochrome P-450 were modified in a time- and concentration-dependent manner with diethylpyrocarbonate, and progressive histidine modification resulted in a decrease of both the affinity and extent of substrate interaction with the haemoprotein. Although the histidine-modified haemoprotein maintained the capacity to undergo a temperature-dependent spin transition of the haem iron in the presence of saturating amounts of substrate, this capability was substantially decreased in comparison to the unmodified cytochrome. These results indicate that a histidine residue(s) is involved in the binding of substrate to cytochrome P-450 and hence interferes with the substrate-bound spin equilibrium. Our results further imply that histidine is probably not the sixth ligand of the substrate-free ferric form of the rat liver cytochrome P-450.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Histidina/metabolismo , Animais , Benzfetamina/metabolismo , Sítios de Ligação/efeitos dos fármacos , Fenômenos Químicos , Físico-Química , Dietil Pirocarbonato/farmacologia , Masculino , Microssomos Hepáticos/enzimologia , Ratos , Ratos Endogâmicos , Espectrofotometria Ultravioleta , TemperaturaRESUMO
The spectral interaction of a homologous series of alkyl-substituted benzenes and related compounds with purified mammalian cytochrome P-450 has been investigated. Each of the 10 hydrocarbons produced a Type I spectral change, indicative of a low to high spin transition of the haem iron of cytochrome P-450. The extent of perturbation of the cytochrome P-450 spin equilibrium varied for each compound and was used to quantify the spin shifts of the haemoprotein and consequently the substrate-bound spin equilibrium constant, K2. Molecular orbital calculations were utilised to determine the electronic structural parameters of the 10 hydrocarbons investigated, including the electrophilic and nucleophilic super-delocalizabilities summed over all atoms (sigma SE and sigma SN, respectively), the sum of the absolute values of net atomic charge (sigma QT) and the energy levels of both the highest occupied and lowest unoccupied molecular orbitals (E (HOMO) and E (LUMO) respectively). Multiple regression analyses were then utilized to generate quantitative structure-activity relationships between the above structural parameters and the substrate-bound spin equilibrium constant, K2. Good correlations were observed between sigma SE, sigma SN and sigma QT, indicating the importance of hydrophobicity and steric factors in the perturbation of the haemoprotein spin equilibrium. In addition, the electron-accepting potential of the hydrocarbons was an important structural feature and exhibited better correlations with K2 than the electron donating parameter. Taken collectively, our data show the importance of the hydrophobic and charge transfer characteristics of hydrocarbon substrates in dictating the position of the cytochrome P-450 spin equilibrium, and as such, provides a rational molecular explanation based on sound chemical principles for the differential interaction of hydrocarbons with cytochrome P-450.
Assuntos
Derivados de Benzeno/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Animais , Masculino , Matemática , Conformação Molecular , Ratos , Ratos Endogâmicos , Relação Estrutura-AtividadeRESUMO
The role of cytochrome b5 heme propionate groups in the functional interactions between cytochromes P-450 RLM5 and b5 has been investigated by comparing the capacity of RLM5 to interact with both native b5 and a b5 derivative in which the native heme was replaced with ferric protoporphyrin IX dimethyl ester (DME-b5). Both forms of b5 interacted with RLM5 causing an increase in the RLM5 spin state from 28 to 68% high-spin RLM5 at saturation, as judged using uv-visible spectrophotometry. However, DME-b5 exhibited a 7-fold weaker affinity for RLM5. The apparent dissociation constant (Kd) for the interaction between RLM5 and b5 was also shown to be a strong function of ionic strength, in a manner consistent with the involvement of electrostatic attraction in complex formation. Reconstitution of b5 into an RLM5-dependent monooxygenase system stimulated the p-nitroanisole demethylase rate about 25-fold and 7-ethoxycoumarin deethylase about 6-fold. DME-b5, however, produced only 30% of the stimulation of RLM5-dependent turnover of p-nitroanisole observed at equivalent concentrations of native b5 without a change in Km. With 7-ethoxycoumarin, turnover was 50% diminished. The diminished capacity of DME-b5 to stimulate RLM5-dependent substrate turnover was shown not to be due to impairment of electron flow between NADPH-cytochrome P-450 reductase and DME-b5, since the Km of reductase for DME-b5 is 2.5-fold lower, and the Vmax is actually increased, but rather to an impairment of some aspect of functional interaction between the DME-b5 and RLM5. The data show that complex formation between cytochrome P-450 and b5 involves electrostatic attraction mediated in part by cytochrome b5 heme propionate groups.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Grupo dos Citocromos b/metabolismo , Microssomos Hepáticos/enzimologia , Animais , Citocromos b5 , Eletroquímica , Esterificação , Heme/metabolismo , Masculino , Concentração Osmolar , Propionatos/metabolismo , Ligação Proteica , Protoporfirinas/metabolismo , Ratos , Ratos Endogâmicos , EspectrofotometriaRESUMO
Haem ligation in cytochrome P-450 has been reviewed and the nature of the fifth and sixth ligands of the haemoprotein in the ferric low-spin, ferric high-spin, ferrous and ferrous-carbon-monoxy states have been discussed. Factors controlling the cytochrome P-450 spin equilibrium have been described, including substrate and functional components of the mixed-function oxidase system. In addition, a thermodynamic model describing the interaction of substrate with ferric cytochrome P-450 has been developed in terms of the micro-equilibrium constants governing substrate binding. The functional significance of the cytochrome P-450 spin state with particular reference to control of the first electron reduction of the haemoprotein has been summarized, and a subsequent validation of the spin-redox coupling model of cytochrome P-450-dependent catalysis has been presented.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Heme/metabolismo , Ferro/metabolismo , Animais , Monóxido de Carbono/metabolismo , Catálise , Fenômenos Químicos , Físico-Química , Compostos Férricos/metabolismo , Compostos Ferrosos/metabolismo , Humanos , Cinética , Ligantes , NADP/metabolismo , Consumo de Oxigênio , Conformação ProteicaRESUMO
The interactions between purified rat hepatic microsomal cytochrome P-450 and the type I ligands benzphetamine and cytochrome b5 have been studied in the presence of phospholipid using difference spectrophotometry. Cytochrome b5 was shown to interact with cytochrome P-450 to form a tight 1:1 complex (Kd = 275 nM), in which the proportion of high spin cytochrome P-450 was increased from 7 to 30%. The presence of saturating cytochrome b5 was shown to cause a decrease in the apparent Kd for benzphetamine binding from 111 microM to 40 microM. Likewise, the presence of benzphetamine was shown to cause a decrease in the apparent dissociation constant for cytochrome b5 binding to cytochrome P-450 (Kd = 90 nM). The above interactions were resolved into the basic equilibria inter-relating the various ligation states of the hemoprotein in an energetically closed eight-state free energy coupling model and the relative magnitudes of the microequilibria were analyzed to determine the degree of coupling of the interactions between cytochrome P-450 and both benzphetamine and cytochrome b5. Consequently, the spin state changes in cytochrome P-450 induced by benzphetamine and cytochrome b5 binding were shown to arise because these ligands interact 7 and 4 times more tightly with high spin cytochrome P-450, respectively. Furthermore, the data revealed that these ligands interact at independent sites on cytochrome P-450. Thus the effects of cytochrome b5 upon benzphetamine binding and vice versa were rationalized simply in terms of an increase in the proportion of a high spin (high affinity) conformation of cytochrome P-450 brought about by pre-equilibration with the effector ligand, with the intrinsic binding affinities of the two ligands for the low or high spin states remaining relatively unaltered. The thermodynamic parameters associated with the interactions between cytochrome P-450 and cytochrome b5, determined from the temperature dependence of these interactions, revealed that these protein interactions are entropy driven and probably occur by a hydrophobic mechanism.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Grupo dos Citocromos b/metabolismo , Microssomos Hepáticos/metabolismo , Animais , Sistema Enzimático do Citocromo P-450/isolamento & purificação , Grupo dos Citocromos b/isolamento & purificação , Citocromos b5 , Cinética , Ligantes , Masculino , Matemática , Modelos Biológicos , Ligação Proteica , Ratos , Ratos Endogâmicos , Espectrofotometria , TermodinâmicaRESUMO
Selective methylamidation of NADPH-cytochrome P-450 reductase (EC 1.6.2.4) carboxyl groups was used to assess the relative importance of these groups in the enzyme-catalyzed reduction of cytochromes c, b5, and P-450. Methylamidation of as few as 7 mol of carboxyl groups per mol of reductase caused 80% inhibition of cytochrome c reduction, 50% inhibition of rat liver microsomal RLM3 reduction, and up to 90% inhibition in the capacity of the reductase to support reconstituted monooxygenase activities of RLM3, RLM5, and LM2. In marked contrast, cytochrome b5 reduction measured under comparable conditions was stimulated by 50%. The impaired interactions between the reductase and cytochromes P-450 LM2 and RLM5 were shown not to arise from an impaired capacity for the proteins to bind each other but more likely to be due to an inhibition of a step(s) subsequent to complex formation between the oxidized proteins. These results show that the reductase interacts functionally with cytochrome c and cytochromes P-450 on the one hand and cytochrome b5 on the other through different mechanisms.
Assuntos
NADPH-Ferri-Hemoproteína Redutase/metabolismo , Animais , Soluções Tampão , Fenômenos Químicos , Química , Cumarínicos/metabolismo , Sistema Enzimático do Citocromo P-450/metabolismo , Grupo dos Citocromos b/metabolismo , Grupo dos Citocromos c/metabolismo , Citocromos b5 , Transporte de Elétrons , Imidoésteres , Isoenzimas/metabolismo , Masculino , Metilaminas , Microssomos Hepáticos/enzimologia , Oxirredução , Coelhos , Ratos , Relação Estrutura-AtividadeRESUMO
A covalent complex between rabbit hepatic microsomal cytochromes P-450 isozyme 2 (LM2) and b5 was created and purified to greater than 95% homogeneity. The purified complex was largely comprised of the two cytochromes covalently attached at the interface of the functional electron transfer-effector complex as shown by the following evidence. The spin state of the LM2 within the complex was greater than the spin state of free LM2, and the addition of free cytochrome b5 (cyt b5) did not further increase the spin state of the LM2 within the complex. The spectral binding parameters (Kd and delta Amax) for the association of benzphetamine with LM2 in the complex were identical to those observed with free LM2 in the presence of saturating concentrations of free cyt b5 and much different from those observed for LM2 in the absence of cyt b5. Reconstituted monooxygenase activity of the covalent LM2-cyt b5 complex (LM2-cyt b5) in the presence of NADPH-cytochrome P-450 reductase was much higher than the activity of free LM2 and approached the activity of free LM2 in the presence of optimal concentrations of free cyt b5. Furthermore, the Km for the flavoprotein in supporting either free LM2 or LM2-cyt b5-dependent p-nitroanisole demethylation were similar. (iv) Less than 20-25% of the cyt b5 within the complex could be reduced by free NADH-cytochrome b5 reductase (NADH-cyt b5 reductase) albeit at a slow rate. The implications of this data to the current understanding of the mechanism and stoichiometry of protein interactions in the hepatic mixed function oxidase system are discussed.
Assuntos
Sistema Enzimático do Citocromo P-450/isolamento & purificação , Isoenzimas/isolamento & purificação , Microssomos Hepáticos/enzimologia , Animais , Sistema Enzimático do Citocromo P-450/metabolismo , Isoenzimas/metabolismo , Cinética , Substâncias Macromoleculares , CoelhosRESUMO
Native cytochrome b5 interacts with either RLM5 or LM2 to form tight equimolar complexes (Kd = 250 and 540 nM, respectively) in which the content of high spin cytochrome P-450 was substantially increased. Cytochrome b5 caused 3- and 7-fold increases in the binding affinities of RLM5 and LM2 for benzphetamine, respectively, and benzphetamine decreased the apparent Kd for cytochrome b5 binding. Upon formation of the ternary complex between cytochromes P-450, b5, and benzphetamine the percentage of cytochrome P-450 in the high spin state was increased from 28 to 74 (RLM5) and from 9 to 85 (LM2). Cytochrome b5 caused 13- and 7-fold increases in the rate of RLM5- and LM2-dependent p-nitroanisole demethylation, respectively. Amino-modified (ethyl acetimidate or acetic anhydride) cytochrome b5 produced results similar to those obtained above with native cytochrome b5. In contrast, modification of as few as 5 mol of carboxyl groups/mol of amidinated cytochrome b5 resulted in both a substantial loss of the spectrally observed interactions with either cytochrome P-450 LM2 or cytochrome P-450 RLM5, and in a loss of the cytochrome b5-mediated stimulation of p-nitroanisole demethylation catalyzed by either monooxygenase. In further studies, native and fully acetylated cytochromes b5 reoxidized carbonmonoxy ferrous LM2 at least 20 times faster than amidinated, carboxyl-modified cytochrome b5 derivatives. In contrast, amidination, or acetylation of amino groups, or amidination of amino groups plus methylamidination of the carboxyl groups did not appreciably slow the rate of reduction of the cytochrome b5 by NADPH-cytochrome P-450 reductase. Collectively, the results provide strong evidence for an essential role of cytochrome b5 carboxyl groups in functional interactions with RLM5 and LM2.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Grupo dos Citocromos b/metabolismo , Animais , Citocromos b5 , Masculino , Microssomos Hepáticos/enzimologia , Modelos Químicos , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Coelhos , EspectrofotometriaRESUMO
Water-soluble carbodiimide-catalyzed cross-linking of purified cytochrome P-450 LM2, cytochrome b5, and NADPH-cytochrome P-450 reductase was used to identify stable complexes formed between these proteins. High yields of P-450-b5 and P-450 reductase-b5 dimers, and lower yields of P-450 reductase-LM2 dimers were obtained. Substitution of native b5 and P-450 reductase with fully amidinated derivatives showed that LM2 and b5 were cross-linked exclusively through their respective amino and carboxyl groups. However, there appeared to be two complexation sites on the reductase which cross-link to b5 through amino groups and to LM2 through carboxyl groups respectively. A heterotrimer could not be identified following incubation of all three proteins together with EDC.
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Grupo dos Citocromos b/metabolismo , Microssomos Hepáticos/enzimologia , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Animais , Reagentes de Ligações Cruzadas , Citocromos b5 , Substâncias Macromoleculares , Microssomos Hepáticos/ultraestrutura , Fenobarbital/farmacologia , CoelhosRESUMO
Hypolipidaemic drugs induce peroxisomal proliferation in the liver and many induce the formation of the hepatic endoplasmic reticulum in general and the formation of cytochrome P-450 in particular. We have induced the formation of rat liver microsomal cytochrome P-450 by the administration of the hypolipidaemic drug clofibrate, isolated the endoplasmic reticulum, solubilized the cytochrome P-450 from these membranes and subdivided the cytochrome P-450 into four fractions by the use of hydrophobic, anionic, cationic and adsorption chromatography. One of these fractions (cytochrome P-450 fraction 1) was highly purified to a specific content of 17nmol of cytochrome P-450/mg of protein and the protein was active in a reconstituted enzyme system towards the 12- and 11-hydroxylation of the fatty acid, dodecanoic (lauric) acid, with preferential activity towards the 12-hydroxy metabolite. This reconstituted activity was absolutely dependent on NADPH, NADPH-cytochrome P-450 reductase and cytochrome P-450, indicating the role of the mixed-function oxidase system in the metabolism of lauric acid. Another fraction of the haemoprotein (cytochrome P-450 fraction 2) preferentially formed 11-hydroxylauric acid, whereas a third fraction (cytochrome P-450 fraction 3) exhibited only trace laurate oxidase activity and was similar to the phenobarbitone form of the haemoprotein in that these last two cytochromes rapidly turned-over the drug benzphetamine. The molecular weights and spectral properties of these cytochrome P-450 fractions are reported, along with the phenobarbitone-induced form of the enzyme and the nature of the cytochrome(s) induced by clofibrate pretreatment are discussed in the terms of possible haemoprotein heterogeneity.
Assuntos
Clofibrato/farmacologia , Sistema Enzimático do Citocromo P-450/metabolismo , Ácidos Láuricos/metabolismo , Fígado/enzimologia , Animais , Benzfetamina/metabolismo , Catálise , Sistema Enzimático do Citocromo P-450/isolamento & purificação , Eletroforese em Gel de Poliacrilamida , Retículo Endoplasmático/enzimologia , Indução Enzimática/efeitos dos fármacos , Cinética , Fígado/efeitos dos fármacos , Masculino , Oxigenases de Função Mista/metabolismo , Fenobarbital/farmacologia , Ratos , Ratos EndogâmicosRESUMO
A rapid, sensitive method for the quantification of glutaminyl cyclase activity has been developed. The assay involves enzymatic conversion of the model peptide Gln-Leu-Tyr-Glu-Asn-Lys-epsilon-(Dns)-OH to less than Glu-Leu-Tyr-Glu-Asn-Lys-epsilon-(Dns)-OH. Both the product and substrate of this reaction are detected in a single assay in quantities as low as 100 fmol using isocratic reverse phase high-performance liquid chromatography with fluorometric detection. The method is highly reproducible and ideally suited for the rapid analysis of large numbers of samples. The applications of the assay to both the detection of glutaminyl cyclase activity during enzyme purification and the more rigorous enzymology studies dependent on the precise measurement of initial reaction velocities are demonstrated.
Assuntos
Aciltransferases/análise , Aminoaciltransferases , Cromatografia Líquida de Alta Pressão/métodos , Animais , Bovinos , Compostos de Dansil/isolamento & purificação , Técnicas In Vitro , Oligopeptídeos/isolamento & purificação , Hipófise/metabolismo , Espectrometria de FluorescênciaRESUMO
Conversion of dansyl-Tyr-Val-Gly to dansyl-Tyr-Val-NH2 by recombinant type A rat 75-kDa peptidylglycine alpha-amidating enzyme (alpha-AE) is inactivated by ascorbate, dehydroascorbate, and hydrogen peroxide in a time- and concentration-dependent manner. Both ascorbate- and dehydroascorbate-mediated inactivation are saturable with apparent kinact/Kinact values of 1.7 and 0.23 s-1 M-1, respectively. Hydrogen peroxide-mediated inactivation is not saturable with a second-order rate constant of 50 s-1 M-1. Peptidyl-Gly substrates, EDTA, and H2O2 scavengers protect against ascorbate-mediated inactivation while EDTA and semidehydroascorbate scavengers protect against dehydroascorbate-mediated inactivation. Under similar conditions, ascorbate, dehydroascorbate, and H2O2 have no effect on the alpha-AE-catalyzed conversion of dansyl-Tyr-Val-alpha-hydroxyglycine to dansyl-Tyr-Val-NH2 which is consistent with the hypothesis that the 75-kDa enzyme consists of distinct peptidyl-Gly hydroxylase and peptidyl-alpha-hydroxyglycine lyase active sites.
Assuntos
Antioxidantes/farmacologia , Catecóis/farmacologia , Hidrolases/antagonistas & inibidores , Oxigenases de Função Mista/metabolismo , Complexos Multienzimáticos , Neoplasias da Glândula Tireoide/enzimologia , Sequência de Aminoácidos , Animais , Ácido Ascórbico/farmacologia , Linhagem Celular , Ácido Desidroascórbico/farmacologia , Peróxido de Hidrogênio/farmacologia , Hidrolases/isolamento & purificação , Cinética , Camundongos , Oxigenases de Função Mista/genética , Oxigenases de Função Mista/isolamento & purificação , Dados de Sequência Molecular , Oligopeptídeos , Ratos , Proteínas Recombinantes/antagonistas & inibidores , Proteínas Recombinantes/isolamento & purificação , Proteínas Recombinantes/metabolismo , TransfecçãoRESUMO
The capacity of four native P-450 enzymes to interact with cytochrome b5 was compared and discussed in terms of spin shift and metabolism. Two-dimensional electrophoresis was employed as a tool to aid in characterization of the different enzymes isolated from liver microsomes of the untreated rat. RLM5 had a pl of 7.4 and RLM3 had a pl of 7.1. Two new forms isolated by this laboratory, RLM2 and RLM5a, differed from other forms of cytochrome P-450 characterized to date. The pl values of these forms were 7.35 and 7.6, respectively. The interaction of all four enzymes with cytochrome b5 differed. Cytochrome b5 caused a major low to high spin transition when added to RLM5. The latter hemoprotein was 28% high spin at 25 degrees C and was shifted to 55% high spin by cytochrome b5. RLM5a shifted from 4% high spin to 15% high spin under comparable conditions. In contrast, RLM2 and RLM3 were both minimally influenced by cytochrome b5, reaching only 8% high spin. Cytochrome b5 did not appreciably influence the rates of metabolism of aminopyrine, benzphetamine, testosterone, or p-nitroanisole with RLM2 or RLM3. However, with RLM5 and RLM5a, rates of aminopyrine and benzphetamine demethylation and testosterone hydroxylation were increased to about 130% with RLM5 and up to 200% with RLM5a. The demethylation of p-nitroanisole was stimulated by cytochrome b5, 3.5-fold with RLM5 and 14-fold with RLM5a. In no case was the ratio of monohydroxy metabolites of testosterone altered by the addition of cytochrome b5, indicating an effect on Vmax rather than Km.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Grupo dos Citocromos b/metabolismo , Animais , Catálise , Citocromos b5 , Eletroforese em Gel de Poliacrilamida , Técnicas In Vitro , Focalização Isoelétrica , Masculino , Microssomos Hepáticos/enzimologia , Peso Molecular , Ratos , EspectrofotometriaRESUMO
The interaction between NADPH-cytochrome P-450 reductase and a series of cytochrome P-450 isozymes was investigated using UV-visible spectrophotometry. In the absence of substrate the interactions between the reductase and RLM3, RLM5, and RLM5a were tight, exhibiting sub-micromolar dissociation constants and resulted in type I spectra of varying magnitude from which the following increases in the proportion of high spin hemoprotein were calculated; RLM3 (7%), RLM5 (36%), RLM5a (6%), LM2 (29%), RLM2 (0%). Preincubation of LM2 with its type I substrate benzphetamine increased the affinity of the cytochrome for the reductase. Using initial estimates of the P-450 spin states in the absence of reductase in conjunction with the spectral binding data and equations relating these parameters to the microequilibria for the association of reductase with high or low spin P-450, RLM3, RLM5, RLM5a and LM2 were shown to bind significantly more tightly to high spin P-450. The relevance of this data to the understanding of spin state influence on P-450 reduction is discussed.
Assuntos
Sistema Enzimático do Citocromo P-450 , NADPH-Ferri-Hemoproteína Redutase , Animais , Sistema Enzimático do Citocromo P-450/metabolismo , Cinética , Microssomos Hepáticos/enzimologia , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Ligação Proteica , Conformação Proteica , Coelhos , Ratos , Análise Espectral , Especificidade por SubstratoRESUMO
Interrogation of the public expressed sequence tag (EST) data base with the sequence of preproaprotinin identified ESTs encoding two potential new members of the Kunitz family of serine protease inhibitors. Through reiterative interrogation, an EST contig was obtained, the consensus sequence from which encoded both of the novel Kunitz domains in a single open reading frame. This consensus sequence was used to direct the isolation of a full-length cDNA clone from a placental library. The resulting cDNA sequence predicted a 252-residue protein containing a putative NH2-terminal signal peptide followed sequentially by each of the two Kunitz domains within a 170-residue ectodomain, a putative transmembrane domain, and a 31-residue hydrophilic COOH terminus. The gene for this putative novel protein was mapped by use of a radiation hybrid panel to chromosome 19q13, and Northern analysis showed that the corresponding mRNA was expressed at high levels in human placenta and pancreas and at lower levels in brain, lung, and kidney. An endogenous soluble form of this protein, which was designated as placental bikunin, was highly purified from human placenta by sequential kallikrein-Sepharose affinity, gel filtration, and C18 reverse-phase chromatography. The natural protein exhibited the same NH2 terminus as predicted from the cloned cDNA and inhibited trypsin, plasma kallikrein, and plasmin with IC50 values in the nanomolar range.
Assuntos
Glicoproteínas/biossíntese , Glicoproteínas/química , Glicoproteínas de Membrana , Placenta/metabolismo , Inibidores de Serina Proteinase/biossíntese , Inibidor da Tripsina de Soja de Kunitz , Inibidores da Tripsina/química , Sequência de Aminoácidos , Sequência de Bases , Cromatografia de Afinidade , Cromatografia em Gel , Sequência Conservada , Feminino , Glicoproteínas/isolamento & purificação , Humanos , Dados de Sequência Molecular , Fases de Leitura Aberta , Gravidez , Homologia de Sequência de Aminoácidos , Inibidores de Serina Proteinase/química , Inibidores de Serina Proteinase/isolamento & purificaçãoRESUMO
In the present studies, a novel form of highly purified cytochrome P-450 (cytochrome P-452) isolated from the hepatic microsomes of clofibrate-pretreated rats has been compared to the major isozymes isolated from the hepatic microsomes of rats pretreated with phenobarbital (cytochrome P-450) and 2-naphthoflavone (cytochrome P-447) using a number of biochemical criteria. The results show that these three isozymes exhibit marked structural differences from each other as judged by a complete lack of immunochemical cross-reactivity between the isozymes and the heterologous rabbit serum antibodies using Ouchterlony double diffusion, and non-identity between the limited proteolytic digestion maps of the three isozymes obtained in the presence of chymotrypsin, papain and Staphylococcus aureus V8 proteases. Furthermore, the three isozymes exhibited clear differences in their monomeric molecular weights determined on calibrated sodium dodecyl sulphate/polyacrylamide gel electrophoresis in gels of varying acrylamide concentration. Substantial differences were also observed in the substrate specificities of the isozymes, which were reflected in differences in the turnover rates and positional selectivities of the hemoproteins for some model substrates. In addition, the isozymes differed in their substrate binding affinities and their ability to interact with purified hepatic microsomal cytochrome b5, as judged using difference spectrophotometry. Finally, subtle differences were detected in the ultraviolet visible absorbance spectra of the hemoproteins in the ferric, ferrous, and carbonmonoxyferrous states. Taken collectively, the above data provides compelling evidence that fundamental differences exist between these cytochrome P-450 isozymes, further establishing the uniqueness of the major form of cytochrome P-450 induced by clofibrate pretreatment.
Assuntos
Clofibrato/farmacologia , Sistema Enzimático do Citocromo P-450/isolamento & purificação , Isoenzimas/isolamento & purificação , Microssomos Hepáticos/enzimologia , Animais , Sistema Enzimático do Citocromo P-450/biossíntese , Indução Enzimática/efeitos dos fármacos , Isoenzimas/biossíntese , Masculino , Peso Molecular , Fragmentos de Peptídeos/análise , Ratos , Ratos Endogâmicos , Espectrofotometria , Especificidade por SubstratoRESUMO
Results are presented that support our hypothesis [Backes, W. L., Sligar, S. G., & Schenkman, J. B. (1980) Biochem. Biophys. Res. Commun. 97, 860-867] that the multiphasic reduction kinetics of cytochrome P-450 are, in part, due to the spin equilibrium of the ferric hemoprotein. The disappearance of the high-spin charge-transfer band at 650 nm during reduction of the hemoprotein by NADPH was fast, exhibiting a rate constant greater than that of the fast phase of reduction measured by formation of the carbon monoxide adduct. In contrast, the disappearance of the ferric low-spin form of the cytochrome was at a considerably slower rate. A mathematical expression of the fractional content of high-spin cytochrome P-450 was obtained by comparing the ratio of the initial rate of change in the fraction of total oxidized cytochrome remaining to the initial rate of change in the fraction of high-spin ferric P-450 remaining. Results supporting the model were obtained by using both microsomes and purified cytochrome P-450 RLM5. The calculation from experimental data yielded results that were similar to those obtained by different extrapolation methods used for estimation of the amount of high-spin cytochrome P-450, supporting further the proposed relationship between the spin equilibrium and the reduction kinetics of this hemoprotein.