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1.
BMC Pulm Med ; 23(1): 207, 2023 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-37316870

RESUMO

BACKGROUND: For patients with advanced non-small-cell lung cancer (NSCLC) with EGFR mutations, the suggested course of action is epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs). Even with a high disease control rate, a majority of patients develop acquired EGFR-TKIs resistance and eventually advance. To increase the benefits of treatment, clinical trials are increasingly exploring the value of EGFR-TKIs combined with angiogenesis inhibitors as a first-line treatment in advanced NSCLC carrying EGFR mutations. METHOD: Using PubMed, EMBASE and Cochrane Library, to locate published full-text articles in print or online, a thorough literature search was done from the database's inception to February 2021. Additionally, oral presentation RCTs from ESMO and ASCO were obtained. We sifted out RCTs that used EGFR-TKIs along with angiogenesis inhibitors as first-line therapy for advanced EGFR-mutant NSCLC. ORR, AEs, OS, and PFS were the endpoints. Review Manager version 5.4.1 was used for data analysis. RESULTS: One thousand eight hundred twenty-one patients were involved in 9 RCTs. According to the results, combining EGFR-TKIs with angiogenesis inhibitors therapy prolonged PFS of advanced EGFR-mutation NSCLC patients on the whole [HR:0.65 (95%CI: 0.59~0.73, P<0.00001)]. No significant statistical difference was identified between the combination group and single drug group in OS(P=0.20) and ORR (P=0.11). There are more adverse effects when EGFR-TKIs are used in combination with angiogenesis inhibitors than when used alone. CONCLUSION: The combination of EGFR-TKIs and angiogenesis inhibitors prolonged PFS in patients with EGFR-mutant advanced NSCLC, but the OS and ORR benefit was not significant, and the risk of adverse events was higher, more pronounced with hypertension and proteinuria; PFS in subgroups suggested that the combination was associated with better PFS in the smoking, liver metastasis, and no brain metastasis groups, and the included studies suggested that the smoking group , liver metastasis group, and brain metastasis group may have a potential OS benefit.


Assuntos
Carcinoma Pulmonar de Células não Pequenas , Neoplasias Hepáticas , Neoplasias Pulmonares , Humanos , Inibidores da Angiogênese/efeitos adversos , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/genética , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/genética , Receptores ErbB/genética
2.
BMC Pediatr ; 22(1): 465, 2022 08 03.
Artigo em Inglês | MEDLINE | ID: mdl-35918695

RESUMO

BACKGROUND: Milk fat globule membrane (MFGM), natural to breast milk, is essential for neonatal development, but lacking from standard infant formulas. OBJECTIVES: To evaluate the safety and tolerability of MFGM supplementation in formula for infants 0 to 12 months. METHODS: In a prospective, multicentre, double-blind, randomized trial, healthy term infants were randomized to a standard formula (SF, n = 104) or an MFGM-enriched formula (MF, n = 108) for 6 months and a corresponding follow-on formula until 12 months. Exclusively breast-fed infants (n = 206) were recruited as the reference group (BFR). Tolerance and safety events were recorded continuously. Anthropometric measurements were assessed at enrolment, 42 days and 4, 6, 8 and 12 months. RESULTS: Infants (n = 375) completed the study with average dropout of < 20%. Stool frequency, color, and consistency between SF and MF were not significantly different throughout, except the incidence of loose stools in MF at 6 months being lower than for SF (odds ratio 0.216, P < 0.05) and the frequency of green-colored stools at 12 months being higher in MF (CI 95%, odds ratio 8.92, P < 0.05). The BFR had a higher frequency of golden stools and lower rate of green stools (4-6 months) than the two formula-fed groups (P < 0.05). SF displayed more diarrhoea (4.8%) than MF (1%) and BFR (1%) at the 8-month visit (P < 0.05). BFR (0-1%) had significantly less (P < 0.05) lower respiratory infections than MF (4.6-6.5%) and SF (2.9-5.8%) at 6- and 8-months, respectively. Formula intake, frequency of spit-up/vomiting or poor sleep were similar between SF and MF. Growth rate (g/day) was similar at 4, 6, 8 and 12 months between the 3 groups, but growth rate for BFR was significantly higher than for SF and MF at 42 days (95% CI, P = 0.001). CONCLUSIONS: MFGM-enriched formula was safe and well-tolerated in healthy term infants between 0 and 12 months, and total incidences of adverse events were similar to that for the SF group. A few differences in formula tolerance were observed, however these differences were not in any way related to poor growth.


Assuntos
Glicolipídeos , Fórmulas Infantis , Aleitamento Materno , China , Método Duplo-Cego , Feminino , Glicoproteínas , Humanos , Lactente , Recém-Nascido , Gotículas Lipídicas , Leite Humano , Estudos Prospectivos
3.
Int J Mol Sci ; 23(17)2022 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-36077287

RESUMO

N6-methyladenosine (m6A) is one of the most abundant internal modifications of mRNA, which plays important roles in gene expression regulation, and plant growth and development. Vir-like m6A methyltransferase associated (VIRMA) serves as a scaffold for bridging the catalytic core components of the m6A methyltransferase complex. The role of VIRMA in regulating leaf development and its related mechanisms have not been reported. Here, we identified and characterized two upland cotton (Gossypium hirsutum) VIRMA genes, named as GhVIR-A and GhVIR-D, which share 98.5% identity with each other. GhVIR-A and GhVIR-D were ubiquitously expressed in different tissues and relatively higher expressed in leaves and main stem apexes (MSA). Knocking down the expression of GhVIR genes by the virus-induced gene silencing (VIGS) system influences leaf cell size, cell shape, and total cell numbers, thereby determining cotton leaf morphogenesis. The dot-blot assay and colorimetric experiment showed the ratio of m6A to A in mRNA is lower in leaves of GhVIR-VIGS plants compared with control plants. Messenger RNA (mRNA) high-throughput sequencing (RNA-seq) and a qRT-PCR experiment showed that GhVIRs regulate leaf development through influencing expression of some transcription factor genes, tubulin genes, and chloroplast genes including photosystem, carbon fixation, and ribosome assembly. Chloroplast structure, chlorophyll content, and photosynthetic efficiency were changed and unsuitable for leaf growth and development in GhVIR-VIGS plants compared with control plants. Taken together, our results demonstrate GhVIRs function in cotton leaf development by chloroplast dependent and independent pathways.


Assuntos
Regulação da Expressão Gênica de Plantas , Gossypium , Adenosina/análogos & derivados , Cloroplastos/metabolismo , Gossypium/genética , Gossypium/crescimento & desenvolvimento , Gossypium/metabolismo , Metilação , Metiltransferases/metabolismo , Folhas de Planta/crescimento & desenvolvimento , Folhas de Planta/metabolismo , RNA Mensageiro/metabolismo , RNA de Plantas/metabolismo
4.
Zhen Ci Yan Jiu ; 47(6): 553-8, 2022 Jun 25.
Artigo em Chinês | MEDLINE | ID: mdl-35764525

RESUMO

Acupuncture has an unique advantage in treatment of ischemic stroke, which not only promotes the repair of synaptic structure and function, but also regulates the transmission of neurotransmitters and receptors, as well as induces glial cell to repair neurons, and then to protect them. At present, the mechanism study on acupuncture for advancing synaptic plasticity in cerebral ischemia is of the high priority. In the paper, from the following three aspects, i.e. synaptic plasticity (structure and function), interaction between synapses (neurotransmitters and receptors), and interconnection between synapses and environments (synaptic-glial structure), the progress of mechanism study of acupuncture in recent years was reviewed on regulating synaptic plasticity in treatment of ischemic stroke.


Assuntos
Terapia por Acupuntura , Isquemia Encefálica , AVC Isquêmico , Isquemia Encefálica/terapia , Humanos , Plasticidade Neuronal/fisiologia , Sinapses/fisiologia
5.
Front Cell Dev Biol ; 9: 634347, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33777942

RESUMO

Cerebral ischemia is one of the leading causes of death. Reperfusion is a critical stage after thrombolysis or thrombectomy, accompanied by oxidative stress, excitotoxicity, neuroinflammation, and defects in synapse structure. The process is closely related to the dephosphorylation of actin-binding proteins (e.g., cofilin-1) by specific phosphatases. Although studies of the molecular mechanisms of the actin cytoskeleton have been ongoing for decades, limited studies have directly investigated reperfusion-induced reorganization of actin-binding protein, and little is known about the gene expression of actin-binding proteins. The exact mechanism is still uncertain. The motor cortex is very important to save nerve function; therefore, we chose the penumbra to study the relationship between cerebral ischemia-reperfusion and actin-binding protein. After transient middle cerebral artery occlusion (MCAO) and reperfusion, we confirmed reperfusion and motor function deficit by cerebral blood flow and gait analysis. PCR was used to screen the high expression mRNAs in penumbra of the motor cortex. The high expression of cofilin in this region was confirmed by immunohistochemistry (IHC) and Western blot (WB). The change in cofilin-1 expression appears at the same time as gait imbalance, especially maximum variation and left front swing. It is suggested that cofilin-1 may partially affect motor cortex function. This result provides a potential mechanism for understanding cerebral ischemia-reperfusion.

6.
RSC Adv ; 10(35): 20852-20861, 2020 May 27.
Artigo em Inglês | MEDLINE | ID: mdl-35517738

RESUMO

According to its thermodynamic equilibrium analysis and strong exothermic characteristics, the major challenge of syngas methanation is to develop a high-efficient low-temperature catalyst with superior sintering resistance. In this study, bimetal-based SBA-15 catalysts were prepared via a citric acid-assisted impregnation method and applied in CO methanation. The obtained catalysts were characterized via X-ray diffraction, N2 adsorption-desorption, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, H2 temperature-programmed reduction and other techniques. Combining the structural characterization of the fresh and used catalyst, the function of the organic additive and metal promoters was revealed. The catalysts exhibited superior low-temperature activity and excellent sintering resistance owing to the electron migration from the additive metal to Ni, strong interaction between the metal and support and the confinement effect of the support. The catalyst with Mo as a promotor exhibited the best dispersion and the largest surface concentration of nickel, which resulted in its highest catalytic activity among the catalysts. The design and preparation of a highly effective catalyst can provide novel insight into the preparation of other catalysts.

7.
RSC Adv ; 10(72): 44397, 2020 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-35532403

RESUMO

[This corrects the article DOI: 10.1039/C6RA27334C.].

8.
Plant Physiol Biochem ; 143: 83-93, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31491703

RESUMO

Ethylene insensitive 3 (EIN3), a key transcription factor in ethylene signal transduction, play important roles in plant stress signaling pathways. In this study, we isolated and characterized an EIN3-like gene from cotton (Gossypium hirsutum), designated as GhEIN3. GhEIN3 is highly expressed in vegetative tissues, and its expression is induced by 1-aminocyclopropane-1-carboxylic acid (ACC) and NaCl. Ectopic expression of GhEIN3 in Arabidopsis elevated plants' response to ethylene, which exhibit smaller leaves, more root hairs, shorter roots and hypocotyls. The germination rate, survival rate and root length of GhEIN3 transgenic plants were significantly improved compared to wild type under salt stress. GhEIN3 transgenic plants accumulated less H2O2 and malondialdehyde (MDA), while higher superoxide dismutase (SOD) and peroxidase (POD) activities were detected under salt stress. In addition, expression of several genes related to reactive oxygen species (ROS) pathway and ABA signaling pathway was increased in the GhEIN3 transgenic plants under salt stress. In contrast, virus-induced gene silencing (VIGS) of GhEIN3 in cotton enhanced the sensitivity of transgenic plants to salt stress, accumulating higher H2O2 and MDA and lower SOD and POD activities compared to control plants. Collectively, our results revealed that GhEIN3 might be involved in the regulation of plant response to salt stress by regulating ABA and ROS pathway during plant growth and development.


Assuntos
Gossypium/efeitos dos fármacos , Gossypium/metabolismo , Proteínas de Plantas/metabolismo , Proteínas de Arabidopsis/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/genética , Gossypium/genética , Malondialdeído/metabolismo , Proteínas de Plantas/genética , Plantas Geneticamente Modificadas/efeitos dos fármacos , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Tolerância ao Sal/genética , Plantas Tolerantes a Sal/efeitos dos fármacos , Plantas Tolerantes a Sal/genética , Plantas Tolerantes a Sal/metabolismo , Cloreto de Sódio/farmacologia
9.
Sci Rep ; 7(1): 2788, 2017 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-28584307

RESUMO

Plant JAZ (Jasmonate ZIM-domain) proteins play versatile roles in multiple aspects of plant development and defense. However, little is known about the JAZ family in allotetraploid upland cotton (Gossypium hirsutum) so far. In this study, 30 non-redundant JAZ genes were identified in upland cotton through genome-wide screening. Phylogenetic analysis revealed that the 30 proteins in cotton JAZ family are further divided into five groups (I - V), and members in the same group share highly conserved motif structures. Subcellular localization assay demonstrated that GhJAZ proteins are localized in the cell nucleus. Quantitative RT-PCR analysis indicated that GhJAZs display different expression patterns in cotton tissues, and most of them could be induced by Jasmonic (JA). Furthermore, some GhJAZ genes are preferentially expressed in cotton ovules and fibers, and showed differential expression in ovules of wild type cotton and fiberless mutant (fl) during fiber initiation. GhJAZ proteins could interact with each other to form homodimer or heterodimer, and they also interacted with some JA signaling regulators and the proteins involved in cotton fiber initiation. Collectively, our data suggested that some GhJAZ proteins may play important roles in cotton fiber initiation and development by regulating JA signaling as well as some fiber-related proteins.


Assuntos
Estudo de Associação Genômica Ampla , Gossypium/genética , Família Multigênica , Proteínas de Plantas/genética , Proteínas Repressoras/genética , Sequência de Aminoácidos , Sequência Conservada , Gossypium/metabolismo , Espaço Intracelular/metabolismo , Mutação , Fenótipo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Ligação Proteica , Transporte Proteico , Proteínas Repressoras/química , Proteínas Repressoras/metabolismo
10.
Sci Rep ; 7(1): 10118, 2017 08 31.
Artigo em Inglês | MEDLINE | ID: mdl-28860559

RESUMO

TCP proteins are plant-specific transcription factors (TFs), and perform a variety of physiological functions in plant growth and development. In this study, 74 non-redundant TCP genes were identified in upland cotton (Gossypium hirsutum L.) genome. Cotton TCP family can be classified into two classes (class I and class II) that can be further divided into 11 types (groups) based on their motif composition. Quantitative RT-PCR analysis indicated that GhTCPs display different expression patterns in cotton tissues. The majority of these genes are preferentially or specifically expressed in cotton leaves, while some GhTCP genes are highly expressed in initiating fibers and/or elongating fibers of cotton. Yeast two-hybrid results indicated that GhTCPs can interact with each other to form homodimers or heterodimers. In addition, GhTCP14a and GhTCP22 can interact with some transcription factors which are involved in fiber development. These results lay solid foundation for further study on the functions of TCP genes during cotton fiber development.


Assuntos
Gossypium/genética , Proteínas de Plantas/genética , Fatores de Transcrição/genética , Regulação da Expressão Gênica de Plantas , Genoma de Planta , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Fatores de Transcrição/química , Fatores de Transcrição/metabolismo
11.
Nanoscale Res Lett ; 9(1): 291, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24959109

RESUMO

The major component in green tea polyphenols, epigallocatechin-3-gallate (EGCG), has been demonstrated to prevent carcinogenesis. To improve the effectiveness of EGCG, liposomes were used as a carrier in this study. Reverse-phase evaporation method besides response surface methodology is a simple, rapid, and beneficial approach for liposome preparation and optimization. The optimal preparation conditions were as follows: phosphatidylcholine-to-cholesterol ratio of 4.00, EGCG concentration of 4.88 mg/mL, Tween 80 concentration of 1.08 mg/mL, and rotary evaporation temperature of 34.51°C. Under these conditions, the experimental encapsulation efficiency and size of EGCG nanoliposomes were 85.79% ± 1.65% and 180 nm ± 4 nm, which were close with the predicted value. The malondialdehyde value and the release test in vitro indicated that the prepared EGCG nanoliposomes were stable and suitable for more widespread application. Furthermore, compared with free EGCG, encapsulation of EGCG enhanced its inhibitory effect on tumor cell viability at higher concentrations.

12.
Artigo em Chinês | WPRIM | ID: wpr-271627

RESUMO

<p><b>OBJECTIVE</b>To introduce 8 patients with isolated congenital anosmia and to discuss the clinical manifestations, imaging characteristics and family characteristics of this rarely seen disorder.</p><p><b>METHODS</b>Eight patients with isolated congenital anosmia treated between April 2007 and April 2012 were reviewed retrospectively. There were 4 males and 4 females. A detailed medical history collection, physical examination, nasal endoscopy, T&T and Sniffin'Sticks subjective olfactory function tests, olfactory event-related potentials sinonasal computed tomography scan and sex hormones level monitoring were performed in all patients. Seven cases underwent magnetic resonance image of olfactory pathway examination.</p><p><b>RESULTS</b>All patients were anosmia without evidence of other defects. ENT physical examination, nasal endoscopy and computed tomography scan were normal except 4 cases with obvious nasal septum deviation, 2 cases with concha bullosa. Subjective olfactory test indicated all of them were anosmia. Olfactory event-related potentials were obtained in only 1 patient. Magnetic resonance imaging revealed the smaller or atrophy olfactory bulb and olfactory tract in five cases, the absence of olfactory bulbs and tracts in two case. A female patient did not have MRI examination because of wearing IUDs. Detection of 8 patients of sex hormones were normal. Family characteristics: 3 patients showed family inheritance pattern.</p><p><b>CONCLUSIONS</b>The diagnosis of isolated congenital anosmia should be based on chief complaint, medical history, physical examination, olfactory test, nasal endoscopy, olfactory testing, olfactory imaging and olfactory event-related potentials. Magnetic resonance image of olfactory pathway and olfactory event-related potentials have important value for the diagnosis. More attention should be paid to the genetic susceptibility of the family.</p>


Assuntos
Adolescente , Adulto , Idoso , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Imageamento por Ressonância Magnética , Transtornos do Olfato , Diagnóstico , Genética , Condutos Olfatórios , Estudos Retrospectivos
13.
Artigo em Chinês | WPRIM | ID: wpr-245927

RESUMO

<p><b>OBJECTIVE</b>To explore the experience and value of overlay tympanoplasty.</p><p><b>METHODS</b>Sixty-three ears with overlay tympanoplasty were reviewed and followed up for the external auditory canal, tympanic membrane and hearing.</p><p><b>RESULTS</b>The diseases of the patients included middle ear cholesteatoma in 25 ears and chronic suppurative otitis media in 38 ears. The surgical techniques involved three kinds: overlay tympanoplasty, overlay tympanoplasty with canal wall up mastoidectomy and overlay tympanoplasty with canal wall down mastoidectomy. In middle ear cholesteatoma and suppurative otitis media patients, the case received the three techniques are 4, 17, 4 ears and 19, 18, 1 ears respectively. All patients gained stage I incision cure. Followed up for 0.5 to 3.5 years respectively, the external auditory canal was wide and tympanic membrane gained a good shape. The hearing in all case kept intact or increased while hearing decrease did not occur. Complications were free in patients with punctual visit.</p><p><b>CONCLUSIONS</b>Overlay tympanoplasty has positive significance in treating the chronic otitis media with the merits of standard procedure, sufficient operative field and thorough erosion elimination.</p>


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adulto Jovem , Colesteatoma da Orelha Média , Cirurgia Geral , Otite Média Supurativa , Cirurgia Geral , Resultado do Tratamento , Timpanoplastia , Métodos
14.
Artigo em Chinês | WPRIM | ID: wpr-317300

RESUMO

<p><b>OBJECTIVE</b>To constitute the animal model of unilateral olfactory nerve transection and observe the expression level and distribution of odorant receptors.</p><p><b>METHODS</b>Thirty-two rats were divided into two groups: the olfactory nerve transection group (20) and the control group (12). The former group received the operation to transect the left olfactory nerve following the left olfactory bulb was exposed under microscope and the latter group did not give any disposal. At every stage of five days, two weeks, four weeks and six weeks after the operation, five rats from the nerve transection group and three from the control group were anaesthetized simultaneously, and olfactory epithelium were taken out after transcardial perfusion, then paraffin imbedding. Coronal sections were sliced for HE staining to observe the thickness changes of the olfactory epithelium, and for in situ hybridization (ISHs) to investigate the expression of olfactory receptor genes (Olr287, Olr226, Olr1493 and Olr1654) in the epithelium, also to evaluate the changes of the expression level and location of the selected receptors during the regeneration of olfactory epithelium.</p><p><b>RESULTS</b>HE staining showed that 5 days after the operation cell quantity and thickness of the olfactory epithelium decreased obviously, which increased gradually 2 or 4 weeks after operation. After 6 weeks' recovery, the thickness of the epithelium could reach the control level. The pattern of cell staining by ISH showed a specific spatial distribution along the anteroposterior (AP) and dorsoventral (DV) axis. Evidence suggested that odorant receptors were distributed in continuous and multiple overlapping bands in the normal or nerve transected-recovered epithelium rather than in the conventionally accepted three or four zones. The data also demonstrated that the distribution of sensory neuron types, as identified and defined by odorant receptor expression, was restored to normal or nearly so by 6 weeks after operation. Likewise, the numbers of probe-labeled neurons in the nerve transected-recovered had an obvious decrease 5 days after olfactory nerve transection. Reactive cells (x(-) +/- s) of Olr1493 in the operated side was (53.9 +/- 19.9), compared with (419.0 +/- 21.2) in the unoperated side, there was statistic significance between them (t = 63.960, P < 0.01). Reactive cells increased gradually according to the regeneration of the epithelium, and were nearly equivalent to the normal side 6 weeks later without significant differentiation (t = 2.600, P > 0.05), according to the absolute positive cells in the operated and unoperated side of (417.8 +/- 32.4) and (445.3 +/- 10.0) respectively.</p><p><b>CONCLUSION</b>The regeneration of the sensory neurons and receptors, both the number and the distribution, can recover to normal after olfactory nerve transection.</p>


Assuntos
Animais , Masculino , Ratos , Mucosa Olfatória , Metabolismo , Nervo Olfatório , Metabolismo , Cirurgia Geral , Traumatismos do Nervo Olfatório , Neurônios Receptores Olfatórios , Biologia Celular , Metabolismo , Ratos Sprague-Dawley , Receptores Odorantes , Genética , Metabolismo
15.
Artigo em Chinês | WPRIM | ID: wpr-309437

RESUMO

<p><b>OBJECTIVE</b>To observe the effects of Betamethasone on the cyclic adenosine monophosphate (cAMP) in olfactory receptor neurons (ORNs) and explore the possible mechanisms of the recovery of olfactory disorders by steroid treatment</p><p><b>METHODS</b>ORNs membrane was extracted and incubated with 0.1 mg/ml and 1.0 mg/ml Betamethasone. The concentrations of cAMP were measured by enzyme-linked immunosorbent assay at different times.</p><p><b>RESULTS</b>Compared with the control group, all Betamethasone groups showed differences, indicating 0.1 mg/ml (P < 0.05) and 1.0 mg/ml (P < 0.01) Betamethasone could rise the concentration of cAMP. The Betamethasone had obvious effects on cAMP production in rat ORNs and there was a dose-dependent effect. There was no difference between 5 minutes groups and 30 minutes groups.</p><p><b>CONCLUSIONS</b>Steroid hormone could enhance the production of cAMP of ORNs. Steroid hormone may thus contribute to the recovery of olfactory disorders partially, at least, through the effect on AC-cAMP in olfactory transduction.</p>


Assuntos
Animais , Ratos , Betametasona , Farmacologia , AMP Cíclico , Metabolismo , Potenciais da Membrana , Neurônios Receptores Olfatórios , Metabolismo , Técnicas de Patch-Clamp , Ratos Wistar , Transdução de Sinais
16.
Artigo em Chinês | WPRIM | ID: wpr-262814

RESUMO

<p><b>OBJECTIVE</b>To investigate the clinical features, diagnosis and treatment of primary ciliary dyskinesia (PCD).</p><p><b>METHODS</b>Three cases of PCD received endoscopic sinus surgery and were followed up for life quality and recovery. Among these 3 cases, two were twin brothers and the other girl was twin born with a healthy brother. The mucosa of inferior turbinate was extracted prior to the operation without narcotic and decongestant. The ultrastructure of mucosal cilia was detected with electron microscope. Nine exons of gene DNAH5 and chromosome in one case and her fraternal twin were evaluated.</p><p><b>RESULTS</b>Nasal and sinus CT imaging of the 3 cases showed chronic pansinusitis (1 case accompanied with situs inversus according with the diagnosis of Kartagener syndrome). The nasal polyp was resected, and the sinuses were opened. The twin brothers received the adenoidectomy. All patients felt nasal ventilation improved while the surgical field still covered with thick discharges during follow-up for 2 - 4 years. Ciliary ultrastructures of the three cases showed lateral dynein absent, the sequence of 9 exons of DNAH5 and chromosome presented no change in the fraternal twins.</p><p><b>CONCLUSIONS</b>Surgery could improve the symptoms of sinusitis in PCD. Change of ciliary ultrastructure was an important indication of its pathological changes and molecular biology evaluation needs further study.</p>


Assuntos
Criança , Feminino , Humanos , Masculino , Adulto Jovem , Dineínas do Axonema , Metabolismo , Cílios , Éxons , Síndrome de Kartagener , Diagnóstico , Genética , Patologia , Sinusite , Diagnóstico , Genética
17.
Artigo em Chinês | WPRIM | ID: wpr-325287

RESUMO

<p><b>OBJECTIVE</b>To analyze the impact of olfactory nerve transection on the apoptosis of mice olfactory receptor neurons (ORN), and discuss the reliability of this experimental model.</p><p><b>METHODS</b>After olfactory nerve transection of mice, anterograde horseradish peroxidase (HRP) tracing was performed to confirm the completion of nerve transection. On 8 h, 2 d, 3 d and 5 d after surgery, TdT mediated deoxyuridine triphosphate-biotin nick end labelling (TUNEL) was used to observe the apoptosis of ORN, while relative semi-quantitative RT-PCR and immunohistochemistry were used to reflect the expression of olfactory marker protein (OMP, special marker of mature ORN) in olfactory epithelium.</p><p><b>RESULTS</b>No HRP label was observed in olfactory bulb after olfactory nerve transaction. Both TUNEL-positive and OMP-positive cells were ORN. After the surgery, TUNEL-positive cells increased remarkably and peaked on 2 d after the surgery. Meanwhile OMP mRNA in olfactory epithelium began to decrease markedly till 5 d after the surgery, and the olfactory epithelium got thinner accordingly.</p><p><b>CONCLUSIONS</b>This experimental model can be used reliably to sever mice olfactory nerve and manipulate simultaneous apoptosis of mice ORN.</p>


Assuntos
Animais , Camundongos , Apoptose , Denervação , Camundongos Endogâmicos C57BL , Nervo Olfatório , Biologia Celular , Metabolismo , Cirurgia Geral , Neurônios Receptores Olfatórios , Metabolismo , Patologia
18.
Artigo em Chinês | WPRIM | ID: wpr-679447

RESUMO

Objective To study the characteristics of dynamic susceptibility-contrast(DSC)MR perfusion curves,color images and perfusion values in pre-operative brain metastasis.Methods Twenty- eight brain metastases underwent DSC MR perfusion imaging by using a first-pass T_2~* echo-planar sequence. The patients' data were transferred to on-line workstation.Time-signal intensity curves,color perfusion maps and rCBV,rMTT values in both tumor parenchyma and peri-tumor edema were analyzed,and independent t- test was used and P0.05).Conclusion Different originated brain metastases have nearly same characteristics in DSC MR perfusion imaging.

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