RESUMO
The growth of omic data presents evolving challenges in data manipulation, analysis and integration. Addressing these challenges, Bioconductor provides an extensive community-driven biological data analysis platform. Meanwhile, tidy R programming offers a revolutionary data organization and manipulation standard. Here we present the tidyomics software ecosystem, bridging Bioconductor to the tidy R paradigm. This ecosystem aims to streamline omic analysis, ease learning and encourage cross-disciplinary collaborations. We demonstrate the effectiveness of tidyomics by analyzing 7.5 million peripheral blood mononuclear cells from the Human Cell Atlas, spanning six data frameworks and ten analysis tools.
RESUMO
The growth of omic data presents evolving challenges in data manipulation, analysis, and integration. Addressing these challenges, Bioconductor1 provides an extensive community-driven biological data analysis platform. Meanwhile, tidy R programming2 offers a revolutionary standard for data organisation and manipulation. Here, we present the tidyomics software ecosystem, bridging Bioconductor to the tidy R paradigm. This ecosystem aims to streamline omic analysis, ease learning, and encourage cross-disciplinary collaborations. We demonstrate the effectiveness of tidyomics by analysing 7.5 million peripheral blood mononuclear cells from the Human Cell Atlas3, spanning six data frameworks and ten analysis tools.
RESUMO
We present 16S amplicon data derived from the nest materials of three species of Australian stingless bees (Meliponini). This data set reveals the diversity of bacteria associated with these materials. It will serve as a valuable baseline for further study of the nest microbiome and comparison with the stingless bee microbiota.
RESUMO
Strength auditing of European honey bee (Apis mellifera Linnaeus, 1758 [Hymenoptera: Apidae]) colonies is critical for apiarists to manage colony health and meet pollination contracts conditions. Colony strength assessments used during pollination servicing in Australia typically use a frame-top cluster-count (Number of Frames) inspection. Sensing technology has potential to improve auditing processes, and commercial temperature sensors are widely available. We evaluate the use and placement of temperature sensing technology in colony strength assessment and identify key parameters linking temperature to colony strength. Custom-built temperature sensors measured hive temperature across the top of hive brood boxes. A linear mixed-effect model including harmonic sine and cosine curves representing diurnal temperature fluctuations in hives was used to compare Number of Frames with temperature sensor data. There was a significant effect of presence of bees on hive temperature and range: hives without bees recorded a 5.5°C lower mean temperature and greater temperature ranges than hives containing live bees. Hives without bees reach peak temperature earlier than hives with bees, regardless of colony strength. Sensor placement across the width of the hive was identified as an important factor when linking sensor data with colony strength. Data from sensors nearest to the hive geometric center were found to be more closely linked to colony strength. Furthermore, a one unit increase in Number of Frames was significantly associated with a mean temperature increase of 0.36°C. This demonstrates that statistical models that account for diurnal temperature patterns could be used to predict colony strength from temperature sensor data.