Diatom and macroinvertebrate communities dynamic: A co-occurrence pattern analysis on plastic substrates.

Wetlands are habitats that provide numerous ecosystem services, but are often understudied and threatened by anthropogenic pollution, particularly plastic pollution. Macroplastics are a significant component of plastic litter that have high biological impacts but are often understudied. Previous studies have highlighted negative impacts on biota, but there is a lack of information about the communities of micro and macro organisms that settle on macroplastic litter. In this context, we investigated the colonization patterns and community structures of diatoms and macroinvertebrates on virgin substrates composed of two different plastic polymers, polystyrene and polyethylene terephthalate, located at two different depths in a protected wetland in Central Italy over a period of 10 months. The results show that diatom community is not highly structured by competitive forces and aggregation patterns emerges. In contrast, macroinvertebrate community appears to be randomly structured, without the presence of patterns following specific assembly rules. Randomness in macroinvertebrates assemblages could highlight the presence of different niches available for settlement of different taxa. Combined matrix analyses show that diatoms and macroinvertebrates co-occur, and their community assemblages are sometimes structured, while they appeared to be randomly assembled at other times. Whenever non-randomness of diatoms and macroinvertebrates co-occurrences was detected, it suggested aggregation. Moreover, the possible predatory relationship between different macroinvertebrates taxa should be investigated, as it could reveal important scenarios in the establishment of macroinvertebrate structured communities on plastic litter, including taxa that exploit different ecological niches. This could lead to an enrichment of the biological community within areas impacted by plastics.

Passive biomonitoring of airborne microplastics using lichens: A comparison between urban, natural and protected environments.

Currently, natural and urban ecosystems are affected by different types of atmospheric deposition, which can compromise the balance of the environment. Plastic pollution represents one of the major threats for biota, including lichens. Epiphytic lichens have value as bioindicators of environmental pollution, climate change, and anthropic impacts. In this study, we aim to investigate the lichen bioaccumulation of airborne microplastics along an anthropogenic pollution gradient. We sampled lichens from the Genera Cladonia and Xanthoria to highlight the effectiveness of lichens as tools for passive biomonitoring of microplastics. We chose three sites, a "natural site" in Altipiani di Arcinazzo, a "protected site" in Castelporziano Presidential estate and an "urban site" in the centre of Rome. Overall, we sampled 90 lichens, observed for external plastic entrapment, melt in oxygen peroxide and analysed for plastic entrapment. To validate the method, we calculated recovery rates of microplastics in lichen. Particularly, 253 MPs particles were detected across the 90 lichen samples: 97 % were fibers, and 3 % were fragments. A gradient in the number of microplastic fibers across the sites emerged, with increasing accumulation of microplastics from the natural site (n = 58) to the urban site (n = 116), with a direct relationship between the length and abundance of airborne microplastic fibers. Moreover, we detected the first evidences of airborne mesoplastics entrapped by lichens. On average, the natural site experienced the shortest fibre length and the centre of Rome the longest. No differences in microplastics accumulation emerged from the two genera. Our results indicated that lichens can effectively be used for passive biomonitoring of microplastic deposition. In this scenario, the role of lichens in entrapping microplastics and protecting pristine areas must be investigated. Furthermore, considering the impact that airborne microplastics can have on human health and the effectiveness of lichens as airborne microplastic bioindicators, their use is encouraged.

Epiplastic microhabitats for epibenthic organisms: a new inland water frontier for diatoms.

Plastic pollution is widespread in each type of ecosystems. However, the colonization events of microorganisms on plastics seem to be neglected in inland waters. Therefore, in this study we analyze the possible colonization on the surface (hereafter epiplastic microhabitats) of two typology of plastic supports by diatom community. Specifically, we located 20 supports in expanded polystyrene and 20 in polyethylene terephthalate both floating and dipped (~ 1 m) in a central Italian shallow water pond, in order to evaluate the diachronic colonization of diatoms from November 2019 to August 2020. Our result showed the tendency in colonizing both epiplastic microhabitats without significant differences in number of species; additionally, depth does not appear to affect the number of species. As regard the temporal colonization, the number of species tends to increase over time from autumn-winter to spring-summer in both types of epiplastic microhabitats and depth. Instead, increase in dominance of some species over time has been demonstrated: only a few species keep a high number of individuals compared to the others; therefore, the number of individuals within the species is not uniformly distributed. These results suggest the tendency of diatom community to colonize plastic supports in lentic waters, and this evidence can be very important because artificial supports can increase the surface available for the settlement of the algae community with an increase of productivity and the colonization of new communities of different taxa. Further studies are mandatory to investigate the possible effects on the epiplastic community and the ecological implications in freshwater environments.

New ecological frontiers in the plastisphere: Diatoms and macroinvertebrates turnover assessment by a traits-based approach.

To date, there are very few studies regarding the colonization of artificial substrates in wetlands by macroinvertebrates and diatoms and even fewer are the studies in Italy that take into consideration the diatomic guilds and the biological and ecological traits proposed in literature. Wetlands are at the forefront through the most delicate and threatened freshwater ecosystems. In this study, we want to evaluate the colonization capacity of plastics of diatoms and macroinvertebrates and characterize the diatomic and macroinvertebrate communities using a "traits-based" approach focusing on the colonization of virgin substrates made of polystyrene and polyethylene terephthalate. The study was conducted within the 'Torre Flavia wetland Special Protection Area' a protected wetland area in Central Italy. The study was conducted from November 2019 to August 2020. The results obtained in this study show a tendency of diatom species to colonize artificial plastic supports placed in lentic environments without differences related to the plastic type and water depth. There is also a greater number of species belonging to the "Motile" guild, endowed with a high motility that they exploit to search for more ecologically suitable habitats for settlement. Macroinvertebrates, prefer settlement on polystyrene supports, those on the surface, probably due to the anoxic conditions present on the bottom and the physical structure of the polystyrene that provides shelter to many animal taxa. The analysis on traits highlighted the establishment of an ecologically diverse community mainly formed by univoltine organisms, with dimensions between 5 and 20 mm, predators, choppers and scrapers feeding on plant organisms and animals, but without the formation of a clear ecological system, that is, without evidence of ecological relationships established between two or more taxa. Our research can contribute to underline the ecological complexity of biota inhabiting plastic litter in freshwaters and the implications for plastic-impacted ecosystems biodiversity enrichment.

Mutual Avoidance in the Spectacled Salamander and Centipede: A Discrepancy between Exploratory Field and Laboratory Data.

Interactions between amphibians and arthropods encompass a wide range of ecological relationships, predominantly characterized by predator-prey dynamics, with adult amphibians as the predators. In some instances, the roles are reversed. This study focuses on the potential predator-prey relationship between the spectacled salamander (Salamandrina perspicillata) and the centipede Scolopendra cingulata in Central Italy. Building upon previous research on chemical cue perception in amphibians, we investigated potential olfactory cue-mediated avoidance behaviours exhibited by S. perspicillata towards the potential predator S. cingulata through field observations and manipulative experiments. In a natural site, we estimated the degree of negative co-occurrence between the study species under shelters and found an avoidance pattern between S. perspicillata and S. cingulata in refuges. However, when the study species were forced to choose between sharing or not sharing a given shelter, through a manipulative experiment, the avoidance pattern was not confirmed. Potential determinants contributing to the avoidance pattern observed in nature are discussed. Our exploratory results represent a good example of how what often appears to be a strong observation-based pattern in natural settings needs to be carefully scrutinized. Hypotheses testing through experiments in controlled environments remains a valuable approach to exclude potentially misleading processes.

Teratogenic effects of environmental concentration of plastic particles on freshwater organisms.

Given the widespread presence of plastics, especially in micro- and nanoscale sizes, in freshwater systems, it is crucial to identify a suitable model organism for assessing the potential toxic and teratogenic effects of exposure to plastic particles. Until now, the early life stage of freshwater organisms and the regeneration capacity in relation to plastic particles exposure is a still poorly investigated topic. In this study, we examine the teratogenic effect on diatom Cocconeis placentula and cnidarian Hydra vulgaris under controlled exposure conditions of poly(styrene-co-methyl methacrylate) (P(S-co-MMA)) particles. Significant effects were observed at the lowest concentrations (0.1 µg/L). A significant increase in the teratological frequency in C. placentula and a significant decrease in the regeneration rate in H. vulgaris were found at the lowest concentration. The delay in hydra regeneration impaired the feeding capacity and tentacles reactivity at 96 h of exposure. No effects on diatom growth were observed upon exposure to P(S-co-MMA) particles (0.1, 1, 100, 10,000 µg/L) for 28 days and these findings agree with other studies investigating algal growth. The application of the Teratogenic Risk Index, modified for diatoms, highlighted a moderate risk for the lowest concentration evaluating C. placentula and low risk at the lowest and the highest concentrations considering H. vulgaris. This study suggests the importance of testing organisms belonging to different trophic levels as diverse teratogenic effects can be found and the need to evaluate environmentally relevant concentrations of plastic particles.

Is the weight of plastic litter correlated with vegetal wrack? A case study from a Central Italian beach.

This study analyzes the occurrence and distribution of plastic litter and the entrapment of plastic by wrack beached on a natural reserve. Large microplastics (2.5 - 5 mm) were the most abundant plastic size category detected. The main color and shape were white and fragment, respectively. The plastics entrapped by egagropiles were mainly transparent fibers. We analyzed the correlation between the weights of plastic litter and vegetal wrack in two transects, selected for their different environmental characteristics. The transect closer to a breakwater showed a significant positive correlation between the weights of plastics and wrack, while the other transect suggested a casual pattern of plastic deposition on the beach. Further research is suggested to focus on the role of breakwaters in altering marine currents and enhancing plastic beaching.