RESUMO
When Avibacterium paragallinarum reference strain 0083 (serovar A) was grown in an iron-restricted culture medium, the expression of the 60, 68 and 93 kDa outer membrane proteins increased as compared with normal media. Sera of chickens experimentally infected with Av. paragallinarum recognized these iron-restriction induced proteins, suggesting their expression in vivo. The three outer membrane proteins were identified as transferrin receptor and iron transport proteins by mass spectroscopy and a search in sequence databases. As these proteins have been reported to be regulated by the Fur protein in many bacteria, we investigated, through molecular methods, the presence of the fur gene in Av. paragallinarum. A candidate fur gene of Av. paragallinarum was amplified by polymerase chain reaction using complementary primers to conserved regions of fur gene sequences from members of the Pasteurellaceae family. The nucleotide sequence of the cloned gene, from ATG to TAA stop codon, was 453 base pairs in length and the deduced amino acid sequence showed 94% identity with Fur sequences of Actinobacillus pleuropneumoniae and Haemophilus ducreyi. The Av. paragallinarum deduced Fur protein (17.8 kDa) amino acid sequence contains the N-terminal helix-turn-helix DNA-binding domain and the two iron-binding sites in the C-terminal end, typical of other described Fur proteins. The study of iron-restriction-induced proteins and the mechanism regulating their expression could lead to an understanding of the responses of Av. paragallinarum to survive in an iron-restricted environment on host mucosal surfaces.
Assuntos
Proteínas de Bactérias/genética , Pasteurellaceae/genética , Receptores da Transferrina/metabolismo , Proteínas Repressoras/genética , Sequência de Aminoácidos , Proteínas de Bactérias/metabolismo , Sequência de Bases , Western Blotting , Clonagem Molecular , Biologia Computacional , Primers do DNA/genética , Eletroforese em Gel de Poliacrilamida , Ferro/metabolismo , Espectrometria de Massas , Dados de Sequência Molecular , Receptores da Transferrina/genética , Proteínas Repressoras/metabolismo , Análise de Sequência de DNA , Homologia de SequênciaRESUMO
It was found that 48 hour cultures of Actinobacillus pleuropneumoniae secreted proteases into the medium. Electrophoresis in polyacrylamide gels (10%) copolymerized with porcine gelatin (0.1%), of the 70% (NH4)2SO4 precipitate from the culture supernatants, displayed protease activities of different molecular weights: > 200, 200, 90, 80, 70 and 50 kDa. They had activity over a broad range of pHs (4-8), with an optimal pH of 6-7. All were inhibited by 10 mM EDTA, and reactivated by 10 mM calcium. They were stable at -20 degrees C for more than a month. The proteases also degraded porcine IgA and porcine, human, and bovine hemoglobin, although they appeared to be less active against the hemoglobins. The IgA was totally cleaved in 48 h, using supernatants concentrated with polyvinyl pyrrolidone or the 70% (NH4)2SO4. Extracellular proteases could play a role in virulence.
Assuntos
Actinobacillus pleuropneumoniae/enzimologia , Endopeptidases/metabolismo , Gelatina/metabolismo , Hemoglobinas/metabolismo , Imunoglobulina A/metabolismo , Infecções por Actinobacillus/metabolismo , Infecções por Actinobacillus/veterinária , Animais , Colostro/imunologia , Meios de Cultura , Humanos , Hidrólise , Imunoglobulina A Secretora/metabolismo , Abscesso Pulmonar/microbiologia , Abscesso Pulmonar/veterinária , Inibidores de Proteases/farmacologia , Reprodutibilidade dos Testes , Especificidade por Substrato , Suínos , Doenças dos Suínos/metabolismoRESUMO
A high molecular-mass proteolytic enzyme of Actinobacillus pleuropneumoniae serotype 1, was purified from culture supernatants (CSN) by using DEAE-cellulose and sepharose-4B-gelatin chromatography. In 10% SDS-polyacrylamide gels copolymerized with porcine gelatin, the protease showed a single band of activity of > 200 kDa. However, minor molecular-mass proteolytic bands were observed when the protease was electrophoresed in the presence of either 5% beta-mercaptoethanol, 50 mM dithiothreitol, or 0.25 M urea. Furthermore, when the > 200-kDa purified protein was passed through a sucrose gradient, several bands with proteolytic activity were found: 62, 90, 190, and 540 kDa. The proteolytic activity was increased in the presence of calcium or zinc and was not affected after being heated at 90 degrees C for 5 min. Proteolytic activities were also observed in CSN from all A. pleuropneumoniae serotypes and biotypes. The purified protease hydrolyzed porcine IgA and IgG in vitro. In addition, by immunoblot the protease was recognized by serum of naturally infected pigs with serotypes 1 and 5, and by serum of pigs experimentally infected with serotypes 1, 2, 8, or 9. Serum of a pig vaccinated with CSN of a serotype 3 strain also recognized the protease, but not sera of pigs vaccinated with a bacterin (serotype 1). Proteins from CSN of all the serotypes, which were precipitated with 70% (NH4)2SO4, were recognized by a polyclonal antibody raised against the purified protease. Taken together these results indicate that an antigenic protease is produced in vivo by all the serotypes of A. pleuropneumoniae. The results indicate that proteases could have a role in the disease and in the immune response of pigs infected with A. pleuropneumoniae.
Assuntos
Actinobacillus pleuropneumoniae/enzimologia , Endopeptidases/isolamento & purificação , Endopeptidases/metabolismo , Actinobacillus pleuropneumoniae/classificação , Animais , Cromatografia de Afinidade , Cromatografia DEAE-Celulose , Eletroforese em Gel de Poliacrilamida , Endopeptidases/química , Liofilização , Imunoglobulina A/metabolismo , Imunoglobulina G/metabolismo , Cinética , Peso Molecular , Sorotipagem , Especificidade por Substrato , Suínos , UltrafiltraçãoAssuntos
Antígenos de Bactérias , Brucella/isolamento & purificação , Brucelose/veterinária , Doenças dos Ovinos , Animais , Brucelose/diagnóstico , Estabilidade de Medicamentos , Eletroforese em Gel de Poliacrilamida , Epididimite/etiologia , Epididimite/microbiologia , Epididimite/veterinária , Imunodifusão/métodos , Masculino , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Sefarose , Testes Sorológicos/métodos , OvinosRESUMO
Gallibacterium anatis (previously named Pasteurella haemolytica-like) is considered a normal inhabitant of genital and upper respiratory tracts of healthy chickens, but it is also associated with different pathological conditions. Secreted metalloproteases from field and reference G. anatis cultures were obtained by methanol precipitation and were characterized. Proteins of molecular mass higher than 100 kDa showing proteolytic activity were observed in 10% polyacrylamide gels copolymerized with 1% bovine casein. They were active at alkaline pH, and inhibited by ethylenediamine tetraacetic acid. Their activity was stable at 50 degrees C, but partially inhibited at 60 degrees C, and totally inhibited at higher temperatures. Secreted proteins were able to degrade chicken IgG after 24 h of incubation, and cross-reacted with a polyclonal antibody against purified protease from Actinobacillus pleuropneumoniae. Secreted metalloproteases could play a role in infections caused by G. anatis.
Assuntos
Galinhas/microbiologia , Imunoglobulina G/metabolismo , Metaloproteases/metabolismo , Pasteurellaceae/metabolismo , Animais , Proteínas de Bactérias/imunologia , Proteínas de Bactérias/metabolismo , Galinhas/imunologia , Concentração de Íons de Hidrogênio , Metaloproteases/isolamento & purificação , TemperaturaRESUMO
The capability of Pasteurella multocida to secrete proteases to the culture medium and their characterization were studied in five animal isolates (bovine, chicken, sheep, and two from pig). All the isolates produced proteases in a wide range of molecular mass. It is suggested that they are neutral metalloproteases, since they were optimally active between pH 6 and 7, inhibited by chelating agents but not by other protease inhibitors, and reactivated by calcium. Proteases from isolates were able to degrade IgG. Several proteins from supernatants of cultures precipitated with 70% (NH4)2SO4 of all the P. multocida isolates were recognized by a polyclonal antiserum raised against a purified protease from Actinobacillus pleuropneumoniae. Protease production might play an important role during tissue colonization and in P. multocida diseases.