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Int J Mol Sci ; 25(9)2024 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-38732164

RESUMO

Cold atmospheric pressure plasma (CAP) offers a variety of therapeutic possibilities and induces the formation of reactive chemical species associated with oxidative stress. Mesenchymal stem/stromal cells (MSCs) play a central role in tissue regeneration, partly because of their antioxidant properties and ability to migrate into regenerating areas. During the therapeutic application, MSCs are directly exposed to the reactive species of CAP. Therefore, the investigation of CAP-induced effects on MSCs is essential. In this study, we quantified the amount of ROS due to the CAP activation of the culture medium. In addition, cell number, metabolic activity, stress signals, and migration were analyzed after the treatment of MSCs with a CAP-activated medium. CAP-activated media induced a significant increase in ROS but did not cause cytotoxic effects on MSCs when the treatment was singular and short-term (one day). This single treatment led to increased cell migration, an essential process in wound healing. In parallel, there was an increase in various cell stress proteins, indicating an adaptation to oxidative stress. Repeated treatments with the CAP-activated medium impaired the viability of the MSCs. The results shown here provide information on the influence of treatment frequency and intensity, which could be necessary for the therapeutic application of CAP.


Assuntos
Pressão Atmosférica , Movimento Celular , Meios de Cultura , Células-Tronco Mesenquimais , Estresse Oxidativo , Gases em Plasma , Espécies Reativas de Oxigênio , Células-Tronco Mesenquimais/metabolismo , Células-Tronco Mesenquimais/efeitos dos fármacos , Células-Tronco Mesenquimais/citologia , Humanos , Gases em Plasma/farmacologia , Movimento Celular/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismo , Meios de Cultura/química , Meios de Cultura/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Células Cultivadas , Sobrevivência Celular/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos
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