1.
Org Biomol Chem
; 1(23): 4144-7, 2003 Dec 07.
Artigo
em Inglês
| MEDLINE
| ID: mdl-14685317
RESUMO
A truncated version of the spinosyn polyketide synthase comprising the loading module and the first four extension modules fused to the erythromycin thioesterase domain was expressed in Saccharopolyspora erythraea. A novel pentaketide lactone product was isolated, identifying cryptic steps of spinosyn biosynthesis and indicating the potential of this approach for the biosynthetic engineering of spinosyn analogues. A pathway for the formation of the tetracyclic spinosyn aglycone is proposed.