RESUMO
The mechanisms of the P. oxalicum SL2-mediated microbial community on phosphorus solubilization and Pb stabilization were investigated through a 90-day soil experiment. In the treatments inoculated with P. oxalicum SL2, the amount of P. oxalicum SL2-GFP remained at 77.8%-138.6% of the initial inoculation amount after 90 days, and the available phosphorus (AP) content increased 21.7%-40.8% while EDTA-Pb decreased 29.9%-43.2% compared with CK treatment. SEM-EDS results showed that P. oxalicum SL2 changed the agglomeration degree of microaggregates and promoted the combination of Pb with C and O elements. These phenomena were enhanced when applied with Ca3(PO4)2. Microbial community analysis showed that P. oxalicum SL2 improved soil microbial activity, in which the fungi absolute abundance increased about 15 times within 90 days. Correlation analyses and a partial least-squares path model showed that the activation of Penicillium, Ascobolus, Humicola, and Spizellomyces in a fungal community increased the content of oxalate and AP, which directly decreased EDTA-Pb content, while the change of Bacillus, Ramlibacter, Gemmatimonas, and Candidatus Solibacter in the bacterial community regulated Fe/Mn/S/N cycle-related functions, thus promoting the conversion of Pb to oxidizable state. Our findings highlight that P. oxalicum SL2 enhanced the microbial-induced phosphate precipitation process by activating soil microbial communities and regulating their ecological functions.
Assuntos
Penicillium , Fósforo , Chumbo , Solo , Ácido EdéticoRESUMO
Nanoscale zero-valent iron (nZVI) faces significant challenges in Cr(VI) remediation through aggregation and passivation. This study identified a Cr(VI)-resistant filamentous fungus (Penicillium oxalicum SL2) for nZVI activation and elucidated the synergistic mechanism in chromium remediation. P. oxalicum SL2 and nZVI synergistically and effectively removed Cr(VI), mainly by extracellular nonenzymatic reduction (89.1%). P. oxalicum SL2 exhibited marked iron precipitate solubilization and Fe(II) regeneration capabilities. The existence of the Fe(II)-Cr(V)-oxalate complex (HCrFeC4O9) indicated that in addition to directly reducing Cr(VI), iron ions generated by nZVI stimulated Cr(VI) reduction by organic acids secreted by P. oxalicum SL2. RNA sequencing and bioinformatics analysis revealed that P. oxalicum SL2 inhibited phosphate transport channels to suppress Cr(VI) transport, facilitated iron and siderophore transport to store Fe, activated the glyoxylate cycle to survive harsh environments, and enhanced organic acid and riboflavin secretion to reduce Cr(VI). Cr(VI) exposure also stimulated the antioxidative system, promoting catalase activity and maintaining the intracellular thiol/disulfide balance. Cr(VI)/Fe(III) reductases played crucial roles in the intracellular reduction of chromium and iron, while nZVI decreased cellular oxidative stress and alleviated Cr(VI) toxicity to P. oxalicum SL2. Overall, the P. oxalicum SL2-nZVI synergistic system is a promising approach for regenerating Fe(II) while reducing Cr(VI).
Assuntos
Ferro , Poluentes Químicos da Água , Cromo/análise , Antioxidantes , Compostos Ferrosos , Poluentes Químicos da Água/análise , AdsorçãoRESUMO
Oxidation remediation is a commonly used technology for PAHs contaminated soil presently, but the overestimate of efficiency due to ongoing remediation by residual oxidants during extraction and testing has not been paid enough attention. In this study, persulfate was activated by Fe(II) to investigate the effects of residual oxidants on PAHs removal during detection process and the elimination effects of adding Na2SO3 and extending sampling time on residual oxidants. Results verified that the residual oxidants removed PAHs in extraction process, making the results lower than the actual values: the detection recovery rate η of ∑PAHs and 3-6 ring PAHs ranged from 24.3% (25% Na2S2O8 treatment) to 87.4% (5% Na2S2O8+4/4Fe2+ treatment), 20.1%-99.0%, 28.9%-87.9%, 20.8%-89.4%, and 18.6%-76.9%, respectively. After adding Na2SO3, the accuracy of detection results increased significantly: the η of ∑PAHs and 3-6 ring PAHs increased to 64.1%-96.5%, 58.8%-95.5%, 73.8%-114.4%, 60.6%-95.6%, and 45.4%-77.1%, respectively. After 49 days of adding oxidants, residual oxidants had no considerable effect on the detection of PAHs, indicating it was appropriate to start soil remediation verification sampling49 days after the remediation was completed. The observed results will help scientific evaluation of the remediation effects of chemical oxidation on organic contaminated soil.
Assuntos
Recuperação e Remediação Ambiental , Hidrocarbonetos Policíclicos Aromáticos , Poluentes do Solo , Hidrocarbonetos Policíclicos Aromáticos/análise , Compostos de Sódio , Solo , Poluentes do Solo/análise , SulfatosRESUMO
Penicillium oxalicum SL2 (SL2) is a previously screened Pb-tolerant fungus that can promote crops growth. The relationship between SL2 colonization and Pb immobilization was studied to provide a theoretical basis for microbial remediation of Pb-contaminated paddy soil. In this study, green fluorescent protein (GFP) labeled SL2 was inoculated into different Pb-contaminated paddy soils (S1-S6). The Pb extracted from the soil by HNO3, EDTA and CaCl2 were used to characterize the available Pb. The results showed that the colonization of SL2 was divided into lag phase (0-7 days), growth phase (7-30 days), and mortality phase (30-90 days). SL2 colonized well in sandy soils rich in clay and total phosphorus with initial pH of 4.5-7.0. In addition, SL2 increased soil pH and decreased soil Eh, which was beneficial to immobilize Pb. In different soils, the highest percentages of CaCl2-Pb, EDTA-Pb, and HNO3-Pb immobilized by SL2 were 34.34%-40.53%, 17.05%-20.11%, and 7.39%-15.62%, respectively. Pearson correlation analysis showed that the percentages of CaCl2-Pb and EDTA-Pb immobilized by SL2 were significantly positively correlated with the number of SL2 during the growth phase. SL2 mainly immobilized Pb in the growth phase and a higher peak number of SL2 was beneficial to the immobilization of Pb.
Assuntos
Poluentes do Solo , Solo , Cloreto de Cálcio , Ácido Edético , Chumbo/análise , Penicillium , Solo/química , Poluentes do Solo/análiseRESUMO
The exploration of microbial resources to reduce Pb accumulation in rice attracted great attention. In this study, we found Penicillium oxalicum SL2, a Pb-tolerant strain with good capability of dissolving phosphorus and stabilizing Pb in soil, was able to colonize on the root surface of rice seedlings without additional carbon sources, and promoted the secretion of metabolites related to amino acid metabolism, organic acid metabolism, signal transduction and other pathways in rhizosphere exudates, in which the secretion of oxalate increased by 47.7 %. However, P. oxalicum SL2 increased Fe(II) proportion and Fe availability on the root surface, resulting in iron plaque content decrease. Moreover, by converting root surface Pb from Pb-Fe state to PbC2O4 and Pb-P compounds, P. oxalicum SL2 increased Pb intercept capacity of iron plaque by 118.0 %. Furthermore, P. oxalicum SL2 regulated element distribution on the root surface, and reduced the relative content of Pb on the maturation zone of root tip, which was conducive to reducing Pb uptake by apoplastic pathway and the risk of Pb accumulation in root system. Our findings further revealed the interaction between P. oxalicum SL2 and rice root, providing a theoretical basis for the development and application of microbial agents in Pb-contaminated farmland.
Assuntos
Oryza , Penicillium , Poluentes do Solo , Ferro/análise , Chumbo/análise , Poluentes do Solo/análise , Solo/química , Raízes de Plantas/metabolismoRESUMO
Cd contamination in rice urgently needs to be addressed. Nano hydroxyapatite (n-HAP) is an eco-friendly material with excellent Cd fixation ability. However, due to its own high reactivity, innovative application of n-HAP in the treatment of Cd contamination in rice is needed. In this study, we proposed a new application, namely n-HAP pre-treatment, which can effectively reduce Cd accumulation in rice and alleviate Cd stress. The results showed that 80 mg/L n-HAP pre-treatment significantly reduced Cd content in rice shoot by 35.1%. Biochemical and combined transcriptomic-proteomic analysis revealed the possible molecular mechanisms by which n-HAP pre-treatment promoted rice growth and reduced Cd accumulation. (1) n-HAP pre-treatment regulated gibberellin and jasmonic acid synthesis-related pathways, increased gibberellin content and decreased jasmonic acid content in rice root, which promoted rice growth; (2) n-HAP pre-treatment up-regulated gene CATA1 expression and down-regulated gene OsGpx1 expression, which increased rice CAT activity and GSH content; (3) n-HAP pre-treatment up-regulated gene OsZIP1 expression and down-regulated gene OsNramp1 expression, which reduced Cd uptake, increased Cd efflux from rice root cells.
Assuntos
Oryza , Poluentes do Solo , Plântula , Cádmio/metabolismo , Proteômica , Giberelinas/farmacologia , Giberelinas/análise , Giberelinas/metabolismo , Poluentes do Solo/metabolismo , Raízes de Plantas/metabolismoRESUMO
Nowadays, Lead (Pb) and Cadmium (Cd) contamination in rice is an important worldwide environmental concern. Fe3O4 nanoparticles (Fe3O4 NPs) and Nano hydroxyapatite (n-HAP) are promising materials to manage Pb and Cd contamination. This study systematically investigated the effect of Fe3O4 NPs and n-HAP on Pb and Cd stressed rice seedlings' growth, oxidative stress, Pb and Cd uptake and subcellular distribution in roots. Furthermore, we clarified the immobilization mechanism of Pb and Cd in the hydroponic system. Fe3O4 NPs and n-HAP could reduce Pb and Cd uptake of rice mainly through decreasing Pb and Cd concentrations in culture solution and combining with Pb and Cd in root tissues. Pb and Cd were immobilized by Fe3O4 NPs through complex sorption processes and by n-HAP through dissolution-precipitation and cation exchange, respectively. On the 7th day, 1000 mg/L Fe3O4 NPs reduced the contents of Pb and Cd in shoots by 90.4% and 95.8%, in roots by 23.6% and 12.6%, 2000 mg/L n-HAP reduced the contents of Pb and Cd in shoots by 94.7% and 97.3%, in roots by 93.7% and 77.6%, respectively. Both NPs enhanced the growth of rice seedlings by alleviating oxidative stress and upregulating glutathione secretion and antioxidant enzymes activity. However, Cd uptake of rice was promoted at certain concentrations of NPs. The subcellular distribution of Pb and Cd in roots indicated that both NPs decreased the percentage of Pb and Cd in the cell wall, which was unfavorable for Pb and Cd immobilization in roots. Cautious choice was needed when using these NPs to manage rice Pb and Cd contamination.
Assuntos
Nanopartículas , Oryza , Poluentes do Solo , Plântula , Cádmio/análise , Chumbo/toxicidade , Antioxidantes/farmacologia , Raízes de Plantas/química , Poluentes do Solo/farmacologiaRESUMO
Excess chromium (Cr) and arsenic (As) coexist in soil such as chromated copper arsenate (CCA) contaminated sites, leading to high risks of pollution. Fe-biochar with adjustable redox activity offers the possibility of simultaneous stabilization of Cr and As. Here, a series of Fe-biochar with distinct Fe/C structure were rationally produced for the remediation of Cr and As contaminated soil (BCX-Fe, X represented the biomass/Fe ratio). Adsorption tests showed that maximal adsorption of BC5-Fe for Cr(VI) and As(III) reached 73.7 and 81.3 mg/g. A 90-day soil remediation experiment indicated that the introduction of 3% (w/w) Fe-biochar reduced the leaching state of Cr(VI) by 93.8-99.7% and As by 75.2-95.6%. Under simulated groundwater erosion for 10 years and acid rain leaching for 7.5 years, the release levels of Cr(VI) and As in the BC5-Fe remediated soil could meet the groundwater class IV standard in China (Cr(VI)<0.1 mg/L, As<0.05 mg/L). Accelerated aging tests demonstrated that BC5-Fe had long-term Cr and As stabilization ability. The quenching experiment, EPR, and XPS suggested that the corrosion products of Fe dominated the adsorption and redox reactions, while the O groups acted as electron transfer stations and constituted redox microcirculation in the synchronous uptake of Cr/As. Based on these insights, we believe that our study will provide meaningful information about the application potential of Fe-biochar for the heavy metal contaminated soil remediation.
Assuntos
Arsênio , Poluentes do Solo , Solo , Carvão Vegetal/química , Cromo/análise , Poluentes do Solo/análise , AdsorçãoRESUMO
The validity of USEPA Method 3060A as universal Cr(VI) analysis method for remediated soil is controversial. We investigated soil Cr(VI) remediation performance by commonly used reductants (FeSO4, CaSx, Na2S) under different operating conditions (dosage, curing time and degree of mixing) using Method 3060A, and developed modified 3060A specific for sulfide-based reductants. Results showed that Cr(VI) was primarily removed during analysis stage rather than remediation stage. Thereinto, chemical dosage played a much more important role than curing time and degree of mixing. Besides, soil Cr(VI) concentration decreased to below the detection limit with residual reductant content increasing. Comparing standard and toluene-mercuric modified 3060A, Cr(VI) removal efficiency decreased from 100 % to 38.9-45.4 %, 67.1-68.8 % and 94.1-96.3 %, corresponding to mixing degree of 33 %, 67 % and 100 %, for treated soil using 1× and 2× the molar stoichiometric ratio of CaSx. Subsequently, the optimization mechanism was revealed. Elemental sulfur, remediation product of sulfide-based reductants, was removed from soil by toluene preventing its disproportionation to sulfide at Method 3060A stage. Sulfide was fixed by mercuric oxide in species of mercuric sulfide. This method also proved suitable for different types of soils. Therefore, an effective way for scientific evaluation of soil Cr(VI) remediation was provided in this study.
RESUMO
Cadmium (Cd) contamination in paddy soil has become a worldwide concern and severely endangered human health. Nano hydroxyapatite (n-HAP) is a practical material to manage paddy Cd pollution, but its dosage should not be excessive. Based on previous studies, we validated the effect of n-HAP pre-treatment on rice Cd uptake in pot and field experiments. The results indicated that n-HAP pre-treatment effectively restricted Cd translocation in the soil-rice system. In pot experiment, when soil n-HAP concentration was 5000 mg/kg, the Cd content in the grains of n-HAP pre-treated rice was 0.171 mg/kg, decreased by 29.3% compared with normal rice (0.242 mg/kg). In field experiment, when soil n-HAP concentration was 20,000 mg/kg, the Cd content in the grains of n-HAP pre-treated rice was 0.156 mg/kg, decreased by 35.3% compared with normal rice (0.241 mg/kg). The primary mechanism was that n-HAP pre-treatment altered the formation and composition of iron plaque and therefore enhanced the Cd binding ability of iron plaque. The available N and P content and urease activity in paddy field were increased. We further investigated the impact of n-HAP on the diversity and structure of paddy microbial communities. The Chao1 and Shannon diversity indices showed no significant difference. The relative abundance of Actinobacteria and Proteobacteria was significantly decreased by n-HAP, indicating that Cd pollution might be alleviated. Desulfobacterota, Gemmatimonadota, and Geobacteraceae were significantly enriched by n-HAP. The declining relative abundance of Basidiomycota and the increasing relative abundance of other fungal taxa also suggested that n-HAP could alleviate Cd toxicity in soil.
Assuntos
Oryza , Poluentes do Solo , Humanos , Cádmio/análise , Oryza/química , Poluentes do Solo/análise , Ferro/metabolismo , Solo/química , Bactérias/metabolismoRESUMO
As a potential bioremediation strain for Pb contamination, Penicillium oxalicum SL2 sometimes has secondary activation of Pb, so it is crucial to clarify its effect on Pb morphology and its intracellular response to Pb stress. We investigated the effect of P. oxalicum SL2 in medium on Pb2+ and Pb availability in eight minerals, and revealed the prioritization of Pb products. (i)Pb was stabilized within 30 days as Pb3(PO4)2 or Pb5(PO4)3Cl with sufficient phosphorus (P); (ii) under P deficiency but sulfur (S) sufficient, Pb was stabilized mainly in the form of PbSO4; (iii) under conditions of P and S deficiency, Pb was stabilized mainly in the form of PbC2O2. With the help of proteomic and metabolomics analysis, a total of 578 different proteins and 194 different metabolites were found to be matched in 52 pathways. Among them, the activation of chitin synthesis, oxalate production, sulfur metabolism and transporters improved the Pb tolerance of P. oxalicum SL2, and promoted the synergistic effect of extracellular adsorption, bio-precipitation and transmembrane transport on Pb stabilization. Our results fill the gap in the intracellular response of P. oxalicum SL2 to Pb and provide new insights into the development of bioremediation agent and technology for Pb contamination.
Assuntos
Biodegradação Ambiental , Chumbo , Penicillium , Adsorção , Chumbo/metabolismo , Proteômica , Enxofre/metabolismoRESUMO
The toxic chemical element cadmium (Cd) in paddy fields triggered increasing problems of growth inhibition and food security in rice consistently. In this study, we found Metarhizium robertsii, which is widely used as a bioinsecticide and biofertilizer in agriculture and recently found to be resistant to Cd, developed intraradical and extraradical symbiotic hyphae in rice seedlings, and successfully colonized in the rice rhizosphere soil to more than 103 CFUs g-1 soil at harvesting. M. robertsii colonization significantly reduced Cd accumulations in both hydroponically cultured seedlings and the matured rice cultured in Cd contaminated potting soil (2 ppm). Notably, Cd accumulation reduction of the roots, stems, leaves, husks and grains of the matured rice induced by the fungus were 44.3%, 32.1%, 35.3%, 31.9% and 24.7%, respectively. It was caused by the M. robertsii-induced suppression of Cd intake transporter gene osNramp5 in the rice roots, and the chemical stabilizing of Cd to the residual fraction in the rhizosphere soil. In addition, the colonization of M. robertsii significantly promoted the growth characters and the photosynthesis of the rice plants. This is achieved by the increase of endogenous hormone levels of indole-3-acetic, gibberellin A3 and brassinolide induced by M. robertsii. Furthermore, the fungus enhanced the antioxidative capacities via increasing enzyme activities of catalase, peroxidase and the production of glutathione, ascorbic acid, proline in the rice plants. Our work provides theoretical basis for expanding the use of M. robertsii as in situ Cd accumulation reduction and detoxification agents for rice in contaminated paddy fields.
Assuntos
Oryza , Poluentes do Solo , Cádmio/análise , Metarhizium , Oryza/genética , Raízes de Plantas/química , Plântula , Solo , Poluentes do Solo/análiseRESUMO
Respiratory monitoring and contactless sensing using the moisture produced by respiration and perspiration have garnered considerable attention in recent years. In this study, we fabricated polyimide-sputtered and polymerized (PSP) humidity sensors with ultrahigh capacitive sensitivity, fast response, and a wide working range of relative humidity (RH). The sensors produced >40â¯000 times increment in the sensing signal over the 10-95% RH range at 10 Hz and exhibited good performance at low RH levels (<40%) as well. These sensors displayed excellent sensing properties with small hysteresis, long-time stability, and fast response and recovery times (2.4 and 1.2 s, respectively). In the mechanism study of PSP humidity sensors, we found that the high sensitivity can be attributed to massive hydrophilic functional groups formed on the polymer chains by moist aging with oxidation and the fast response speed is due to the mesoporous structure of PSP films. We also fabricated a 5 × 5 array of PSP humidity sensors to identify the shapes of wet objects and of leaves during transpiration. Thus, we reported a novel and effective method for fabricating high-performance humidity polymer films, channeling new pathways for the development of advanced humidity and gas sensors.
RESUMO
Heavy metal contamination in agricultural soil and its associated risk of food safety are of great concern globally. It is therefore an urgent need to develop sustainable option to mitigate the accumulation of metals in crop plants. Here we investigated the potential of phosphorus-solubilizing fungus, Penicillium oxalicum SL2, on regulating the bioavailability of Pb in a lead (Pb) polluted soil-rice system. Our results showed that the content of Pb in rice grain was significantly decreased by ~80% with the application of P. oxalicum SL2. The competition between oxalate and phosphate for the complexation of Pb showed to be effective in mediating the bioavailability of Pb, and such impact varied with water fluctuation in paddy soil. The solubilization of phosphorus also played an important role in alleviating the dissolution of iron plaque caused by oxalic acid, which helped maintaining the biomass of iron plaque as a barrier to the uptake of Pb by root. The predominant indigenous microbial community was not affected by the inoculation with P. oxalicum SL2, suggesting it as an eco-friendly strain. Therefore, we suggest P. oxalicum SL2 as a promising fungus in enhancing the safe use of moderately Pb polluted paddy soil for safe rice.
Assuntos
Oryza , Penicillium , Poluentes do Solo , Cádmio/análise , Chumbo , Solo , Poluentes do Solo/análiseRESUMO
Due to the high migration capacity in agricultural soil-crop systems, cadmium (Cd) is accumulated in various crops and severely inhibits plant growth. In this study, we showed that, under Cd stress, the plant-symbiotic fungus Metarhizium robertsii reduced Cd accumulation in Arabidopsis thaliana shoots and roots by 21.8 % and 23.8 %, respectively. This is achieved by M. robertsii colonization-induced elevation of Cd efflux capacity via upregulation of three PCR genes, which is confirmed by the fact that the extent to which M. robertsii reduced Cd accumulation in the WT plants was greater than the inactivating mutants of the PCR genes. M. robertsii also alleviated Cd-induced leaf etiolation in A. thaliana by increasing the chlorophyll amount and modified plant physiological status to increase Cd stress tolerance via increasing production of catalase, peroxidase and glutathione and upregulating multiple HIPP proteins involved in sequestration of Cd. Notably, consistent with that in A. thaliana, the colonization of M. robertsii also reduced the Cd accumulation in Oryza sativa seedlings by upregulating the PCR gene OsPCR1, and increased chlorophyll amount and alleviated oxidative stress. Therefore, M. robertsii colonization reduced Cd accumulation in plants, and promoted plant growth and health by elevating Cd efflux capacity and modifying physiological status.
Assuntos
Arabidopsis , Oryza , Antioxidantes/metabolismo , Arabidopsis/metabolismo , Cádmio/metabolismo , Cádmio/toxicidade , Clorofila/metabolismo , Metarhizium , Oryza/metabolismo , Estresse Oxidativo , Raízes de Plantas/metabolismo , Plântula/metabolismo , Estresse FisiológicoRESUMO
Lead (Pb) and arsenic (As) exist in soil with different ionic forms, and it is difficult to immobilize simultaneously Pb and As in soil. The objective of this study is to determine the effects of water management including flooding (FL), alternate wetting and drying (AWD) and dry farming (DF) combined with addition of phosphate (P) on the accumulation of Pb and As in rice. Our results showed that Pb accumulated in root during vegetative stage, and most of As in root was transported to the above ground parts during the reproductive stage. Pb was evenly distributed in grains, and As was mostly accumulated in bran and aleurone layer. Water management had a reverse effect on the accumulation of Pb and As in rice. However, the effects of P on arid soil environment and Pb, As accumulation in rice were stronger than that in flooded soil. Application of P under AWD treatment could maintain a similar quantity of Fe plaque with flooding, decrease the availability of Pb in rhizosphere soil, reduce Pb and As accumulation in root, and result in the reduction of Pb and As accumulation in grains by 86% and 66% respectively. Besides, our study also found that flooding or AWD during vegetative stage facilitated the formation of iron plaque. In conclusion, AWD combined with P application could maintain a relatively lower concentrations of Pb and As in grains.
Assuntos
Arsênio , Oryza , Poluentes do Solo , Arsênio/análise , Chumbo , Fosfatos , Solo , Poluentes do Solo/análise , Água , Abastecimento de ÁguaRESUMO
Passivation of nanoscale zerovalent iron hinders its efficiency in water treatment, and loading another catalytic metal has been found to improve the efficiency significantly. In this study, Cu/Fe bimetallic nanoparticles were prepared by liquid-phase chemical reduction for removal of hexavalent chromium (Cr(VI)) from wastewater. Synthesized bimetallic nanoparticles were characterized by transmission electron microscopy, Brunauer-Emmet-Teller isotherm, and X-ray diffraction. The results showed that Cu loading can significantly enhance the removal efficiency of Cr(VI) by 29.3% to 84.0%, and the optimal Cu loading rate was 3% (wt%). The removal efficiency decreased with increasing initial pH and Cr(VI) concentration. The removal of Cr(VI) was better fitted by pseudo-second-order model than pseudo-first-order model. Thermodynamic analysis revealed that the Cr(VI) removal was spontaneous and endothermic, and the increase of reaction temperature facilitated the process. X-ray photoelectron spectroscopy (XPS) analysis indicated that Cr(VI) was completely reduced to Cr(III) and precipitated on the particle surface as hydroxylated Cr(OH)3 and CrxFe1-x(OH)3 coprecipitation. Our work could be beneficial for the application of iron-based nanomaterials in remediation of wastewater.
RESUMO
Lead (Pb) is a typical toxic heavy metal element in soils and plants, which has a potential threat to human health through the food chain. Uptake of Pb in the soil-vegetable system has attracted broad attention, whereas reports on the main controlling factors of Pb uptake and accumulation in different soil-vegetable systems are limited. The effect of soil properties on Pb uptake and accumulation in pepper (Capsicum annuum L.) was studied by a pot experiment with 16 typical soils in China. The results showed that the Pb bioavailability was lower in alkaline soils, and that soil cation exchange capacity (CEC), CaCO3 , and total phosphorus contents might influence the uptake and transfer of Pb by peppers. Soil pH and CEC were the most significant factors affecting Pb accumulation in pepper fruits. Soil pH was negatively correlated with Pb uptake and accumulation due to its influence on Pb mobility and bioavailability. The accumulation of Pb decreased as soil CEC increased, which might inhibit the absorption and transfer of Pb in peppers. The multiple linear regression function based on soil Pb content, pH, and CEC could provide enough information for a good prediction of the accumulation of Pb in soil-pepper systems (R2 = 0.733). The results are in favor of developing a Pb threshold for vegetables in agricultural soils in China, thus improving the food safety of crops. Environ Toxicol Chem 2021;40:1443-1451. © 2021 SETAC.
Assuntos
Metais Pesados , Poluentes do Solo , China , Humanos , Chumbo , Metais Pesados/análise , Solo , Poluentes do Solo/análise , VerdurasRESUMO
Though the interaction between humic acid (HA) and heavy metals has been widely reported, the effects of HA on the toxicity of heavy metals to plants are still in debate. In this study, the regulation mechanisms of HA on Pb stress in tea plant (Camellia sinensis L.) was investigated through hydroponic experiments, and the experimental results were explained by using transmission electron microscope (TEM), scanning transmission X-ray microscopes (STXM) and isobaric tags for relative and absolute quantitation (iTRAQ) differential proteomics. Significant alleviation of Pb stress was found with HA coexistence. TEM results showed that HA greatly mitigated the damage of cells caused by Pb stress. Compared with sole Pb treatment, the addition of HA increased the contents of pectin and pectic acid in the cell wall by 10.5% and 30.5%, while arabinose (Ara) and galactose (Gal) decreased by 20.5% and 15.9%, respectively, which were beneficial for increasing Pb adsorption capacity of the cell wall and promoting cell elongation. Moreover, iTRAQ differential proteomics analysis proved that HA strengthened the antioxidant system, promoted the synthesis of cell wall, and stabilized protein and sulfur-containing substance metabolism in molecular level. Notably, the concentration of calcium (Ca) in the cell wall of HA coexistence treatment was 47.4% higher than Pb treatment. STXM results also indicated that the distribution of Ca in the cell wall was restored with the presence of HA. This might promote the formation of the egg-box model, thus alleviating Pb stress in cells. Our results reveal the regulation mechanisms of HA on Pb detoxification in plants and provide useful information for improving the safety of agricultural products.