1.
Nucleosides Nucleotides Nucleic Acids
; 20(4-7): 1177-9, 2001.
Artigo
em Inglês
| MEDLINE
| ID: mdl-11562981
RESUMO
A new and efficient strategy for labeling of RNA sequences prior to their hybridization on high density DNA chip has been developed. Our approach which combines the fragmentation and the labeling is based on the reactivity of the 3'-phosphate of cleaved RNA fragments with a fluorescent molecule bearing aromatic bromomethyl function.
Assuntos
Análise de Sequência com Séries de Oligonucleotídeos , RNA/química , RNA Polimerases Dirigidas por DNA/química , RNA Polimerases Dirigidas por DNA/genética , Corantes Fluorescentes/química , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/química , RNA Ribossômico 16S/genética , Proteínas Virais
2.
Nucleosides Nucleotides Nucleic Acids
; 22(5-8): 1647-9, 2003.
Artigo
em Inglês
| MEDLINE
| ID: mdl-14565486
RESUMO
We show here a new and efficient aqueous chemistry for labeling of any class of nucleic acids for their detection on DNA chip. The labels contain a diazo function as reactive moiety and biotin as detectable unit. The highly selective reaction of diazo group on the phosphate does not disrupt base pairing recognition and hybridization specificity.