RESUMO
TDP-43 is an evolutionarily conserved ubiquitously expressed DNA/RNA-binding protein. Although recent studies have shown its association with a variety of neurodegenerative disorders, the function of TDP-43 remains poorly understood. Here we address TDP-43 function using spermatogenesis as a model system. We previously showed that TDP-43 binds to the testis-specific mouse acrv1 gene promoter in vitro via two GTGTGT-motifs and that mutation of these motifs led to premature transcription in spermatocytes of an otherwise round spermatid-specific promoter. The present study tested the hypothesis that TDP-43 represses acrv1 gene transcription in spermatocytes. Plasmid chromatin immunoprecipitation demonstrated that TDP-43 binds to the acrv1 promoter through GTGTGT motifs in vivo. Reporter gene assays showed that TDP-43 represses acrv1 core promoter-driven transcription via the N-terminal RRM1 domain in a histone deacetylase-independent manner. Consistent with repressor role, ChIP on physiologically isolated germ cells confirmed that TDP-43 occupies the endogenous acrv1 promoter in spermatocytes. Surprisingly, however, TDP-43 remains at the promoter in round spermatids, which express acrv1 mRNA. We show that RNA binding-defective TDP-43, but not splice variant isoforms, relieve repressor function. Transitioning from repressive to active histone marks has little effect on TDP-43 occupancy. Finally, we found that RNA polymerase II is recruited but paused at the acrv1 promoter in spermatocytes. Because mutation of TDP-43 sites caused premature transcription in spermatocytes in vivo, TDP-43 may be involved in pausing RNAPII at the acrv1 promoter in spermatocytes. Overall, our study shows that TDP-43 is a transcriptional repressor and that it regulates spatiotemporal expression of the acrv1 gene during spermatogenesis.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Regulação da Expressão Gênica/fisiologia , Proteínas de Membrana/biossíntese , Proteínas Repressoras/metabolismo , Espermátides/metabolismo , Espermatócitos/metabolismo , Espermatogênese/fisiologia , Testículo/metabolismo , Animais , Proteínas de Ligação a DNA/genética , Histonas/genética , Histonas/metabolismo , Masculino , Proteínas de Membrana/genética , Camundongos , Mutação , Estrutura Terciária de Proteína , RNA Polimerase II/genética , RNA Polimerase II/metabolismo , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Proteínas Repressoras/genética , Elementos de Resposta/fisiologia , Espermátides/citologia , Espermatócitos/citologia , Testículo/citologia , Transcrição Gênica/fisiologiaRESUMO
Testis-specific promoters are unique in that relatively short proximal promoters of several genes have been shown to be capable of directing tissue- and cell-type-specific expression in transgenic mice. How such small promoter fragments perform the dual functions of maintaining a silenced state in somatic tissues and activating gene expression in the correct germ-cell type in testis remains poorly understood. Studies from our laboratory using the round spermatid-specific SP-10 gene as an experimental model have provided some insights into the mechanisms involved. It was found that the proximal promoter of the SP-10 gene acts as a chromatin insulator or boundary element in somatic tissues and prevents transcription of the SP-10 gene. In round spermatids, the insulator function is relieved, thus facilitating the SP-10 gene transcription. Insulators act as enhancer blockers and/or barriers to heterochromatin to protect the programmed expression of a gene. Typically, insulators are separable from promoters. In the case of the SP-10 gene, however, the insulator overlaps the promoter and operates in a facultative manner. We hypothesize that the proximal promoters of some testis-specific genes have adapted the insulator function to maintain transcriptional silence in the somatic tissues.