RESUMO
It was shown on an experimental model of chronic gastric ulcer in rats that administration of microsomal oxidation inductors benzonal (benzobarbital) and phenobarbital in a dose of 50 mg/kg for 10 days significantly increases the content of cytochrome P-450 in the gastric mucosa. The increased content of P-450 promotes stimulation of the synthesis of mucosal barrier glycoproteins. The cytoprotective effect of famotidine in a dose of 100 mg/kg and sucralfate in a dose of 400 mg/kg proved to be much weaker than that of the inductors.
Assuntos
Mucosa Gástrica/efeitos dos fármacos , Úlcera Gástrica/tratamento farmacológico , Animais , Antiulcerosos/uso terapêutico , Barbitúricos/uso terapêutico , Doença Crônica , Sistema Enzimático do Citocromo P-450/biossíntese , Sistema Enzimático do Citocromo P-450/efeitos dos fármacos , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Quimioterapia Combinada , Indução Enzimática/efeitos dos fármacos , Famotidina/uso terapêutico , Mucosa Gástrica/enzimologia , Masculino , Fenobarbital/uso terapêutico , Ratos , Estimulação Química , Úlcera Gástrica/enzimologia , Sucralfato/uso terapêuticoRESUMO
In patients with cirrhosis of the liver, there is noted a striking imbalance in the distribution of isoelectric fractions of serum albumin in the isoelectric field. Disproportion in isoelectric fractions of serum albumin can, we believe, be referred to functional loads on albumin relative to fixation and transport of surplus of bilirubin and ammonia. In the ascitic fluid, there comes to be seen, in fact, a reversal of imbalance of pH-dependent fractions of albumin,--probably, due to "purification" in the lymphatic system and partly in the functioning hepatocytes. As to the isoelectric spectrum, the ascitic fluid albumin is qualitatively very similar to serum albumin of healthy people.
Assuntos
Albuminas/análise , Cirrose Hepática/sangue , Adulto , Idoso , Albuminas/fisiologia , Líquido Ascítico/química , Eletroforese em Gel de Poliacrilamida , Feminino , Humanos , Concentração de Íons de Hidrogênio , Focalização Isoelétrica , Cirrose Hepática/metabolismo , Masculino , Pessoa de Meia-Idade , Albumina Sérica/análise , Albumina Sérica/fisiologiaRESUMO
The results of the comparative study on the contents and activity of the enzymes of the system of oxidases of mixed function in the lungs and liver in rabbits, the specific features of phospholipid spectrum in the supernatant and microsomal fractions of the lungs and liver tissues are presented. The same parameters were investigated in experimental chronic inflammatory diseases of the lungs. The degree of the damage of the monoxygenase capacity of the lungs and the degree of the liver involvement in the process were established. The peculiarities of the inhibitory action of cimetidine on the detoxication capacity of the lungs and liver and on the disorder of the reproduction of phospholipids in these organs were revealed. The substrate specificity of cimetidine in inhibition of microsomal NADPN-cytochrome-C-reductase both in the liver and in the lungs, the probability of blocking the synthesis of phospholipids at the level of phosphatide acid and citidinephosphatecholine were noted. The results obtained should be taken into consideration during drug therapy.