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Objective: Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with a significant impact on global health. The identification of non-invasive biomarkers for early detection and monitoring of OSCC remains crucial. Methods: A total of 100 subjects, comprising 50 patients with histopathologically confirmed OSCC and 50 age- and sex-matched healthy controls, were enrolled in the study. Salivary samples were collected from all participants and analyzed using enzyme-linked immunosorbent assays (ELISA) to measure IL-1 levels. Clinical data, including demographic information, smoking habits, and alcohol consumption, were obtained from patient records. Results: The mean salivary IL-1 level was significantly higher in OSCC patients compared to healthy controls (P < 0.001). Furthermore, subgroup analysis demonstrated that advanced stages of OSCC correlated with significantly elevated IL-1 levels when compared to early-stage OSCC (P < 0.05). Additionally, high salivary IL-1 levels were associated with a more aggressive tumor phenotype and poorer prognosis, as reflected by tumor size, lymph node metastasis, and overall survival (P < 0.01). Conclusion: This case-control study provides compelling evidence that salivary Interleukin-1 (IL-1) levels are significantly elevated in patients with OSCC.
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BACKGROUND: Forensic dentistry is a subspecialty of forensic science that handles, examines, and presents evidence from teeth in the most ethical manner possible. In addition to doing research, forensic odontology (FO) entails managing, examining, assessing, and presenting evidence from dentistry in civil or criminal investigations. In these circumstances, the forensic odontologist aids the court system by reviewing the dental findings. AIM: The present investigation was carried out to assess the dental faculty member's awareness, interest, and knowledge of FO at dental educational institutions in Jharkhand, India. METHODS AND MATERIALS: The sample size calculation assumes a simple random sampling technique and a large population size using the formula E=sqrt((Z^2*p*(1-p))/n). A total of 102 dental teaching faculty members from diverse dental specialties participated in the survey. Utilizing a validated questionnaire that was sent directly after receiving approval from the institutional ethics committee in January 2023, data were gathered in a tailored manner. The questionnaire included 12 questions to gauge the dental teaching faculty members' awareness, knowledge, and interest in FO. Closed-ended questions were included. The outcomes were computed using a percentage system. RESULTS: In this study, 95% of study participants agreed with the fact that teeth are a possible source of DNA, while 5% were unaware of this fact. 68% of study participants said that visual examination constitutes the initial stage in the identification process for unidentified bodies while 8% of study participants said that DNA fingerprinting constitutes the initial stage in the identification process for unidentified bodies. However, 8% of study participants didn't know anything about this aspect. 72% of dental teaching faculty members agreed to the fact that Barr bodies should be used to determine sex. 89% of study participants responded positively to the question "Can teeth or enamel serve as a tool for determining age?" 11% of study participants did not know that teeth or enamel can serve as a tool for determining age. 41% of study participants had the correct information that FO instruction for bachelor of dental surgery (BDS) students takes place and, as per Dental Council of India (DCI) standards, should be given in both BDS second and third years. CONCLUSION: The results of the current survey provided information on FO practices among Jharkhand dental institutes' dental faculty. The poll revealed that they have the necessary information, which they must have learned either while studying, participating in continuing dental education, or teaching.
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The expression analysis of cyclin D1, Ki-67, MCM3 and MCM2 in oral squamous cell carcinoma to identify biomarkers is of interest. 45 formalin-fixed paraffin embedded tissue blocks collected from archives of Department of Oral and Maxillofacial Pathology and Oral Microbiology, Government Dental College and Hospital, Jamnagar, India were subjected to a retrospective cross-sectional immuno-histo-chemistry examination. 30 blocks of OSCC with histological diagnosis have 15 tissue blocks of well-differentiated oral carcinoma and 15 tissue blocks of moderately-differentiated oral carcinoma. 15 specimens of normal oral mucosa (NM) were also examined for comparison. In each of the categories, the immuno-histo-chemistry expression of cyclin D1, MCM 3, MCM 2, and ki67 was studied. Data shows that cyclin D1, Ki-67, MCM3 and MCM2 effectively indicate cellular proliferation for consideration as potential biomarkers of oral squamous cell carcinoma.
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BACKGROUND: The minor salivary glands (MSGs) are critical components of the mouth's delicate environment. The pre-malignant changes of oral submucous fibrosis (OSMF) have been associated with a decline in the quality of life and an uptick in the prevalence of oral malignancies. AIM: The aim was to provide evidence of the histological alterations in minor salivary gland structure seen in individuals with OSMF. METHODS AND MATERIALS: A total of 106 confirmed cases of OSMF were enrolled in the study. In order to perform an incisional biopsy, we first collected the patient's complete demographic and clinical history. Using a Vernier calliper, the inter-incisal distance was used to evaluate the mouth opening of the patients. An incisional biopsy of the buccal mucosa was carried out using a 6 mm diameter punch and local anaesthesia. After the appropriate demographic and medical information had been gathered. Acinar cells and the surrounding stroma of tissue slices were observed under a light microscope for alterations. The cytoplasm, nucleus morphology, cellular shape, mucin pooling, and acinar outline of acinar cells were all examined by the researchers. It was taken into account to classify OSMF histologically based on variations in juxta epithelial hyalinization. RESULTS: Multiple aetiologies for the symptoms of OSMF were reflected in the patient's histological abnormalities in the minor salivary glands. On the measurement of the diameter of acini, we discover that the average area of salivary gland acini in OSMF patients is smaller than in the normal group indicative of a decrease in size. The number of functional acini in OSMF is fewer than compared in the control group. CONCLUSION: Because of the findings of this study, we now have a better understanding of the factors that play a role in the incidence of dryness of the oral and pharyngeal mucosa (OSMF), although it has to be mentioned that no major impact of OSMF on minor salivary glands was observed in our study.