RESUMO
With Percoll density gradients, blasts from peripheral blood and bone marrow could be separated with a significant enrichment, and very often with a high degree of purity. This allowed a study of selected cases, where the separated sample exhibited chromosome abnormalities and/or an abnormal DNA content distribution (as measured by DNA-flow cytometry). The anomalies were shown to be associated with the separated blast fraction.
Assuntos
Aberrações Cromossômicas , DNA/análise , Citometria de Fluxo , Leucemia Linfoide/genética , Leucemia Mieloide/genética , Mielofibrose Primária/genética , Medula Óssea/ultraestrutura , HumanosRESUMO
In general bacterial contamination of enteral feeding solutions is considered to be exogenous in origin. However, during feeding through catheter jejunostomy another source of contamination seems possible. We describe a patient in whom bacterial contamination of the feeding solution given through a catheter jejunostomy occurred frequently. Microbiological evaluation of several feeding samples showed that with this particular type of catheter enterostomy contamination was dual in origin. A not aseptic manipulation of the feed prior to administration led only to slight contamination. Most of the contamination however was endogenous--ie. ascending from the gut of the patient. In contrast to some previous studies, no clinical or laboratory deterioration could be observed in relation to the contamination. The food value of the feed expressed as the glucose concentration was significantly reduced after contamination with the causative organisms.