Causes of admission and outcomes of brown hare (Lepus europaeus) leverets at wildlife rescue centres in the Czech Republic.

BACKGROUND: Wildlife rescue centres care for orphaned and injured young as an integral part of their work. However, inappropriate interventions in nature can have a negative effect on the survival of young hares, especially when the care of these young is not very successful. The aim of this study was to assess the number of brown hare leverets admitted to rescue centres in the Czech Republic in the period from 2010 to 2019, the causes of their admission to rescue centres and their outcomes. RESULTS: We evaluated the number of brown hare leverets admitted to rescue centres in the Czech Republic in the period from 2010 to 2019 and the outcomes associated with their leaving these rescue centres. We found that the number of brown hare leverets admitted increased during the monitored period (rSp = 0.6364, p < 0.05). The most frequent reasons for admission were the admission of orphaned young (49.15%), leverets brought needlessly (19.60%) and leverets that had been bitten by other animals (18.63%). More (p < 0.05) young admitted to rescue centres died (40.76%) than were reared successfully and released back into the wild (32.40%). Leverets that had been caught needlessly or orphaned and late-born leverets survived and could be released back into the wild (38.56, 34.51 and 52%, respectively), while fatalities were recorded in most leverets bitten by another animal (65.05%) or hit in a collision with a vehicle (97.06%). Most young hares (76.92%) that were exhausted or starved at the time of admission could not be saved. CONCLUSIONS: Since only a small proportion of hares in a litter survive until adulthood in the wild, young animals being found and taken needlessly to rescue centres may harm the hare population. Our results show that only around one in three healthy young hares admitted to rescue centres are reared successfully. It is, in our opinion, of fundamental importance to the protection of brown hare leverets to inform the public of this issue and prevent needless interventions into natural rearing in the wild.

Sodium chloride treatment effects on rainbow trout suffering from proliferative kidney disease caused by Tetracapsuloides bryosalmonae.

The aim of the present study was to evaluate the effect of a long-term sodium chloride bath on rainbow trout Oncorhynchus mykiss naturally infected by Tetracapsuloides bryosalmonae. A total of 106 infected fish were divided into 2 groups. One group was left untreated and the other was treated with sodium chloride in increasing doses up a concentration of 0.8%. After 14 d, treatment was stopped and for a further 7 d the fish response to the sodium chloride bath was observed. Cumulative mortality was significantly lower in the treated group (19.2%) compared to the untreated group (31.5%) after 21 d. This corresponded to the lower but non-significant parasite intensity in kidney and spleen in the treated group after 14 d of treatment. However, lower prevalence of parasites in both tissues was recorded in the untreated group after 21 d of treatment, but a significant difference was observed only in spleen tissue. Furthermore, significant increases in leukocytes, hemoglobin, haematocrit, ferric reducing ability of plasma, and ceruloplasmin, and significant decreases in alanine aminotransferase, aspartate aminotransferase and lactate dehydrogenase activities were noticed in the treated group compared to the untreated group. In contrast, significant decreases in lysozyme concentration in the mucus and phagocyte oxidative burst in the blood were observed in the treated group. Histopathological examination revealed proliferative and reparative changes in parenchymatous tissues in the treated group. The 14- and 21-d salt bath used in rainbow trout with proliferative kidney disease was associated with a reduction in mortality and enhanced the reparative phase in the treated group.

Accumulation of Mercury in The Tissues of the Great Cormorant (Phalacrocorax carbo) From Common Carp.

The aim of this work is to assess mercury content in the great cormorant in the Trebon region pond systems (Czech Republic) in terms of its potential to accumulate mercury from common carp. Selected tissues samples were taken from 51 cormorants and 30 common carp. In the food chain the cormorant was found to have the potential to accumulate mercury, where the muscle total mercury was roughly 35 times higher compared to the total mercury content in the carp muscle as its food. A statistically significantly higher overall mercury content (p < 0.01) has been found in the kidney and liver (2.23 ± 0.30, 2.12 ± 0.22 mg/kg) compared to other tissues examined in cormorants. The proportion of muscle methylmercury in the total mercury content of the cormorant was within the range 64.3%-87.3%. The results can help us to gain a better understanding of how mercury is distributed and accumulated in the aquatic food chain.

Transport losses in finisher pigs: impact of transport distance and season of the year.

OBJECTIVE: The death of animals during transport for slaughter is a major factor indicating the level of welfare in transported animals. The aim of this study was to assess mortality related to the commercial transport of finisher pigs for slaughter in the Czech Republic. METHODS: The inspectors of the State Veterinary Administration of the Czech Republic recorded the numbers of finisher pigs transported to processing plants in the Czech Republic for slaughter and the mortality in these pigs in relation to transport in the period from 2009 to 2014. RESULTS: Our results show that the likelihood of death losses in transported pigs increases with increasing transport distance. The transport-related mortality ranged from 0.049% in pigs transported for distances below 50 km to 0.145% in pigs transported for distances exceeding 300 km. The impact of external air temperature on the transport-related mortality found in our study clearly shows that current transport practices fail to ensure the welfare of pigs transported under other than moderate weather. Particularly cold temperatures below -2°C were associated with increased death losses in winter transport. CONCLUSION: Despite a decreasing trend in the mortality of finisher pigs transported for slaughter in Europe, our study suggests that current transport conditions are not efficient at ensuring the welfare of pigs during transport for longer distances and the protection of pigs against the negative impact of extreme ambient temperatures. Further research should focus on developing practical guidelines to improve the welfare of pigs in transit accordingly.

The Effects of Salicylic Acid on Juvenile Zebrafish Danio rerio Under Flow-Through Conditions.

The aquatic environment is becoming increasingly contaminated with pharmaceuticals. Salicylic acid (SA), which can be used individually or appear as a degradation product of the widely used acetylsalicylic acid was chosen for testing. Juvenile zebrafish Danio rerio were subjected to OECD test No. 215 (fish, juvenile growth test) with salicylic acid concentrations of 0.004; 0.04; 0.4; 4 and 40 mg/L. Specific growth rate (SGR), histological changes, and parameters of oxidative stress were evaluated. SA had no effects on histological changes, SGR, glutathione reductase, and lipid peroxidation. Increased catalytic activity of GPx was found at 0.04 mg/L compared to control, increased catalytic activity of catalase was found at 0.04 and 4 mg/L compared to control, and increased catalytic activity of glutathione-S-transferase was found at 0.004 and 0.04 mg/L compared to control (P < 0.05). Juvenile zebrafish turned out to be relatively insensitive to both environmentally relevant (0.004 mg/L) and higher concentrations of salicylic acid.

Negative Trends in Transport-related Mortality Rates in Broiler Chickens.

The high incidence of deaths during transport for slaughter is associated with poor welfare and represents a considerable loss to the poultry industry. In the period from 2009 to 2014, all shipments of broiler chickens to poultry processing plants were monitored in the Czech Republic and the numbers of chickens transported and those dying as a result of their transport were recorded and analysed. Overall transport-related mortality of broiler chickens transported for slaughter in the Czech Republic was 0.37%. It ranged from 0.31% to 0.72%, the increase approximately corresponding to the increasing transport distance. Statistically highly significant (p<0.001) differences were found when comparing transport-related mortality rates in individual seasons of the year. The greatest mortality (0.55%) was associated with transports carried out in winter months whereas the lowest death losses (0.30%) were found in chickens transported for slaughter in summer months. Our study revealed greater transport-related mortality rates in broiler chickens transported for slaughter in the Czech Republic than expected when considering earlier studies. The most pronounced increases were found in transports for shorter distances and in winter months. However, an increase was found at all transport distances monitored except for distances exceeding 300 km and all seasons except for summer. Furthermore, a general increasing tendency in chicken losses during the monitored period was found. The particularly alarming finding is that the mortality of broiler chickens being transported to processing plants has been showing a long-term increasing tendency over the last two decades. Further research should focus on the identification of specific factors leading to such high and growing mortality rates and developing practical guidelines to improve the welfare of the birds in transit accordingly.

Simultaneous determination of ten anticoagulant rodenticides in tissues by column-switching UHPLC-ESI-MS/MS.

This paper describes the development of a method for the simultaneous determination of ten anticoagulant rodenticides (coumafuryl, warfarin, pindone, coumatetralyl, coumachlor, difenacoum, bromadiolone, brodifacoum, chlorophacinone and flocoumafen) in the liver and kidney based on column-switching liquid chromatography coupled with heated electrospray ionization tandem mass spectrometry. The simple sample preparation includes extraction with methanol. A C18 trapping column was used for online solid-phase extraction before analytical separation with the mobile phase comprising a mixture of 0.1% formic acid in water, methanol and acetonitrile. Chromatographic separation was achieved using a Thermo Hypersil ultra high-performance liquid chromatography (UHPLC) C18 column with the mobile phase consisting of 5 mM ammonium formate buffer (pH = 9) and methanol. The column-switching procedure ensured no matrix effects during electrospray ionization (ESI). Extraction recoveries ranged between 91 and 100% for liver and between 89 and 97% for kidney. The method showed good linearity up to 750 ng g(-1). The limit of detection ranged between 0.001 and 0.022 ng g(-1) for liver and between 0.001 and 0.028 ng g(-1) for kidney. The developed method was successfully used in several animal poisoning cases.

Evaluation of mercury contamination in dogs using hair analysis.

OBJECTIVES: The present work is aimed at assessing the mercury contamination of dogs through the analysis of hair. For the determination of the total mercury in dogs, we chose skin derivatives--hair. The content of total mercury was also measured in the pelleted feed. METHODS: Dogs were divided into two groups. The first group (group A) was fed granular feed containing fish and the second group (group B) granular feed free of fish. A total of 131 hair and granular feed samples were collected. The total mercury in hair and granular feed samples was measured using atomic absorption spectrophotometry on AMA 254. RESULTS: The values of the total mercury content in the hair of dogs had a median value of 0.0375 mg.kg(-1) in group A and of 0.0336 mg.kg(-1) in group B. No statistically significant difference was found between the groups (p>0.05). The median values of the total mercury were 0.0048 mg.kg(-1) in group A and 0.0017 mg.kg(-1) in group B, respectively. A highly statistically significant difference between the groups was found (p<0.01). CONCLUSIONS: No correlation was obtained between the total mercury content in the hair of dogs and granulated feed (rs=0.2069, p>0.05). The reason may be a content of various mercury species in feed samples or a human failure (nonobservance of the prescribed diet).

Haematological and biochemical parameters during the laying period in common pheasant hens housed in enhanced cages.

The development of selected haematological and biochemical parameters during the laying period was monitored in common pheasant hens housed in an enhanced cage system. The cages were enhanced by the addition of two perches and a shelter formed by strips of cloth hanging in the corner of the cage. The results showed significant changes in the haematological and biochemical parameters monitored during egg laying. At the time when laying capacity approached a maximum, a decrease was observed (P < 0.05) in haematocrit, erythrocytes, and haemoglobin values, whereas monocytes, eosinophils, the heterophil/lymphocyte ratio, phosphorus, and calcium exhibited an increase (P < 0.05). At the end of the laying period, an increase (P < 0.05) was recorded in the count of leukocytes, heterophils, lymphocytes and basophils, the heterophil to lymphocyte ratio, and the concentrations of aspartate aminotransferase, cholesterol, phosphorus, and calcium, whereas lower values (P < 0.05) were recorded for haematocrit and plasma total protein in comparison with the values of the indicators at the beginning of the laying period. The results provide new information about dynamic changes in selected haematological and biochemical parameters in clinically healthy common pheasant hens during the laying period.

Differences in Mortality of Pre-Weaned and Post-Weaned Juvenile European Hedgehogs (Erinaceus europaeus) at Wildlife Rehabilitation Centres in the Czech Republic.

Previous research from several European countries has indicated that the European hedgehog (Erinaceus europaeus) is in decline. Wildlife rehabilitation centres contribute toward the protection of debilitated hedgehogs, including the young. Based on data from 27 wildlife rehabilitation centres, the mortality rate and the release rate of juvenile hedgehogs were evaluated depending on whether they were from normally timed litters (admitted from April to September) or from late litters (admitted from October to March). A total of 4388 juvenile European hedgehogs were admitted to wildlife rehabilitation centres in the Czech Republic from 2011 to 2020. The number of post-weaned young from late litters admitted (28%) did not differ from the number of pre-weaned young from late litters (29%). Where the outcome was known, young from late litters had the highest mortality rate (46%) in the year of admission. The release rate was the highest in post-weaned young from normally timed litters (86%). Further research should focus on the definition of optimal care and treatment of the underlying causes for admission of juvenile hedgehogs. The reproductive strategy (the timing of litters) of European hedgehogs under the climatic conditions of the Czech Republic affects the chance of survival of young at wildlife rehabilitation centres and likely also in the wild.

Impact of the Application of Gaseous Ozone on Selected Pathogens Found in Animal Shelters and Other Facilities.

Correctly selecting disinfection procedures is crucial in facilities housing a high number of animals as it directly affects their health. The aim of this study was to verify the virucidal effect of gaseous ozone delivered by commercially available generators under controlled experimental conditions on a selection of viral pathogens (feline coronavirus, canine coronavirus, feline calicivirus, feline parvovirus) commonly found in shelters and other facilities. Two ozone generators with outputs of 3.5 g/h and 20 g/h were used to produce ozone. Virus viability after the application of ozone was evaluated by examining for typical pathogen-specific cytopathic effects on the CRFK (Crandell-Rees Feline Kidney) cell line post-incubation. No cytopathic effect was observed in feline coronavirus after the 2-h application of ozone; in canine coronavirus, the absence of a cytopathic effect was observed after the 4-h application of ozone. The absence of a cytopathic effect in feline calicivirus was observed after the 6-h application of ozone; the viability of feline parvovirus was not impaired even by the 6-h application of ozone. The results of the study confirm lower resistance to the application of gaseous ozone in enveloped viruses.

The Incidence of Liver Damage Found during Postmortem Examination at the Slaughterhouse.

We monitored liver damage in cattle (cows, heifers, fattening bulls, and calves culled from the herd), pigs (sows, finishing pigs, and piglets culled from the farm), sheep (ewes and lambs), goats (does and kids), rabbits, and poultry (end-of-lay hens, broiler chickens, turkeys, domestic ducks, and domestic geese) in the period from 2010 to 2021. All animals (n = 1,425,710,143) reared on Czech farms and slaughtered at slaughterhouses in the Czech Republic were included in the analysis. We determined the total number of damaged livers for individual categories of animals and also analyzed separately the incidence of damage of acute, chronic, parasitic, and other origin. The overall incidence of liver damage was higher in adult animals compared to fattening animals in all species. In cattle and pigs, the incidence was also higher in young animals culled from the herd compared to fattening animals. When comparing adult animals by species, the incidence of liver damage was highest in cows (46.38%), followed by sows (17.51%), ewes (12.97%), and does (4.26%). When comparing fattening animals by species, the incidence was highest in heifers (14.17%) and fattening bulls (7.97 %), followed by finishing pigs (11.26%), lambs (4.73%), and kids (0.59%). When comparing young culled from the herd by species, it was higher in piglets (32.39%) than in calves (17.6 %), and when poultry and rabbits were compared, the incidence was highest in turkeys (3.38%), followed by ducks (2.20%), geese (1.09%), broiler chickens (0.08%), and rabbits (0.04%). The results indicate that fattening animals have a better liver condition than mature animals and that culled young have a worse liver condition than older fattening animals. Chronic lesions represented the dominant proportion of pathological findings. Parasitic lesions occurred, first and foremost, in animals grazed on meadows with likely parasitic invasion, i.e., in ewes (7.51%), lambs (3.51%), and heifers (1.31%), and in animals in which antiparasitic protection is limited in view of the protection of meat from antiparasitic residues, i.e., finishing pigs (3.68%). Parasitic damage to the liver was rarely detected in rabbits and poultry. The results obtained represent a body of knowledge for measures to improve the health and condition of the liver in food animals.

Determination of diurnal rhythm of salivary corticosterone concentration in the domestic rabbit (Oryctolagus cuniculus f. domestica) using a non-invasive method.

There are many advantages associated with the determination of the level of corticosterone in rabbits from saliva, since this is a non-invasive sample collection method that does not affect their welfare and provides a reliable reflection of the state of the animal at a given moment without the results being distorted as they may be, for example, when blood samples are taken. The aim of this study was to determine the diurnal rhythm in the concentration of corticosterone in the saliva of the domestic rabbit. Saliva samples were taken from six domestic rabbits five times during the daytime (at 6:00, 9:00, 12:00, 15:00 and 18:00) over the course of three consecutive days. The levels of corticosterone in the saliva of the individual rabbits displayed a diurnal rhythm during the course of the day, with a significant increase between 12:00 and 15:00 (p < 0.05). No statistically significant difference in the concentrations of corticosterone in the saliva of the individual rabbits was demonstrated. Although the basal value of corticosterone is not known in rabbits and is difficult to determine, the results of our study show the pattern of fluctuations in the concentration of corticosterone in the saliva of rabbits during the daytime.

Diurnal raptors at rescue centres in the Czech Republic: Reasons for admission, outcomes, and length of stay.

Rescue centres play an important role in the protection of raptors living in the wild by caring for injured or debilitated animals and abandoned young with the aim of returning them to the wild. A total of 22,538 raptors were admitted to 34 rescue centres in the Czech Republic in the years 2010-2019, with an increasing trend during the monitored period (rSp = 0.7333, p < 0.05). The most frequent reasons for their admission were other injuries and fractures (26.52%), the admission of young (22.98%), and the admission of raptors injured by electric shock injuries (20.51%). It proved possible to release 42.45% of admitted raptors back into the wild, the majority of which (91.05%) were released using the hard-release method. Foster parents were used in 1% of cases and a replacement nest in 0.2% of cases involving the rearing of young. In spite of all the care provided at rescue centres, a total of 39.97% of raptors admitted either died or had to be euthanized. Among them, most raptors were euthanized or died due to injuries caused by collision with a vehicle, electric shock injuries, and other injuries. This generally occurred shortly after admission (a median of two days). The importance of the work of rescue centres lies not merely in returning injured raptors back into the wild (which proves possible in around half of all cases), but also in obtaining information about the factors endangering raptors in the wild and contributing toward a decline in their populations. The findings provide information about human-wildlife interactions in the Czech Republic and their implications for conservation as well as on the effectiveness of rescue centres to successfully treat and subsequently release raptors back into the wild.

Determination of bisphenol A in commercial cat food marketed in the Czech Republic.

OBJECTIVES: Bisphenol A (BPA) is one of the most widely used synthetic compounds on the planet. It is used in the synthesis of polycarbonate plastics, epoxy resins and other polymer materials. Owing to its excellent chemical and physical properties, it is used to produce food and beverage containers or the linings for metal products. BPA has been mentioned as a possible cause of feline hyperthyroidism. Cat food is considered one of the main sources of BPA intake. The purpose of this study was to evaluate BPA concentration in various types of commercial cat food available in the Czech Republic. METHODS: In total, 172 samples prepared from 86 different types of commercial cat food were assessed. The concentration of BPA was measured using liquid chromatography-tandem mass spectrometry. RESULTS: Measurable concentration of BPA was found in all samples (range 0.065-131 ng/g), with the highest concentration (mean ± SD) of BPA in canned food (24.6 ± 34.8 ng/g). When comparing BPA concentration in food trays (1.58 ± 0.974 ng/g), pouches (0.591 ± 0.592 ng/g) and dry food (1.18 ± 0.518 ng/g), concentrations of BPA in food trays and dry food were significantly higher (P <0.01) compared with pouches. Comparing BPA concentrations in canned food of different manufacturers, statistically significant differences were found as well. CONCLUSIONS AND RELEVANCE: The highest concentrations of BPA were found in cans. Thus, cans represent the highest possibility of exposure to BPA in comparison with other types of commercial feline food.

The morbidity and the spectrum of applied drugs in shelter cats in the Czech Republic.

Maintaining appropriate living conditions and good health of the animals should be one of the main priorities of cat shelters. The aim of this study was to assess the morbidity of shelter cats on the basis of analysis of the shelter health records in terms of the nature and quantity of medicaments and other supportive products administered in two no-kill shelters in the Czech Republic. The subject of the study were the health records of 1,884 cats, which were admitted to the selected shelters from 1.1.2013 to 31.12.2021 and their stay in the shelter was terminated in the monitored period. More than half of all cats whose records were analyzed had at least one health record made during their stay in the shelter. The overall length of stay of cats in the shelter (regardless of the outcome) in which one or more health records were found was significantly longer compared to the length of stay of cats without any health records. The highest number of health records was found in kittens <6 months old. Antibiotics, antiparasitics, and complementary/protective substances were the most used among all administered drugs. Broad-spectrum antibiotics have been administered to cats most often. In terms of classification of antibiotics according to the active substance, the most used antibiotics were penicillins, tetracyclines, and fluoroquinolones. Antibiotics were mostly used to treat diseases related to the upper and lower respiratory tract and their symptoms and gastrointestinal problems. The findings of the study contribute to understanding health problems and approaches to treating the shelter cats.

Reduction of the occurrence of incorrect stunning and the occurrence of reflexes and reactions in cattle after pneumatically powered captive-bolt stunning in comparison with cartridge-fired captive-bolt stunning.

In this study, the occurrence of repeat stunning, deviations in placement and direction of stun shots, and the occurrence of reflexes/reactions following stunning with a pneumatically powered captive-bolt stunner in comparison with a cartridge-fired captive-bolt stunner in cattle were assessed. Repeat stunning and deviations in placement and direction of the stun shots were less frequent (p < 0.01) with the use of a pneumatic stunner in all cattle. The rate of repeat stunning and deviation from the ideal place and direction of stun shots were lower (5.81%, 24.75%, and 9.60% of all animals, respectively) when a pneumatic stunner was used in comparison with cartridge-fired captive bolt (18.32%, 34.03%, and 47.38% of all animals, respectively). The stunning quality was assessed on the basis of evaluation of signs observed following the stun shot. A pneumatic stunning resulted in a significant reduction of occurrence of signs particularly in bulls in comparison to cows, for which a cartridge-fired stunner already provides an adequate stunning. Our study provides evidence that pneumatic stunning has the potential to be a more effective method than cartridge-fired stunning from the viewpoint of the welfare of slaughtered animals. Pneumatic stunning ensures a high quality of stunning in both bulls and cows.

Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters.

The aim of this study was the direct detection of feline coronavirus by real-time PCR and by three different rapid immunochromatographic (RIM) tests detecting antigens in faecal samples of shelter cats. Based on sensitivity and specificity calculated for each of the RIM tests, the utility of RIM tests was compared. Seventy faecal samples originating from shelter cats housed in quarantine were examined. Out of 70 samples analyzed by real-time PCR, 44 (62.9%) were positive. Significantly more cats (p < 0.05) tested positive than negative. Neither age nor sex of the cats played a significant role (p > 0.05) in the shedding status of the virus. The sensitivity of the RIM tests was found to be at low (<35%; RIM tests A and C) to satisfactory level (>50%, RIM test B). The number of virus particles determined by real-time RT-PCR analysis did not significantly correlate with the results detected by any of the RIM tests (p > 0.05). The results of this study indicate that the use of rapid antigen RIM tests in routine screening of FCoV shedding status in shelter cats is limited due to the occurrence of a high number of false negative results.

Changes in Health Indicators of Welfare in Group-Housed Shelter Cats.

The aim of this study was to detect changes in health-oriented welfare indicators of shelter cats housed in a shelter by means of long-term monitoring of health indicators of welfare in a population of group-housed cats in a private, no-kill shelter in the Czech Republic. The cat population housed in a large group was monitored for 1 year. The data recording took place at 2-week intervals. A total of 220 cats were evaluated using a protocol containing eight health-oriented welfare indicators: body condition, third eye visibility, eye discharge, eye irritation, nose discharge, the presence of pathologically induced respiratory sounds, coat condition, and lameness and abnormal posture. The assessment was performed based on the observation by two trained evaluators. The cats' condition was rated on a 5-point scale for each indicator, where the optimal condition was represented by the score of 1 and a severe deterioration by the score of 5 for the given indicator, except for the body condition indicator, where the optimal condition was represented by the score of 3. A deterioration in the score in at least one of the indicators during the stay in the shelter was recorded in 52 (41.6%) of 125 cats that were assessed at least twice. The effect of the LOS, sex and age on the scores for each health indicator was examined via a linear mixed model analysis, as this method allows for handling of dependencies in the data of repeated measurements. The effect of predictors on the third eye visibility scores was not found. The age of cats predicted the coat condition and body condition scores. The LOS predicted scores of the abnormal posture and lameness indicator and scores of a composite index composed of indicators related to upper respiratory tract disease. The results suggest that despite the fact that some improvement of health was documented during the cats' stay in the shelter, there were a non-negligible number of animals experiencing a permanent or long-term deterioration in health. Efforts to minimize the undesirable factors contributing to the deterioration of well-being of cats during their stay in a shelter should be made.

Juvenile European hedgehogs (Erinaceus europaeus) at rescue centers and their release rate depending on their weight on admission.

This study aimed to assess the numbers of juvenile European hedgehogs admitted to rescue centers in the Czech Republic from the viewpoint of their weight on admission, the reason for their admission, and the success rate of their release back into the wild. The results of our study show varying levels of success in the rearing of hedgehogs admitted at different ages (weights) and a varying period required for their rehabilitation. The greatest chance of release was seen in hedgehogs with a weight on the admission of 500-599 g (64.22% released) and 400-499 g (63.31% released). In contrast, the smallest number of young hedgehogs successfully rehabilitated and released was seen in hoglets weighing 200-299 g (35.24% released) on admission, which corresponds to the weight of hedgehogs at the time of weaning. Time spent at a rescue center may pose an undesirable threat to the lives of animals in some categories. Hedgehogs weighing up to 99 g on admission spent the longest period time at rescue centers (a median of 48 days), while hedgehogs weighing 500-599 g on admission spent the shortest time (a median of 7 days). The majority of hedgehogs in the lowest weight categories were admitted due to their inability to survive on their own. A large percentage of hedgehogs of greater weight, in contrast, were juvenile hedgehogs brought to rescue centers needlessly. The percentage of released animals did not exceed 65%, however, even for entirely independent categories of older juveniles. From this perspective, the fact that hedgehogs are often brought to rescue centers in the belief that they are not self-sufficient young, though they are actually juvenile or even adult individuals that do not require human care, can be considered a significant finding.