Ecophysiological responses of Liolaemus arambarensis juveniles to experimental temperature variations.

Climate change increasingly influences the loss of biodiversity, especially in ectothermic organisms, which depend on environmental temperatures to obtain heat and regulate their life cycle. Studies that aim to understand the impact of temperature variation are important to better understand the possible impacts generated on the homeostasis of ectothermic organisms. Our objective was to characterize the responses of juvenile Liolaemus arambarensis lizards to abrupt changes in temperature, quantifying markers of body condition, intermediary and hormonal metabolism and oxidative balance. We collected 45 juvenile individuals of L. arambarensis (winter: 20 and summer: 25) in Barra do Ribeiro, Brazil. We transported the animals to the laboratory, where they were acclimatized for five days at a temperature of 20 °C, then divided and exposed to temperatures of 10 °C, 20 °C, 30 °C and 40 °C for 24 h. After exposure, the animals were euthanized and the brain, caudal muscle, thigh, and liver tissues were extracted for quantification of biomarkers of metabolism (glycogen and total proteins) and oxidative balance (acetylcholinesterase, superoxide dismutase, catalase, glutathione-S-transferase and lipoperoxidation) and plasma for corticosterone quantification. The results show that L. arambarensis is susceptible to sudden temperature variations, where higher temperatures caused greater activity of antioxidant enzymes, increased lipoperoxidation and higher plasma levels of corticosterone in animals eliminated in winter. The present study demonstrated that abrupt changes in temperature could significantly modify the homeostatic mechanisms of animals, which could lead to oxidative stress and a potential trade-off between survival and growth/reproduction. In this context, the organism mobilizes energy resources for survival, with possible damage to growth and reproduction. Demonstrate that a change in temperature can be a potential factor in extinction for a species given the profile of global climate change.

Phylogeny of Riama (Squamata: Gymnophthalmidae), impact of phenotypic evidence on molecular datasets, and the origin of the Sierra Nevada de Santa Marta endemic fauna.

Riama is the most speciose genus of the Neotropical lizard family Gymnophthalmidae. Its more than 30 montane species occur throughout the northern Andes, the Cordillera de la Costa (CC) in Venezuela, and Trinidad. We present the most comprehensive phylogenetic analysis of Riama to date based on a total evidence (TE) approach and direct optimization of molecular and morphological evidence. Analyses use DNA sequences from four loci and 35 phenotypic characters. The dataset consists of 55 ingroup terminals representing 25 of the 30 currently recognized species of Riama plus five undescribed taxa, including an endemic species from the Sierra Nevada de Santa Marta (SNSM) in Colombia, and 66 outgroup terminals of 47 species. Analysis results in a well-supported hypothesis in which Riama is polyphyletic, with its species falling into three clades. The Tepuian Anadia mcdiarmidi nests within one clade of Riama, and the recently resurrected Pantodactylus nests within Cercosaura. Accordingly, we propose a monophyletic taxonomy that reflects historical relationships. Analysis of character evolution indicates that the presence/absence of prefrontals-a cornerstone of the early genus-level taxonomy of cercosaurines-is optimally explained as having been plesiomorphically present in the most recent common ancestor of Cercosaurinae and lost in that of the immediately less inclusive clade. Multiple independent reversals to present and subsequent returns to absent occur within this clade. To evaluate the impact of phenotypic evidence on our results, we compare our TE results with results obtained from analyses using only molecular data. Although phenotypic evidence comprises only 1.2% of the TE matrix, its inclusion alters both the topology and support values of the clades that do not differ. Finally, current phylogenetic evidence reveals a SNSM-CC-Trinidad-tepuis biogeographical link. We hypothesize that an ancient connection facilitated the exchange of species between the SNSM and the CC.

Envenomation by opisthoglyphous snake Thamnodynastes hypoconia (Cope, 1860) (Dipsadinae: Tachymenini) in southern Brazil.

We report here a case of human envenoming by Thamnodynastes hypoconia, a common and abundant non-front-fanged snake belonging to the subfamily Dipsadinae. The case was registered in the municipality of Tapes, Rio Grande do Sul state, Brazil, in a 27-year-old female. The snakebite was on the wrist of the left arm while handling the snake in a field outing. No pain sensation was noted during the bite, and after 20 minutes edema developed along the hand and forearm with a slight sensation of numbness and mild pain when moving the fingers. After 15 hours, the victim began to develop erythema, paraesthesia, and a sensation of warmth at the bite site. After 30 hours, ecchymosis occurred on the fingers and forearms, and the edema began to decrease. After 70 hours from the time of the bite, ecchymosis along with pruritus and mild pain were still evident. The patient was treated with prescribed medications, and after 7 days no further symptoms were observed. This is the first reported case of envenoming by T. hypoconia.

What do stable isotopes tell us about the trophic ecology of Thamnodynastes hypoconia (Serpentes: Dipsadidae) in southern Brazil?

Snakes are a useful model for ecological studies because they are gape-limited predators that may undergo ontogenetic changes in diet. We analyzed carbon and nitrogen stable isotope ratios to estimate percent contributions of different prey to snake biomass, trophic positions and isotopic niche width of juveniles and adults of the snake Thamnodynastes hypoconia. We also estimated the isotopic niche overlap between the two age categories. During eight intervals over a two-year period, we collected samples of whole blood and scales at a site in southern Brazil. Isotopic ratios of carbon and nitrogen did not differ between juveniles and adults for either tissue type, nor did mean trophic positions of juveniles and adults differ. The percent contribution of prey categories to snake biomass differed to a limited extent between the two years, with Hylidae being the most important anuran group assimilated during the first year and Leptodactylidae during the second year, for both ages. The isotopic niche occupied by adult snakes was slightly larger than that of juveniles when the analysis was based on data from whole blood samples, as expected because snakes are gape-limited. We found a reverse pattern when the analysis was based on scales, which may indicate that adult snakes have a smaller niche over the long term as they become selective foragers in certain prey. Isotopic overlap between juveniles and adults occurred during the two years, but it was bigger during the second year. We infer that, despite differences in gape size, juvenile and adult snakes in the study area exploit similar prey, with the degree of trophic similarity varying interannually.

Are lizards sensitive to anomalous seasonal temperatures? Long-term thermobiological variability in a subtropical species.

Alterations in thermal niches have been widely associated with the Anthropocene erosion of reptiles' diversity. They entail potential physiological constraints for organisms' performance, which can lead to activity restrictions and impact fitness and demography. Reptiles are ectotherms which rely on seasonal periodicity to maximize the performance of biological functions. Despite it, the ecological implications of shifts in local temperatures are barely explored at the seasonal scale. This study aims to assess how changes in air temperature and substrate temperature affect the activity, body temperature (Tb) and thermoregulation patterns of the sand lizard, Liolaemus arambarensis (an endangered, microendemic species from southern Brazil), throughout a four-year period. Field surveys were conducted monthly on a restricted population in a sand-dune habitat. The annual fluctuations of the seasonal temperatures led to significant changes in the activity and Tb of L. arambarensis and shaped thermoregulation trends, suggesting biological plasticity as a key factor in the face of such variability. Lizards tended to maintain seasonal Tb in mild and harsh seasons through increased warming/cooling efforts. Anomalous winter conditions seemed especially critical for individual performance due to their apparent high impact favouring/constraining activity. Activity and thermoregulation were inhibited in frigid winters, probably due to a vulnerable physiology to intense cold spells determined by higher preferred body temperatures than Tb. Our results warn of a complex sensitivity in lizards to anomalous seasonal temperatures, which are potentially enhanced by climate change. The current work highlights the importance of multiannual biomonitoring to disentangle long-term responses in the thermal biology of reptiles and, thereby, to integrate conservation needs in the scope of global change.

So far away, yet so close: strong genetic structure in Homonota uruguayensis (Squamata, Phyllodactylidae), a species with restricted geographic distribution in the Brazilian and Uruguayan Pampas.

The Pampas is a biologically rich South American biome, but is poorly represented in phylogeographic studies. While the Pleistocene glacial cycles may have affected the evolutionary history of species distributed in forested biomes, little is known about their effects on the habitats that remained stable through glacial cycles. The South American Pampas have been covered by grasslands during both glacial and interglacial periods and therefore represent an interesting system to test whether the genetic structure in such environments is less pronounced. In this study, we sampled Pampean populations of Homonota uruguayensis from Southern Brazil and Uruguay to assess the tempo and mode of population divergence, using both morphological measurements and molecular markers. Our results indicate that, in spite of its narrow geographic distribution, populations of H. uruguayensis show high levels of genetic structure. We found four major well-supported mtDNA clades with strong geographic associations. Estimates of their divergence times fell between 3.16 and 1.82 million years before the present. Populations from the central portion of the species distribution, on the border between Uruguay and Brazil, have high genetic diversity and may have undergone a population expansion approximately 250,000 years before the present. The high degree of genetic structure is reflected in the analyses of morphological characters, and most individuals could be correctly assigned to their parental population based on morphology alone. Finally, we discuss the biogeographic and conservation implications of these findings.

Playing dead to stay alive: death-feigning in Liolaemus occipitalis (Squamata: Liolaemidae) / Simulando a morte para manter-se vivo: tanatose em Liolaemus occipitalis (Squamata: Liolaemidae)

Predation is the major selective force that drives the development of a series of defense mechanisms by the species. These mechanisms are efficient in that it limits the ability of predators to detect, recognize and subdue their prey. In lizards for example, the tail loss and locomotor escape are the most common defensive behavior reported on the literature. Additionally, in response to external stimuli, some lizards exhibit a peculiar defensive behavior: death-feigning or thanatosis. Here we describe the first record of death-feigning in a population of Liolaemus occipitalis in a coastal sand dune habitat in Southernmost Brazil. A total of 86 individuals were tested. During handling 75.6 percent of lizards feigned death. The duration of this behavior was longer when the observer remained closer to the lizards, suggesting the ability of L. occipitalis to evaluate the predation risk.

A pressão exercida pela predação é a principal força seletiva que direciona o estabelecimento de uma série de mecanismos de defesa por parte das espécies. Esses mecanismos são eficientes na medida em que limitam a capacidade dos predadores em detectar, reconhecer ou subjugar suas presas. Em lagartos, por exemplo, os comportamentos defensivos mais frequentemente citados na literatura são a autotomia caudal e a fuga. Além disso, em resposta a estímulos externos, alguns lagartos podem exibir um comportamento peculiar: fingem-se de mortos (tanatose). Neste estudo descrevemos o primeiro registro de tanatose em uma população de Liolaemus occipitalis em área de dunas costeiras no extremo Sul do Brasil. Um total de 86 indivíduos foram testados. Durante o manuseio, 75,6 por cento dos lagartos exibiram o comportamento de tanatose. A duração deste comportamento foi maior quando o observador permanecia a distâncias mais curtas dos lagartos, sugerindo uma capacidade de L. occipitalis em avaliar o risco de predação.