Root canal conicity determination of primary second molars using nanotomography: An in vitro study.

BACKGROUND: The conicity of the root canals of primary teeth is an important measure for endodontic therapies. However, determining this conicity depends on the methods employed, which requires further investigation. AIM: The aim of this study was to determine the conicity of the root canals of the upper and lower primary second molars using nanotomography (nCT). DESIGN: An in vitro study was performed using nine primary second molars, both upper and lower, subjected to nCT. Comparisons between the diameters of root canals were performed between the thirds (cervical-D0, middle-D5, and apical-D7). The conicity (%) was determined for each root canal from cervical to apical. Data were statistically analyzed with a significance level of 5%. RESULTS: The conicity ranged from 2% to 8% for the upper primary second molars. Significant differences in root canal diameter between the thirds (D0, D5, and D7 points) were observed in the mesio- and distobuccal roots (p < .05), but not in the palatal roots (p > .05). For the lower primary second molars, the conicity ranged from 2% to 17%, as well as significant differences in root canal diameter between the thirds (D0, D5, and D7 points) were observed in all roots (distal, mesiobuccal, and mesiolingual; p < .05). CONCLUSION: The conicity of the upper primary second molars was different from that of the lower ones, which showed a greater variability.

Changes in the Oral Health-Related Quality of Life in Infants With Cleft Lip and/or Palate Before and After Surgical Treatment.

OBJECTIVE: To assess oral health-related quality of life (OHRQoL) changes before and after the primary surgical treatment in infants with cleft lip and/or palate (CL/P). DESIGN: Quasi-experimental study. METHODS: A total of 106 infants with CL/P younger than 2 years undergoing primary surgical treatment in the Plastic Surgery Service of the Instituto Nacional de Salud del Niño in Peru. The parent/caregiver answered a questionnaire about OHRQoL named the Peruvian version of the Early Childhood Oral Health Impact Scale (P-ECOHIS) in the pretreatment (baseline) and follow-up post-treatment. The total score of P-ECOHIS and their 2 sections (child impact and family impact) in the baseline and each follow-up period post-treatment scores were assessed. As well as, the type of the CL/P on OHRQoL, standardized effect sizes (ES) based on mean total change scores (difference between baseline and 12th month) were analyzed. RESULTS: Improvements in infant's OHRQoL after treatment were reflected in each follow-up period P-ECOHIS score compared to the baseline score. The total P-ECOHIS scores decreased significantly from 28.07 (baseline) to 7.7 (12th month; P < .0001), as did the individual domain scores (P < .0001). There were significant differences in the baseline and follow-up post-treatment scores of infants who reported improvement of the OHRQoL (P < .0001). The ES was large (3.79). The cleft lip had an improvement in the OHRQoL at 12th month post-treatment (P < .0001). CONCLUSIONS: Primary surgical post-treatment resulted in significant improvement of the infant's OHRQoL with CL/P.

Root canal conicity estimation of primary maxillary central and lateral incisors-A study by Nano-CT.

AIM: The objective of the study was to estimate the conicity of the root canals of maxillary central and lateral incisors by computed nanotomography (Nano-CT). DESIGN: This in vitro study included nine extracted primary maxillary central incisors and 12 maxillary lateral incisors, which were subjected to Nano-CT analysis. The resulting images of each tooth were reconstructed using the OnDemand3D software, and root canal area, volume, and taper analysis were performed using the free FreeCAD 0.18 software for the 3D computer-aided design (CAD) model. Data were statistically analysed using the Stata v14.0 software, adopting a significance level of 5%. RESULTS: The results presented the mean value of the diameter and area of the root canal of primary central and lateral incisors. In addition, the taper values for both dental groups between points D0-D5, D5-D7, and D7-D10 were determined. Considering the diameters obtained over the entire length of the root, with a length of 12 mm, a conical model was constructed. CONCLUSION: Detailed knowledge of root morphology of maxillary central and lateral incisors of primary dentition by means of Nano-CT is important to achieve faster, more accurate, and efficient endodontic treatments.

Factors associated with indication of prophylactic extraction of the lower third molar in orthodontic practice.

Background: To date, there is no consensus on the factors that influence on indication for prophylactic extraction of the third molar, however it is a common indication in orthodontics. Aim: To determine the factors associated with indication of prophylactic extraction of the lower third molar in orthodontic practice. Material and Methods: This cross-sectional analytical study used an online survey to obtain responses from 100 professionals with clinical practice in orthodontics. The Survey Monkey software was used to enter a valid and reliable questionnaire of 11 questions to obtain demographic and clinical information of the professional, as well as some patient conditions that could be considered in a possible indication for prophylactic extraction. The questionnaire was sent through social networks and instant messaging applications. Chi Square test was used to evaluate associated factors and binomial logistic regression to identify risk or protective factors. Results: Factors significantly associated with indication of prophylactic extraction of the lower third molar were experience in orthodontics (p-value = 0.060; OR=0.325), characteristics of impaction (p-value = 0.012; OR=3.689), prevention of pericoronitis (p-value = 0.014; OR=3.769) and help stability of treatment results (p-value = 0.002; OR=6.074). Conclusions: The risk factors to indication for prophylactic extraction of the lower third molar were impaction of the third molar, prevention of pericoronitis and helping the stability of the results after treatment. Furthermore, experience in orthodontics was identified as a protective factor for this indication. Key words:Orthodontics, risk factors, third molar, tooth extraction.

Multivariate analysis of the effect of staining beverages on the optical properties of two provisional restorative materials.

Background: To evaluate the effect of staining beverages on the color stability, translucency and gloss of two provisional restorative materials. Material and Methods: Sixty discs (8 mm x 2 mm) were manufactured for Duralay and Protemp 4. The discs were randomly divided according to the beverages: tea, coffee, wine, Coca-Cola and "Chicha morada" (n=12). The discs were polished and initial recordings of color and translucency were made with a spectrophotometer, and the gloss was measured with a glossmeter. The discs were immersed for 5 days in each of the beverages at 37◦C, and the color, translucency and gloss were recorded again. The differences between the initial and final records were calculated to obtain the values of ΔE, ΔTP, ΔGU. For the analysis, the two-way MANOVA model was chosen, and the significance level was set at 5%. Results: A significant interaction was observed between the type of material and the staining beverages on the changes observed in the values of ΔE, ΔTP, ΔGU (p<0.05). ΔE values for Duralay and Protemp 4 were affected by coffee (7.48±1.53) and wine (11.02±1.07), respectively. The greatest change in ΔTP for Duralay were generated by tea (-1.79±0.62), and coffee (-5.65±0.66) for Protemp 4. Gloss was affected mainly by coffee for both materials (Duralay = -6.44±1.17 , Protemp 4 = -8.28±1.09). Conclusions: The type of material and the pigment drinks act together to influence changes in color, translucency and gloss. The methacrylate-based resin was more stable than the bis-acrylic resin to changes in color, translucency and gloss. Key words:Color, translucency, gloss, staining solutions, interim restorations.

Estimation of root canal conicity of deciduous canines evaluated by nano-CT.

PURPOSE: To estimate the taper of root canals of deciduous maxillary and mandibular canines by nano computed tomography (nano-CT). METHODS: This in vitro study involved CT scan analysis of nine maxillary and five mandibular primary canines. The images of each tooth were reconstructed using OnDemand3D software. Thereon, diameter and taper analyses were performed on the free FreeCAD 0.18 software for the three-dimensional (3D) computer-aided design model. Statistical analysis was conducted using Stata v14.0 software, adopting a significance level of 5%. RESULTS: 3D image reconstruction was performed, considering the diameters obtained along the entire length of the tooth root, and the conical model was built with a height of 10 mm. The diameters of the maxillary canine at points D0 (0 mm), D5 (5 mm), D7 (7 mm), and D10 (10 mm) were 1.62, 1.07, 0.78, and 0.49 mm, respectively, with a significant difference between the four points (p = 0.0001). Regarding maxillary canine root taper values in the cervical, middle, and apical regions, the values were 12%, 14%, and 10%, respectively. For mandibular canines, the mean diameter values obtained at points D0, D5, D7, and D10 were 1.51, 0.83, 0.64, and 0.45 mm, respectively, with significant differences among the four points (p = 0.005). The inferior canine root tapers in the cervical, middle, and apical regions were 14%, 10%, and 6%, respectively. CONCLUSION: The detailed knowledge of the root morphology of maxillary and mandibular deciduous canines, as it has been shown in vitro using nano-CT, is critical to achieve accurate and efficient endodontic treatments.

Bibliometric analysis of fourth industrial revolution applied to heritage studies based on web of science and scopus databases from 2016 to 2021.

Using past material and spiritual remains, cultural heritage examines communities' identity formation across time. Cultural heritage requires public and private institutions to care about its restoration, maintenance, conservation, and promotion. Through a bibliometric perspective, this study has analyzed, quantified, and mapped the scientific production of the fourth industrial revolution applied to heritage studies from 2016 to 2021 in the Scopus and Web of Science databases. Biblioshiny software from RStudio was employed to categorize and evaluate the contribution of authors, countries, institutions, and journals. In addition, VOSviewer was used to visualize their collaboration networks. As a main result, we found that augmented reality and remote sensing represent the research hotspot concerning heritage studies. Those techniques have become common in archaeology, as well as museums, leading to an increase in their activity. Perhaps, more recent tools, such as machine learning and deep learning, will provide future pathways in cultural heritage from data collected in social networks. This bibliometric analysis, therefore, provides an updated perspective of the implementations of technologies from industry 4.0 in heritage science as a possible guideline for future worldwide research.

Correlation between cervical vertebrae maturation and midpalatal suture fusion in patients aged between 10 and 20 years: A cross-sectional and 3D study.

OBJECTIVE: This study aimed to correlate the cervical vertebrae maturation (CVM) stages with the bone fusion stages of the midpalatal suture (MPS). MATERIALS AND METHODS: The sample consisted of 351 cone-beam computed tomographies (CBCTs) of patients divided into two groups: 10-15 years old and 16-20 years old. Both CVM and MPS stages were correlated using Spearman's correlation analysis. To assess the diagnostic performance of the CVM stages in identifying the bone fusion stages of the MPS, the likelihood ratio (LHR) test was applied. RESULTS: In the whole sample, a low positive correlation was found between CVM and MPS bone fusion level (P<0.001; Rho=0.395). In the younger age group, the correlation was moderate in the male (P<0.001; Rho=0.616) and low in the female (P<0.001; Rho=0.394) patients. In the 16-20 years age group, there was no correlation between CVM and MPS fusion. A moderate increase in the probability of determining CS1 for stage A (38%) and CS2 for stage B (42.2%) of MPS ossification was found among the 10-15 year old female patients. CONCLUSION: A low and moderate positive correlation was found between CVM and MPS level of bone fusion in female and male patients respectively, but the diagnostic performance of the CVM stages in the identification of MPS bone fusion stages did not have a high correlation. Therefore, the CVM stages indicated no conclusive probability of determining the MPS bone fusion stage.

Validity and Reliability of questionnaires measuring attitudes to oral health: A review of the literature.

Background: The instruments used to assess attitudes to oral health must be validated in order for their results to be reliable and comparable with other variables. The aim of this review was to analyze the ability of self-administered questionnaires to validly and reliably measure attitudes to oral health. Material and Methods: A bibliographic review was carried out using the following databases, Medline (PubMed), SCOPUS and Web of Science from the year 2016 to 2021, using the keywords: (questionnaire* OR survey*) AND (attitude* OR behav*) AND ("oral health" OR "dental care") AND (validity OR reliability). Results: A total of 234 original articles were found in the databases, only 22 met the selection criteria, of which 13 were aimed at patients and nine at health professionals. Evidence of validity and reliability was determined using "COnsensus-based Standards for the selection of health status Measurement INstruments" (COSMIN). Conclusions: Most of the articles partially meet the validity and reliability criteria. Key words:Questionnaire, attitude, behavior, oral health, validity and reliability.

Association between developmental defects of enamel and early childhood caries in children under 6 years old: A systematic review and meta-analysis.

Early childhood caries (ECC) are an oral health problem worldwide in children under 6 years of age. This disease of rapid development has a multifactorial etiology, and one of the possible risk factors is developmental defects of enamel (DDE), such as hypoplasia and opacities. The aim of this systematic review was to evaluate the association between DDE and ECC in children under 6 years of age. An electronic search was conducted until March 2022 using Medline (PubMed), Scopus, Science-Direct, LILACS, Web of Science, Cochrane Library, EBSCO-Host, EMBASE, and Google Scholar and complemented with a manual search, with no restrictions on language or date of publication. Longitudinal studies of children under 6 years of age with primary dentition were included. A total of 1158 studies were found, of which 651 records were reviewed by title and abstract, and 24 articles were selected for full-text evaluation. Finally, nine studies that met the selection criteria were included in the qualitative synthesis. Study quality and certainty were assessed using the Newcastle-Ottawa scale and the Grading of Recommendations Assessment, Development, and Evaluation (GRADE) tool. Three cohort studies of good quality were included in the meta-analysis. A risk associated with DDE (RR = 1.94; 95% CI: 1.52-2.49) and a risk associated with enamel hypoplasia (RR = 5.45; 95% CI: 1.84-16.14) were found. The results for diffuse opacity (RR = 1.21; 95% CI: 0.18-8.15) and demarcated opacity (RR = 1.26; 95% CI: 0.43-3.65) were not significant. GRADE analysis presented low and very low certainty of evidence. It was concluded that there is an association between DDE and ECC. However, the results should be interpreted with caution because of the limitations of the study. The protocol for this study has been registered in PROSPERO under identification number CRD42021238919.

Evaluation of the suture ossification level according to age and sex in children, adolescents, and adults. A cross-sectional and observational 3D study.

OBJECTIVE: To determine the ossification level of the midpalatal suture (MPS) in children, adolescents and adults from a Peruvian sample; according to the method proposed by Angelieri et al. with cone-beam computed tomographies (CBCTs). MATERIAL AND METHODS: The sample consisted of 315 CBCTs of 168 females and 147 males. The total sample was divided into 3 groups according to age and sex: children (n=77), adolescents (n=113) and adults (n=125). The images were mainly assessed in the axial plane using the Real Scan 2.0 software. The different periods of ossification of the MPS at the level of the vertical half of the palate was defined according to the five stages of Angieleri method (from A to E). The Student t-Test, Chi-square test, Kruskal-Wallis test and Spearman's Rho test were applied. RESULTS: Chi2 test results showed that the stages of MPS ossification depended on the age of the patient by age ranges (P<0.005). Multiple comparison tests affirmed that male and female subjects in the children group had fewer ossification stages than the adolescent and adult groups (P<0.001). Meanwhile, there were no statistically significant differences between the two older age groups. Finally, there was a moderate positive correlation between the stages of ossification of the MPS and the age in the male group (Rho=0.511). CONCLUSIONS: The onset of MPS ossification was significantly related to subjects up to 12 years of age and more frequently in stages B and C in both sexes. There was no difference in MPS ossification in adolescents and adult subjects.

Quality assessment of clinical practice guidelines for the management of paediatric dental emergencies applicable to the COVID-19 pandemic, using the AGREE II instrument. A systematic review.

OBJECTIVE: To assess the quality of Clinical Practice Guidelines (CPG) related to the management of paediatric dental emergencies applicable to the COVID-19 pandemic, through the use of the measuring instrument AGREE II (Appraisal of Guidelines for Research and Evaluation in Europe). SOURCES AND DATA COLLECTION: A rigurous online search of CPG was accomplished among the main CPG compilers: National Institute for Health and Care Excellence (NICE), National Guideline Clearinghouse, Agency for Healthcare, Research and Quality (AHRQ), Andalusian Health Technology Assessment Department (AETSA), American Academy of Family Physicians, Tripdatabase. Furthermore, because of the need to identify CPG that meet the inclusion criteria, a manual search, among the main national and international dental organizations as well as recognized web sites, was also accomplished. SELECTION OF RESEARCH STUDIES: All of the guides focused on paediatric dental emergencies, available in the database and "gray" literature, and published between 2000 and 2020 (applicable to COVID-19 pandemic) were included without any language restrictions. The CPG that did not contain the full paper or were addressed to adults or children with special needs, were excluded from the selection. The evaluation of the CPG, independently included, were achieved by four (04) experts by using AGREE II. RESULTS: Five (05) out of twenty-three (23) selected CPG, were classified as "acceptable" according to AGREE II. These five guides were evaluated to determine their "Recommendation degree". Only one (01) CPG "Guía Clínica AUGE de Urgencias Odontológicas Ambulatorias-Chile, 2011" reached a score of 75%, the highest among the other guides (5 domains with a score ≥ 60%, including the domain III "Rigour of Development") to be considered as a "highly recommended" CPG. CONCLUSIONS: According to the quality assessment and recommendation degrees criteria from AGREE II, high, middle and low quality CPG were identified. Only one CPG reached a score of 75%, to be classified as "highly recommended". Therefore, it is suggested that the existing CPG updates and future CPG use the available tools and methodologies during their elaboration, in order to guarantee their quality. CLINICAL SIGNIFICANCE: High quality CPG for the management of dental emergencies are designed to support dental health professionals in decision-making to adopt specific dental procedures in the current COVID-19 pandemic. As a matter of fact, these CPG might contribute to reduce the risk of transmission, in case of fresh outbreak of the illness. Likewise, they might help to determine which cases warrant medical attention in centres with special facilities for COVID-19.

Involvement of the maxillary sinus ostium (MSO) in the edematous processes after sinus floor augmentation: a cone-beam computed tomographic study.

BACKGROUND: After sinus floor augmentation, a thickening of the sinus mucosa has been described. The aim of the present study was to evaluate the involvement of the maxillary sinus ostium in the edematous processes after a sinus floor augmentation procedure. METHODS: Seventy-two cone-beam computerized tomographies (CBSTs) were taken before sinus floor augmentation and after 1 week and 9 months from surgery and analyses. Sinus mucosa thickness and area, ostium diameter and patency, and extension of the post-surgical transient mucosal thickening in relation to the ostium were evaluated on the CBCTs for all three periods. The term "virtual" when referring to sinus mucosa thickness and area was introduced because of the edema and bleeding that both contributed to a transient thickening and additional elevation of the sinus mucosa. RESULTS: The mean virtual thickness of the sinus mucosa was 2.7 ± 4.0 mm, 7.7 ± 7.1 mm, 1.7 ± 2.0 mm before surgery, and after 1 week and 9 months. The virtual mucosa area was 37.2 ± 52.5 mm2, 184.5 ± 153.8 mm2, and 34.0 ± 50.7 mm2. The ostium diameter at the three periods evaluated was 1.8 ± 0.5 mm, 1.1 ± 0.6 mm, 1.5 ± 0.8 mm, respectively. Three infundibula (4.2%) were found out of patency before surgery while this number increased to 14 (19.4%) after 1 week. Nine months after surgery, only one infundibulum (1.4%) was out of patency, however, without presenting signs of sinus pathologies. The extension of the mucosal edema on the palatal sinus was reduced after 9 months of healing. CONCLUSIONS: One week after sinus floor augmentation, the maxillary sinus mucosa increased in dimensions and in several cases involved the ostium, reducing its diameter and producing a transient loss of patency. After 9 months of healing, the initial conditions were recovered.

Deproteinization of primary enamel with sodium hypochlorite before phosphoric acid etching.

The aim of this study was to evaluate the deproteinization of primary enamel by analyzing etching pattern types, with and without the application of 5% NaOCl before acid etching with 37% H3PO4. Fifteen extracted human primary molars were randomly selected for the present in vitro study; 1mm x 1mm blocks were prepared and divided into two groups (n = 21). These groups were treated as follows: Group A- Acid Etching with 37% H3PO4 gel for 15 s; Group B- 5% NaOCl for 60 s + Acid Etching with 37% H3POfor 15 s. The specimens were prepared for scanning electron microscopy analysis. The images were evaluated for quality types I and II etching of the enamel surface using ImageJ software. Datasets were checked for normality by Kolgomorv-Smirnov test and the nonparametric unpaired Mann-Whitney test was applied. The mean surface area of type I and II etching pattern values was 1922.314 µm2for Group A and 3840.473 µm2Group B. We conclude that deproteinization with 5% NaOCl prior to acid etching can be used to increase the area of adhesion and the quality of the etching pattern.

El objetivo del estudio fue evaluar la desproteinización del esmalte primario a través de los tipos de patrones de grabado, con y sin NaOCl 5% utilizado antes del grabado ácido con H3PO4 37%. Quince dientes primarios humanos extraídos se seleccionaron al azar para el presente estudio in vitro, se prepararon bloques de 1mm x 1 mm y se dividieron en dos grupos (n = 21). Estos grupos se trataron de la siguiente manera: Grupo A: Grabado ácido con H3PO4 37% en gel durante 15 segundos; Grupo B: NaOCl 5% durante 60 segundos + Grabado ácido con H3PO4 37% durante 15 segundos. Las muestras se prepararon para el análisis de microscopía electrónica de barrido. Las imágenes obtenidas se evaluaron principalmente por la calidad de los grabados tipo I y II de la superficie del esmalte primario, utilizando el software Image J. Los datos se analizaron en cuanto a su normalidad mediante la prueba de Kolgomorv-Smirnov, se utilizó pruebas no paramétricas: Prueba de Mann-Whitney no pareada. Como resultado, se encontró que el área de superficie media de los valores de patrón de grabado de tipo I y II para el Grupo A era 1922,314 µm2 y el Grupo B era 3840,473 µm2. Finalmente, llegamos a la conclusión de que se puede usar la desproteinización con NaOCl 5% antes del grabado ácido para aumentar el área de adhesión y la calidad del patrón de grabado.

Comparative study about the factors associated with parental dental anxiety in a peruvian hospital / Estudio comparativo de factores asociados con la ansiedad dental parental en un hospital peruano

Background: Dental anxiety is a source of problems in the dental treatment that can lead to dental avoidance. Objective: Determine and compare the factors associated with dental anxiety in Peruvian parents. Material and Methods: An observational, descriptive and cross-sectional study was carried out. The sample consisted of children from 3 to 6 years old and their companions, who attended the Instituto Nacional de Salud del Niño in Lima, Peru. The Corah anxiety scale, the dental fear questionnaire and the Frankl scale were used to assess anxiety, parental dental fear, and children's collaboration. The following variables were also evaluated: negative dental experience, treated tooth and treatment of the children. Chi-square statistical tests, Mann-Whitney U and the binary logistic regression model were used. Results: The sample consisted of 325 children and their companions. An association was found between gender (OR = 2.456; 95% CI: 1.037 - 5.818), child collaboration (OR = 0.044; 95% CI: 0.044 - 0.543) and maternal anxiety (p<0.05). Furthermore, there was an association between dental fear (OR = 3.569; 95% CI: 1.136 - 11.218), child collaboration (OR = 0.023; 95% CI: 0.003 - 0.172) and paternal anxiety (p<0.05). Conclusion: The factor associated with maternal dental anxiety was the sex of the child, and on the father's side, it was the dental fear experienced by them. Moreover, the component affiliated with both parents was the child's collaboration.

Introducción: La ansiedad dental es una fuente de problemas en el tratamiento odontológico que puede conducir a la evitación dental. Objetivo: Determinar y comparar los factores asociados a la ansiedad dental en padres peruanos. Material y Métodos: Se realizó un estudio observacional, descriptivo y transversal. La muestra estuvo compuesta por niños de 3 a 6 años de edad y sus acompañantes que asistieron al Instituto Nacional de Salud del Niño en Lima, Perú. Se usó la escala de ansiedad de Corah, el cuestionario de miedos dentales y la escala de Frankl para evaluar ansiedad, miedo dental parental y colaboración de los niños respectivamente. También se evaluaron las siguientes variables: experiencia dental negativa, pieza dental tratada y tratamiento de los niños. Se utilizaron las pruebas estadísticas de Chi-cuadrado, U de Mann-Whitney y el modelo de regresión logística binaria. Resultados: La muestra estuvo conformada por 325 niños y sus acompañantes. Se encontró una asociación entre el sexo (OR =2.456; 95%IC: 1.037 ­ 5.818), colaboración del niño (OR=0.044; 95%IC: 0.044 ­ 0.543) y la ansiedad materna (p<0.05). Se encontró una asociación entre el miedo dental (OR= 3.569; 95% IC: 1.136 ­ 11.218), colaboración del niño (OR= 0.023; 95% IC: 0.003 ­ 0.172) y la ansiedad paterna (p<0.05). Conclusión: El factor asociado a la ansiedad dental materna fue el sexo del niño, y en el padre, fue el miedo dental experimentado por el mismo. El factor asociado a ambos padres fue la colaboración del niño.

IgA-coated E. coli enriched in Crohn's disease spondyloarthritis promote TH17-dependent inflammation.

Peripheral spondyloarthritis (SpA) is a common extraintestinal manifestation in patients with active inflammatory bowel disease (IBD) characterized by inflammatory enthesitis, dactylitis, or synovitis of nonaxial joints. However, a mechanistic understanding of the link between intestinal inflammation and SpA has yet to emerge. We evaluated and functionally characterized the fecal microbiome of IBD patients with or without peripheral SpA. Coupling the sorting of immunoglobulin A (IgA)-coated microbiota with 16S ribosomal RNA-based analysis (IgA-seq) revealed a selective enrichment in IgA-coated Escherichia coli in patients with Crohn's disease-associated SpA (CD-SpA) compared to CD alone. E. coli isolates from CD-SpA-derived IgA-coated bacteria were similar in genotype and phenotype to an adherent-invasive E. coli (AIEC) pathotype. In comparison to non-AIEC E. coli, colonization of germ-free mice with CD-SpA E. coli isolates induced T helper 17 cell (TH17) mucosal immunity, which required the virulence-associated metabolic enzyme propanediol dehydratase (pduC). Modeling the increase in mucosal and systemic TH17 immunity we observed in CD-SpA patients, colonization of interleukin-10-deficient or K/BxN mice with CD-SpA-derived E. coli lead to more severe colitis or inflammatory arthritis, respectively. Collectively, these data reveal the power of IgA-seq to identify immunoreactive resident pathosymbionts that link mucosal and systemic TH17-dependent inflammation and offer microbial and immunophenotype stratification of CD-SpA that may guide medical and biologic therapy.

Estimación de la conicidad de los conductos radiculares de primeros molares inferiores primarios utilizando Nano tomografía computarizada (Nano-CT): Estudio in vitro

Objetivo: Estimar la conicidad de conductos radiculares de primeros molares inferiores primarios por medio de Nano tomografía computarizada (Nano-CT). Material y Métodos: La muestra consistió en 10 dientes molares inferiores primarios. Los dientes fueron escaneados por medio de Nano-CT y las imágenes resultantes fueron analizadas con el software On-Demand 3D, considerando entre la evaluación las características anatómicas: la dimensión de los conductos radiculares y área de superficie de los mismos en toda la longitud radicular. Para la medida del mayor y menor diámetro de los conductos radiculares los test estadísticos fueron medidas de tendencia central, con un nivel de significancia de 5%. Resultados: En la raíz distal, mesio vestibular y mesio lingual la dimensión del conducto radicular con mayor promedio de la región cervical (D0) fue de 1.30, 1.00, 1.08 mm, respectivamente. El diámetro a la longitud más profunda (D11) para los mismos conductos fue de 0.29, 0.22, 0.18 mm. Mientras, la medida del área de superficie radicular distal, mesio vestibular y mesio lingual en la línea cervical (D0) fue de 1.43, 0.87, 1.01 mm. El área a la longitud más profunda (D11) fue de 0.09, 0.04, 0.03 mm. Conclusiones: La morfología de las raíces y de los conductos radiculares de primeros molares primarios presentaron una distribución cónica siendo sus diámetros y áreas mayor en la región cervical y disminuyen en la región apical.

Uso de crema dental en niños peruanos menores de 12 años, durante el periodo del 2016 al 2018. Resultados de una encuesta nacional

Objetivo: Reportar el uso crema dental en niños peruanos menores de 12 años, durante el periodo del 2016 - 2018. Materiales y métodos: El estudio fue descriptivo, longitudinal y retrospectivo, utilizándose los registros de la base de datos ENDES, Perú, durante 2016-2018. La muestra fue multietápico, probabilística y estratificada, formada por 118 716 registros de niños menores de 12 años de edad. Las variables fueron: uso de crema dental, concentración de flúor, departamento, ámbito geográfico y región natural. Se realizó un análisis descriptivo de las frecuencias absolutas y relativas. Resultados: De los participantes, se cepillaban los dientes, el 88%; se cepillan los dientes 2 veces al día, el 40%; utilizaban crema dental, el 98%; usaban crema dental con una concentración de flúor de 1000ppm o más, el 44,6% (n=15 376), 43,2% (n=14 545) y 44% (n=16 227), para los años 2016, 2017 y 2018, respectivamente. En los departamentos de Piura, Loreto, San Martin y Ucayali usan crema dental ≥1000ppm de flúor, en mayor porcentaje, en un 55% a 74,9%. Las cremas dentales con ≥1000ppm de flúor fueron más utilizadas en la región de la selva y zonas urbanas. Conclusión: Se encontró que la población se cepilla los dientes, con una frecuencia de 2 veces al día y utilizan crema dental; sin embargo, menos de la mitad de peruanos menores de 12 años de edad, utiliza crema dental fluorada con 1000 ppm o más. Estos resultados se podrían relacionarse con las políticas preventivas actuales contra la caries dental.

CX3CR1⁺ mononuclear phagocytes support colitis-associated innate lymphoid cell production of IL-22.

Interleukin (IL)-22-producing group 3 innate lymphoid cells (ILC3) promote mucosal healing and maintain barrier integrity, but how microbial signals are integrated to regulate mucosal protection offered by these cells remains unclear. Here, we show that in vivo depletion of CX3CR1⁺ mononuclear phagocytes (MNPs) resulted in more severe colitis and death after infection with Citrobacter rodentium. This phenotype was rescued by exogenous IL-22, which was endogenously produced by ILC3 in close spatial proximity to CX3CR1⁺ MNPs that were dependent on MyD88 signaling. CX3CR1⁺MNPs from both mouse and human tissue produced more IL-23 and IL-1ß than conventional CD103(+) dendritic cells (cDCs) and were more efficient than cDCs in supporting IL-22 production in ILC3 in vitro and in vivo. Further, colonic ILC3 from patients with mild to moderate ulcerative colitis or Crohn's disease had increased IL-22 production. IBD-associated SNP gene set analysis revealed enrichment for genes selectively expressed in human intestinal MNPs. The product of one of these, TL1A, potently enhanced IL-23- and IL-1ß-induced production of IL-22 and GM-CSF by ILC3. Collectively, these results reveal a critical role for CX3CR1⁺ mononuclear phagocytes in integrating microbial signals to regulate colonic ILC3 function in IBD.