Identification and characterisation of small-molecule inhibitors of Rv3097c-encoded lipase (LipY) of Mycobacterium tuberculosis that selectively inhibit growth of bacilli in hypoxia.

The mycobacterial Rv3097c-encoded lipase LipY is considered as a true lipase involved in the hydrolysis of triacylglycerol stored in lipid inclusion bodies for the survival of dormant mycobacteria. To date, orlistat is the only known LipY inhibitor. In view of the important emerging role of this enzyme, a search for small-molecule inhibitors of LipY was made, leading to the identification of some new compounds (8a-8d, 8f, 8h and 8i) with potent inhibitory activities against recombinant LipY, with no cytotoxicity [50% inhibitory concentration (CC(50)) ≥ 500 µg/mL]. The compounds 6a, 8c and 8f potently inhibited (>90%) the growth of Mycobacterium tuberculosis H37Rv grown under hypoxia (oxygen-depleted condition) but had no effect on aerobically grown bacilli, suggesting that these new small molecules are highly selective towards the growth inhibition of hypoxic cultures of M. tuberculosis and hence provide new leads for combating latent tuberculosis.

New Cycloartenol Derivatives from Aglaia roxburghiana1.

Two triterpenes, isolated from the aerial parts of AGLAIA ROXBURGHIANA have been characterized as 29-nor-cycloartan-24,25-epoxy-3beta-ol and 29-nor-cycloartan-23-ene-3beta,25-diol; 29-nor-cycloartenol and 28,29-bis-nor-cycloartan-24-methylene-3beta,6alpha-diol, were also isolated.

Antiviral activity of (+)-odorinol1.

A phytochemical investigation of AGLAIA ROXBURGHIANA var. Beddomei (Meliaceae) resulted in the isolation of the active principle and its characterisation as (+)-odorinol exhibiting strong antiviral activity against Ranikhet disease virus (RVD) in chick embryo.