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1.
Clin Radiol ; 76(7): 549.e1-549.e8, 2021 07.
Artigo em Inglês | MEDLINE | ID: mdl-33888302

RESUMO

AIM: To assess the role of a severity score based on chest radiography (CXR) in predicting the risk of adverse outcomes in coronavirus disease 2019 (COVID-19). MATERIALS AND METHODS: Of the patients who presented to L. Sacco Hospital (Milan, Italy) between 21 February and 31 March 2020, patients with a laboratory confirmation of COVID-19 who also underwent a CXR were included in the study. To quantify the extent of lung involvement, each CXR image was given a score (Milan score), ranging from 0 to 24, depending on the presence of reticular pattern and/or ground-glass opacities and/or extensive consolidations in each of the 12 areas in which the lungs were divided. The score was calculated by an expert radiologist, blinded to laboratory tests. The ability of the Milan score to predict hospital admission and mortality, after adjusting for some variables (age; gender; comorbidities; time between symptoms onset and admission), using univariate and multivariate statistical analysis was investigated retrospectively. RESULTS: Among the 554 patients, 115 of which (21%) had a negative CXR, the in-hospital mortality was 16% (90/554). At univariate analysis, age, gender, and comorbidities were significant predictors of mortality and hospital admission. At multivariate analysis, adjusting for age and gender, the Milan score was an independent predictor of mortality and hospitalisation. In particular, patients with a Milan score ≥ 9 had a mortality risk five-times higher than those with a lower score. Other independent predictors of mortality were gender and age. CONCLUSIONS: The CXR Milan score was an independent predictive factor of both in-hospital mortality and hospital admission.


Assuntos
COVID-19/diagnóstico por imagem , COVID-19/epidemiologia , Mortalidade Hospitalar , Radiografia Torácica/métodos , Fatores Etários , Idoso , Feminino , Humanos , Itália/epidemiologia , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Estudos Retrospectivos , SARS-CoV-2 , Índice de Gravidade de Doença , Fatores Sexuais
2.
Artigo em Inglês | MEDLINE | ID: mdl-29581111

RESUMO

Our in vitro studies showed that a combination of amphotericin B and terbinafine had synergistic effects against the majority of melanized fungi associated with chromoblastomycosis (CBM) and similar infections, including those with Cladophialophora carrionii, Cladophialophora arxii, Exophialadermatitidis, Exophialaspinifera, Fonsecaea monophora, Fonsecaea nubica, Fonsecaea pedrosoi, and Phialophora verrucosa. This drug combination could provide an option for the treatment of severe or unresponsive cases of CBM, particularly in cases due to species of Fonsecaea and Cladophialophora.


Assuntos
Anfotericina B/farmacologia , Antifúngicos/farmacologia , Cromoblastomicose/microbiologia , Terbinafina/farmacologia , Ascomicetos/efeitos dos fármacos , Exophiala/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Phialophora/efeitos dos fármacos
3.
Antimicrob Agents Chemother ; 60(4): 2346-51, 2016 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-26833164

RESUMO

Primary central nervous system phaeohyphomycosis is a fatal fungal infection due mainly to the neurotropic melanized fungiCladophialophora bantiana,Rhinocladiella mackenziei, andExophiala dermatitidis.Despite the combination of surgery with antifungal treatment, the prognosis continues to be poor, with mortality rates ranging from 50 to 70%. Therefore, a search for a more-appropriate therapeutic approach is urgently needed. Ourin vitrostudies showed that with the combination of amphotericin B and flucytosine against these species, the median fractional inhibitory concentration (FIC) indices for strains ranged from 0.25 to 0.38, indicating synergy. By use of Bliss independence analysis, a significant degree of synergy was confirmed for all strains, with the sum ΔE ranging from 90.2 to 698.61%. No antagonism was observed. These results indicate that amphotericin B, in combination with flucytosine, may have a role in the treatment of primary cerebral infections caused by melanized fungi belonging to the orderChaetothyriales Furtherin vivostudies and clinical investigations to elucidate and confirm these observations are warranted.


Assuntos
Anfotericina B/farmacologia , Antifúngicos/farmacologia , Exophiala/efeitos dos fármacos , Flucitosina/farmacologia , Saccharomycetales/efeitos dos fármacos , Feoifomicose Cerebral/microbiologia , Feoifomicose Cerebral/patologia , Meios de Cultura/química , Combinação de Medicamentos , Sinergismo Farmacológico , Exophiala/crescimento & desenvolvimento , Exophiala/isolamento & purificação , Exophiala/patogenicidade , Análise Fatorial , Humanos , Testes de Sensibilidade Microbiana , Saccharomycetales/crescimento & desenvolvimento , Saccharomycetales/isolamento & purificação , Saccharomycetales/patogenicidade
4.
Biochim Biophys Acta ; 1831(4): 872-9, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-23354175

RESUMO

A number of viruses contain lipid membranes, which are in close contact with capsid proteins and/or nucleic acids and have an important role in the viral infection process. In this study membrane lipids of intact viruses have been analysed by MALDI-TOF/MS with a novel methodology avoiding lipid extraction and separation steps. To validate the novel method, a wide screening of viral lipids has been performed analysing highly purified intact bacterial and archaeal viruses displaying different virion architectures. Lipid profiles reported here contain all lipids previously detected by mass spectrometry analyses of virus lipid extracts. Novel details on the membrane lipid composition of selected viruses have also been obtained. In addition we show that this technique allows the study of lipid distribution easily in subviral particles during virus fractionation. The possibility to reliably analyse minute amounts of intact viruses by mass spectrometry opens new perspectives in analytical and functional lipid studies on a wider range of viruses including pathogenic human ones, which are difficult to purify in large amounts.


Assuntos
Lipídeos/análise , Lipídeos/química , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos , Vírus/química , Modelos Biológicos
5.
Eur J Surg Oncol ; 50(9): 108471, 2024 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-39002445

RESUMO

INTRODUCTION: Non palpable breast tumors are increasingly diagnosed because of screening programs, and their localization during surgery is essential to ensure an adequate resection. Little is known regarding which techniques are used in "real world". MATERIALS AND METHODS: A national web-based survey, with a 21-items questionnaire, was conducted among Breast Centers (BCs) in Italy in 2023. RESULTS: Among 153 BCs, 110 (72 %) participated. Wire-guided localization was reported to be the most used technique, regardless of the volume of malignant tumors treated by each Center (N = 36, 33 %). However, newer techniques such as Radioactive occult lesion localization and magnetic seeds, were reported to be employed in 34 (31 %) BCs, and more often among high-volume BCs (>300 cases/year) (N = 32, 29 % vs. N = 13, 12 %; p = 0.02). Logistic problems of localization were reported to cause delays to the scheduled surgery at least once or in multiple occasions in 26 (24 %) and in 4 (3.5 %) BCs, respectively. Although the majority of BCs declared they were satisfied (N = 48, 44 %) or somewhat satisfied (N = 41, 37 %) with the procedure used in their center, responders stated that they would change their technique, or that they were strongly considering this possibility in 24 (22 %) and in 38 (35 %) cases, respectively. The main barrier to introducing a new technique was associated with procedure costs (90 BCs, 82 %). CONCLUSIONS: There are several critical issues regarding localization techniques at a national level. This should be recognized as a priority because of its impact on both patients and clinical practices.


Assuntos
Neoplasias da Mama , Humanos , Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/cirurgia , Itália , Feminino , Inquéritos e Questionários , Mastectomia Segmentar , Marcadores Fiduciais
6.
J Viral Hepat ; 20(3): 200-8, 2013 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-23383659

RESUMO

Viral hepatitis reactivation has been widely reported in patients undergoing immunosuppressive therapy; however, few data are available about the risk of HBV and HCV reactivation in patients with inflammatory bowel disease, receiving immunosuppressive drugs. The aim of our study was to assess the prevalence of HBV and HCV infection in a consecutive series of patients with inflammatory bowel disease and to value the effects of immunosuppressive therapy during the course of the infection. Retrospective observational multicenter study included all consecutive patients with inflammatory bowel disease who have attended seven Italian tertiary referral hospitals in the last decade. A total of 5096 patients were consecutively included: 2485 Crohn's disease and 2611 Ulcerative Colitis. 30.5% and 29.7% of the patients were investigated for HBV and HCV infection. A total of 30 HBsAg positive, 17 isolated anti-HBc and 60 anti-HCV-positive patients were identified. In all, 20 patients with HBV or HCV infection received immunosuppressive therapy (six HBsAg+; four isolated anti-HBc+ and 10 anti-HCV+). One of six patients showed HBsAg+ and one of four isolated anti-HBc+ experienced reactivation of hepatitis. Two of six HBsAg patients received prophylactic therapy with lamivudine. Only one of 10 anti-HCV+ patients showed mild increase in viral load and ALT elevation. Screening procedures for HBV and HCV infection at diagnosis have been underused in patients with inflammatory bowel disease. We confirm the role of immunosuppressive therapy in HBV reactivation, but the impact on clinical course seems to be less relevant than previous reported.


Assuntos
Hepatite B Crônica/complicações , Hepatite C Crônica/complicações , Imunossupressores/administração & dosagem , Doenças Inflamatórias Intestinais/complicações , Doenças Inflamatórias Intestinais/tratamento farmacológico , Adolescente , Adulto , Idoso , Alanina Transaminase/sangue , Feminino , Hepacivirus/isolamento & purificação , Vírus da Hepatite B/isolamento & purificação , Humanos , Itália , Masculino , Pessoa de Meia-Idade , Estudos Multicêntricos como Assunto , Estudos Retrospectivos , Centros de Atenção Terciária , Carga Viral , Ativação Viral/efeitos dos fármacos , Adulto Jovem
7.
Braz J Microbiol ; 53(2): 759-775, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35315001

RESUMO

In 2019, severe acute respiratory syndrome caused by CoV-2 virus became a pandemic worldwide, being the fast spread of the disease due to the movement of infected people from one country to another, from one continent to another, or within the same country. Associated comorbidities are important factors that predispose to any fungal coinfections. Because of the importance of fungal infections in COVID-19 patients, the aim of this work was to collect data of the more encountered mycoses related to patients undergoing this disease. Aspergillosis was the first COVID-19-related fungal infection reported, being A. fumigatus the most frequent species for CAPA. Other fungal infections related include mainly candidiasis and mucormycosis, being Rhizopus spp. the more prevalent species found. Influenza-associated pulmonary aspergillosis is well documented; thus, similar complications are expected in severe forms of COVID-19 pneumonia. Therefore, in patients with COVID-19, it is important to take special attention to the surveillance and suspicion of fungal coinfections that might worsen the patient's prognosis.


Assuntos
COVID-19 , Coinfecção , Micoses , COVID-19/epidemiologia , Coinfecção/epidemiologia , Humanos , Micoses/epidemiologia , Pandemias , SARS-CoV-2
8.
Biomed Pharmacother ; 156: 113844, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36252359

RESUMO

The 2-pentadecyl-2-oxazoline (PEA-OXA) is a natural compound with protective action in neuro-inflammation. We have previously shown that PEA-OXA behaves as an α2 adrenergic receptor (α2AR) antagonist and a putative protean agonist on histamine H3 receptors. Recently, neuroinflammation and monoaminergic neurotransmission dysfunction has drawn particular attention in Alzheimer Disease (AD) pathophysiology. In this context, the objective of this study was to investigate the effects of the dual-acting PEA-OXA in an AD-like model in mice. A combined computational and experimental approach was used to evaluate the ability of PEA-OXA to bind α2A-AR subtype, and to investigate the effects of PEA-OXA treatment on neuropathological (behavioural and functional) effects induced by soluble Amyloid ß 1-42 (sAß1-42) intracerebroventricular injection. Computational analysis revealed the PEA-OXA ability to bind the α2A-AR, a pharmacological target for AD, in two alternative poses, one overlapping the Na+ binding site. In vivo studies indicated that chronic treatment with PEA-OXA (10 mg/kg, os) restored the cognitive (discriminative and spatial memory) deficits and social impairments induced by sAß injection. Consistently, electrophysiological analysis showed a recovery of the long-term potentiation in the hippocampus (Lateral Entorhinal Cortex-Dentate Gyrus pathway), while neuroinflammation, i.e., increased pro-inflammatory cytokines levels and microglia cells density were reduced. These data provide the basis for further investigation of the pro-cognitive aptitude of PEA-OXA by proposing it as an adjuvant in the treatment in AD, for which the available pharmacological approaches remain unsatisfactory. Moreover, this study offers new future direction in research investigating the role of α2AR in neuropsychiatric illness and therapies.


Assuntos
Doença de Alzheimer , Peptídeos beta-Amiloides , Camundongos , Animais , Peptídeos beta-Amiloides/toxicidade , Peptídeos beta-Amiloides/metabolismo , Doença de Alzheimer/tratamento farmacológico , Doença de Alzheimer/prevenção & controle , Receptores Adrenérgicos alfa 2 , Modelos Animais de Doenças , Comportamento Social , Cognição
9.
Environ Technol ; 31(8-9): 935-42, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20662382

RESUMO

In this work the thermophilic trehalose/maltose-binding protein from Thermococcus litoralis is presented as a probe for the design of a high stable fluorescence biosensor for glucose. In particular, we show the possibility of modulating the protein specificity by changing temperature. In addition to glucose sensing, we also report on the possibility of utilizing odorant-binding proteins as a probe for the development of optical sensors for analytes of environmental interests.


Assuntos
Técnicas Biossensoriais/métodos , Glucose/análise , Nanotecnologia/métodos , Proteínas Periplásmicas de Ligação/metabolismo , Thermococcus/metabolismo , Trealose/metabolismo , Proteínas Ligantes de Maltose , Modelos Moleculares , Conformação Proteica , Espectrometria de Fluorescência , Temperatura
10.
Rev Argent Microbiol ; 42(4): 261-8, 2010.
Artigo em Espanhol | MEDLINE | ID: mdl-21229194

RESUMO

Clinical cases of coccidioidomycosis are rare in Argentina and are generally found in the large arid precordilleran area of the country. This study aims to perform a retrospective review of all coccidioidomycosis cases documented in the country from 1892 to 2009, and to describe those occurring in the last 4 years. One hundred and twenty eight cases were documented in the 117 year-period. Since the original description of the disease in 1892 until 1939, only 6 cases were registered; between 1940 and 1999, 59 (6-14/10 yrs) and the remaining 63 (49% of total cases) occurred in the last decade. The median age of 34 patients registered in 2006-2009 was 31 years (range: 7-89), male/female ratio was 1.3:1 and 12 patients were immunocompromised. Twenty-six cases were confirmed by direct microscopy and/or culture whereas the remaining ones by serology. All isolates were identified as Coccidioides posadasii. Thirty patients lived in a vast geographic region with epicenter in Catamarca Valley. Between 2006 and 2009, annual disease incidence rates in Catamarca Province increased from historical values below 0.5/100,000 to 2/100,000 inhabitants. Such increase suggests an emergency of coccidioidomycosis in that region.


Assuntos
Coccidioidomicose/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Criança , Coccidioidomicose/diagnóstico , Coccidioidomicose/parasitologia , Erros de Diagnóstico , Feminino , Humanos , Hospedeiro Imunocomprometido , Incidência , Masculino , Pessoa de Meia-Idade , Morbidade/tendências , Estudos Retrospectivos , Estudos Soroepidemiológicos , Tuberculose/diagnóstico , Adulto Jovem
11.
Anal Chim Acta ; 1125: 308-314, 2020 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-32674778

RESUMO

Ripening is a crucial step to guarantee the high commercial value of cheddar cheese, one of the dairy products the European Union exports the most. Although several methods have lately been proposed to assess its ageing process from a chemical point of view, the majority of them is not particularly time-efficient and implies destructive analytical tests, thus, exhibiting limitations for, e.g., industrial applications. Here, a fast approach based on combining Raman and Mid-InfraRed (MIR) spectroscopy with ANOVA-Simultaneous Component Analysis (ASCA) is proposed in a low-level data fusion framework. This approach allowed to evaluate how storage temperature and time (as well as their interaction) influence cheddar ripening in a relatively cheap, rapid and green fashion.


Assuntos
Queijo/análise , Análise de Variância , Espectrofotometria Infravermelho/estatística & dados numéricos , Análise Espectral Raman , Temperatura , Fatores de Tempo
12.
Rev Argent Microbiol ; 39(4): 218-20, 2007.
Artigo em Espanhol | MEDLINE | ID: mdl-18390155

RESUMO

A clinical case of a female patient with a black spot on the palm of her left hand is presented. The infection was due to a black fungus identified as Hortaea werneckii, the aetiological agent of tinea nigra palmaris. This infection can be easily diagnosed and it is important to establish the differential diagnosis from other skin pathologies. Normally, the treatment has a successful outcome. In this case, the patient was treated with econazole locally applied during one month, with complete remission of the lesions. In conclusion, the early diagnosis of this disease is very important since the mycology procedures are fast and non-invasive and cure is obtained with local treatment.


Assuntos
Dermatoses da Mão/diagnóstico , Tinha/diagnóstico , Antifúngicos/uso terapêutico , Econazol/uso terapêutico , Feminino , Dermatoses da Mão/tratamento farmacológico , Dermatoses da Mão/microbiologia , Humanos , Pessoa de Meia-Idade , Fungos Mitospóricos/isolamento & purificação , Tinha/tratamento farmacológico , Tinha/microbiologia
13.
Radiol Res Pract ; 2017: 2761818, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28567306

RESUMO

The aim of this study was to assess with MRI morphometric ultrastructural changes in nerves affected by diabetic peripheral neuropathy (DPN). We used an MR micro-neurography imaging protocol and a semiautomated technique of tissue segmentation to visualize and measure the volume of internal nerve components, such as the epineurium and nerve fascicles. The tibial nerves of 16 patients affected by DPN and of 15 healthy volunteers were imaged. Nerves volume (NV), fascicles volume (FV), fascicles to nerve ratio (FNR), and nerves cross-sectional areas (CSA) were obtained. In patients with DPN the NV was increased and the FNR was decreased, as a result of an increase of the epineurium (FNR in diabetic neuropathy 0,665; in controls 0,699, p = 0,040). CSA was increased in subjects with DPN (12,84 mm2 versus 10,22 mm2, p = 0,003). The FV was increased in patients with moderate to severe DPN. We have demonstrated structural changes occurring in nerves affected by DPN, which otherwise are assessable only with an invasive biopsy. MR micro-neurography appears to be suitable for the study of microscopic changes in tibial nerves of diabetic patients.

14.
Endocrinology ; 97(3): 636-48, 1975 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-1175511

RESUMO

Androgen binding protein (ABP) was measured during postnatal development in normal and irradiated rats to determine whether development of a blood-testis barrier and formation of a continuous lumen from testis to epididymis is correlated with entry of ABP into the caput epididymis. ABP is found in normal testis as early as 14 days postnatally (0.2 pmol/mg), at which time no blood-testis barrier is observed by the peroxidase perfusion technique. Previous findings have shown a close correlation of blood-testis barrier development and lumen formation. Indeed, ABP is not detectable in the epididymis until 18-20 days of age (1.0 pmol/mg) at which time blood-testis barrier formation and lumen development is complete. Whole body irradiation (125 rads) of pregnant rats at 19-20 days of gestation produces male offspring with seminiferous tubules remarkably free of germinal epithelium and containing essentially only Sertoli cells. These Sertoli cell-enriched (SCE) testes produce normal amounts of ABP between 14 and 21 days postnatally. However, between 21 and 30 days of age the specific activity of ABP is significantly higher in the SCE tests (2.8 pmol/mg protein) than in normal testis (0.5 pmol/mg protein). In the SCE testis neither blood-testis barrier development nor lumen formation are complete until 30 days of age, at which time ABP is first detectable in epididymis of the irradiated rat. Thereafter there is a gradual decline of ABP in the SCE testis-and a dramatic increase in the epididymis. NIH-FSH-S-10 (200 mug/rat) injected SC into 14-day-old normal rats stimulated ABP in the testis from control levels of 0.15 pmol/mg to 1.46 pmol/mg within 4 h after injection. However, no ABP was detectable in the epididymis of either the control or the FSH-stimulated rats. These findings suggest that entry of ABP into caput epididymis is an index of blood-testis barrier formation and lumen development.


Assuntos
Androgênios/metabolismo , Barreira Hematotesticular , Proteínas de Transporte/metabolismo , Testículo/crescimento & desenvolvimento , Animais , Diferenciação Celular , Epididimo/metabolismo , Feminino , Hormônio Foliculoestimulante/metabolismo , Masculino , Gravidez , Ratos , Testículo/ultraestrutura
15.
Qual Saf Health Care ; 13(4): 265-71, 2004 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-15289629

RESUMO

OBJECTIVES: A set of standard processes was developed for delivering continuous drug infusions in order to improve (1) patient safety; (2) efficiency in staff workflow; (3) hemodynamic stability during infusion changes, and (4) efficient use of resources. Failure modes effects analysis (FMEA) was used to examine the impact of process changes on the reliability of delivering drug infusions. SETTING: An 11 bed multidisciplinary pediatric ICU in the children's hospital of an academic medical center staffed by board certified pediatric intensivists. The hospital uses computerized physician order entry for all medication orders. METHODS: A multidisciplinary team characterized key elements of the drug infusion process. The process was enhanced to increase overall reliability and the original and revised processes were compared using FMEA. Resource consumption was estimated by reviewing purchasing and pharmacy records for the calendar year after full implementation of the revised process. Staff satisfaction was evaluated using an anonymous questionnaire administered to staff nurses in the ICU and pediatric residents who had rotated through the ICU. RESULTS: The original process was characterized by six elements: selecting the drug; selecting a dose; selecting an infusion rate; calculating and ordering the infusion; preparing the infusion; programming the infusion pump and delivering the infusion. The following practice changes were introduced: standardizing formulations for all infusions; developing database driven calculators; extending infusion hang times from 24 to 72 hours; changing from bedside preparation by nurses to pharmacy prepared or premanufactured solutions. FMEA showed that the last three elements of the original process had high risk priority numbers (RPNs) of >225 whereas the revised process had no elements with RPNs >100. The combined effect of prolonging infusion hang times, preparation in the pharmacy, and purchasing premanufactured solutions resulted in 1500 fewer infusions prepared by nurses per year. Nursing staff expressed a significant preference and pediatric residents unanimously expressed a strong preference for the revised process. CONCLUSIONS: Standardization of infusion delivery reduced the frequency for completing the most unreliable elements of the process and reduced the riskiness of the individual elements. Both contribute to a safer system.


Assuntos
Infusões Intravenosas/normas , Unidades de Terapia Intensiva Pediátrica/normas , Erros de Medicação/prevenção & controle , Sistemas de Medicação no Hospital/normas , Gestão da Segurança , Centros Médicos Acadêmicos , Adolescente , Criança , Pré-Escolar , Sistemas de Informação em Farmácia Clínica , Connecticut , Quimioterapia Assistida por Computador , Registros Hospitalares , Hospitais Pediátricos/normas , Humanos , Equipe de Assistência ao Paciente , Avaliação de Processos em Cuidados de Saúde , Análise de Sistemas , Gestão da Qualidade Total
17.
Medicina (B Aires) ; 50(4): 291-8, 1990.
Artigo em Espanhol | MEDLINE | ID: mdl-2130222

RESUMO

We have evaluated the results of differentiated thyroid carcinoma treatment after 15 years of follow up. The group consisted of 120 cases of papillary type and 144 cases of follicular type; the mean age of the patients was 38.2 years for papillary and 46.5 for the follicular. The ratio men/women was 1/4 for the papillary and 1/3.2 for the follicular. The prognostic factors evaluated included: a) histologic types according to WHO classification, b) clinical stage, c) age and d) sex. The staging of the disease followed the classification suggested by Smendal, stage I: intraglandular disease; stage II: regional lymph node metastases; stage III: invasion of other tissues in the neck or mediastinum and stage IV: distant metastases. The basic treatment was surgery with the use of 131I and thyroid hormone. The results were statistically analyzed with the conventional life table method or chi square. At 15 years the overall survival was 84 +/- 4% for the papillary type and 66 +/- 9% for the follicular type (p = 0.01) (Fig. 1). The patients treated in stage I had a survival of 90 +/- 5%, significantly higher than those in stage II (53 +/- 8%), p less than 0.03. In stage III and IV the small number of patients made it difficult to interpret the results statistically (Fig. 2). The age of the patients appears to be another prognostic factor. The group under 40 years had 85 +/- 5% of survival compared to 59 +/- 4% over 40 years, p less than 0.01 (Fig. 3).(ABSTRACT TRUNCATED AT 250 WORDS)


Assuntos
Neoplasias da Glândula Tireoide/terapia , Adulto , Fatores Etários , Feminino , Humanos , Masculino , Estadiamento de Neoplasias , Prognóstico , Recidiva , Estudos Retrospectivos , Fatores Sexuais , Neoplasias da Glândula Tireoide/mortalidade , Tireoidectomia
18.
Rev Argent Microbiol ; 29(1): 7-15, 1997.
Artigo em Espanhol | MEDLINE | ID: mdl-9229726

RESUMO

Candidiasis has increased its frequency over the last decade, particularly among hospitalized patients where it is accompanied with high rates of mortality, and in patients with AIDS who are predisposed to oropharyngeal or esophageal candidiasis. The aim of this study was to determine the frequency of appearance of different yeast species and the resistance profile to current antifungal drugs in hospitalized pediatric patients and adult HIV patients from 5 institutions of Buenos Aires City and suburbs, and La Plata City, during the period 1993-1995, in order to obtain local and updated information. Candida albicans was the etiologic agent recovered in 87% of the 214 HIV positive patients with oropharyngeal candidosis, and in 50% of the 209 hospitalized pediatric patients. In the latter group 28% of these infections were due to Candida parapsilosis and 18% to Candida tropicalis, but only 2% and 4% of oral candidosis were caused by these organisms. Detection of Malassezia furfur and Hansenula anomala, responsible of systemic infections, and Trichosporon beigelii, isolated from a burn patient, were considered remarkable since these organisms appear to be emerging pathogens. Azole resistant species as Candida krusei and C. glabrata were mostly recovered from HIV positive patients, exposed to fluconazole treatment. A very low number of amfotericin B "resistant" yeasts (n = 9) were observed in both groups. However, resistance to azole drugs, particularly to fluconazole, was found in pediatric patients (13%) and in HIV infected adults (34%).


Assuntos
Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Antifúngicos/farmacologia , Micoses/microbiologia , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Adolescente , Adulto , Anfotericina B/farmacologia , Argentina/epidemiologia , Candida/efeitos dos fármacos , Candida/isolamento & purificação , Candidíase Bucal/epidemiologia , Candidíase Bucal/microbiologia , Criança , Pré-Escolar , Infecção Hospitalar/epidemiologia , Infecção Hospitalar/microbiologia , Resistência Microbiana a Medicamentos , Feminino , Humanos , Lactente , Recém-Nascido , Cetoconazol/farmacologia , Masculino , Pessoa de Meia-Idade , Micoses/epidemiologia , Pichia/efeitos dos fármacos , Pichia/isolamento & purificação , Estudos Retrospectivos , Triazóis/farmacologia , Leveduras/efeitos dos fármacos , Leveduras/isolamento & purificação
19.
Chem Commun (Camb) ; 50(15): 1894-6, 2014 Feb 21.
Artigo em Inglês | MEDLINE | ID: mdl-24402376

RESUMO

UV-visible absorption spectroelectrochemistry elucidated the different redox behaviours of Fe(III)- and Co(III)-mimochrome VI artificial enzymes, adsorbed on mesoporous conductive films of ITO. The reduction of the ferric complex was rapid and reversible, while the cobaltic complex exhibited irreversible processes probably related to multiple coordination states.


Assuntos
Cobalto/química , Enzimas Imobilizadas/química , Ferro/química , Compostos de Estanho/química , Sequência de Aminoácidos , Eletroquímica , Eletrodos , Modelos Moleculares , Oxirredução , Porosidade , Conformação Proteica , Espectrofotometria Ultravioleta
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