In vitro interaction of Stenotrophomonas maltophilia with human monocyte-derived dendritic cells.

Stenotrophomonas maltophilia is increasingly identified as an opportunistic pathogen in immunocompromised, cancer and cystic fibrosis (CF) patients. Knowledge on innate immune responses to S. maltophilia and its potential modulation is poor. The present work investigated the ability of 12 clinical S. maltophilia strains (five from CF patients, seven from non-CF patients) and one environmental strain to survive inside human monocyte-derived dendritic cells (DCs). The effects of the bacteria on maturation of and cytokine secretion by DCs were also measured. S. maltophilia strains presented a high degree of heterogeneity in internalization and intracellular replication efficiencies as well as in the ability of S. maltophilia to interfere with normal DCs maturation. By contrast, all S. maltophilia strains were able to activate DCs, as measured by increase in the expression of surface maturation markers and proinflammatory cytokines secretion.

Clonal dissemination of Klebsiella pneumoniae ST512 carrying blaKPC-3 in a hospital in southern Italy.

Strains of Klebsiella pneumoniae producing KPC-carbapenemase have emerged as one of the most important multidrug-resistant Gram-negative nosocomial pathogens. Here, we report the first isolation and subsequent dissemination of a K. pneumoniae ST512 producing KPC-3 carbapenemase in a hospital in southern Italy. Isolates were obtained from blood, throat swabs, sputum, catheters, and urine of patients admitted to different hospital wards. Antimicrobial MICs were determined for all isolates by automated systems and confirmed by Etest. Carbapenemase production was confirmed by the modified Hodge test and by a disc synergy test, and carbapenemase genes were investigated by PCR. All isolates were characterized by pulse-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analysis. Most isolates were multidrug resistant with exception of some isolates intermediately susceptible to gentamicin, tigecycline, and trimethoprim-sulfamethoxazole. PCR analysis showed that isolates harbored the bla(KPC-3) gene associated with bla(TEM) and bla(SVH). PFGE and MLST showed that all isolates belonged to the same ST512 clone recently described in Israel.

Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus from surgical site infections.

BACKGROUND: Nosocomial infections represent an important problem for the health of hospitalized patients. Peri-operative infections--those occurring during surgery or in the post-operative period--account for 15%-20% of cases. Most surgical site infections (SSIs) are caused by endogenous gram-positive microorganisms, in particular, Staphylococcus aureus, S. epidermidis, and other coagulase-negative staphylococci that are part of the flora of the skin. METHODS: A retrospective study was conducted from January 2006 to December 2010 to describe the epidemiology of methicillin-resistant S. aureus (MRSA) in SSIs. The MRSA isolates were analyzed by a combination of two genotyping methods: SCCmec and pulsed-field gel electrophoresis (PFGE). Also, biofilm-forming ability was analyzed for all isolates as an indicator of their ability to persist despite antibiotic treatment. RESULTS: During the study period, 1,793 swabs from SSIs were analyzed, and S. aureus was identified in 318/987 positive specimens (32%). Methicillin resistance was observed in 10% of the S. aureus isolates (n=33). Analysis by PFGE revealed that isolates with the same SCCmec type were unrelated. Instead, biofilm-forming ability tests showed that SCCmec type I MRSA had the highest ability to form a film. CONCLUSIONS: The strains analyzed in our study showed a homogeneous pattern of SCCmec type. The difference in ability to produce biofilm between strains of SCCmec type I and isolates with other SCCmecs was substantial. This virulence factor could have critical implications for the formation and persistence of chronic SSIs.

Rapid and reliable MALDI-TOF mass spectrometry identification of Candida non-albicans isolates from bloodstream infections.

Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) fingerprinting has recently become an effective instrument for rapid microbiological diagnostics and in particular for identification of micro-organisms directly in a positive blood culture. The aim of the study was to evaluate a collection of 82 stored yeast isolates from bloodstream infection, by MALDI-TOF MS; 21 isolates were identified also directly from positive blood cultures and in the presence of other co-infecting micro-organisms. Of the 82 isolates grown on plates, 64 (76%) were correctly identified by the Vitek II system and 82 (100%) by MALDI-TOF MS; when the two methods gave different results, the isolate was identified by PCR. MALDI-TOF MS was unreliable in identifying two isolates (Candida glabrata and Candida parapsilosis) directly from blood culture; however, direct analysis from positive blood culture samples was fast and effective for the identification of yeast, which is of great importance for early and adequate treatment.