RESUMO
Staphylococcus aureus is an extremely infectious and malignant pathogen among many bacteria species. The aim of this work is to provide a robust classification model that would be able to identify S. aureus independent of the culture growth stage and the variations in bacteria concentration in suspension and also one that would be able to identify the pathogen among both taxonomically close species of the same genus and taxonomically distant species of different genera, using Fourier transform infrared spectroscopy (FTIR). In total, the spectra of 141 isolates of 17 bacteria have been used. Based on a combination of principal component analysis (PCA) and linear discriminant analysis (LDA), an identification model providing 100% sensitivity and 98% specificity was built. Inherent reliability and flexibility of the model have been shown. The proposed method of analysis allows us to get closer to the diagnostic requirements in the field of clinical microbiology, and it can be utilized for typing of other pathogenic bacteria species.
Assuntos
Modelos Lineares , Análise de Componente Principal , Staphylococcus aureus/isolamento & purificação , Acinetobacter baumannii/isolamento & purificação , Candida albicans/isolamento & purificação , Coagulase/metabolismo , Enterobacter cloacae/isolamento & purificação , Enterococcus faecalis/isolamento & purificação , Enterococcus faecium/isolamento & purificação , Escherichia coli/isolamento & purificação , Klebsiella pneumoniae/isolamento & purificação , Pseudomonas aeruginosa/isolamento & purificação , Serratia marcescens/isolamento & purificação , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
An Escherichia coli sequence type 31 isolate co-harbouring mcr-1 and blaNDM-1 genes on the plasmids of Incl2 and IncC groups, respectively, was recovered from a newborn with ventilator-associated pneumonia in Moscow, Russia. The convergence of polymyxin and carbapenem resistance and its expansion beyond Southeast Asia is a serious threat to human health.