RESUMO
In this paper, sphingomyelin (SM) is detected by a polyaniline hydrogel and Au nanoparticles with enzyme modified electrode (GCE/PAniH/AuNPs@enzyme). After a battery of enzymic degradation, SM can generate H2O2 and enhance the electrochemiluminescence (ECL) response of luminol, which endows the sensor with good sensitivity, specifiity and repeatability. Additionally, the proposed ECL biosensor displays good analytical performances with a wide range from 10.0 µg·mL-1 to 250.0 µg·mL-1 as well as a low detection limit of 3.50 µg·mL-1 (S/N = 3). When the ECL biosensor is used in the detection of SM in milk samples, satisfactory results are obtained, indicating that PAniH/AuNPs@enzyme will serve as a promising ECL material in the applications of H2O2-related bioassay in the future.